Here’s another great example of how researchers across the world are using the Cellometer instruments before performing their downstream assays:
Researchers at the French Institute for Health and Medical Research and the University of Lyon investigated the effects of ultrasound on sonoporation (increasing a cell’s permeability in order to transfer molecules or genetic material into the cells). Using adherent HT-29 cells, as well as the Cellometer Auto T4 and trypan blue, researchers evaluated the cells’ viability 24 hours prior to sonication studies. Varying the acoustic intensities and cavitation regulation allowed them to optimize sonoporation efficiency, regardless of ambient temperature. The optimization worked equally well on adherent and suspension cells and required no ambient or media temperature regulation, a necessity with other sonoporation techniques. This “real-time” sonoporation control would allow researchers the freedom to perform efficient sonoporation at a lab bench, rather than within the confines of an incubator.