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Automated Counting and Sizing Freshly Isolated Adipocytes with Minimal Sample Preparation

Cellometer Vision incorporates image based cell counting
and fluorescence detection in a compact and easy-to-use
instrument. With fluorescence detection capabilities,
Cellometer Vision is an ideal solution for counting complex
samples with minimal sample preparation, such as counting
and sizing adipocytes.


The size and number of adipocytesare directly associated
with adipose tissue metabolism. However, currently available
automatic cell counting methods often require complex
sample preparation and use of hazardous reagents. With a
traditional manual hemacytometer method, there is a
technical challenge in distinguishing intact adipocytesfrom
lipid droplets generated from ruptured adipocytesin the
collagenasedigestion process. Cellometer Vision incorporates
both total cell counting and fluorescence detection in one
easy-to-use instrument. By instantly staining enzymatic
treated fat tissue samples with acridineorange (AO), a DNA
fluorescence dye, Cellometer Vision can detect and analyze
fluorescent positive adipocytes. In less than a minute, the
system can generate accurate cell counting results and also
report cell sizes. Various primary adipocytes, such as human,
mice, rat, etc. with diameters of 25um to 250um can be easily
loaded into a disposable counting chamber designed
especially for adipocytesto eliminate liquid handling reliability
issues.

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