Repurposing immune cells to develop new therapies

For the development of new drugs and the advancement of new cell therapies, pharmaceutical companies have recurred to cytotoxicity assays as a quick way to assess the viability of cell lines in response to an external stimulus.

Innovative Cytotoxicity Assays Utilizing Imaging Cytometry to Investigate Anti-Tumor Immunity

The ongoing pursuit of novel anti-cancer therapeutics must consider the potential for off-target effects on the immune system.

Establishing a High-throughput Screening Platform for NK mediated Cytotoxicity using Image Cytometry

Researchers highlight the need for large-scale screening of cytotoxic cells and their ability to kill target cell lines in both a 2D and 3D fashion.

Webinar on Demand: Immuno-Oncology Studies

Syngeneic tumor models and human immune cell-engrafted mice are two of the popular preclinical tools in immuno-oncology studies.

Video Workflow-at-a-Glance: NK Cell-Mediated Cytotoxicity Assay

Directly image and count calcein AM-labeled target cells in a 96-well plate using Celigo image cytometer. Concurrent imaging and analysis in 8 minutes.

Challenges Facing Immuno-Oncology Researchers with High-Throughput Cell Counting

This webinar explores options for researchers to perform high-throughput cell counting through assays such as MTT, Cell-Titer Glo and manually counting.

By |2021-06-15T04:54:15+00:00November 2nd, 2018|Categories: Celigo, Immuno-oncology, Instrument, Video|Tags: |0 Comments

Benefits of direct high-throughput cell counting in immuno-oncology research

By directly imaging and counting every cell in a well over a course of a drug treatment, the Celigo can perform the cytotoxicity assays in a label-free format.

Cellometer assists in identification of PGRMC as a cancer stem cell marker and therapeutic target

The University of Kentucky investigated progesterone receptor membrane component 1 (PGRMC1), an often upregulated component in thyroid, breast, colon and lung tumors. PGRMC1 has been associated with drug resistance and is thought of as an indicator of prognosis. The researchers employed a variety of cell types to represent head and neck cancers, as well as oral, lung and ovarian cancers. These cells were exposed to PGRMC1 inhibitors. The Cellometer performed cell counts with Trypan Blue. The PGRMC1 inhibitors successfully prompted cancer stem cell death even when other anti-cancer agents did not. The researchers suggest using PGRMC1 as a cancer stem [...]

Cellometer T4 supports research into the chemoprotective effects of aspirin in a variety of cancer cell lines

South Dakota State researchers investigated the role aspirin and its primary metabolite salicylic acid play as chemoprotective agents via the inhibition of cell cycle regulators cyclin A2 and CDK2. Using a variety of human cancer cell lines (HCT 116, HT-29, SW480, SK-MEL-28, SK-MEL-5, MDA-MB-231, MCF7, NCI-H226, OVCAR-3, PC-3, and B16-F10), the scientists investigated the effects the drugs had on cyclin A2 and CDK2 levels and activity. Floating and trypsinized cells were collected and analyzed for viability with Trypan Blue and the Cellometer Auto T4. In all the cell lines examined, aspirin and salicylic acid down regulated cyclin A2 and CDK2 [...]

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