The optimized detection method was then employed for performing a high-throughput microneutralization screening of potential FCoV antibodies using five collected feline sera against the Feline coronavirus strain FCoV-WSU-79–1146. The high-throughput neutralization assay was conducted twice. Due to the lack of cross-neutralization between FCoV type I and II viruses from the feline sera, no viable candidates were identified.
Perform a Microneutralization Assay in Approximately 15 Minutes per 96-well Plate
Traditional methods for screening vaccine and therapeutic candidates as well as assessing immune responses are typically time-consuming, low-throughput, and can require several days. Lack of digital autosave makes recordkeeping problematic and repeatability is also a concern with multiple users producing varying results. The Celigo saves time by simultaneously imaging and analyzing data with digital autosave that makes recordkeeping effortless. Additionally, preset features save time, resources, and prevent extended user training or operator variability issues. “The image cytometer performed the neutralization assay at approximately 15 min per 96-well plate for simultaneously acquiring and analyzing whole-well images in brightfield and fluorescence, equivalent to ~9 s per sample.”
High-throughput viral microneutralization method for feline coronavirus using image cytometry
First Author: Morgan Pearson, Department of Microbiology, Immunology, and Pathology
First Author Institution: Colorado State University, Fort Collins, CO, 80523, United States
Journal: Journal of Virological Methods