Cellometer Auto T4 Automated Cell Counter 2018-06-26T16:40:36+00:00

Cellometer Auto T4 Bright Field Cell Counter

Automated cell counter for trypan blue viability for cell lines

Why Auto T4?

  1. 1-Step Concentration & Viability
  2. Analyze < 99% of Mammalian Cells including clumpy MCF-7
  3. Cell Size Analysis
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The Cellometer Auto T4 cell counter has made cell counting simpler and more standardized. A tedious job has been eliminated. Thanks Nexcelom!

John Sipes, NCI

Our Cellometer Auto T4 has continued to be a time saver and make cell counting less of a headache! Being able to program different cell types is also a great feature.

Colleen Worne, University of Utah

The Cellometer Auto T4 has changed the way I count cells and I couldn’t be happier about it. No more painstaking hand counting using the hemacytometer. Thanks so much Nexcelom!

Solomon C. Amadiume, Weill Cornell Medical College

Our customers include:

Cellometer Auto T4 customers

 

Introduction to the Cellometer Auto T4 Bright Field Cell Counter

Cell Counting Made Simple

Automatic Cell Counting Made Simple with the Cellometer Auto T4

Simple, Automated Cell Counting in 30 Seconds

The Auto T4 utilizes bright field imaging and pattern-recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

Cell counting results in less than 30 seconds

Load Sample, View Image, Count Cells, and Obtain Results in < 30 seconds

The Auto T4 Allows Hemacytometer Users to:

  • Increase throughput
  • Improve consistency
  • Ensure all data is correctly captured
  • Count difficult cells (clumpy, irregular-shaped)
  • Eliminate judgment errors, miscounts, and user-to-user variability

One-step Concentration & Viability

Trypan blue viability with Cellometer Auto T4

The Auto T4 simultaneously calculates cell concentration and % viability for cultured cells stained with trypan blue.

Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. The dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging.

Cellometer Auto T4 instrument report

Within 30 seconds, the Auto T4 instrument reports:

  • Live, dead, and total cell count
  • Live and total cell concentration
  • Mean Cell Diameter
  • % Viability

Counted bright field Image

Counted bright field Image from Cellometer Auto T4

Live cells = green circle
Dead (stained) cells = red circle

Analyze >99% of Cultured Mammalian Cells, including NCI-60 and clumpy MCF-7 cells

NCI-60 is a group of 59 human cancer cell lines (originally 60) developed by the National Cancer Institute for screening purposes. Isolated from various tissues (bone marrow, lung, ovary, brain, skin, breast, colon, kidney, prostate), the cell lines are used extensively to screen experimental cancer drug compounds and study tumor development.

Irregular-shaped Cells

Irregular-shaped Cell image from Cellometer Auto T4

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells and activated T-cells.

Clumpy Cell image from Cellometer Auto T4

Clumpy Cells

The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown at left).

  • 57% of the NCI-60 cell lines are clumpy, contain debris, or display large variations in cell shape or size.
  • All 59 NCI-60 cell lines have been successfully validated on the Cellometer Auto T4 Cell Counter.

Cell Size Analysis & Size-based Counting

cell size histogram based on cell diameter using the Cellometer Auto T4

The Auto T4 Cell Counter Automatically generates a cell size histogram based on cell diameter.

Because Cellometer generates individual cell size measurements, multiple samples can be overlaid on one histogram enabling analysis of the change in cell diameter over time.

Images from experiments involving SF9 or SF21 cells from the Cellometer Auto T4

For experiments involving SF9 or SF21 cells, saved images can be viewed and re-counted to analyze changes in morphology over time.

Cellometer auto T4 image showing cell size of dendritic cells

The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.

This example demonstrates counting of mature dendritic cells cultured from PBMCs based on cell diameter.

Cell Images for Data Verification

Cellometer auto T4 imaging HEK293 and HeLa cells countedNo two cells are the same. With the Auto T4 Cell Counter, cell morphology can be immediately viewed on-screen.

Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly.

Users can confirm that:

  • Cells are counted correctly, based on size and shape
  • Debris is excluded
  • Cells within clumps are being counted individually
  • Cell images can be archived and exported for use in publications and presentations.
  • Saved images can be re-counted using default or user-optimized analysis settings.

Exclusion of Debris

Exclusion of Debris from Cellometer Auto T4

Because Cellometer recognizes cells based on size, brightness, and morphology, cellular debris is easily and accurately excluded from counting results.

  • Cell size parameters can be modified to optimize exclusion of debris from results and enhance the accuracy of counting for a wide range of cell sizes.
  • The counted image can be viewed to verify exclusion of debris from results.

