LNCaP and PC-3 cells

Cells were gently trypsinized and staining with trypan blue dye. The viable cells were counted using cell counting chamber every 24 h for 7 days.

48 h after transfection, cells were treated with Triptolide for another 48 h. After stained with trypan blue, viable cells were counted using cellometer Auto T4 automated cell counter.