Cellaca® PLX Image Cytometry System

Multiplexity Without Complexity

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Immunophenotyping, Cell Counts, and Viability Readouts in Seconds

The Cellaca PLX system with Matrix analysis software and dedicated reagents and consumables provides a benchtop solution for accurate measurements of small sample volumes to easily perform rapid subpopulation analysis for downstream processing.

Multiplexing Made Easy
The Cellaca PLX image cytometry system is easy to use, performing simple yet sensitive cell counts, viability readouts, and multiplex analysis in seconds.

Small Sample Volume
The sample-efficient Cellaca PLX requires between 15 – 50 uL per single sample analysis. That’s 10-30 times less sample volume required per test, compared to a flow cytometer.

Speed with Sensitivity
The time to downstream processing is shortened by multiplexing with four channels with viability readouts at one minute per sample.

Optimized Assays and Kits
Our simple-to-use, mix, incubate, wash, and read reagent kits provide immunophenotyping plus viability multiplexing assays using disposable, low fluorescence consumables.

Stress-Free Software
Optimized protocols streamline surface marker staining, vitality, and apoptosis analysis with step-by-step methodologies and customizable result presentations.

Low Auto-Fluorescent Consumables
Allow for accurate surface marker detection.

Hardware, Instruments, and System Capabilities

The Cellaca PLX’s ability to consistently image, de-cluster, and report cell counts, viability, concentration, and phenotyping data makes it a versatile instrument during every stage of cell line development. The Cellaca PLX allows labs to shorten the time to downstream processing by multiplexing with up to four channels to obtain critical viability readouts at one minute per sample with no warm-up time. The cell population data can then be viewed as a histogram, scatter plot, dot plot, or contour plot for easy data viewing and analysis.

Whether using it as a complementary method for additional data or as the first stop before starting downstream assays, image cytometry can save laboratories time and prevent excessive sample loss. With this output speed, researchers can quickly perform cell purity checks at the bench and then easily continue with downstream assays without waiting for a flow cytometer. By performing multiplexing assays at the bench, laboratories can reduce the reliance on a busy flow core facility, saving flow cytometry for more complicated or detailed assays.

Expert Support for Assay Development

On-site, remote, and hybrid training are available to assist with initial setup and continued assay support for improved workflows.

Multi-Language Support

Offering over 7,000 language settings for easy global data transfer.

Remote and Field Serviceability

Calibration-free instrumentation with minimal to no routine maintenance. No clogging or liquid waste stream.

Automation Ready

Compatible with robotic integration and automated liquid handling systems.

21 CFR Part 11 Ready

  • Audit trails, time stamping, user access control, and electronic signatures.
  • File locking and change tracking.
  • Creation of new customizable defined roles with multiple configurations available
  • A close-looped database system to aid with data integrity and security.

Stress-Free Intuitive Software

  • Built-in assays with optimized settings for over 400 individual cell types.
  • Customizable data and calculation reports with graphs, images, charts, and tables for ease of information sharing.

The multi-color results image provides a visual of the various overlayed channels differentiating the cells while the cell count and viability data are captured below.

Cellaca MX cell types

Low Auto-Fluorescent Consumables

Cellaca PLX consumable slides provide a safe and self-contained environment for the sample which then does not require a decontamination procedure of the instrument at the end of each run. The slide holder, which is included with the instrument, can accommodate 4 slides or 8 samples per experimental run.

Cellaca Product Line

The Cellaca instrument line is comprised of the Cellaca MX high-throughput cell counter with brightfield and two fluorescent channels, and the Cellaca PLX image cytometry system with brightfield and five fluorescent channels for highly accurate multi-channel analysis.

The Cellaca PLX is the only multi-channel analysis instrument enabling rapid cell count, concentration, immunophenotyping, and viability readouts to significantly improve the time to downstream processing.

Cellaca PLX Cellaca MX FL2
Channels Brightfield, Blue, Green, Orange, Red, Far Red Brightfield, Green, Red
Number of Fluorescent Channels 13 (6 per scan) 3 (2 per scan)
Excitation LED 370, 475, 531, 628 nm 470, 527 nm
Emissions Filters 452, 534, 605, 655, 692 nm 534, 655 nm
Commonly Used Compatible Dyes and Assays Trypan Blue, AO/PI, Hoechst, DAPI, GFP, RFP, CMFDA, Calcein AM, 7AAD, Annexin V, PE, APC, Kiravia Blue 520 Trypan Blue, AO/PI, Calcein AM, Annexin V, Caspase 3/7
Counting Speed per well Fluorescence 4-channel scan – Less than 1 minute Trypan Blue – 2 seconds

Fluorescence 2-channel scan – Less than 8 seconds

Volume (per well) 15 µL in slides 50 µL – 200 µL in counting plates 50 µL – 200 µL in counting plates
Size/Diameter Range 5 – 80 µm 5 – 80 µm
Concentration Range 1×105 – 1×107 1×105 – 1×107
IQ/OQ Option Yes Yes

Associate Director of the Flow Cytometry facility at The Broad Institute, Patricia Rogers, has worked with a wide range of instrumentation and vendors in her fifteen plus years in Flow Cytometry. Patricia knows the value of great customer service and support and she believes that great instrumentation must go hand-in-hand with rapid reacting customer service to truly be exceptional. Luckily, we here at Nexcelom value the same principles and we are proud to offer excellent customer service and support to all our customers.

