Celigo Experiment Examples
Celigo Experiment ExamplesCeligo Experiment Examples are 2D and 3D assays that have been successfully performed on the Celigo Image Cytometer. This bench-top, bright field and fluorescent imaging system provides high speed, fully automated imaging and quantification of adherent and suspension cells in any format (6, 12, 24, 48, 96, 384, 1536-well plates, T flasks, and slides).
Celigo’s versatility allows for a wide range of assays that can be routinely and efficiently carried out during everyday lab operations. From cell line development to functional assays to 3D tumor spheroid analysis, there is no doubt that Celigo can help you take your experiments to the next level. Read or download any of the assays below to better understand how Celigo can be used in your lab.
Immuno-Oncology
Measure PBMC-mediated cytotoxicity by counting total live K562 cells over time in the presence and absence of IL2 ...
Measure antibody-dependent NK92 cell-mediated cytotoxicity using calcein AM-stained MDA-MB-231 ...
Measure NK cell-mediated cytotoxicity using calcein AM-stained K562 and IMR32 cells ...
Migration/Invasion
Validate wound healing migration assay with OrisTM Platypus plate on Celigo ...
Image and count the number of cells which have migrated through the membrane of a transwell insert using fluorescent detection of DAPI-stained cells ...
3D Models
Kinetically monitor multicellular tumor spheroid (MCTS) growth & Propidium Iodide (PI) dead cell signal on U87MG Glioblastoma over mulitple days ...
Measure the effects of a panel of drugs on the viability of U87MG Glioblastoma MCTS using calcein AM and Propidium Iodide fluorescent staining ...
To image and count whole well populations of organoids in a rapid manner without having to take multiple, time consuming Z-stacked images ...
Monitor the effects of a panel of drugs on the apoptosis of U87MG Glioblastoma MCTS using Caspase 3/7 and Hoechst fluorescent staining ...
Monitor the effects of a panel of drugs on the invasion of U87MG Glioblastoma MCTS into Basement Membrane Extract (BME) Matrigel ...
Using the bright field imaging, the Celigo will rapidly provide multicellular tumor spheroid images and diameters of treated U87MG MCTS ...
Using the bright field imaging, the Celigo will rapidly provide multicellular tumorspheroid images and diameters of treated MCTS in a 96-well plate ...
Fluorescent Assays
Measure cellular drug uptake in adherent and suspension cell cultures of a fluorescently-labeled drug at varying antibody concentrations ...
To analyze transfection efficiency over a 4 day period when treated with various amounts of a transfection compound ...
In this experiment, we demonstrate the capability of Celigo to measure cell count on microcarrier beads and infected cells positively staining for AlexaFluor488-labeled antibody again the viral protein ...
To measure the level of receptor internalization induced by known antibody ...
Celigo will be able to perform rapid, whole-well imaging and analysis of Ki-67 expression levels to compare between groups ...
By measuring the number of Caspase 3/7 positive cells, we can determine the counts of apoptotic cells in the population ...
Determine the counts of PI-positive cells kinetically at 24, 48 and 72 hours ...
Perform endpoint viability assay on MDA-MB-231 and K562 cells treated with Benzethonium for 24, 48 and 72 hours ...
Measure Jurkat cell concentration and viability at titrations of concentrations and different mixture of cell viability, which can demonstrate the high-throughput capability of Celigo image cytometer ...
Drug treatment will increase the percentage of DRAQ7-positive cells over time ...
Perform endpoint viability assay on MDA-MB-231 and K562 cells treated with Benzethonium for 24, 48 and 72 hours ...
By measuring the number of Caspase 3/7 positive cells, we can determine the percent of apoptotic cells in the population ...