Measure total nucleated cells collected by Apheresis

Apheresis is the process of removing a specific component of the blood, such as platelets, red blood cells, plasma (liquid part of the blood) or granulocytes (white blood cells) and returning the remaining components to the donor.

Leukapheresis is a specific kind of apheresis in which white blood cells  are separated from a sample of blood. Cells contained in a leukapheresis product is mainly nucleated cells, some residual red blood cells and platelets.

  • Application: measure live /dead nucleated cell concentration and viability
  • Sample Type: human peripheral blood Leukapheresis
  • Cell Type: total nucleated cells
  • Staining Method: acridine orange and propidium iodide (Ao/PI)
  • Instrument: Cellometer Auto2000
  • Cell Images: bright field / green fluorescence from live cells / red fluorescence from dead cells

Major Steps:

  • Mix BM sample with staining solution with 10 fold dilution
  • Load 20 ul stained BM sample into a counting chamber
  • Insert into Auto2000
  • Display cell image and count
  • Obtain live / dead and TNC concentrations, viability
Cell Images of Leokapheresis Sample with bright field, live cells in green fluorescence and counted TNC

Cell Images and Total Nucleated Cells Counted by Auto2000

Results:

  • Total nucleated cells counted: 2493
  • Live cells counted: 2381
  • Total nucleated cell concentration: 4.36E7 (cells/ml)
  • Live cell concentration: 4.17E7 (cells/ml)
  • Viability: 95.5%
By | 2012-05-01T13:13:06+00:00 May 1st, 2012|Categories: Cellometer Application News|0 Comments

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