Count 24 Samples in Less than 2.5 Minutes

Cellaca MX High-throughput Automated Cell Counter

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Cellaca Demo Video

See how fast you can count 24 samples

The High-throughput Automated Cell Counter that Changes Everything

With the new Cellaca MX High-throughput Automated Cell Counter you can now count up to 24 samples in 48 seconds using trypan blue or in 2.5 minutes with fluorescence.

Your workflow will be streamlined and more efficient when you integrate the Cellaca MX with these capabilities:

  • Batch samples in 24 well microplates for cell count and viability
  • Analyze cell lines or primary cells like PBMCs, T cells and tumor digests
  • Maintain cell integrity and minimize sample depletion during processing
  • Small sample size – only needs 25 µl of cells
  • Incorporate robotics with API compatible instrument
  • No instrument fluidics to maintain

You don’t have to limit your experimental design

Automated cell counters that require a lot of hands-on time with constant reloading of slides or that have the ability to only run in small batches can mean restricting the number of samples to be processed at a time and therefore set limitations on your experimental design. With the Cellaca MX Automated Cell Counter, you can run 24 samples at a time in 2.5 minutes or less, allowing you to expand the number of samples that can be counted in a fraction of the time.

High Accuracy at Fast Speeds

Number of Samples Amount of Time
Cellaca MX 24
480
48 seconds
30 minutes
Cell Counter V 12
480
36 minutes
24 hours

Cellaca’s ability to consistently image, decluster, and report cell viability and concentration make it a versatile instrument that can be used in multiple workflows. The ability to add additional automation with robotic integration can further increase productivity.

Cellaca MX Shows Close Correlation to Hemocytometer

Three tubes of 5-micron polystyrene beads were diluted to a concentration of ~ 2×106 beads/mL. To determine concentration within each tube, 40 individual hemocytometer counts were performed for each tube. The Cellaca MX was then used to measure the concentration on the same set of tubes.

  • The results show a very close correlation between the hemocytometer counted beads and the Cellaca MX counts
  • Notably, a total of 18 Cellaca plates (432 wells) over 3 individually counted Hemocytometer tubes all had average CV values of < 4%.
  • The measured concentration difference between the Cellaca MX and the Hemocytometer bead counts were between 0.7 – 2.3%
Callaca MX vs Hemocytometer
Hemo CV Cellaca CV Hemo Aveg Cellaca Aveg % Diff
Tube 1 4.46% 3.69% 2.03E+06 2.00E+06 1.20%
Tube 2 4.33% 3.64% 2.21E+06 2.16E+06 2.35%
Tube 3 4.28% 3.94% 2.10E+06 2.11E+06 0.71%

Consistent Counting (< 7% CV) of Trypan Blue Stained CHO Cells Over Multiple Experiments

consistent trypan blue cell counting

Figure 1. CHO cells stained with Trypan Blue were shown to have a CV of 7% or less over 4 independent experiments totaling 83, 24-well plates.

In an experiment, multiple users over four independent experiments were able to yield sample to sample variability for trypan blue stained CHO cells of less than 7% CV with a total of 1,992 samples counted (Figure 1). Cellaca’s high-speed imaging and data reporting allowed the researchers to complete 83 plates in under 70 minutes.

Analyze Low-volume Primary Cell Samples

Cellaca MX only needs 25 μL of cells, so you can analyze tumor biopsy tissue, mouse splenocytes, tumor infiltrating lymphocytes (TILs) and more.

Analyze Low-volume Primary Cell Samples

Human PBMC sample stained with Acridine Orange/Propidium iodide (AO/PI) and imaged using brightfield and fluorescence on Cellaca MX.

Count Only the Cells You’re Looking For

Use brightfield to check viability. Use fluorescence when you need specific details on cell health – tissue debris and red blood cells won’t artificially change your count.

Consistent Viability Measurement Using AOPI

Three-day old cultures of Jurkat and CHO-S cells were standardized to approximately 2×106 cells/mL. They were then both stained with Acridine Orange and Propidium iodide (AOPI) and analyzed on the Cellaca MX cell counter.

  • Bright field and fluorescent images were acquired for each channel and automatically saved. Shown above are representative bright field and AOPI counted images
  • Cellular counting and analysis are performed in the fluorescent channel while the bright field image is captured for documentation
  • Viability is automatically calculated and reported for each well. The average viability for 24 samples in plate 1 was 56% while the average viability for plate 2 was 53%
  • The standard measured deviation between the combined 2 plates was 4%.
  • This data shows excellent and consistent viability readings for low viability samples
Jurkat and CHO cell imaged by Cellaca
Jurkat Cell Viability Data by Cellaca

Highly Reproducible Results

Cellaca MX results are highly reproducible and linear across a 2-log cell concentration range. You’ll also get consistent counts across systems, labs and users. That means you can run a sample once and know you’ve got the right result the first time.

