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Concentration and Viability Measurement of Yeast in Corn Mash using the Cellometer Vision

Leo L. Chan, Emily J. Lyettefi, Alnoor Pirani, Tim Smith, Bo Lin, and Jean Qiu

We demonstrate a novel imaging cytometry method for concentration and viability measurement of yeasts in corn mash directly from operating fermenters. It employs an automated cell counter, a proprietary dilution buffer and staining solution from Nexcelom Bioscience to enumerate yeasts in corn mash. This novel method provides an essential tool for biofuel industries in United States to efficiently monitor yeast viability during fermentation process to ensure consistent bioethanol output.

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