Count 24 Samples in Less than 2.5 Minutes

Cellaca MX High-throughput Automated Cell Counter

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See how fast you can count 24 samples

The automated cell counter that changes everything

With the new Cellaca MX High-throughput Automated Cell Counter you can now count up to 24 samples in 48 seconds using trypan blue or in 2.5 minutes with fluorescence.

Your workflow will be streamlined and more efficient when you integrate the Cellaca MX with these capabilities:

  • Batch samples in 24 well microplates for cell count and viability
  • Analyze cell lines or primary cells like PBMCs, T cells and tumor digests
  • Maintain cell integrity and minimize sample depletion during processing
  • Incorporate robotics with API compatible instrument
  • No instrument fluidics to maintain

Analyze low-volume primary cell samples

Cellaca MX only needs 25 μL of cells, so you can analyze tumor biopsy tissue, mouse splenocytes, tumor infiltrating lymphocytes (TILs) and more.

Cellaca counted human PBMCs
Human PBMC sample stained with Acridine Orange/Propidium iodide (AO/PI) and imaged using brightfield and fluorescence on Cellaca MX.

Count only the cells you’re looking for

Use brightfield to check viability. Use fluorescence when you need specific details on cell health – tissue debris and red blood cells won’t artificially change your count.

Brightfield and fluorescent images of PBMCs. In brightfield, white blood cells, platelets, and red blood cells are seen. Nuclear fluorescent staining using both green and red channels accurately identifies only the white blood cells.

You don’t have to limit your experimental design

Automated cell counters that require a lot of hands on time with constant reloading of slides or that have the ability to only run in small batches can mean restricting the number of samples to be processed at a time and therefore set limitations on your experimental design. With the Cellaca MX Automated Cell Counter you can run 24 samples at a time in 2.5 minutes or less, allowing you to expand the number of samples that can be counted in a fraction of the time.

High accuracy at fast speeds

Cellaca’s ability to consistently image, decluster, and report cell viability and concentration make it a versatile instrument that can be used in multiple workflows.

In an experiment, multiple users over four independent experiments were able to yield sample to sample variability for trypan blue stained CHO cells of less than 7% CV with a total of 1,992 samples counted (Figure 1). Cellaca’s high speed imaging and data reporting allowed the researchers to complete 83 plates in under 70 minutes.

Consistent Counting (< 7% CV) of Trypan Blue Stained CHO Cells Over Multiple Experiments

Cellaca MX consistency data
Figure 1. CHO cells stained with Trypan Blue were shown to have a CV of 7% or less over 4 independent experiments totaling 83, 24-well plates.

Count once and done

Cellaca MX results are highly reproducible and linear across a 2-log cell concentration range. You’ll also get consistent counts across systems, labs and users. That means you can run a sample once and know you’ve got the right result the first time.

Cellaca Dilution Factor Data
Serial dilution of Jurkat cells from 1.8E+07 to 1.8E+05 cells/mL analyzed on Cellaca MX. Counts were linear across the entire concentration range with CVs <2.4% for mean cell concentrations of 1.6E+07 cells/mL or higher, and <0.7% for 1.2E+07 cells/mL and below (n=9).
Cellaca data

Mix the sample within the plate

Nexcelom’s unique proprietary patented technology allows the user to mix the cell sample and the viability dye or staining reagent in a single well within the plate. The built-in capillary mechanism ensures even distribution across the wells. Therefore, providing a completely processed sample set for imaging, accurate counting, and analysis. There is no longer the need to perform these steps in a separate tube.

How Cellaca plates work
The Nexcelom plate for Cellaca has 24 chambers for cell samples

Available Configurations

The Cellaca MX is available in different configurations. Each is field upgradeable to allow your instrument to configure to your experimental needs and quickly drive to deeper cellular insights.

Cellaca MX BF Cellaca MX FL2 Cellaca MX FL5
Channels Brightfield Brightfield, Green, Red Brightfield, Blue, Green, Red, Far Red
Number of Channels 1 4 14
Excitation LED N/A 470, 527 365, 470, 527 and 620 nm
Emissions Filters N/A 534, 655 452, 534, 605, 655 and 692 nm
Commonly Used Compatible Dyes Trypan Blue Trypan Blue, AO/PI Trypan Blue, AO/PI, Hoechst, DAPI, PE, APC, FITC, CFDA, Calcein AM, 7AAD
Counting Speed Per 24 Samples 48 seconds 2.5 minutes 2.5 minutes
Volume (per well) 25 µL – 100 µL sample volume
50 µL – 200 µL total well volume
25 µL – 100 µL sample volume
50 µL – 200 µL total well volume
25 µL – 100 µL sample volume
50 µL – 200 µL total well volume
Size/Diameter Range 5 – 80 µm 5 – 80 µm 5 – 80 µm
Concentration Range 1×105 – 1×107 1×105 – 1×107 1×105 – 1×107
Fluorescence Upgradeable Yes Yes (add colors) N/A
Robotic Compatible Yes Yes Yes
Dimensions 13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
13 in x 13 in x 16 in
(33 cm x 33 cm x 41 cm)
Weight 40 lbs (18 kg) 42 lbs (19 kg) 42 lbs (19 kg)
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“The Cellaca MX has dramatically reduced sample processing time for our ex vivo preparations.  What used to take us 5 people and 7 hours, now takes us only 3 people and 4 hours to process the samples, count the cells and plate them for downstream assays.  Not only has the Cellaca MX saved us time and increased our efficiency, but it also has improved our sample quality.  Cells no longer sit on ice waiting to be counted, making the data output for our proteomic, genomic, and flow cytometry applications more accurate.”

Dr Kristie Barth, Immunology Scientist, Evelo Bio, MA Cambridge

“Scientific Directors approved [Cellaca] … they will get an approval from Dr. Allison and then we can move forward with this purchase. I am so happy!!!”

Renata S. Collazo, MSc, Research Laboratory Coordinator, MD Anderson Cancer Center