Last week we presented an in-depth look at Normalizing Seahorse™ XF Data with Label-Free Cell Counting. Today, we are taking a step back to give you an at-a-glance look at the Seahorse™ normalization assay workflow.

This short video describes the assay principle and illustrates the workflow from cell seeding to normalized data. Celigo is used to directly count adherent or suspension cells in a Seahorse™ XF plate for normalizing cell metabolic data. Celigo’s concurrent imaging and analysis takes less than 5 minutes.

For more information, download the application note here or read more here.

*Seahorse™ XF Analyzer is a product of Agilent – https://www.agilent.com/en/products/cell-analysis/seahorse-analyzers