How do I deal with clumpy cells?
There are a few ways to address clumpy samples. The first way is to make sure your sample has been adequately mixed using a pipette or a gentle vortex. After you’ve loaded the sample, make sure that your focus shows crisp, dark membranes, especially within the clusters. We suggest you first run a count and then visually QC the images using the “Counted” function. This will show you how the instrument is parsing the cells in these clusters. If you are not satisfied with the way that the software is de-clustering, please give Nexcelom Support a call or email. We can set up a remote session or a phone call to help analyze your sample with you to optimize the de-clustering parameters.