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ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture with Hoechst for Celigo

CSK-V0003-1

In real-time, measure apoptosis by detecting Caspase 3/7 in 2D live cell culture and 3D tumor spheroids and determine total number of cells using Hoechst.

Nexcelom’s ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture kit is designed to detect caspase 3/7 activity in live cells. The ability to perform kinetic apoptosis assays allows researchers to continuously measure the caspase 3/7 activity within the cell population. This no wash assay has been shown to effectively work in both 2D and 3D cultures.

The reagent (NucViewTM) consists of a nucleic acid-binding dye with a fluorescent probe that is attached to a four-amino acid peptide sequence DEVD (Asp-Glu-Val-Asp) forming a cell membrane-permeable DEVD-DNA complex. While the nucleic-acid dye is linked to the DEVD peptide sequence, the dye is
unable to bind to DNA and remains non-fluorescent. During apoptosis, caspase 3/7 proteins cleave the DEVD-DNA dye complex and thereby release the high-affinity DNA dye, which translocates to the nucleus and binds to the DNA, producing a bright green fluorescent signal.

Learn more about Apoptosis Reagents

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