2D Cell Migration Assay using 96-well Oris™ Platypus Plate
Validate wound healing migration assay with OrisTM Platypus plate on Celigo
Transwell migration using DAPI
Image and count the number of cells which have migrated through the membrane of a transwell insert using fluorescent detection of DAPI-stained cells.
PBMC-mediated cytotoxicity using calcein AM
Measure PBMC-mediated cytotoxicity by counting total live K562 cells over time in the presence and absence of IL2
NK92 cell-mediated ADCC using calcein AM
Measure antibody-dependent NK92 cell-mediated cytotoxicity using calcein AM-stained MDA-MB-231
3D Multicellular Tumor Spheroid (MCTS) Kinetic Propidium Iodide Assay
Kinetically monitor multicellular tumor spheroid (MCTS) growth & Propidium Iodide (PI) dead cell signal on U87MG Glioblastoma over mulitple days
3D multicellular tumor spheroid (MCTS) endpoint viability screening assay
Measure the effects of a panel of drugs on the viability of U87MG Glioblastoma MCTS using calcein AM and Propidium Iodide fluorescent staining
NK cell-mediated cytotoxicity using calcein AM
Measure NK cell-mediated cytotoxicity using calcein AM-stained K562 and IMR32 cells
Antibody-Dependent Drug Uptake Assay
Measure cellular drug uptake in adherent and suspension cell cultures of a fluorescently-labeled drug at varying antibody concentrations
GFP Transfection Efficiency Measurement
To analyze transfection efficiency over a 4 day period when treated with various amounts of a transfection compound.
Count and Measure Cell Infectivity of Microcarriers
In this experiment, we demonstrate the capability of Celigo to measure cell count on microcarrier beads and infected cells positively staining for AlexaFluor488-labeled antibody again the viral protein.