Transcript: Cellometer Auto 2000 Assay Overview
The Cellometer Auto 2000 is an automatic cell viability counter with dual fluorescence.
The system provides consistent, reliable and accurate results for primary cells.
The instrument comes pre-configured with seven different assay types, all of which can be customized by the user if desired.
In clean samples, the bright field imaging mode is used to determine total concentration. Live cell concentration and viability are obtained by adding trypan blue.
With clean sample conditions, live cells are measured using bright filed. Viability is obtained using the fluorescent nuclear acid staining dye propidium iodide for dead cells.
A Specially designed assay to measure low cell concentrations using acridine orange and a much deeper cell counting chamber is also included.
Samples containing a large number of red blood cells, such as whole blood, cord blood, and bone marrow or isolated cells such as PBMC are stained with a mixture of acridine orange and propidium iodide. Fluorescence or the combination of bright field and Fluorescence are used to obtain live/dead cell concentrations and viability.
The combination of bright filed plus dual fluorescence are used to measure concentration and viability of stem cells, primary cells and cell lines in messy samples using acridine orange and propidium iodide. Users can set up any assay to measure # of total cells, fluorescent cells, and % of fluorescent cells, such as an assay Measuring GFP Transfection efficiency.