Count messy samples more accurately with fluorescence! With the Cellaca MX 2FL and 5FL models, you can eliminate red blood cells, platelets and debris from your counts using fluorescent nuclear stains. Acridine orange and propidium iodide are perfect for counting the nucleated cells in samples like PBMCs, patent samples, tumor digests and samples with lots of debris.
The advantage of fluorescent counting for primary cells
The images (Figure 4) demonstrate the advantage of fluorescent counting for primary cells. The bright field image shows the combination of nucleated cells, red blood cells and platelets present in the sample. Only the live and dead nucleated cells are visualized and counted in the green and red fluorescent channels.