Cellometer Application News Archives | Page 3 of 5

Measuring Physiological and Metabolic Characteristics of Yeast Health for Beer Fermentation Samples using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Measuring Physiological and Metabolic Characteristics of Yeast Health for Beer Fermentation Samples using the Cellometer Vision Characteristics such as the viability, vitality, glycogen, neutral lipid, and trehalose content of yeast samples must be measured to better understand changes in physiological and metabolic status during fermentation [4-9]. Monitoring changes of these parameters during fermentation can improve current production processes, and a subset of these parameters (glycogen, neutral lipid, and trehalose content) has been shown to play an important role in predicting yeast viability both during and after fermentation [10, 11]. Download our [...]

By Leo Chan|2021-06-15T19:52:03+00:00April 8th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Brewing, Viability, Vitality, White Paper, Yeast|0 Comments

Comparing Fluorescence-Based Viability Detection Method using the Cellometer Vision

It's White Paper Wednesday! Read our featured white paper: Comparing Fluorescence-Based Viability Detection Method using the Cellometer Vision In this work, Cellometer Vision was employed to demonstrate rapid fluorescence-based viability measurements and the comparison of various fluorescent staining methods. First, fluorescent nucleic acid stains that examine membrane integrity were tested and validated by comparing against the standard trypan blue exclusion method. Similarly, fluorescent enzymatic stains that examine metabolic activities were tested and validated against the standard trypan blue exclusion method. Third, nucleic acid and enzymatic stains were compared by measuring viabilities of Jurkat cells incubated at different temperatures of water [...]

By Leo Chan|2021-06-15T19:52:28+00:00March 25th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Fluorescent Viability Stains, White Paper|0 Comments

Detection of Multiplexed GFP Reporters in Primary Articular Chondrocyte Cultures Using Cellometer Vision Image Cytometer

It's White Paper Wednesday! Read our featured white paper: Detection of Multiplexed GFP Reporters in Primary Articular Chondrocyte Cultures Using Cellometer Vision Image Cytometer In this work, we have developed an image cytometry method for detecting and monitoring the cell expansion and differentiation of articular chondrocytes in primary culture. First, the feasibility of utilizing image cytometry for detection of fluorescent is shown by comparing measured fluorescent positive cell populations to flow cytometry. Next, articular chondrocyte cultures were established in multi-well plates from either single or Cyan/eGFP double reporter mouse lines and grown for 20 days to test the utility of the [...]

By Leo Chan|2021-06-15T20:34:28+00:00February 25th, 2015|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Articular Chondrocytes, CFP, GFP, RFP, White Paper|0 Comments

The Historical Development of the Hemacytometer

It's White Paper Wednesday! Read our featured white paper:The Historical Development of the Hemacytometer The hemacytometer has been an essential tool for hematologists, medical practitioners, and biologists for over a century. Depending on where it is being used, the word has multiple spellings such as hemacytometer, hemocytometer, haemacytometer, or haemocytometer, but for consistency purposes the word “hemacytometer” will be used in this review. The prefix “hema”, “hemo”, “haema”, or “haemo” means blood, while “cytometer” meant a device to measure cells. The device was initially used by medical practitioners to analyze patient blood samples, which was the initial spark that created [...]

By Leo Chan|2021-06-15T20:34:42+00:00February 11th, 2015|Categories: Cell Counting Leadership, Cellometer Application News|Tags: hemacytometer, Hemocytometer, History, White Paper|0 Comments

Cellometer Training Webinars available on YouTube!

Nexcelom hosts free, live monthly training webinars for our Cellometer instruments. Our webinars cover a variety of instruments, cell types and applications. We have taken our recorded webinars and added them to our YouTube channel! We've created a playlist entitled "Cellometer Training Webinars" and the list will continue to grow as we release more. This is a great resource for you and new lab team members to get additional training on your Cellometer instrument. We host new training webinars each month, so revisit our YouTube channel from time to time to see new content as it's produced and added.

