LNCaP and PC-3 cells
Cells were gently trypsinized and staining with trypan blue dye. The viable cells were counted using cell counting chamber every 24 h for 7 days.
48 h after transfection, cells were treated with Triptolide for another 48 h. After stained with trypan blue, viable cells were counted using cellometer Auto T4 automated cell counter.
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