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  5. Assay Protocol: Automated (Auto 2000) vs. Manual Cell Counting

For researchers performing manual cell counting, a key factor involved in evaluation of an automated cell counter is comparison to their current method. Based on our experience supporting thousands of Cellometer customers in the field, we have designed the following protocol to make comparison of the Cellometer Auto 2000 Cell Viability Counter to manual hemacytometer results as accurate as possible. For the comparison to be valid, Auto 2000 and hemacytometer counts must be done at the same time using aliquots from the same cell sample.

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