10x Faster and More Precise than Manual Counting

Hemacytometer under microscope, using Cellometer Auto T4

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto T4 Cell Counter calculates cell count and concentration for live and dead cells and % viability in just 30 seconds.

Cellometer Precision

The Cellometer %CV (Coefficient of Variation), including sample preparation, was well under 5% for most cell lines tested. The CVs for manual counts ranged from 7% to 20%. Automated cell counters remove inter-operator variability and subjectivity from cell counts to generate more precise data.

Cellometer Auto T4 vs Hemacytometer graph

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Eliminate Recording & Calculation Errors
  • Reduce Counting Time … Run More Experiments
Cellometer Auto T4 Hemacytometer
Concentration n CV Concentration  n  CV
Jurkat 1.17 x 106 4 2.27% 1.11 x 106 3 8.04%
YAC-1 1.36 x 106 4 3.17% 1.41 x 106 3 11.89%
K562 6.36 x 105 4 8.29% 5.58 x 105 3 9.66%
H460 1.32 x 106 4 5.57% 1.16 x 106 3 20.65%
SW620 3.81 x 105 4 2.79% 3.70 x 105 3 19.49%
Hut 102 9.18 x 105 4 0.39% 7.68 x 105 3 10.60%

Counting Chambers — No Washing or Contamination

Cellometer auto T4 Counting Chambers

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings — no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume
  • Large-depth chambers for large cells
  • Most affordable automated counting consumables

Easily Print & Save Data and Cell Images

Export data to Excel

Easily Export to Excel for additional data formatting or sharing. Images and data tables can be pasted into PowerPoint presentations or submitted for publication.

Save data

Easily Save data and images to a network or local computer.

Print data

Print directly from the software screen. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images.

Dedicated On-line and On-site Applications Support

Nexcelom Technical Support Specialists

Experienced Nexcelom Technical Support Specialists are available from 9am to 5pm EST for phone and on-line support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of a new Auto T4 Cell Counter

Help

The help button at the bottom right of the Cellometer Auto T4 software screen gives users instant access to:

  • Software features and instructions
  • On-line tutorials and training videos
  • Submission of a Support Ticket

Submit a Support Ticket

Clicking “Submit a Support Ticket” launches a convenient on-line form that is submitted directly to Nexcelom Technical Support

  • T4 instrument information fields are automatically populated
  • Images are easily attached for troubleshooting and applications support
  • A Nexcelom Specialist can assume remote control of the Auto T4 Cell Counter for troubleshooting and training

Nexcelom Field-based Applications Specialists

Nexcelom Field-based Applications Specialists are also available for:

  • On-site demonstrations
  • Training
  • Troubleshooting
  • Technical Seminars

All Nexcelom Applications Specialists are 100% focused on image-based cell concentration & viability and cell-based assays using Cellometer Image-Based Cytometry

Cellometer Auto T4 Accessories

IQ / OQ Validation Package

Installation Qualification (IQ) and Operational Qualification (OQ) package for the Auto T4 Cell Counter

Catalog # Description Size Unit
Cellometer Auto T4-IQOQ Counting chambers, reference bead solution, and validation protocol for the Cellometer Auto T4 100 counts 1 Set

On-Site Service, Maintenance, and Instrument Warranties

Trained Nexcelom Applications Specialists are available for on-site preventative maintenance, IQ validation, and OQ validation.

Service Description
Preventative Maintenance Available for all Cellometer Systems
Please inquire about scheduling and pricing.
IQ Validation* Available for Auto T4 and Vision Systems
Please inquire about scheduling and pricing.
OQ Validation* Available for Auto T4 and Vision Systems
Please inquire about scheduling and pricing.
Extended Warranty Available for all Cellometer Systems currently under a valid warranty.
Please inquire for pricing.
*IQ/OQ Validation Package must be purchased separately

Applications for Cellometer Auto T4 Bright Field Cell Counter

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »


Cell Image GalleryVisit theCellometer Cell Image Galleryfor a More Complete Listing

Cancer Cell Lines Counted with the Cellometer Auto T4

Cell Characteristics are based on observation in a Cellometer Counting Chamber. For adherent cell lines, cell characteristics are based on the appearance of cells following trypsinization.

