/Cellometer User Publications

Cellometer Auto 2000 participates in new method for manufacturing human umbilical cord mesenchymal stromal cells on an industrial scale

Human mesenchymal stromal cells (hMSCs) hold enormous promise for regenerative medicine. Kansas State University researchers investigated the growth parameters necessary to propagate hMSCs on a larger scale, moving from static cultures to the scope of stirred bioreactor tanks. Now that this group has optimized a new method by which to isolate and expand these hMSCs (please see companion paper by this group), the next challenge was producing enough cells to satisfy future clinical needs. The researchers investigated the growth kinetics and metabolic needs of these cells as the propagation scope increased. The Cellometer Auto 2000 recorded cell viability, size, and [...]

Cellometer helps with culturing optimization necessary for future cell-based therapies

EMD Millipore Corporation scientists (Bedford, MA) investigated the various media and microcarrier components necessary to optimize the large-scale manufacture of mesenchymal stem cell cultures that will be required for future cell-based therapies. To ensure the quality and consistency necessary to grow these cultures within tank bioreactors, the media and matrix components for that scale of undertaking must be optimized. Adipose-derived mesenchymal stem cells (MSCs) were grown on various microcarriers in Petri dishes, spinner flasks, and bioreactors. Human bone marrow-derived MSCs were used to assess various media options in T flasks and spinner flasks. The Cellometer was used to maintain accurate [...]

Cellometer T4 studies immune response to Delftia bacteria sometimes found in IV catheters

North Carolina State University investigated the immune response to the gram-negative bacteria Delftia spp., a water and soil species which has been identified in IV catheters. Delftia strain Cs1-4 and THP-1 cells (monocytes) were employed. Cellometer Auto T4 provided cell viability measurements with Trypan Blue to ensure a constant concentration of viable monocytes were present throughout experimentation. After Delftia stimulation of the monocytes, monocyte mortality rose, as did the production of TNF and pro-inflammatory proteins. This research confirms that Delftia does induce a pro-inflammatory reaction in monocytes and suggests that more research into the downstream effects of this bacteremia-associated strain [...]

Cellometer T4 work in Adoptive Cell Transfer examines gender differences in pathology after stroke

Oregon Health & Science University researchers continued their studies into the gender-related differences in peripheral immune system response after stroke that produce greater downstream damage in males than females, and what role the spleen may play in those differences. The goal of this work was to uncover which subset of immune system cells may contribute to those pathogenic effects after a stroke and how they vary by gender. Using primary leukocytes from transgenic mice and adoptive T-cell transfer, scientists injected specific cell types into splenectomized male and female mice 24 hours after stroke was induced. The Cellometer Auto T4 maintained [...]

Cellometer Auto T4 evaluates the cytotoxic potential of mouth rinses

How safe is your mouthwash? Researchers in Germany worked to answer that question. University of Leipzig (Germany) researchers investigated the cytotoxic effects of different antimicrobial mouth rinses (MRs) on gingival fibroblasts and epithelial cells. Although effective at killing infectious agents, most MRs have some cytotoxic impact on host tissue, which could delay the healing process the rinses are meant to enable. Human primary gingiva fibroblasts and human primary nasal epithelial cells were exposed to various MRs (Octenidol (OCT), Chlorhexidine 0.2% (CHX), Listerine (LIS), Meridol (MER), Betaisodona (BET), and control) for varying lengths of time. The Cellometer Auto T4 analyzed cell [...]

Cellometer used in study to examine secondary effects of MAO inhibitors

In the “catecholaldehyde hypothesis” of Parkinson’s disease neurodegeneration, cytoplasmic dopamine (DA) is converted to 3,4-dihydroxyphenylacetaldehyde (DOPAL) by the enzyme monoamine oxidase-A (MAO-A). DOPAL is a toxic substance, generating free radicals and inhibiting mitochondrial function. Scientists at NIH wanted to compare the abilities of currently available MAO-A and MAO-B inhibiting drugs at decreasing endogenous DOPAL levels. Using PC12 cells, six drugs were tested for their effects on DOPAL: MAO-A inhibitor clorygyline, three reversible MAO-A inhibitors, and the MAO-B inhibitors selegiline and rasagiline. The Cellometer was used to count the cells throughout experimentation. Clorgyline, rasagiline, and selegiline decreased DOPAL levels in both [...]

