Trypan Blue Viability

Trypan Blue Viability: Viability is a measure of the metabolic state of a cell population which is indicative of the potential for growth. Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. Trypan blue dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging. Within 30 seconds, the Cellometer Auto T4 instrument counts both live cells and dead cells [...]

By |2021-06-15T20:53:08+00:00April 13th, 2012|Categories: About Cell-Based Assays, Cell Counting Leadership|0 Comments
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