Cell Counting Repeatability and Consistency
Cell counting accuracy is governed by two major factors. The first one is the
homogeneity of cell suspension when a sample is taken for measurement. Typical mammalian cells do not suspend in solution. Without thorough mixing and declumping procedure, a sample taken for cell concentration measurement will not reflect cell concentration in the original sample.
When a hemacytometer is used, after the sample is loaded, the operator has to make judgment under a microscope. The variation in judgment from person to person will also affect cell concentration and viability.