Validate wound healing migration assay with OrisTM Platypus plate on Celigo
Image and count the number of cells which have migrated through the membrane of a transwell insert using fluorescent detection of DAPI-stained cells.
Measure PBMC-mediated cytotoxicity by counting total live K562 cells over time in the presence and absence of IL2
Measure antibody-dependent NK92 cell-mediated cytotoxicity using calcein AM-stained MDA-MB-231
Kinetically monitor multicellular tumor spheroid (MCTS) growth & Propidium Iodide (PI) dead cell signal on U87MG Glioblastoma over mulitple days
Measure the effects of a panel of drugs on the viability of U87MG Glioblastoma MCTS using calcein AM and Propidium Iodide fluorescent staining
Measure NK cell-mediated cytotoxicity using calcein AM-stained K562 and IMR32 cells
Measure cellular drug uptake in adherent and suspension cell cultures of a fluorescently-labeled drug at varying antibody concentrations
To analyze transfection efficiency over a 4 day period when treated with various amounts of a transfection compound.
In this experiment, we demonstrate the capability of Celigo to measure cell count on microcarrier beads and infected cells positively staining for AlexaFluor488-labeled antibody again the viral protein.