Everyone I meet that performs primary cell counting wants to optimize the amount of time they spend doing that task. They also agree that 30 seconds per count sounds pretty good.
Nexcelom Automated Cell Counters: Accurate, Reproducible Cell Counts for the Entire Cell Therapy Workflow
Cellometer automated cell counters have been facilitating CAR T manufacturing with accurate cell counting and analysis since 2010.
If you use Brettanomyces in your brewery, you know that counting brett cells is complicated. Leo Chan joins MBAA to discuss the development of a method to automate cell counting of Brettanomyces. Check out the podcast here: https://goo.gl/pGT3vs
A study recently published in the Journal of Insect Behavior looks at the mating system of honey bees. Cellometer Vision CBA was used to count and determine the viability of collected honey bee sperm. Read more
Congratulation to Nexcelom's Dr. Leo Chan for his Inge Russell Best Paper Award form @MasterBrewers . Dr. Chan's paper, "Measuring Lager and Ale Yeast Viability and Vitality Using Fluorescence-Based Image Cytometry" examines the use of image cytometry for multichannel fluorescent assessment of yeast viability and vitality during the brewing process.
Counting cells with a hemacytometer is a long, dull process on its own – but if you’re trying to count cells that are clumped together in your sample, it can become a real headache! Clumpy samples make it hard to discern the individual cells from one another, causing a high risk of human error and skewed cell counts. Your results can be extremely difficult to reproduce, as the cell count would likely vary from person to person counting the same clumpy sample, causing a lack of confidence in your data. Luckily, we have a solution. The Cellometer Auto T4 automated [...]
Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision
It's White Paper Wednesday! Read our featured white paper: Novel Fluorescent Viability and Vitality Detection Method for Ale and Lager Yeast Fermentation using Cellometer Vision Automated cell counting methods can monitor yeast concentration and viability throughout fermentation to ascertain cell health and the amount of yeast to be pitched or repitched, all of which contributes to the quality and flavor consistency of the final product [1,2,3]. Analyzing physiological and metabolic characteristics of the yeast cells permits operators to efficiently monitor yeast viability and vitality for quality control purposes, which impacts long-term storage and other physiological stresses. Download our white [...]
It's White Paper Wednesday! Read our featured white paper: A Novel NK Cell-Mediated Cytotoxicity Detection Method Using the Cellometer Vision As part of the innate immune system, natural killer (NK) cells are the primary form of defense against tumor cells and assorted pathogens . A minor subset of NK cells (CD56brightCD16-) influence immune regulation via the secretion of cytokines interferon-γ and TNF-α . The major subset of NK cells (CD56dimCD16+), however, directly lyse their targets . Consequently, understanding the cytolytic functions of NK cells are key to understanding NK cell biology and function in adoptive immunotherapy. Download our white [...]
It's White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation These studies reveal that over a time course analysis in standard cell culture, TB exclusion reports more viability in comparison to the fluorescence-based tools, although the studies did not speculate as to the reasoning behind the differences noted (Jones and Senft 1985; Mascotti et al. 2000). In this work, we perform a head-to-head comparison between cell viability as determined by TB and that determined by AO/PI using an automated image-based cytometer, the Cellometer. Image-based cytometry allowed us to examine the [...]
At the University of Luebek (Germany), scientists investigated how a mechanism of tumor hypoxia - Aryl hydrocarbon receptor nuclear translocator (ARNT), a transcription factor also known as hypoxia-inducible factor (HIF)-1β - increases a tumor’s resistance to radiation therapy. ARNT expression was knocked out with siRNA or overexpressed using a plasmid vector in a variety of human tumor cell lines such as Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl before exposure to X-irradiation. The Cellometer and Trypan Blue were used to establish cell counts. Researchers found that a reduction of ARNT expression made all cell lines more susceptible to X-irradiation, whereas [...]