Establishing a High-throughput Screening Platform for NK mediated Cytotoxicity using Image Cytometry

Researchers highlight the need for large-scale screening of cytotoxic cells and their ability to kill target cell lines in both a 2D and 3D fashion.

Webinar on Demand: Immuno-Oncology Studies

Syngeneic tumor models and human immune cell-engrafted mice are two of the popular preclinical tools in immuno-oncology studies.

Video Workflow-at-a-Glance: NK Cell-Mediated Cytotoxicity Assay

Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging and analysis in 8 minutes.

Challenges Facing Immuno-Oncology Researchers with High-Throughput Cell Counting

This webinar explores options for researchers to perform high-throughput cell counting through assays such as MTT, Cell-Titer Glo and manually counting.

By |2021-06-15T04:54:15+00:00November 2nd, 2018|Categories: Celigo, Immuno-oncology, Instrument, Video|Tags: |0 Comments

Benefits of direct high-throughput cell counting in immuno-oncology research

By directly imaging and counting every cell in a well over a course of a drug treatment, the Celigo can perform the cytotoxicity assays in a label-free format.

Cellometer assists in identification of PGRMC as a cancer stem cell marker and therapeutic target

The University of Kentucky investigated progesterone receptor membrane component 1 (PGRMC1), an often upregulated component in thyroid, breast, colon and lung tumors. PGRMC1 has been associated with drug resistance and is thought of as an indicator of prognosis. The researchers employed a variety of cell types to represent head and neck cancers, as well as oral, lung and ovarian cancers. These cells were exposed to PGRMC1 inhibitors. The Cellometer performed cell counts with Trypan Blue. The PGRMC1 inhibitors successfully prompted cancer stem cell death even when other anti-cancer agents did not. The researchers suggest using PGRMC1 as a cancer stem [...]

Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines

South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]

Cellometer Vision participates in study to evaluate effects of lead nitrate on human leukemia cells

At the NIH-Center for Environmental Health (Jackson, MS), a study was designed to analyze the impacts of lead nitrate, a component that has been greatly reduced in paint and ceramic products, and yet its exposure levels in humans remain a concern. Because industrial sources of lead persist in our environment, these researchers evaluated its effects on the DNA damage, cell cycle arrest, and induction of apoptosis in human leukemia cells (HL-60). The Cellometer Vision was used to measure live and necrotic cells with propidium iodide. After exposure to lead nitrate, the cells showed significant increases in necrotic death, DNA damage, [...]

Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer

It's White Paper Wednesday! Read our featured white paper: Characterization of Breast Cancer Drugs via Mammosphere Morphometric Analysis Using Celigo Imaging Cytometer A panel of cytotoxic drugs, including doxorubicin and paclitaxel were used to study their effects on various breast cancer cell lines such as MDA-MD-436, MCF-7, SKBR3 and MDA-MB-231. Results show that the Colony Counting application can also be used to evaluate the clonogenicity and self-renewal of cancer stem/tumor-initiating cells by automatically analyzing mammosphere populations. The Colony Counting application on Celigo provides an efficient, reproducible and automated method for assessing the number, size, and morphology of cancer spheroids within [...]

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