Home/Poster: Obtaining Consistent and Accurate Cell Counting Results with Cellometer Automatic Cell Counters
  1. Home
  2. Knowledge Base
  3. Scientific Library
  4. Posters
  5. Poster: Obtaining Consistent and Accurate Cell Counting Results with Cellometer Automatic Cell Counters

Obtaining Consistent and Accurate Cell Counting Results with Cellometer Automatic Cell Counters

Bo Lin, Tim Smith, Alnoor Pirani, Jean Qiu, and Peter Li

Demand for Cell-based assays are increasing in all
biomedical research and drug discovery and development
fields. Rapid and accurate characterization of cell population in
a sample contributes significantly to assay validity. However,
current available automatic cell counting methods often require
costly instruments with on-board liquid handling. Daily usage
requires special reagent and sample preparation, along with
routine calibration and maintenance. Due to these limitations,
cell counting remains largely on a traditional, manual process
using hemacytometers in most biomedical and pharmaceutical
laboratories. We have developed the Cellometer line of
automatic cell counting instruments, that aim to replace
tedious manual cell counting processes. In comparison with
traditional hemocytometers, we found that there is high
correlation for measuring cell concentration and trypan blue
viability between Cellometer automatic cell counters and
hemocytometers (R2>0.98). Using series diluted red blood cell
samples, we found that Cellometer generated reliable results
from cell concentration as low as 1.0 X 105 to 1.5 X107 cells/ml.
Experimental results indicated that Cellometer can be used to
accurately count clumpy cells and irregularly shaped cells.
With proprietary algorithms, the system can specifically
analyze subpopulations of cells from heterogeneous biological
samples and eliminate cell/tissue debris by size and shape
exclusion. We also developed the Cellometer Vision system to
automatically analyze fluorescent properties of cells. By
combining bright field and fluorescence microscopic
characteristics, Cellometer Vision can be applied in many
complex cell population characterization assays. Examples
include: rapid analysis of GFP transfection rates, direct
counting acridine orange stained white-blood-cells from whole
blood samples, determining cell viability using propidium
iodide (a membrane impermeable dye), specific counting and
size measurement of adipocytes with bodipy dye, and
quantitative analyzation of Jurkat apoptosis with FITC
conjugated annexin-V.

Was this article helpful?

Related Articles

Go to Top