786-O

786-0 cells

Origin: Kidney

Cell Characteristics: Round, normal size distribution

ACHN

ACHN cells

Origin: Kidney

Cell Characteristics: Mostly round, normal size distribution, clusters < 5 cells, debris

COLO 205

COLO 205 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution

HL-60

HL-60 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

HS 578T

HS 578T cells

Origin: Breast

Cell Characteristics: Large variation in morphology, debris

LOX IMVI

LOX IMVI cells

Origin: Skin

Cell Characteristics: Large variation in morphology, debris

MDA-MB-231/ATCC

MDA-MB-231/ATCC cells

Origin: Breast

Cell Characteristics: Round, some size variation, some debris

MOLT-4

MOLT-4 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

NCI-H23

NCI-H23 cells

Origin: Lung NSCLC

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

OVCAR-5

OVCAR-5 cells

Origin: Ovary

Cell Characteristics: Round, some size variation, clusters < 5 cells

RPMI 8226

RPMI 8226 cells

Origin: Leukemia

Cell Characteristics: Round, size variation

SF-539

SF-539 cells

Origin: Brain

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

SK-MEL-28

SK-MEL-28

Origin: Skin

Cell Characteristics: Variation in morphology and size

SNB-19

SNB-19 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SW620

SW620 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

A498

A498 cells

Origin: Kidney

Cell Characteristics: Round, some size variation

CAKI-1

CAKI-1 cells

Origin: Kidney

Cell Characteristics: Large variation in morphology

DU145

DU145 cells

Origin: Prostate

Cell Characteristics: Round, normal size distribution, clusters < 5 cells

HOP-62

HOP-62 cells

Origin: Lung, NSCLC

Cell Characteristics: Round cells, some size variation, cell clusters < 5 cells

HT-29

HT-29 cells

Origin: Intestine

Cell Characteristics: Round, normal size distribution, clusters < 5 cells

M-14

M-14 cells

Origin: Skin

Cell Characteristics: Round, some variation in size

MDA-MB-435

MDA-MB-435 cells

Origin: Breast

Cell Characteristics: Round, some size variation

NCI/ADR-RES

NCI/ADR-RES cells

Origin: Ovary

Cell Characteristics: Round, normal size distribution

NCI-H322M

NCI-H322M cells

Origin: Lung, NSCLC

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

OVCAR-8

OVCAR-8 cells

Origin: Ovary

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

SF-268

SF-268 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SK-MEL-2

SK-MEL-2 cells

Origin: Skin

Cell Characteristics: Large variation in morphology and size

SK-OV-3

SK-OV-3 cells

Origin: Ovary

Cell Characteristics: Variation in morphology and size, some clusters < 5 cells

SNB-75

SNB-75 cells

Origin: Brain

Cell Characteristics: Round, some variation in size and morphology

T-47D

T-47D cells

Origin: Breast

Cell Characteristics: Large variation in morphology and size, some clusters < 5 cells

A549/ATCC

A549/ATCC cells

Origin: Lung, NSCLC

Cell Characteristics: Round, normal size distribution, clusters > 5 cells

CCRF-CEM

CCRF-CEM cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

EKVX

EKVX cells

Origin: Leukemia

Cell Characteristics: Round cells, variable size distribution, clusters < 5 cells

HOP-92

HOP-92 cells

Origin: Lung: NSCLC

Cell Characteristics: Round cells, some size variation

K562

K562 cells

Origin: Leukemia

Cell Characteristics: Round, normal size distribution

MALME-3M

MALME-3M cells

Origin: Skin

Cell Characteristics: Round, normal size distribution

MDA-MB-468

MDA-MB-468 cells

Origin: Breast

Cell Characteristics: Round, normal size distribution, some clusters < 5 cells

NCI-H226

NCI-H226 cells

Origin: Lung, NSCLC

Cell Characteristics: Round, some size variation, some clusters < 5 cells

OVCAR-4

OVCAR-4 cells

Origin: Ovary

Cell Characteristics: Round, some size variation, clusters < 5 cells, some debris

PC-3

PC-3 cells

Origin: Prostate

Cell Characteristics: Round, normal size distribution

SF-295

SF-295 cells

Origin: Brain

Cell Characteristics: Round, some size variation, clusters < 5 cells

SK-MEL-5

SK-MEL-5 cells

Origin: Skin

Cell Characteristics: Variation in morphology and size

SN-12C

SN-12C cells

Origin: Kidney

Cell Characteristics: Round, normal size distribution

SR

SR cells

Origin: Lymphoma

Cell Characteristics: Large variation in morphology

UACC-257

UACC-257 cells

Origin: Skin

Cell Characteristics: Large variation in size and morphology, some clusters < 5 cells

