Can I use the Cellometer to count adipocytes? Can I measure viability?
Yes! The first thing to note is that when working with adipocytes, you will need to use our CHT4-PD300 slides. These slides are specifically designed for larger cells. Secondly, you will need to use a combination of Calcein-AM and propidium iodide (PI) in order to measure viability. Calcein-AM is a stain that will fluoresce green in metabolically active cells. The PI, however, can only enter cells with compromised cell membranes (dead/dying cells) and will fluoresce red. The cells that fluoresce green indicate that they are metabolically active and alive, and the cells that fluoresce red indicate that they are dead cells. The catalog number for this kit is CSK-0118.