Trypan Blue and Fluorescence-Based Viability Detection Methods Via Morphological Observation

It’s White Paper Wednesday! Read our featured white paper: Comparison of Trypan Blue and Fluorescence-Based Viability Detection Methods Via
Morphological Observation

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These studies reveal that over a time course analysis in standard cell culture, TB exclusion reports more viability in comparison to the fluorescence-based tools, although the studies did not speculate as to the reasoning behind the differences noted (Jones and Senft 1985; Mascotti et al. 2000). In this work, we perform a head-to-head comparison between cell viability as determined by TB and that determined by AO/PI using an automated image-based cytometer, the Cellometer. Image-based cytometry allowed us to examine the morphological changes that occur in TB-stained cells, and these may explain the differences in viability counts reached by the two methods.

 

 

Tb vs FL
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About the Author:

Leo Chan
Dr Leo Chan currently serves as the Technology R&D Manager at Nexcelom Bioscience LLC, Lawrence, MA. His research involves in the development of instrument and applications for the Celigo and Cellometer image cytometry system for detection and analysis of cells for oncology and immunology research. He is a member of the American Association of Cancer Research and the American Association of Immunologists. He received his B.S., M.S., and Ph.D. in Electrical and Computer Engineering from the University of Illinois at Urbana-Champaign (2000-2008).

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