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Concentration and Viability Measurement of Canine Stromal Vascular Fractions using Cellometer Vision

The use of mesenchymal stem cells (MSCs) in regenerative medicine holds great promise for tissues damaged by a number of acute conditions, such as injuries to tendons, ligaments, bone or cartilage and for chronic conditions such as osteoarthritis. MSCs obtained from the stromal vascular fraction (SVF) of adipose tissue, possess multi-lineage differentiation capacity, which allows them to develop into a variety of cell types, including chondrocytes, osteoblasts, myocytes cells and others [1-11]. Accurate determination of cell concentrations and viability in freshly isolated adipose SVF is critical in order to achieve the expected basic or clinical research outcomes.

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