By |2015-12-22T11:52:59+00:00December 22nd, 2015|Categories: Cellometer User Publications|Tags: , , , |0 Comments

Cellometer assists in characterization of adipocytic extracellular vesticles

Adipocytes have been identified as a source of extracellular vesicles (EVs), which have, in turn, been connected to obesity-mediated cardiovascular disease. But these vesicles have not been well studied, particularly pre- and post-adipogenesis. Researchers at Cardiff University (UK) set out to characterize these EVs to assess their structure, composition, and function. 3T3-L1 cells were used to harvest EVs which were then characterized via electron microscopy, flow cytometry, and 2D chromatography. The Cellometer and Trypan Blue established cell counts throughout experimentation. Before adipogenesis, EVs display “classic” morphology, but the number of EVs rise just before adipogenesis and are enriched with signaling [...]

By |2015-12-17T11:50:09+00:00December 17th, 2015|Categories: Cellometer User Publications|Tags: , , , , |0 Comments

Cellometer participates in study to ascertain rotenone mechanism of action

A common hypothesis concerning the pathogenesis of Parkinson’s neurodegeneration suggests that cytoplasmic dopamine (DA) is converted to the auto-toxic 3,4-dihydroxyphenylacetaldehyde (DOPAL), which is neutralized by aldehyde dehydrogenase (ALDH). NIH researchers studied whether rotenone, a substance typically used to create in vitro models of Parkinson neurodegeneration, inhibits ALDH function. PC12 cells were exposed to various concentrations of rotenone in the presence or absence of F-dopamine. Catecholamine metabolism was then measured. The Cellometer maintained cell counts throughout experimentation. Rotenone increased DOPAL while decreasing dopamine levels in cell culture, but in a test tube, the DOPAL to DOPAC conversion by ALDH was not [...]

By |2015-12-15T10:00:57+00:00December 15th, 2015|Categories: Cellometer User Publications|Tags: , , |0 Comments

Cellometer helps to elucidate underlying mechanism of hypomyelination

Vanderbilt University researchers sought to evaluate the role of mTor signaling during myelination. Other work suggests that myelin abnormalities may be distinct from cortical lesions, and these scientists investigated the underlying mechanisms of hypomyelination to support or refute the other data. Primary astrocytes from knock out mouse pups were isolated to ascertain whether the astroglyosis seen in these mouse models is a specific or non-specific consequence of altered oligodendrocyte structure. Cellometer analyzed spherical size of these cells during experimentation. Although the findings suggest it is a nonspecific response, more study is clearly needed. These findings also suggest a dissociation between [...]

By |2015-12-10T10:00:47+00:00December 10th, 2015|Categories: Cellometer User Publications|Tags: , |0 Comments

Cellometer X4 aides in assessing the toxic effects of silver nanoparticles

East Carolina University set out to elucidate the possible cardiovascular impacts that may occur after exposure to silver nanoparticles (AgNP), which are currently being assessed for use in a broad range of biomedical and consumer applications including wound dressings, laundry detergents, deodorants, and clothing. Despite their seeming utility, their downstream toxicity, especially in relation to the heart upon inhalation of aerosolized particles, has not been fully studied. Sprague Dawley rats were exposed to AgNP intratracheally for 7 days before branchoalveolar lavage was performed. The Cellometer Auto X4 was used on the contents of the resulting cell pellet to ascertain total [...]

By |2015-12-08T10:00:29+00:00December 8th, 2015|Categories: Cellometer User Publications|Tags: , |0 Comments