The ViaStain™ PI Staining Solution enables the user to quantitatively distinguish live and dead cells in pure cultures using the Cellometer system. Propidium iodide (PI) is a membrane exclusion nucleic acid dye that only enters cells with compromised membranes (dead cells). When cell counting is performed using both bright-field and fluorescent imaging, cells that fluoresce in red are counted as dead, while the bright-field image provides the total cell count. The Cellometer software utilizes the total cell count and dead cell count to calculate percent viability.
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