By Polly Mitchell|2014-12-10T10:41:41+00:00December 10th, 2014|Categories: Cellometer Application News|Tags: Cellometer, Cellometer Auto 1000, Cellometer Mini, Cellometer Vision, Cellometer X2, training, webinar|0 Comments

Identifying and Resolving the Sources of Hemacytometer Counting Error through Automation

It’s White Paper Wednesday! This month’s featured white paper: Identifying and Resolving the Sources of Hemacytometer Counting Error through Automation The hemacytometer persists as the gold standard for laboratory cell counting. First utilized in 18th century France as a means to analyze patient blood samples, the hemacytometer has gone through a series of major developments over the past hundreds of years, creating a modern instrument that is more accurate and easier to use than its predecessors. The hemacytometer remains an integral part of all cell-based research, and yet sources of error inherent in its design and utilization persist. Those sources [...]

By Leo Chan|2021-06-15T20:36:59+00:00November 12th, 2014|Categories: Cell Counting Leadership, Cellometer Application News|Tags: Cell Counting, hemacytometer, Hemocytometer|0 Comments

Rapid Image Cytometry Method for Cell Counting and Viability Measurement for Cellular Therapy

Patients with cancers such as multiple myeloma, leukemia, lymphoma, and other metastatic cancers have tumor cells with unique immunological targets that when exploited, can lead to the complete destruction of the mass and a lasting remission from disease. Clinicians attack those targets using cell therapy, also known as “targeted immunotherapy”. Cell therapy is the programming of a patient’s own immune cells to target that patient’s tumor cells for destruction. One application of cell therapy, Adoptive Cell Transfer (ACT), employs select methods of genetic manipulation and propagation of those immune cells ex vivo so that the newly programmed cells can be [...]

By Leo Chan|2021-06-15T20:37:41+00:00October 13th, 2014|Categories: CAR T / Cell Therapy, Cellometer Application News, Instrument|Tags: Cell Counting, Cellular Therapy, image cytometry|0 Comments

Accurately Count PBMC and Measure Viability in Presence of Residual RBC

It’s White Paper Wednesday! This month’s featured white paper: Accurately Count PBMC and Measure Viability in Presence of Residual RBC In this work, we have developed an image cytometry method for detecting and monitoring the cell expansion and differentiation of articular chondrocytes in primary culture. First, the feasibility of utilizing image cytometry for detection of fluorescent is shown by comparing measured fluorescent positive cell populations to flow cytometry. Next, articular chondrocyte cultures were established in multi-well plates from either single or Cyan/eGFP double reporter mouse lines and grown for 20 days to test the utility of the fluorescence-based image cytometry system. [...]

By Leo Chan|2021-06-15T20:39:15+00:00October 1st, 2014|Categories: Cell Counting Leadership, Cellometer Application News|Tags: PBMCs, RBC Contamination, White Paper|0 Comments

Using Cellometer Vision and Cyto-ID Stain for Autophagy Detection in Living Cells

It's White Paper Wednesday! This month's featured white paper: Using Cellometer Vision and Cyto-ID Stain for Autophagy Detection in Living Cells Our understanding of autophagy has expanded tremendously in recent years, largely due to the identification of the many genes involved in the process, and the use of GFP-LC3 fusion proteins to visually monitor autophagosomes and autophagic activity both biochemically and microscopically [1, 2]. Recently, a novel fluorescent probe, Cyto-ID ® Green autophagy dye, has been developed to facilitate the investigation of the autophagic process [3-5]. In this study, a novel method was performed using the Cellometer image cytometry in combination with Cyto-ID Green autophagy dye for [...]

By Leo Chan|2021-06-15T20:40:28+00:00September 3rd, 2014|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Autophagosomes, Autophagy, Cyto-ID|0 Comments

Fast, accurate analysis of yeast, platelets, algae and other small cells

Acquire Fast, Accurate and Consistent Cell Viability and Concentration Measurements of Small Cells (2 microns or greater)! Obtaining consistent viability and concentration measurements for small cells like yeast, platelets or algae can be challenging. The Cellometers X1 and X2 can both perform bright-field and fluorescent counting of cells 2 microns and greater. X1 and X2 Cellometers are equipped with a 10X magnification lens. Both are capable of providing consistent concentration and viability measurements for cell samples that are 2 microns in size and greater. Both instruments are routinely used in laboratories around the world interested in studying small cells. See [...]

By Dmitry Kuksin|2021-06-15T20:40:42+00:00August 14th, 2014|Categories: Cellometer, Cellometer Application News, Instrument|Tags: Algae, Cellometer X2, Small Cells, Yeast|0 Comments