Performance of the Cellometer Auto T4 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N=20 Hemacytometer Auto T4
Average 1.03E+06 1.08E+06
STDEV 6.60E+04 1.01E+05
%CV 6.4% 9.4%

5 µm beads

N=20 Hemacytometer Auto T4
Average 1.07E+06 1.03E+06
STDEV 5.90E+04 6.46E+04
%CV 5.5% 6.3%

Models and Specifications for Cellometer Auto T4 Bright Field Cell Counter

Includes • Cellometer Auto T4 Instrument
• Cellometer Software
• USB 2.0 Connection Cable
• Power Supply
• Phone/online applications support during set-up
Available Accessories PC laptop
Imaging Performance Cell Size: 5 – 60 microns (most mammalian cells)
Conc. Range: 105 – 107 cells/mlBright field imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
Instrument Specifications Weight: 10.4 lbs. (4.7kg)
Width: 3.5″ (8.9 cm)
Depth: 4.2″ (10.7 cm)
Height: 12.6″ (32.0 cm)Input to Power Adapter: 100-240 VAC, 50/60 Hz, 0.5A
Output to Instrument: 12 VDC, 1.5A
PC / Laptop requirements: (If purchasing Cellometer without PC laptop) • Windows 7, 8 or 10
• 1.6 GHz or higher processor
• 2GB RAM
• USB 2.0 port
• Display resolution: 1024 x 768 pixels or higherIntel Mac with Parallels/Windows

The Cellometer Auto T4 cell counter has made cell counting simpler and more standardized. A tedious job has been eliminated. Thanks Nexcelom!

John Sipes, NCI

Our Cellometer Auto T4 has continued to be a time saver and make cell counting less of a headache! Being able to program different cell types is also a great feature.

Colleen Worne, University of Utah

I really like the Cellometer Auto T4! It saves a lot of time and is very convenient for counting cells, requiring very little sample. It doesn’t take up much room either!

Laura Garcia, UPMC

The Cellometer Auto T4 cell counter has made cell counting simpler and more standardized. A tedious job has been eliminated. Thanks Nexcelom! – John Sipes, NCI

Our Cellometer Auto T4 has continued to be a time saver and make cell counting less of a headache! Being able to program different cell types is also a great feature. – Colleen Worne, University of Utah

The Cellometer Auto T4 is small and compact. The cell counts are fast, consistent and reliable. The T4 software is very user-friendly!

I like how fast the Cellometer Auto T4 is. You can see pictures of your cells and cell counts within seconds! It makes me confident in my cell counts when I am preparing an experiment.

The Cellometer Auto T4 is really awesome! I culture a lot of cell lines at one time so cell counting is time-consuming. This cell counter helps me a lot!

The Cellometer Auto T4 provides rapid counting of various cell sizes and cell viability.

I really like the Cellometer Auto T4! It saves a lot of time and is very convenient for counting cells, requiring very little sample. It doesn’t take up much room either! – Laura Garcia, UPMC

The Cellometer Auto T4 has changed the way I count cells and I couldn’t be happier about it. No more painstaking hand counting using the hemacytometer. Thanks so much Nexcelom! – Solomon C. Amadiume, Weill Cornell Medical College

Using the Cellometer Auto T4 has saved me so much time! I am now able to count many cell lines within just a few minutes! – Anita Robin, Weill Cornell Medical College

The Cellometer Auto T4 works very well! There is no lag when adjusting the mirror’s focus as well! Cell counts are done precisely and in a timely manner. – Andres Enrique Martinez-Muniz, University of Puerto Rico Mayaguez

As a new Research Technician in my lab, I found the Cellometer Auto T4 to be very user friendly. It counted my cells quickly and accurately, which is vital for my cell culture talks required of me weekly!

The Cellometer Auto T4 and the software are easy to use! Now it is very easy for me to set up experiments with different cell lines. Getting accurate cell count is so easy with the Auto T4. – Leticia Reyes Ramos, Medical University of South Carolina – Pharmacology

I have really enjoyed using the Cellometer Auto T4. It is superior for determining cell viability of immune cells.

We have two Nexcelom cell counters in our lab, a Cellometer Auto T4 and Cellometer Auto 2000. Both cell counters have saved us critical time and are very easy to use! The Cellometer Auto 2000 has been invaluable at helping up with difficult-to-count cells, and even with developing new assays. Both are indispensable pieces of equipment in our lab. After trying our Auto 2000, another lab in our building is purchasing one for their own use.

The Cellometer Auto T4 is certainly a lifesaver! I can get through my experiments much quicker, especially when I have numerous samples. It is also helpful when I have a very high cell concentration because I can trust that I am getting an accurate cell count without a lot of effort on my part.

