Ever lose count when manually counting?

https://youtu.be/4xUYjVIgOdQ If you're using a microscope and hemacytometer to count your cells and determine viability, you might have felt like Sheldon, when he vacations in Amy's lab and, among other things, helps out by counting the bacteria spores.  Everything's going well until Amy returns to the lab, distracts Sheldon, and then he has to start over with his count. Fortunately for you, you don't have to be like Sheldon if you have an automated cell counter. We have bright field counters for cultured cells and Trypan Blue exclusion assays. We have dual fluorescent cell counters for messy, primary samples [...]

By |2017-01-19T14:21:50+00:00January 19th, 2017|Categories: About Cells|Tags: , , , |0 Comments

Having a tough time counting your PBMCs?

Cellometer Auto 2000 cell counter can detect live and dead nucleated cells while excluding debris, red blood cells, platelets in your sample.

Accurate Measurement of Peripheral Blood Mononuclear Cell Concentration using Image Cytometry to Eliminate RBC-Induced Counting Errors

Peripheral blood mononuclear cells (PBMCs) have been widely researched in the fields of immunology, infectious disease, oncology, transplantation, hematological malignancy, and vaccine development. Specifically, in immunology research, PBMCs have been utilized to monitor concentration, viability, proliferation, and cytokine production from immune cells, which are critical for both clinical trials and biomedical research. […]

By |2013-01-23T16:08:44+00:00January 23rd, 2013|Categories: About Cells|0 Comments

Natural Killer (NK) Cells

Natural Killer (NK) Cells are large granular lymphocytes that belong to the innate immune system and make up approximately 10% of circulating lymphocytes. Unlike T cells, NK cells do not express CD3. NK cells are critical for protection from life-threatening infections and are important mediators of antitumor immunity. Rare reports of complete NK-cell deficiencies in humans have resulted in fatal infection during childhood. Uncontrolled or inappropriate NK cell response can lead to pathological conditions such as allograft rejection, graft vs. host disease, diabetes, aplastic anemia, and various autoimmune and neurological diseases. […]

By |2012-10-12T11:52:36+00:00October 12th, 2012|Categories: About Cells|0 Comments

Adipocytes

¬†Adipocytes are the primary cells found in adipose (fat) tissue. Adipocytes store energy for the body in the form of triacylglycerol and play a critical role in lipid metabolism and energy regulation. Adipocytes have also been found to express a wide range of cytokines, chemokines, receptors, and cell adhesion molecules capable of inducing inflammation. Though it was once believed that mature adipocytes do not proliferate, a small degree of proliferation has been observed in mature adipocytes in culture. It is likely that overall adipocyte cell number is maintained over time by highly-controlled apoptosis and proliferation. […]

By |2012-08-16T13:06:20+00:00August 16th, 2012|Categories: About Cells|0 Comments

Stem Cells

Stem Cells are undifferentiated cells within a tissue or organ that can develop into specialized cell types. Embryonic Stem Cells are derived from human embryos developed from eggs fertilized in vitro and donated for research. Though stem cell research has shifted away from embryonic stem cells for ethical and legal reasons, there are currently 21 approved stem cell lines that are monitored by the NIH Stem Cell Unit. Undifferentiated cells derived from cultured embryonic stem cells, or pluripotent cell lines, can be used to test new drugs and could potentially be used in cell-based therapy for a wide range of [...]

By |2012-06-28T12:38:59+00:00June 28th, 2012|Categories: About Cells|0 Comments

Jurkat Cells

Jurkat is an acute T cell leukemia cell line originally derived from the peripheral blood of a 14-year-old boy. The original Jurkat clone was designated JM*. The E6-1 clone, derived from JM, is now the Jurkat cell line used most frequently by researchers, but many other clones or derivatives are available. Jurkat cells are widely used for IL-2 production and in the study of acute T cell leukemia, T cell signaling, and the effectiveness of potential anti-cancer compounds. Jurkat cells were used to establish the Nexcelom Apoptosis (Annexin V-FITC / PI) and PI Cell Cycle protocols for the Vision CBA [...]

By |2012-06-13T16:50:54+00:00June 13th, 2012|Categories: About Cells|0 Comments

Hepatocytes

Hepatocytes are the predominant cell in the liver, comprising about 60% of liver cells and 80% of liver mass. One of the main hepatocyte functions is the production of bile. Hepatocytes are also responsible for the regulation of metabolism, detoxification, and the manufacture of important circulating proteins, making them a critical component of studies involving liver disease, drug metabolism, and toxicology. […]

By |2012-03-30T12:57:16+00:00March 30th, 2012|Categories: About Cells|0 Comments