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Direct Cell Counting Assays for Immuno Therapy

Cytotoxicity assays play a central role in studying the function of immune effector cells such as cytolytic T lymphocytes (CTL) and natural killer (NK) cells. Traditionally, cytotoxicity assays have been performed using Chromium-51 (51Cr) and Calcein release assays.

The assays involve labeling tumor cells (target) with radioisotope or fluorescent dyes, when the target cells are subjected to CTLs or NK cells (effector) mediated killing, they release the entrapped labels into the media. The amount of released label in the media is measured to determine the level of cytotoxicity the effectors have induced.

A novel cytotoxicity assay using the Celigo imaging cytometer to directly count live, fluorescently labeled target cells has recently been introduced.

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