Here’s a great example of how the Celigo image cytometer is able to perform common experiments while saving time and money!
Ignyta, Inc. was looking for a new way to perform reagent-free proliferation analyses with suspension cells. This new method had to produce results which correlated well to their current method, Cell Titer-Glo®. Nexcelom and Ignyta partnered to perform a head-to-head cell proliferation comparison between Celigo® and Cell Titer-Glo. Using four suspension cell types (Ba/F3 parental cell line, Ba/F3 expressing an oncogenic gene, oncogenic gene mutant A and B), Ignyta plated all cells at a concentration of 5,000 cells/well in the presence of various concentrations of four drugs (1-4). Three days later, Celigo evaluated cell proliferation using bright field to capture and analyze whole-well images in less than 5 minutes per plate. The exact same plates were then used to perform the standard (“lyse and read”) Cell Titer-Glo assay.
The two methods produced IC50 values that were highly comparable across all cell types and drug concentrations (r2=0.998). Celigo proved to be a rapid, accurate analysis for Ignyta’s suspension cells, providing images for visual verification and a reagent-free method which meant the same cells could be analyzed repeatedly over a time course. Now, Ignyta had a simplified process by which to analyze cell proliferation, one that was highly correlated to their previous method and required no reagents or additional incubation times, saving time, cost, and supplies.
If you are interested to discuss how the Celigo could benefit your research, contact us at info@nexcelom or visit our website for more information.