Cellometer Application News Archives | Page 2 of 5

A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision As part of the innate immune system, natural killer (NK) cells are the primary form of defense against tumor cells and assorted pathogens [1]. A minor subset of NK cells (CD56brightCD16-) influence immune regulation via the secretion of cytokines interferon-γ and TNF-α [2]. The major subset of NK cells (CD56dimCD16+), however, directly lyse their targets [3]. Consequently, understanding the cytolytic functions of NK cells are key to understanding NK cell biology and function in adoptive immunotherapy. Download our white [...]

By Leo Chan|2021-06-15T19:11:26+00:00March 2nd, 2016|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Cellometer Vision, Cytotoxicity, Naturan Killer Cells|0 Comments

Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

Over a time course analysis in standard cell culture, TB exclusion reports more viability in comparison to the fluorescence-based tools

By Leo Chan|2021-06-15T19:12:29+00:00February 24th, 2016|Categories: Cell Counting Leadership, Cellometer, Cellometer Application News, Instrument|Tags: Cellometer Vision, Morphological Analysis, Trypan Blue vs AOPI, Viability Staining|0 Comments

Cellometer assists in search to combat tumor radio-resistance

At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]

By Polly Mitchell|2021-06-15T19:18:34+00:00December 29th, 2015|Categories: Cellometer, Cellometer Application News, Instrument, Oncology / Tumor Microenvironment|Tags: Cell Counting, Cellometer, Trypan Blue|0 Comments

Using the Cellometer Vision Image Cytometer for Immunophenotyping

It's White Paper Wednesday! Read our featured white paper: Using the Cellometer Vision Image Cytometer for Immunophenotyping By developing fluorescent-based assays to immunophenotype cells, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this publication, we demonstrate an immunophenotyping assay to detect percentages of lymphocyte populations in the spleen and thumus via cell surface markers CD4, CD8, B220, and CD5 with Cellometer imaging cytometry as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry [...]

By Leo Chan|2021-06-15T19:47:54+00:00October 21st, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: B220, CD4, CD5, CD8, immunophenotyping, Surface marker, White Paper|0 Comments

Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Drug Effect on Cell Cycle Profile Using the Cellometer Vision Image Cytometer In this work, we demonstrate new applications of the Cellometer Vision for fluorescencebased cell population analysis as an alternative for flow cytometry. Cell cycle analysis was performed by inducing specific arrest in G0/G1, S, and G2/M phase of Jurkat cell population with aphidicolin, etoposide, and nocodazole, respectively [6-8]. The results were compared between the image-based and conventional flow cytometry methods. Download our white paper »

By Leo Chan|2021-06-15T19:48:44+00:00October 7th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Aphidicolin, Cell Cycle, Etoposide, Nocodazole, White Paper|0 Comments

Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Measuring Mitochondrial Membrane Potential with JC-1 Using the Cellometer Vision Image Cytometer By developing fluorescent-based assays to assess mitochondrial membrane potential, the Cellometer imaging cytometry can provide a quick, simple, and inexpensive alternative for biomedical research, which may be beneficial for smaller research laboratories and clinics. In this work, we demonstrate a mitochondrial membrane potential assay using Cellometer imaging cytometry and the JC-1 dye as an alternative to flow cytometry. The data obtained by Cellometer were compared to those from conventional flow cytometry methods. Download our white paper »

By Leo Chan|2021-06-15T19:48:56+00:00September 23rd, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Apoptosis, CCCP, Mitochondrial Potential, Necrosis, White Paper|0 Comments

Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision Cell concentration and viability of SVF preparations are usually determined by standard hemocytometer methods that are prone to considerable error since the operator must make judgments between actual cells versus "debris". To address that problem, we employed Cellometer image cytometry to perform both bright-field and fluorescence-based cell concentration and viability measurements [12]. Here, we validated this method for SVF analysis. First, the imaging parameters were optimized by measuring five adipose SVF samples. Next, the concentration and viability of three [...]

By Leo Chan|2021-06-15T19:49:43+00:00August 19th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Stromal Vascular Fraction (SVF), Viability, White Paper|0 Comments

Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Time-Course Monitoring of Primary Murine B1 and B2 Cell Proliferation using Cellometer Vision Image Cytometer Cell proliferation is an important assay for pharmaceutical and biomedical research to test the effects of a variety of treatments on cultured primary cells or cell lines [1, 2]. Previously, we have reported a rapid and accurate fluorescence-based cell population analysis method using a novel image-based cytometry system. The method is highly comparable to traditional flow cytometry using fewer cells [3-6]. Here we report the development of a novel method for the kinetic measurement of cell [...]

By Leo Chan|2021-06-15T19:50:01+00:00July 22nd, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: B Cells, B1, B2, Cell Proliferation, White Paper|0 Comments

Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Measuring Apoptosis and Necrosis Cell Populations by Heat and Compound Induction using the Cellometer Vision Recently, a new imaging cytometry system has been developed by Nexcelom Bioscience LLC (Lawrence, MA, USA) for fluorescence-based cell population analysis [1, 2]. Originally, the system has been used for automated cell concentration and viability measurement using disposable counting slides with both bright-field (BR) and fluorescent (FL) imaging methods [3]. The system has recently been demonstrated to perform fast and accurate cell population analysis comparable to the traditional flow cytometry method. Download our white paper »

By Leo Chan|2021-06-15T19:50:43+00:00June 24th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Apoptosis, Necrosis, White Paper|0 Comments

Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation Determining cell viability is a vital component in many biological experiments that range from standard cell culture to the investigation of pharmacological agents on tumor cells. One of the earliest and most common methods for measuring cell viability is the trypan blue (TB) exclusion assay [1, 2]. Over the last two decades, there have been various publications on comparing TB exclusion and fluorescence-based cellular viability assays [1, 3-5]. Previous results have shown that in a time-course measurement, TB exclusion [...]

By Leo Chan|2021-06-15T19:51:19+00:00May 20th, 2015|Categories: Cell Counting Leadership, Cellometer, Cellometer Application News, Instrument|Tags: Acridine Orange, Propidium Iodide, Trypan Blue, White Paper|0 Comments