I have used the Cellometer Auto T4 for many years, It is easy to use and I have never had a problem with it! It counts cells quickly and saves me a lot of time!

We have the Cellometer Auto T4, Cellometer Auto 2000 and Cellometer Vision CBA. Our Cellometers are reliable and every time I call for a product or technical service, I have spoken to a helpful and knowledgeable person.

The Cellometer Auto T4 has been very helpful for our mouse studies. It allows us to count cells to determine how many to use for our flow experiments.

The Cellometer Auto T4 from Nexcelom is a great machine to count cells. It is easy to use and very accurate in cell counting. Using this cell counter give more homogeneous results with different lab individuals. In addition, it has a vast range for counting cells so you do not need to dilute your sample again to be in the range of the cell counter. – Rohit Upadhyay, Tulane University

We have a Cellometer Auto T4. We like it so much we got spoiled and bought another one! No waiting to count! Everyone is happy and loves this machine!

Nexcelom’s Cellometer Auto T4 makes counting cells super easy! It is very easy to use and the programs are very user friendly. Plating cells is a super quick process now! – Connor Dyer, Stanley Manne Children’s Research Institute

The Cellometer Auto T4 has made counting cells so much faster and easier since we purchased the machine. Before acquiring the Cellometer, we had to count using the “old school” hemacytometer which would take a long time.

The Cellometer Auto T4 has transformed my cell counting! The time in the TC room has gone way down, and cell viability is always where I want it!

The Cellometer Auto T4 is easy to use and maintain, very user friendly and comes with excellent tech support! I’ve never had any issues with the machine malfunctioning or the software crashing. It is also very easy to import datasets from the software, making it easy to extract and analyze data. – Samantha Stanley, University of Houston

I like the Cellometer Auto T4 a lot! It’s so fast and convenient compared to the traditional way of counting. My favorite feature is that you can manually change the cell count numbers slightly if needed.

The Cellometer Auto T4 is fun, works reliably and fast. It is easy for new people to learn and gives us a break in the busy schedule. – Thao Nguyen, University of Houston, BME Department

The time savings are priceless with the Cellometer Auto T4. Everyone in the lab says it’s awesome! Wonderful customer service, too! We received thorough training from our Nexcelom rep.

We like the Cellometer Auto T4 for quick cell counts, viability assays, and importantly – we use it to monitor cell volume expansions of T-cells after stimulation. We get good cell diameter measurements, which can useful for volume calculations and more.

The Celigo is a great instrument for cell killing assays. Also, the Cellometer Auto T4 allows cell imaging and prevents having to use a hemacytometer which is very nice. This device is an excellent, much faster alternative to other counters. It has enables us to visualize tumor single cell suspensions!

I love that the Cellometer Auto T4 can count different cell sizes from a tumor sample! Goodbye hemacytometer!

The Cellometer Auto T4 has made cell counting an enjoyable, much faster experience. Plating cells has never been easier and more accurate!

The Cellometer Auto T4 is very convenient and consistent. Best product I’ve used in my academic career, and it sure beats the hemacytometer!

The Cellometer Auto T4 is very friendly and easy to use piece of equipment – it only took me a matter of minutes to learn how to use it. Also, it gave me accurate results in a short period of time which is perfect to perform experiments fast, and I am able to use it when a short period of time is required based on experiment.

The Cellometer Auto T4 is an essential work horse of our cell culture facility. The unit delivers consistent and reliable results, while being remarkably easy to use!

The Cellometer Auto T4 has expedited our vast tissue culture work with easy, customizable counting. The value cannot be understated! – Megan Conlon, Stanford

The Cellometer Auto T4 is very easy to use. It makes my day less stressful and the staining is quick an easy! The results are timely and help me get my counts in a timely manner.

The Cellometer Auto T4 is very easy to use. I like that I can set the type of cells being measured in the software. It gives and easy-to-use read out. I used the Countess previously and I prefer this system over it! – Amy Rymaszewski, Medical College of Wisconsin

On the Cellometer Auto T4, the saved parameters for different cell types enable me to count different cell types efficiently and without having to manually adjust the conditions for each one.

The Cellometer Auto T4 has been a great addition to our lab! It greatly reduces the amount of time needed to count cells and helps consistency between individuals. Overall, I am very pleased with the performance and ease of use! – Jesse Washnock-Schmid, University of Michigan

Using the Cellometer Auto T4 has cut down the amount of time spent on counting dramatically! We also tend to find more consistent cell counts than when using the hemacytometer.

