Trypan Blue vs. Acridine Orange/Propidium Iodide to measure cell viability

Do you have red-blood cells, cell debris, or tissue debris in your samples?

When your samples contain unwanted particles use:
Acridine Orange/ Propidium Iodide (AO/PI) viability assay to accurately measure the concentration and viability of your samples!

AO/PI Protocol

1. Obtain Nexcelom AO/PI solution: Cat # CS2-0106-5ML
2. Stain cell sample at 1:1 with AO/PI solution
3. Load counting chamber slide with 20 µl of cells and analyze
4. Report Accurate and Consistent concentration and viability Results

Do you have clean cell samples without RBCs, or other cell debris?

When your samples are free from unwanted particles,
Use Trypan Blue exclusion assay to accurately measure the concentration and viability of your samples!

Trypan Blue Protocol

1. Dilute the stock (0.4 %) with PBS to 0.2 %
2. Filter the trypan blue with 0.2 micron filter
3. Mix the cell suspension at 1:1 with 0.2 % trypan blue
4. Load the counting chamber slide with 20 µl of cells into the Cellometer and analyze
5. Report Accurate and Consistent concentration and viability Results