Immuno-oncology Archives | Page 2 of 2

Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines

South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]

By Polly Mitchell|2021-06-15T19:14:19+00:00February 9th, 2016|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|Tags: aspirin, Cancer Cell Lines, Cell Cycle, Cell Viability, Cellometer Auto T4, Trypan Blue|0 Comments

Cellometer Vision participates in study to evaluate effects of lead nitrate on human leukemia cells

At the NIH-Center for Environmental Health (Jackson, MS), a study was designed to analyze the impacts of lead nitrate, a component that has been greatly reduced in paint and ceramic products, and yet its exposure levels in humans remain a concern. Because industrial sources of lead persist in our environment, these researchers evaluated its effects on the DNA damage, cell cycle arrest, and induction of apoptosis in human leukemia cells (HL-60). The Cellometer Vision was used to measure live and necrotic cells with propidium iodide. After exposure to lead nitrate, the cells showed significant increases in necrotic death, DNA damage, [...]

By Polly Mitchell|2021-06-15T19:15:14+00:00January 28th, 2016|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|Tags: Apoptosis, Cell Cycle, human leukemia, lead paint, Propidium Iodide|0 Comments

Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer A panel of cytotoxic drugs, including doxorubicin and paclitaxel were used to study their effects on various breast cancer cell lines such as MDA-MD-436, MCF-7, SKBR3 and MDA-MB-231. Results show that the Colony Counting application can also be used to evaluate the clonogenicity and self-renewal of cancer stem/tumor-initiating cells by automatically analyzing mammosphere populations. The Colony Counting application on Celigo provides an efficient, reproducible and automated method for assessing the number, size, and morphology of cancer spheroids within [...]

By Leo Chan|2021-06-15T19:51:52+00:00April 22nd, 2015|Categories: Celigo, Celigo Application News, Immuno-oncology, Instrument|Tags: Breast Cancer Drugs, Mammospheres, White Paper|0 Comments

c-Myc inhibitor blocks proliferation of human ovarian cancer cells by inducing apoptosis and cell cycle arrest as reported by Cellometer Vision

Introduction The oncogene c-Myc is frequently overexpressed in ovarian carcinomas [1]. The small molecule c-Myc inhibitor, 10058-F4, was investigated here to evaluate its effects on ovarian cancer cell growth as well as its mechanism of action [2]. The inhibitor’s impact on apoptosis, cell cycle, cell colony formation, ROS generation, and cell viability were examined in Hey and SKOV3 cell lines. These findings suggest that small molecule c-Myc inhibitors may be a promising strategy for future ovarian cancer therapies. Materials and Methods Cell cycle analysis Hey and SKOV3 cell lines (both human ovarian cancer) were plated in 96-well plates and treated [...]

By Leo Chan|2021-06-15T20:35:02+00:00January 29th, 2015|Categories: Cellometer User Publications, Immuno-oncology|Tags: Apoptosis, Cell Cycle, Cellometer Vision, Ovarian Cancer|0 Comments

Evaluation of Nanoparticle-Based Therapy for Triple-Negative Breast Cancer using Cellometer Vision CBA

Introduction to Nanoparticle-Based Therapy for Triple-Negative Breast Cancer Tia Harmon and Dr. Ruben Gonzalez-Perez from the Morehouse School of Medicine and Emory University utilized the imaging and analysis capabilities of the Cellometer Vision to investigate leptin peptide receptor antagonist-conjugated nanoparticles in order to inhibit leptin signaling, a key pathway that promotes growth and survival of triple-negative breast cancer (TNBC) cells. It was hypothesized that these nanoparticles would first stop the expression of leptin’s downstream target Notch, and would thereby increase the effectiveness of standard chemotherapeutic compounds in limiting tumor cell survival [1-4]. Materials and Methods A human ER+ cell line (MCF-7) [...]

By Leo Chan|2021-06-15T20:36:25+00:00December 8th, 2014|Categories: Cellometer, Cellometer User Publications, Immuno-oncology, Instrument|0 Comments

Review: Unlicensed NK Cells Target Neuroblastoma Following Anti-GD2 Antibody Treatment

Review: Unlicensed NK Cells Target Neuroblastoma Following Anti-GD2 Antibody Treatment Author: Tarek, N., et al. J. Clin. Invest. 2012; 122(9): 3260-3270. doi:10.1172/JCI62749. Background: Neuroblastoma (NB) is the most common childhood extracranial solid tumor. Nearly two-thirds of diagnosed patients exhibit poor long-term survival despite aggressive treatment approaches. Treatment with monoclonal antibodies against the disialoganglioside surface antigen GD2 has resulted in lower recurrence and improved survival. The anti-GD2 mAb 3F8 utilizes antibody-dependent cell-mediated toxicity (ADCC) via myeloid and natural killer (NK) cells to kill the neuroblastoma. […]

By Polly Mitchell|2021-06-15T20:46:36+00:00October 19th, 2012|Categories: Immuno-oncology, Publication Review|0 Comments

Down-Regulation of Yes Associated Protein 1 Expression Reduces Cell Proliferation and Clonogenicity of Pancreatic Cancer Cells

BxPC-3, PANC-1, and HPDE6 cells were washed with DPBS, trypsinized, and then counted on Cellometer AutoT4 (Nexcelom Bioscience, Lawrence, MA) […]

By Polly Mitchell|2021-06-15T20:49:02+00:00July 27th, 2012|Categories: Cellometer User Publications, Immuno-oncology|0 Comments

Copper-transporting P-type adenosine triphosphatase (ATP7A) is associated with platinum-resistance in non-small cell lung cancer (NSCLC)

Human non-small cell lung cancer (NSCLC) cell lines included the parental cell line A549 and the cell subline A549/DDP. Pt levels were expressed as μM/1 × 106 cells, with the cell number determined by counting (Nexcelom Cellometer Auto T4 cell counter, USA) in parallel cultures. Experiments were performed in duplicate and the values expressed were the means ± SD of the three independent experiments. […]

By Polly Mitchell|2021-06-15T20:50:07+00:00July 27th, 2012|Categories: Cellometer User Publications, Immuno-oncology|0 Comments

Exploring Long-term Protection of Normal Human Fibroblasts and Epithelial Cells from Chemotherapy in Cell Culture

Exploring Long-term Protection of Normal Human Fibroblasts and Epithelial Cells from Chemotherapy in Cell Culture Authors: Pasha Apontes, Olga V. Leontieva, Zoya N. Demidenko, Fengzhi Li, Mikhail V. BlagosklonnyOncotarget. 2011 March 2(3): 222-233; PMCID: PMC3260824 Background: Microtubule-targeting agents or mitotic inhibitors are commonly used in chemotherapy for cancer. These agents kill proliferating, or cycling, cells by causing fatal mitotic arrest. Because many normal cells cycle very frequently (bone marrow cells, hair follicles, mucosal cells, endothelial cells), these normal cells are also affected by chemotherapy, leading to many side effects and reduced chemotherapy treatment. […]

By Polly Mitchell|2021-06-15T20:53:30+00:00March 28th, 2012|Categories: Immuno-oncology, Publication Review|1 Comment