Here at the University of South Florida, we use the Cellometer Auto T4 in our labs. Its precision in counting and the feature for showing counted and uncounted cells are my favorite features of this device. The counting process is fast and also easy to operate. I love using this machine! – Shihab Uddin, University of South Florida

The Cellometer Auto T4 has drastically improved my efficiency in the lab. The speed and precision with which it counts cells has saved me so much time! It is very user friendly and I would highly recommend it to anyone who does any kind of cell culture work.

The Cellometer Auto T4 is very easy to use, reliable and fast! No specialized skill is needed. I have used this instrument for 7 years and never have any problems with it!

I use the Cellometer Auto T4 for counting primary cells containing heterogeneous populations of cells. It has been a great help since primary cells contain so many undetectable cells using mechanical cell counting procedure. This allows me to detect the cell sizes of my own interest, which is cancer cells.

The Cellometer Auto T4 has helped me save time in my research, improved reproducibility in my cell counts and allowed us to do more counts in each time point. I cannot think about doing research without it! – Fernando Merida, University of Puerto Rico, Mayaguez Campus

The Cellometer Auto T4 is convenient to count cells and results are consistent when we compare with a hemacytometer. The instrument automatically excludes Trypan Blue stained dead cells.

The Cellometer Auto T4 is a precise and flexible tool for cell counting. Data recording and customizable tools are very convenient.

The analysts in the lab really like working with the Cellometer Auto T4 due to rapid results and ease of use! It has good accuracy and the images are easy to read.

The Cellometer Auto T4 is very simple and user friendly! It’s especially useful to be able to save different cell lines, so that the reading can be more accurate for different cell lines.

We have the Cellometer Auto T4 system, and it is very easy to use! We also had the chance to meet with our local rep and learn more about the Celigo Imaging Cytometer. We are looking forward to learning about the system and using it! – Mania Kavosi, Pfizer

The Cellometer Auto T4 is a great instrument to simultaneously calculate cell concentration and percent viability for different kinds of cultured cells stained with Trypan Blue. We have been using regular glass hemacytometer with a coverslip, counting under the microscope for hours and clean it with alcohol before repeating. This instrument is a time saver, period! Live to dead discrimination is perfect for most cultured cancer cell lines. I have not found any difficulties counting with logical cell culture density. I recommend this product for everyone who is looking for a fast and reliable cell culture counting instrument! The software is very easy to use, and results are few clicks away.

On the Cellometer Auto T4 I appreciate the automated data recording/log-ins. It has saved a lot of time! The ability to save the images and reanalyze them later is also very useful for reviewing and auditing old data!

I love this machine! The Cellometer Auto T4 is very convenient to use and super helpful to our culture work. It needs only 20uL cell suspension and takes 1 minute to count and you are all set! – Qianqian Guo, Mayo Clinic

The Cellometer Auto T4 makes it easier to count the cell numbers so we can save time, especially when having a lot of samples! It also increases the accuracy of our cell count compared to using manual methods.

The Cellometer Auto T4 is very helpful as we are able to distinguish the live from the dead cells. I would definietely recommend it! It also has good quality images that makes work easier!

The Cellometer Auto T4 has allowed our lab to do more accurate cell experiments by helping us quickly and easily count our cells. I like the easy-to-use interface of the counting software which allows us to use any cell type and count quickly! – Sheri Kelemen, Temple University

I am using the Cellometer Auto T4 as well as another cell counter. I stain with Nexcelom’s AOPI when I use the other cell counter and it works great! I have the perfect cell stain. I love it!

The Cellometer Auto T4 provides very quick cell counts and viability checks.

The Cellometer Auto T4 is a very reliable device, we use it to count various cell types everyday! The software to drive the Auto T4 already pre-installed some types of cells for you, such as human and mouse cells, and many cell lines as well.

The Cellometer Auto T4 is very useful when I have to deal with more than 4-6 samples. Based on some comparison tests with the classic hemacytometer, the machine is quite accurate!

This Cellometer Auto T4 is fundamental to a lot of the work done in my lab. It can be relied upon to give precise cell counts in a matter of seconds, and helps save a lot of time in the lab! – Ryan Brown, University of Chicago

The Cellometer Auto T4 is a huge help in performing cell seeding and drug treatment as well as other cell assay related experiments in the lab. The Auto T4 allows us to count the cells very fast and set the cell size parameters. The disposable sample chambers prevent contamination and are easy to use. It also offers the ability to save the data and print it.

