It’s White Paper Wednesday! Read our featured white paper: Comparing Fluorescence-Based Viability Detection Method using the Cellometer Vision
In this work, Cellometer Vision was employed to demonstrate rapid fluorescence-based
viability measurements and the comparison of various fluorescent staining methods. First, fluorescent nucleic acid stains that examine membrane integrity were tested and validated by comparing against the standard trypan blue exclusion method. Similarly, fluorescent enzymatic stains that examine metabolic activities were tested and validated against the standard trypan blue exclusion method. Third, nucleic acid and enzymatic stains were compared by measuring viabilities of Jurkat cells incubated at different temperatures of water bath.