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Cell cycle analysis on Celigo

Analysis of the cell cycle is often used in drug screening to discover compounds that affect the proliferation and growth of
cells. Mitosis is composed of the G0/G1-, S-, and G2-phases,
which can be distinguished and quantified by antibody staining and imaging. While this type of assay is usually performed
by flow cytometry, we present here the use of the Expression
Analysis application on the Celigo adherent cell cytometer to
monitor each phase of the cell cycle. The ability to analyze cell
cycle progression of adherent cells directly in multi-well plates
greatly facilitates the implementation of this assay in compound
screens, significantly reduces the number of cells and reagents
required for each data point, and eliminates cell physiology impacts caused by trypsinization and suspension of adherent cells.


The Celigo cytometer is a novel imaging platform that combines
brightfield and fluorescent imaging with rapid full-well acquisition of a variety of well formats (1536- to 6-well plates). Celigo
optics provide uniquely uniform illumination throughout the
entire well with excellent image contrast right to the edge of the
well. These capabilities enable the identification of cells anywhere in the well with accurate fluorescence quantification. The
cell cycle assay presented in this application note is a 2-color fluorescent assay using the blue and green channels of the Celigo
cytometer.

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