Kansas State University scientists developed a new method by which to isolate and expand umbilical cord derived mesenchymal stromal cells (UCMSCs). Rather than dissecting blood vessels, this method uses a dissociator followed by enzymatic digestion. This reduces contamination and hands-on time and produces ten times more cells per cm of tissue than other processes. The Cellometer Auto 2000 and AO/PI were used to count live cells and record cell size. The scientists validated the cells obtained from this method, demonstrating the cells’ expression of the standard surface markers CD90, CD105, CD73, CD44, as well as their pluripotent differentiation potential. UCMSCs have significant immunomodulatory potential, making them the focus of many clinical studies each year. It is hoped that improved collection methods, such as this one, will only expand the use of this therapeutically important cells.
The Cellometer Auto 2000 is a versatile all-in-one, stand alone instrument that provides dual fluorescent imaging capabilities, optimized for primary cell samples. Learn more about how the Auto 2000 could benefit your research!