The Cellometer Auto T4 and Cellometer K2 make it really easy to obtain cell numbers and makes counting cells less of a task! Basically makes life easy! – Aparna Swarup, Wuxi AppTec

The Cellometer Auto T4 is very intuitive and works great! Overall, the best cell counter that I have used! – Rick Huang, University of Colorado, Anschutz Medical Campus

I use the Cellometer Auto T4 very often and the Celigo Imaging Cytometer occasionally. Having an automated cell counter has made cell counting much faster! The viability determination is an added bonus. Besides the library of cell types, the ability to create a new cell type is easy. The few times I have contacted customer service, they have always been helpful and the replies were fairly quick!

The Cellometer Auto T4 is much more convenient than using a hemacytometer. Also, I like how there are many different cell types to chose from!

I’ve used the Cellometer Auto T4 for many cell lines and I like it because there are so many options for cell types.

The Cellometer Auto T4 is a very easy and reliable technique that we are using for cell viability assays. It’s very easy to handle the instrument, and the sample consumption is low and you can take as many replicates as you want. The software is flexible and gives you a variety of options, such as set the range of diameter of the cells and use the calculator to find the volume needed for desired number of cells based on the cell number you got. The slides are comfortable and can perform two counts on each one. Sample loading is simple and easy to count and you can also save the data generated.

My lab loves the Cellometer Auto T4! We use it multiple times each day. It is easy to focus and see the counts. We love how we can manually make sure the counter is working correctly and adjust it the few times it may miss some cells. Plus, the fact that you can import cell types from the pre-loaded bank makes it easy to count new cell lines. – Luis Orozco, SBP Disovery Institute (CPCCG)

The Nexcelom Cellometers are the way to go! They are easy to use and give you cell counts fast. The software for the Cellometer Auto T4 is easy and intuitive. But most importantly, the consumables are very cost-effective relative to competitor products.

The Cellometer Auto T4 is a nice device for cell counting! The great feature is the ability to count clumps of cells! All counted cells are highlighted by green and the dead cells by red. – Artem Kononenko, Tufts University

The Cellometer Auto T4 is a very high functioning machine that operates with high accuracy and precision for measuring concentrations and percent viability for cultured cells stained with Trypan Blue. The Auto T4 meets the claims of reporting within 30 seconds the live, dead, and total cell count, the live and total cell concentration; the mean cell diameter, and the percent viability. I really love the ability and functions where it automatically generates a cell size histogram based on cell size. I love this machine and it is a must-have for our upstream lab!

The Cellometer Auto T4 is very useful to count low numbers of cells and also to check viability. It is quick and easy to use, I would refer it to other colleagues! – Elizabeth Graver, MD Anderson Cancer Center

The Cellometer Auto T4 automated cell counter makes the lab life fun and easy! Before this, I used a hemacytometer for counting cells which took so much time and had many difficulties, especially when the number of samples were large. The Auto T4 is able to ease that pain. Also, its precision is very high and numbers are very reproducible. Overall A+ – Wishrawana Sarathi Ratnayake, University of South Florida

The Cellometer Auto T4 has led to precise cell counts quickly and easily which has revolutionized my workflow and substantially cut down on time in the lab!

The Cellometer Auto T4 has made counting much easier and efficient. It saves so much time and yields precise results. – Tracess Smalley, University of South Florida

The Cellometer Auto T4 saves us time and helps us to minimize subjective judgement. At the same time, the Cellometer provides an excellent way to store the results to present and discuss in groups when needed.

Our Cellometer Auto T4 is easy to use, quick, and provides comprehensive information about our cells, such as cell counts for alive and dead cells. Having used a manual hemacytometer in the past, I am very pleased with the Cellometer Auto T4.

We use the Cellometer Auto T4 for counting leukocytes in single cell suspensions of mouse skin. This machine is a great addition to our lab, as leukocytes are too small of a population to manually count via hemacytometer. The counts are quick, reliable, and have sped up our isolation process for a number of applications. We are very satisfied with this product. -Michael Podolsky, Penn State University

We rely on our Cellometer Auto T4 to count for all the cell counting in the lab! The calculator function is a great tool to have.

The Cellometer Auto T4 unit simplifies the counting of a variety of cell types and makes a more consistent counting method, which equals better science and confidence in our data. The system is easy to use and the operation for cell counting optimization and calculations is a powerful aspect of the system.

The Cellometer Auto T4 has been a great addition to the lab. It is extremely accurate at cell counting in addition to detecting live and dead cells by Trypan Blue staining. The flexibility of reading cells of various sizes makes this optimal for any laboratory. The software is fast and easy to use!

