Cellometer Bright Field and Fluorescent Cell Counters for Murine Primary Samples

Obtain fast, accurate, and consistent cell viability and concentration measurements of primary murine samples in < 30 seconds —

• Tissue debris, cell debris, and red-blood cell contamination can lead to inconsistent cell counts from sample to sample when performing manual counting
• Performing fluorescent-based analysis using Acridine Orange/ Propidium Iodide (AO/PI) allows for the identification and enumeration of only nucleated cells

Cell concentration and viability are two common measurements that are performed for great variety of primary murine samples: Mouse Tail Vein Bleeds, Splenocytes, Lymphocytes, BAL, Tissue Digests, Tumor Digests, and others.

Mouse Tail Vein Blood

• Fluorescent staining of mononuclear cells ONLY in mouse tail vein bleed sample.
• Red-blood cells seen in the bright-field are not stained and therefore are not counted.

Mouse Splenocytes

• Only nucleated cells are detected in each sample.
• Viability and Concentration measurements are based on the fluorescent detection of live (green) and dead (red) cells.

Mouse Bronchoalveolar Lavage Sample

• Only nucleated cells are stained with acridine orange.
• From multiple captured images the software automatically reports the cell concentration.

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Cellometer automatically quantifies cell viability and concentration for a variety of immunologically relevant samples such as: bone marrow, cord blood, splenocytes, lymphocytes, isolated mononuclear cells, tumor digests, murine samples, and others.