Poster: High-throughput immune complex analysis method for CAR T cell-mediated cytotoxicity using the Celigo Image Cytometer

Download our Poster Abstract: Cancer immunotherapy has been gaining momentum in the field of cancer research. Specifically, Chimeric Antigen Receptor (CAR) T cell technology have introduced new methods to combat cancer. Direct cell-mediated cytotoxicity assays are required to assess the killing capability of the engineered CAR T cells. Traditionally, these assays are conducted by measuring the amount of released Chromium, calcein AM, or LDH molecules after the target cancer cells are killed with CAR T cells. These methods require a large amount of target cells which may not be ideal when working with donor primary samples. Additionally, they cannot [...]

By | 2018-06-21T14:01:23+00:00 April 5th, 2018|Categories: Celigo Application News, Latest News|Tags: , , , |0 Comments

High throughput, high-resolution image acquisition for DNA damage detection

Celigo playing an important role in high throughput, high-resolution image acquisition for DNA damage detection. The Celigo S captures 16 images of each well that are then stitched into a single representation Open access article: Next generation high throughput DNA damage detection platform for genotoxic compound screening

By | 2018-06-15T15:35:51+00:00 March 2nd, 2018|Categories: Celigo Application News, Latest News|Tags: , , , |0 Comments

Benefits of direct high-throughput cell counting in immuno-oncology research

Antibody-directed and drug therapies have been on the forefront of immuno-oncology research in the past 15 years. Since the FDA introduced the Breakthrough Therapy Designation in 2016 for novel promising drug therapies, the number of applicants and approved candidates has increased dramatically. As more and more researchers are looking at drug effects in cancer cells and moving forward in drug discovery, they face many challenges in their day to do work. Performing cell cytotoxicity assays for multiple cell lines with single and combination drug therapies is standard practice in immuno-oncology. While many researches use end-point assays such as MTT, XTT, WST, or CellTiter-Glo, others prefer the manual [...]

Celigo 5 Channel system officially released

Lawrence, Massachusetts – September, 2016 - Nexcelom Bioscience, a leading provider of cell counting and analysis products for biomedical research and the biopharma industry, announced it has released the addition of a 5th channel available as an option on the Celigo image cytometer. The Celigo is a bench-top image cytometry system providing whole-well imaging and quantitative data, through image analysis for demanding cell-based analytical applications. Previous configurations allowed bright field only imaging, or bright field imaging plus 3 fluorescent channels. This new option of bright field plus 4 fluorescent (red, green, blue and far-red) channels will allow researchers more capabilities [...]

By | 2016-09-08T16:30:19+00:00 September 8th, 2016|Categories: Celigo Application News|Tags: |0 Comments

Celigo Advances studies in T Cell Therapy as a pediatric CHOP patient defeats her recurrent leukemia with immunotherapy

Emily Whitehead, cancer survivor, holding Nexcelom cell counting sheep. Thanks to the lifesaving T cell therapy clinical trial at Children’s Hospital of Philadelphia, Emily Whitehead is now two years cancer free. Diagnosed with acute lymphoblastic leukemia (ALL) right after her 5th birthday, doctors discovered that Emily’s leukemia was particularly resistant to chemotherapy, as are roughly 15% of the total number of ALL cases. After two recurrences of the disease, Emily’s parents enrolled her in a clinical trial for CTL019, an experimental therapeutic using a patient’s own reprogrammed T cells to eliminate the cancer cells. Now 9 years old, [...]

Live Webinar! Join us for “3D Tumor Spheroids Based Functional Assays”

Recent publications have suggested that using 3D tumor spheroids is a more predictive model for preclinical research. Nexcelom Bioscience has developed a standardized microplate method for rapid generation, imaging and analysis of 3D tumor spheroids using the Celigo image cytometer. 40 cancer cell lines' ability to form spheroids, optimal seeding densities and culture conditions have been established. Protocols measuring tumor growth, viability, migration and invasion have been utilized by many researchers for routine preclinical drug studies. If you are interested to learn more about how your research might benefit from working with 3D tumor spheroids, this is a great place to start! [...]

By | 2015-10-13T10:40:17+00:00 October 13th, 2015|Categories: Celigo Application News|Tags: , , |0 Comments

Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Increasing Efficiency in Cell Line Development using the Celigo Imaging Cytometer In this study, using the CHO cell line, the Celígo Imaging Cytometer was used to monitor transfection efficiency, identify passaging times, optimize media, identify single cells and track clone outgrowth, following previous studies detailing Celígo's performance in tracking cell lineage and confluence [3, 4]. After transfection, cells were allowed to stabilize, then imaged in bright field and fluorescence to monitor and evaluate transfection efficiencies. As wells grew asynchronously, bright field imaging was used to track which wells needed passaging. As [...]

By | 2015-09-09T10:30:42+00:00 September 9th, 2015|Categories: Celigo Application News|Tags: , |0 Comments

A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: A Rapid and Label-Free In Situ Assay Method for Cell Proliferation and Drug Toxicity using Celigo Imaging Cytometer In this study, Celigo was used to screen a compound library for effects on cell proliferation in adherent and non-adherent cell lines. Human lung carcinoma (A549) and promyelocytic leukemia (HL-60) cells were treated with a panel of compounds to inhibit proliferation. Finally, the Celigo system used image-based analysis to measure changes in cell morphology upon compound treatment. These data indicate that certain anti-proliferative compounds can have secondary effects on cell health or physiology, [...]

By | 2015-08-05T10:30:08+00:00 August 5th, 2015|Categories: Celigo Application News|Tags: , , , |0 Comments

3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: 3D Tumor Spheroid Analysis Method for HTS Drug Discovery using Celigo Imaging Cytometer U87MG cells were used to create tumorspheres in 384-well plates that were subsequently analyzed by imaging. The data illustrate that reproducible 3D spheroids can be formed in 384-well plates. Fluorescent viability studies were carried out with the imager using pixel intensity analysis. Moreover, the assay was validated for drug screen using various drug compounds that have shown anti-proliferative effects. Together, these data demonstrate that the tumorsphere formation assay can be developed, validated and used for high-throughput anti-cancer compound [...]

By | 2018-06-20T20:31:14+00:00 July 8th, 2015|Categories: Celigo Application News|Tags: , , , |0 Comments

Non-Disruptive Quantification of 2° Reprogrammed iPS Colonies Using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Non-Disruptive Quantification of 2° Reprogrammed iPS Colonies Using Celigo Imaging Cytometer Nexcelom's Celigo S imaging cytometer has been applied to provide automated, rapid assessment of iPSC reprogramming. Using f-theta optics, Celigo provides high quality, whole well images using bright field and/or fluorescent illumination. Automated segmentation and analysis provides quantitative output of iPSC reprogramming based on mOrange and GFP fluorescent colony detection. Download our white paper »