Cellometer Vision Archives | Page 2 of 2

Cellometer Vision provides new method for measuring cytotoxic potential in Natural Killer Cells

The MD Anderson Cancer Center worked in collaboration with Nexcelom to create a new method by which to measure the cytotoxic potential of natural killer (NK) cells. The traditional non-radioactive method, calcein release, is subject to variation, with differing dynamic ranges depending on tumor type. The Cellometer Vision and calcein, in combination with K562, 721.221, and Jurkat cells, were used to develop a novel, image cytometry-based assay to ascertain NK cytotoxicity. Using fluorescent intensity gating to ignore dimmer cells and apoptotic bodies, image cytometry provided a way to measure tumor cell lysis in a specific manner with the same experimental [...]

By Polly Mitchell|2021-06-15T19:46:24+00:00November 12th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cellometer Vision, Image Cytometer, image cytometry, jurkat cells, natural killer cells|0 Comments

Rowan Univ Researchers using Cellometer Vision for PI Viability, Caspase-3 activation and ROS

The Cellometer instruments have a wide range of functionality – this publication demonstrates how an instrument such as the Cellometer Vision can be used for more than just viability and can perform many cell-based assays. Fructose differs from glucose in that it is not regulated by a negative feedback loop and can thereby negatively impact intracellular energy stores. As the incidence of diabetes and obesity have risen in this country, so has the intake of fructose, making fructose’s effects on pancreatic cells important to understand. Here, researchers at Rowan University revealed that fructose sensitizes pancreatic beta cells to TNF-a-induced toxicity. [...]

By Polly Mitchell|2021-06-15T19:47:19+00:00November 4th, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Cell Viability, cell-based assays, Cellometer Vision|0 Comments

c-Myc inhibitor blocks proliferation of human ovarian cancer cells by inducing apoptosis and cell cycle arrest as reported by Cellometer Vision

Introduction The oncogene c-Myc is frequently overexpressed in ovarian carcinomas [1]. The small molecule c-Myc inhibitor, 10058-F4, was investigated here to evaluate its effects on ovarian cancer cell growth as well as its mechanism of action [2]. The inhibitor’s impact on apoptosis, cell cycle, cell colony formation, ROS generation, and cell viability were examined in Hey and SKOV3 cell lines. These findings suggest that small molecule c-Myc inhibitors may be a promising strategy for future ovarian cancer therapies. Materials and Methods Cell cycle analysis Hey and SKOV3 cell lines (both human ovarian cancer) were plated in 96-well plates and treated [...]

By Leo Chan|2021-06-15T20:35:02+00:00January 29th, 2015|Categories: Cellometer User Publications, Immuno-oncology|Tags: Apoptosis, Cell Cycle, Cellometer Vision, Ovarian Cancer|0 Comments

Cellometer Vision uncovers new mechanisms by which Type I interferons direct autophagy in vitro

Introduction A diverse family of cytokines, Type I interferons (IFNs), is a group of proteins responsible for antiviral, antiproliferative, and immunomodulatory functions [1, 2]. Treating cells with Type I IFNs induces autophagy, a cellular recycling process that is considered a key cell survival strategy [3]. It is well known that Type I IFNs work through the JAK-STAT pathway, but recent evidence suggests that the MAP kinase pathway can affect the expression of IFN-regulated genes [4, 5]. Researchers demonstrated that IFN-induced autophagy affects cell cycle as well as cellular proliferation in a variety of cell lines. Because autophagy plays a major [...]

By Leo Chan|2021-06-15T20:35:18+00:00January 22nd, 2015|Categories: Cellometer, Cellometer User Publications, Instrument|Tags: Autophagosomes, Autophagy, Cell Cycle, Cellometer Vision, Cyto-ID, Proliferation|0 Comments

Cellometer Training Webinars available on YouTube!

Nexcelom hosts free, live monthly training webinars for our Cellometer instruments. Our webinars cover a variety of instruments, cell types and applications. We have taken our recorded webinars and added them to our YouTube channel! We've created a playlist entitled "Cellometer Training Webinars" and the list will continue to grow as we release more. This is a great resource for you and new lab team members to get additional training on your Cellometer instrument. We host new training webinars each month, so revisit our YouTube channel from time to time to see new content as it's produced and added.

By Polly Mitchell|2014-12-10T10:41:41+00:00December 10th, 2014|Categories: Cellometer Application News|Tags: Cellometer, Cellometer Auto 1000, Cellometer Mini, Cellometer Vision, Cellometer X2, training, webinar|0 Comments