Cold-chain Storage Optimization for Umbilical Cord Human Mesenchymal Stem Cells
Effective long-term cryopreservation of human mesenchymal stem cells is an essential component of regenerative medicine and tissue engineering applications.
Counting of Patient-Derived IDC Organoids
To image and count whole well populations of organoids in a rapid manner without having to take multiple, time consuming Z-stacked images
Cellometer Vision helps undergraduates explore immunophenotyping in mouse bone marrow stem cells
A collaboration between Merrimack College (North Andover, MA) and Nexcelom Bioscience LLC used a Cellometer Vision in an undergraduate immunology classroom to explore differentiation, activation, cell surface marker expression and cytokine production in mouse bone marrow stem cells. The Cellometer Vision allowed the students to visualize and analyze their cells for various surface markers before designing experiments to explore the activity of natural anti-inflammatory compounds on TNF-alpha production. Read the full publication here. More than 50% of Nexcelom employees hold advanced degrees - so it's no surprise that we love collaborations with other scientists and institutions. Many of our academic [...]
Cellometer Auto 2000 assists in developing new method to isolate and expand umbilical cord derived mesenchymal stromal cells
Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs [...]
Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale
Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]
Potential of human umbilical cord blood mesenchymal stem cells to heal damaged corneal endothelium
A portion of the GFP-labled or unlabeled UCB MSCs (umbilical cord blood mesenchymal stem / stromal cells) were incubated with trypan blue and counted using a Cellometer Auto T4 cell counter (Nexcelom Bioscience LLC, Lawrence,MA) to determine the number of viable cells. […]
Stem Cells
Stem Cells are undifferentiated cells within a tissue or organ that can develop into specialized cell types. Embryonic Stem Cells are derived from human embryos developed from eggs fertilized in vitro and donated for research. Though stem cell research has shifted away from embryonic stem cells for ethical and legal reasons, there are currently 21 approved stem cell lines that are monitored by the NIH Stem Cell Unit. Undifferentiated cells derived from cultured embryonic stem cells, or pluripotent cell lines, can be used to test new drugs and could potentially be used in cell-based therapy for a wide range of [...]
Direct Comparison of Different Stem Cell Types and Subpopulations Reveals Superior Paracrine Potency and Myocardial Repair Efficacy with Cardiosphere-Derived Cells
Direct Comparison of Different Stem Cell Types and Subpopulations Reveals Superior Paracrine Potency and Myocardial Repair Efficacy With Cardiosphere-Derived Cells Authors: Tao-Sheng Li, PhD., Ke Cheng, PhD., Konstantinos Malliaras, MD, et al. Journal of the American College of Cardiology 2012; 59(10): 942-953; doi:10.1016/j.jacc.2011.11.029. Background: Many types of stem cells have been used clinically in attempts to treat damaged hearts. The discovery of cardiac stem cells within the heart and the development of a technique for expansion of cardiosphere-derived cells (CDCs) has given researchers a new option in the use of stem cells for regenerative therapy. Purpose: To evaluate the success [...]