Serial dilution of Jurkat cells

Serial dilution of Jurkat cells from 1.8E+07 to 1.8E+05 cells/mL analyzed on Cellaca MX. Counts were linear across the entire concentration range with CVs <2.4% for mean cell concentrations of 1.6E+07 cells/mL or higher, and <0.7% for 1.2E+07 cells/mL and below (n=9).

Cellaca data

Mix the Sample within the Plate

Nexcelom’s unique proprietary patented technology allows the user to mix the cell sample and the viability dye or staining reagent in a single well within the plate. The built-in capillary mechanism ensures even distribution across the wells. Therefore, providing a completely processed sample set for imaging, accurate counting, and analysis. There is no longer the need to perform these steps in a separate tube.

The Nexcelom plate for Cellaca has 24 chambers for cell samples

Cellaca MX Specifications

The Cellaca MX is available in different configurations. Each is field upgradeable to allow your instrument to configure to your experimental needs and quickly drive to deeper cellular insights.

Cellaca MX BF Cellaca MX FL2 Cellaca MX FL5
Channels Brightfield Brightfield, Green, Red Brightfield, Blue, Green, Red, Far Red
Number of Channels 1 4 14
Excitation LED N/A 470, 527 365, 470, 527 and 620 nm
Emissions Filters N/A 534, 655 452, 534, 605, 655 and 692 nm
Commonly Used Compatible Dyes Trypan Blue Trypan Blue, AO/PI Trypan Blue, AO/PI, Hoechst, DAPI, PE, APC, FITC, CFDA, Calcein AM, 7AAD
Counting Speed Per 24 Samples 48 seconds 2.5 minutes 2.5 minutes
Volume (per well) 25 µL – 100 µL sample volume
50 µL – 200 µL total well volume
25 µL – 100 µL sample volume
50 µL – 200 µL total well volume
25 µL – 100 µL sample volume
50 µL – 200 µL total well volume
Size/Diameter Range 5 – 80 µm 5 – 80 µm 5 – 80 µm
Concentration Range 1×105 – 1×107 1×105 – 1×107 1×105 – 1×107
Fluorescence Upgradeable Yes Yes (add colors) N/A
Robotic Compatible Yes Yes Yes
Dimensions 13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
Weight 40 lbs (18 kg) 42 lbs (19 kg) 42 lbs (19 kg)

Customer Testimonials

So amazing!

So amazing! No instrument fluidics are to be maintained, it analyses ce...

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Ernest Cook Mengo
Jun 3, 2020

So amazing!

So amazing! No instrument fluidics are to be maintained, it analyses cell lines or primary cells like T cells, PBMCs, and tumor digest, incorporate robotic with API compatible instrument. It [Cellaca MX] is fast enough to count 24 samples in less than 2.5 minutes. There is value for money in general!

Ernest Cook Mengo
Jun 3, 2020

Superfast

Superfast counting, easy to handle, accurate results [with Cellaca MX]....

Level Biotech
Jun 3, 2020

Superfast

Superfast counting, easy to handle, accurate results [with Cellaca MX].
Level Biotech
Jun 3, 2020

10x Faster than Manual Counting

Easy to use, the effectiveness is high enough. Results are reproducible that is important for experi...

more
Qualipharm
Jun 3, 2020

10x Faster than Manual Counting

Easy to use, the effectiveness is high enough. Results are reproducible that is important for experiments. This instrument [Cellaca MX] greatly increases the consistency of our experiments. And 10x faster than manual counting, no need for washing or contamination, so it will be excellent for decreasing of time-consuming and increasing the accuracy.
Qualipharm
Jun 3, 2020

Accurate

[Cellaca MX] Super easy to use and fairly high throughput. Great value and accurate cell counts!!!...

Laboratory Manager
Jun 3, 2020

Accurate

[Cellaca MX] Super easy to use and fairly high throughput. Great value and accurate cell counts!!!
Laboratory Manager
Jun 3, 2020

Amazing

Amazing cell counting in a few minutes, many samples collected at the same time [using Cellaca MX]....

AbbVie
Jun 3, 2020

Amazing

Amazing cell counting in a few minutes, many samples collected at the same time [using Cellaca MX].
AbbVie
Jun 3, 2020

Solid, reliable

We were rapidly able to use the Cellaca MX for pipeline PBMC immunology assays integrating both flow...

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Allen Institute for Immunology
Jun 3, 2020

Solid, reliable

We were rapidly able to use the Cellaca MX for pipeline PBMC immunology assays integrating both flow cytometry and single-cell genomics platforms. The nuclear staining was useful to exclude RBCs from the cell count. The ability to run a batch of 24 samples in a single plate has been integrated into our data analysis streams. Applications scientists from Nexcelom have been extremely helpful in customizing instrument settings for our specific needs. Solid, reliable cell counter with excellent support from the manufacturer.
Allen Institute for Immunology
Jun 3, 2020