Breast Cancer Drugs Archives | Nexcelom Bioscience

Researchers in Turkey use Cellometer for Trypan Blue Viability

Here’s another great example of how the Cellometer instruments are used to test viability of cells prior to plating. Standard therapy zoledronic acid (ZA) is typically used for metastatic bone disease in breast cancer patients, but it’s mechanism of action is not clear. Here, Turkish scientists investigated zoledronic acid with and without serine-threonine inhibitors in human MCF-7 and MDA-MB-231 breast cancer cells. Cellometer was used in combination with tryan blue to assess cell viability prior to plating. After plating, the cells were treated with various combinations of therapeutic agents. ZA with either one of the serine-threonine inhibitors examined proved more [...]

Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer A panel of cytotoxic drugs, including doxorubicin and paclitaxel were used to study their effects on various breast cancer cell lines such as MDA-MD-436, MCF-7, SKBR3 and MDA-MB-231. Results show that the Colony Counting application can also be used to evaluate the clonogenicity and self-renewal of cancer stem/tumor-initiating cells by automatically analyzing mammosphere populations. The Colony Counting application on Celigo provides an efficient, reproducible and automated method for assessing the number, size, and morphology of cancer spheroids within [...]