Cell Viability Analysis using Calcein AM, Propidium Iodide and Hoechst
Application: Cell viability analysis using Calcein AM, Propidium Iodide and Hoechst on A549 cells.
Description: This protocol describes the analysis of cell viability on A549 cells using Calcein AM as a measure of enzymatic activity in live cells, Propidium Iodide as a measure of dead cells with compromised membrane integrity and Hoechst as a marker for all nucleated cells. The application reports the counts and percentages of live, dead and total numbers of cells.
Celigo Application: Cell Viability (Live + Dead + Total).
Plate Type: Greiner cat# 655090 black wall clear bottom 96-well Plate.