The Cellometer Auto T4 has been an absolute dream! It seems almost too easy to count live cells, compared to the hemacytometer that I was accustomed to. I can now quickly count cells and use the program to estimate volumes of cells that are needed in order to carryout my experiments. I also get clean images of cells to assess their quality and fitness.

The Cellometer Auto T4 is very user friendly! We use it mainly for cell count and viablility with our primary T cells. It is vey convenient and fast!

Starting-up a new lab space in 2014 at a predominantly undergraduate teaching university, I was a professor at research-intensive universities for many years, but had had little prior experience with wide-eyed undergrad students. I was re-defining myself as a teacher in the process and had to figure out how to offer these young people an inspirational research course. One constraint was the time we usually have them (often just one semester) meant only simplest things, which inevitably meant grunt work, which too often I felt resulted in non-inspired students. I knew they intuitively gravitated to tissue culture experiments, but mostly these are too difficult to learn without contaminating the whole lab, and the data output one gets from tissue culture is so often of a molecular nature that its hard to get any reliable results in but one semester exposure to research. But, in discovering Nexelcom’s Auto-T4 cell counter, I felt that cell morphometry offered an ideal first step. These instruments admirably are now helping most of my students get off the ground in their first research experiences intact with a good feeling about science. I still guide them carefully to the point of cell harvesting, but then they manage the centrifuges, pipettors, staining and then comes using the Cellometers – and they love the Cellometers! Throughout these exercises they invariably endure novice mistakes and have to red-do over and over, but it isn’t too too hard for them to acquire data with the Auto-T4 – and its safe and not overly expensive – and thus its worked well for my needs. Then, once they got the hang of this high-tech machine, their feet are wet and many of them spur onward. Next, I like to see a sparkle in their eyes and they actually want me to give them a rigorous boot camp lab experience. For those that I accept into my “lab boot camp,” it has been great and we’re now able to publish papers! Thinking back on how we got this far, it seems they all were initiated via Nexelcom’s AutoT4 instrument; mainly because it it so user-friendly and generates such a variety of data quickly. At the same time, the Auto-T4 has been a workhorse for my more formal graduate students. It stands as an integral tool we use at Mississippi College to create a great research elective expereince. Once that first fish is caught, it inspires them to want to spend more time and energy to learn many other techniques and little by little some of them get the research bug. My hope is that all this challenges their minds, eventually changes some lives, fosters careers in science, and advances a small part of God’s kingdom. – John Piletz, Mississippi College

Our lab is using the Cellometer Auto T4, and this is the best cell counter I have ever used. You will be able to reach the cell numbers and viability at the same time. You can also set up your cell type to get more precise counting. It is a very good instrument.

The new version of the Cellometer Auto T4 counts very fast. We have used the Auto T4 for almost 10 years.

We have the Cellometer Auto T4. It has greatly accelerated the rate of production in our tissue culture facility. It’s very accurate and we get reproducible results each time! -Jeff Anderson, Northwestern University

We like to use our Cellometer Auto T4 on chicken hepatocytes! -Adrian Bustamante, Ceva Biomune

I have used three different models on the Cellometer automated cell counter platforms. The all-in-one is easy to use. The Cellometer Auto T4 is the best cell counter by far and works exceptionally well with fast computers. This beats manual cell counting hands down!

I have been using the disposable cell counting chambers with the Cellometer Auto T4. Another lab had purchased this unit a few years ago and I just started using the Auto T4 last year. It saves a lot of time and is very convenient for counting cells, requiring very little sample. It doesn’t take up much room either!

Our Cellometer Auto T4 enables us to provide accurate and reproducible cell counts and viability assessments very quickly. An accurate cell count is crucial to our research and the automated system of the Cellometer reduces intra- and inter-measurement error. –Gareth Willis, Boston Children’s Hospital

 I have a Cellometer Auto T4. It’s an excellent device that is easy to use and provides very accurate and consistent cell counts. Compared with other devices, it saves my lab a lot of time and avoids messes in the cell culture room. Highly recommended. –Roderick O’Sullivan, University of Pittsburgh

The Cellometer gives a quick and easy way to count individual cells and also increases the throughput and efficiency compared to manual counting. I am working with 200 different clones that needed to be counted and I am able to get the cell count, concentration and %viability in a fairly short period of time! If I had not used the Cellometer Auto T4 cell counter, I would most likely not have been able to finish counting cells from each clone. Manual counting would have been extremely time-consuming and I would be particularly concerned with miscount errors that affect the accuracy of the cell concentrations. All in all, this instrument has definitely been a life saver for me!