Polly Mitchell, Author at Nexcelom Bioscience

Product Insert: Caspase-8 Apoptosis Kit

For additional documentation, please contact support at support@nexcelom.com CaspGLOW™ Fluorescein in Active Caspase-8 Staining Kit K188-25-NK188-100-N The CaspGLOW™Fluorescein Active Caspase-8 Staining Kit offers a simple, sensitive method for detection of active Caspase-8 in living cells. When detecting caspase-8, we utilize a specific FITC-conjugated caspase-8 inhibitor, IETD-FMK. IETD-FMK is cell-permeable and binds irreversibly to active caspase-8 within the cell. The fluorescent marker (FITC) on the IETD-FMK allows for the detection of cells undergoing apoptosis via Caspase-8 activation using the Vision CBA Analysis System. Results are presented as a Caspase positive population percentage and cell concentration. Learn more about Apoptosis Reagents For [...]

By Polly Mitchell|2021-04-08T16:38:45+00:00April 8th, 2021|0 Comments

Product Insert: Caspase-3 Staining Kit for Apoptosis

Please contact support for additional documentation at support@nexcelom.com CaspGLOW™ Fluorescein in Active Caspase-3 Staining Kit K183-25-NK183-100-N The CaspGLOW™Fluorescein Active Caspase-3 Staining Kit offers a simple, sensitive method for detection of active Caspase-3 in living cells. When detecting caspase-3, we utilize a specific FITC-conjugated caspase-3 inhibitor, DEVD-FMK. DEVD-FMK is cell-permeable and binds irreversibly to active caspase-3 within the apoptotic cell. The fluorescent marker (FITC) on the FITC-DEVD-FMK allows for the detection of cells undergoing apoptosis via Caspase-3 activation using image cytometry. Each kit contains ready-to-use reagents sufficient for 100 tests along with a detailed instruction manual outlining cell staining, imaging, and [...]

By Polly Mitchell|2021-04-08T16:38:21+00:00April 8th, 2021|0 Comments

Product Insert: ViaStain Live Caspase 3/7 Detection using Hoechst

Download the Product Insert ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture with Hoechst for Celigo CSK-V0003-1 In real-time, measure apoptosis by detecting Caspase 3/7 in 2D live cell culture and 3D tumor spheroids and determine total number of cells using Hoechst. Nexcelom’s ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture kit is designed to detect caspase 3/7 activity in live cells. The ability to perform kinetic apoptosis assays allows researchers to continuously measure the caspase 3/7 activity within the cell population. This no wash assay has been shown to effectively work in both 2D and 3D cultures. The reagent (NucViewTM) [...]

By Polly Mitchell|2021-04-08T16:37:55+00:00April 8th, 2021|0 Comments

Product Insert: ViaStain Live Caspase 3/7 Detection

Download the Product Insert ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture for Celigo CS1-V0002-1 In real-time, measure apoptosis by detecting Caspase 3/7 in 2D live cell culture and 3D tumor spheroids. Nexcelom’s ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture kit is designed to detect caspase 3/7 activity in live cells. The ability to perform kinetic apoptosis assays allows researchers to continuously measure the caspase 3/7 activity within the cell population. This no wash assay has been shown to effectively work in both 2D and 3D cultures. The reagent (NucViewTM) consists of a nucleic acid-binding dye with a fluorescent [...]

By Polly Mitchell|2021-04-08T16:37:27+00:00April 8th, 2021|0 Comments

Product Insert: ViaStain No-Wash Annexin V-FITC Kit

Download the Product Insert ViaStain™ No-Wash Annexin V-FITC Kit (for Celigo) CSK-V0007-1 Apoptosis, or programmed cell death, is a natural process of cellular self-destruction. Apoptosis is a part of routine cell turnover and tissue homeostasis, prevalent in epithelial cells, erythrocytes, and other cell types genetically programmed to have a limited life span. It is also important in embryogenesis, maintenance of immune tolerance, and development of the nervous system. Apoptosis can be induced either by a stimulus, such as irradiation or toxic drugs, or by removal of a repressor agent. The cells disintegrate into membrane-bound particles that are then eliminated by [...]

By Polly Mitchell|2021-04-08T16:12:11+00:00April 6th, 2021|0 Comments

Product Insert: PI Apoptosis

Download the Product Insert Propidium iodide for the detection of dead and/or necrotic cells CS1-0116-1 Annexin V and propidium iodide are used to measure apoptosis and necrosis. Annexin V is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calciumdependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, PS translocates to the external leaflet. Fluorochrome-labeledAnnexin V can then be used to specifically target and identify the PS on the surface of apoptotic cells. Annexin V binding alone cannot differentiate between [...]

By Polly Mitchell|2021-04-06T19:18:17+00:00April 6th, 2021|0 Comments

Product Insert: Binding Buffer for Annexin V

Download the Product Insert Binding Buffer necessary for the Annexin V staining CS0-0115-1 Annexin V and propidium iodide are used to measure apoptosis and necrosis. Annexin V is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calciumdependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, PS translocates to the external leaflet. Fluorochrome-labeledAnnexin V can then be used to specifically target and identify the PS on the surface of apoptotic cells. Annexin V binding alone cannot differentiate between apoptotic and [...]

By Polly Mitchell|2021-04-06T19:05:49+00:00April 6th, 2021|0 Comments

Product Insert: Annexin V Staining Solution

Download the Product Insert Annexin V staining solution for the detection of apoptotic cells CS1-0114-1 Annexin V and propidium iodide are used to measure apoptosis and necrosis. Annexin V is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calciumdependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify the PS on the surface of apoptotic cells. Annexin V binding alone cannot differentiate [...]

By Polly Mitchell|2021-04-06T18:56:31+00:00April 6th, 2021|0 Comments

How to Upgrade Software

Please email support@nexcelom.com with your serial number to get instructions for upgrading.

By Polly Mitchell|2021-04-06T16:13:47+00:00April 6th, 2021|0 Comments

I need the Certificate of Analysis for my products(s)

If you need a Certificate of Analysis (COA) for your product(s), please send the catalog number and lot number to support@nexcelom.com and a member of our team will email the certificate to you. Not sure where to find these numbers? All reagent bottles have a label with both the catalog and lot number listed. The label will look similar to this example. Typical reagent label showing Cat# and Lot#

By Polly Mitchell|2022-04-04T14:24:45+00:00April 6th, 2021|0 Comments

About Polly Mitchell