Microbiology Archives | Nexcelom Bioscience

Cellometer Vision is used to characterize fungi suitable for pest management

Entomopathogenic fungi (ENPF)– those fungal species that use insect hosts to propagate – are of note in India due to their potential utility in pest management. The Indian Institute of Technology (New Delhi, India) obtained soil samples and isolated ENPF with the intention of studying how these types of fungi might be used to control the house fly, Musca domestica, an insect that causes many health problems in humans and animals in that country. The samples were then characterized molecularly and tested for pathogenicity against M. domestica. The Cellometer Vision was used to accurately maintain spore counts. Through this work, [...]

By Polly Mitchell|2021-06-15T19:13:29+00:00February 18th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Microbiology|Tags: Cellometer Vision, Entomopathogenic fungi, house fly, India, pest management, spore count|0 Comments

Cellometer helps to study microalgae metabolism for future industrial biotechnology applications

Researchers at the University of Manchester (UK) investigated the metabolism of microalgae Chlamydomonas reinhardtii, which holds great potential for future industrial biotechnology applications. This research uncovered that the typical lipid and starch accumulation by these microalgae during phosphorous starvation did not occur in the presence of mutant transcription factor PSR1. The Cellometer ensured accurate cell counts throughout experimentation. This work reports that PSR1 has significant control over the global metabolism of these cells, and that knowledge is of great importance to those who plan to develop microalgae to one-day produce pharmaceuticals, foods, and energy. Read the full publication here.

By Polly Mitchell|2021-06-15T19:13:45+00:00February 16th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Microbiology|Tags: Cell Counting, Cellometer, lipids, microalgae, starch|0 Comments

Cellometer T4 studies immune response to Delftia bacteria sometimes found in IV catheters

North Carolina State University investigated the immune response to the gram-negative bacteria Delftia spp., a water and soil species which has been identified in IV catheters. Delftia strain Cs1-4 and THP-1 cells (monocytes) were employed. Cellometer Auto T4 provided cell viability measurements with Trypan Blue to ensure a constant concentration of viable monocytes were present throughout experimentation. After Delftia stimulation of the monocytes, monocyte mortality rose, as did the production of TNF and pro-inflammatory proteins. This research confirms that Delftia does induce a pro-inflammatory reaction in monocytes and suggests that more research into the downstream effects of this bacteremia-associated strain [...]

By Polly Mitchell|2021-06-15T19:17:14+00:00January 12th, 2016|Categories: Cellometer, Cellometer User Publications, Instrument, Microbiology|Tags: Cell Viability, Cellometer Auto T4, immune system, Trypan Blue|0 Comments