Watch the video to see what she says about the Cellaca MX Cell Counter.

“Anytime I have a problem you guys are always there to help me figure out a solution so you know, the service has really been more like a partnership and I’m really happy with it.”

Patricia Rogers, Associate Director of the Flow Cytometry facility, The Broad Institute

The Cellaca PLX is Suitable for a Variety of Cell-Based Assays

Optimized Assay Reagents and Kits

The Cellaca PLX provides flow-like data with simple-to-use, mix, incubate, wash, and read reagent kits that provide immunophenotyping plus cell counts and viability multiplexing assays using disposable, low fluorescence consumables. Quickly perform cell counts, concentration, purity checks, and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.

Available assay kits include:

  • Immune Cell Phenotyping
  • Apoptosis Detection
  • High-Throughput Cell Viability
  • Fluorescent Protein Analysis

 

Product Name Green Red Far Red Blue Part No. (25 tests)
1 Surface Marker
for Cell Types: PBMC, PBMC-pure, cell lines
KIRAVIA Blue 520 anti-human CD3 Antibody* CD3 CS1-A0001-1 (25 tests)
CS1-A0001-2 (100 tests)
Cellaca® PLX, anti-human CD3 PE Antibody CD3 CS1-A0002-1 (25 tests)
CS1-A0002-2 (100 tests)
Cellaca® PLX, anti-human CD3 APC Antibody CD3 CS1-A0003-1 (25 tests)
CS1-A0003-2 (100 tests)
Cellaca® PLX, anti-human CD4 PE Antibody CD4 CS1-A0008-1 (25 tests)
CS1-A0008-2 (100 tests)
Cellaca® PLX, anti-human CD4 APC Antibody CD4 CS1-A0009-2 (100 tests)
Cellaca® PLX, anti-human CD8 PE Antibody CD8 CS1-A0012-1 (25 tests)
CS1-A0012-2 (100 tests)
Cellaca® PLX, anti-human CD8 APC Antibody CD8 CS1-A0014-1 (25 tests)
CS1-A0014-2 (100 tests)
Isotype only
for Cell Types: PBMC, PBMC-pure, cell lines
KIRAVIA Blue 520™ Mouse IgG1, κ Isotype Ctrl Antibody* IgG1 CS1-A0004-1 (25 tests)
CS1-A0004-2 (100 tests)
Cellaca® PLX, PE Mouse IgG2a, κ Isotype Ctrl Antibody IgG2a CS1-A0005-1 (100 tests)
Cellaca® PLX, APC Mouse IgG2a, κ Isotype Ctrl Antibody IgG2a CS1-A0006-1 (100 tests)
Cellaca® PLX, PE Mouse IgG1, κ Isotype Ctrl (FC) Antibody IgG1 CS1-A0013-1 (25 tests)
CS1-A0013-2 (100 tests)
Cellaca® PLX, APC Mouse IgG1, κ Isotype Ctrl Antibody IgG1 CS1-A0011-1 (100 tests)
1 Surface Markers + Viability + Isotype Controls
for Cell Types: PBMC, PBMC-pure, cell lines
Cellaca® PLX, anti-human CD3 KB520 Viability Kit CD3 Dead Total CSK-A0001-1 (25 tests)
CSK-A0001-2 (100 tests)
Cellaca® PLX, anti-human CD3 PE Viability Kit CD3 Dead Total CSK-A0002-1 (25 tests)
CSK-A0002-2 (100 tests)
Cellaca® PLX, anti-human CD3 APC Viability Kit Dead CD3 Total CSK-A0003-1 (25 tests)
CSK-A0003-2 (100 tests)
Cellaca® PLX, anti-human CD4 PE Viability Kit CD4 Dead Total CSK-A0004-1 (25 tests)
CSK-A0004-2 (100 tests)
Cellaca® PLX, anti-human CD4 APC Viability Kit Dead CD4 Total CSK-A0005-2 (100 tests)
Cellaca® PLX, anti-human CD8 PE Viability Kit CD8 Dead Total CSK-A0006-1 (25 tests)
CSK-A0006-2 (100 tests)
Cellaca® PLX, anti-human CD8 APC Viability Kit Dead CD8 Total CSK-A0007-1 (25 tests)
CSK-A0007-2 (100 tests)
2 Surface Markers + Total + Isotype Controls
for Cell Types: PBMCs
Cellaca® PLX, anti-human CD3 KB520/CD4 APC Total Cell Kit CD3 CD4 Total CSK-A0008-2 (100 tests)
Cellaca® PLX, anti-human CD3 KB520/CD8 APC Total Cell Kit CD3 CD8 Total CSK-A0009-1 (25 tests)
CSK-A0009-2 (100 tests)
Cellaca® PLX, anti-human CD3 APC/CD4 PE Total Cell Kit CD4 CD3 Total CSK-A0012-1 (25 tests)
CSK-A0012-2 (100 tests)
Cellaca® PLX, anti-human CD3 APC/CD8 PE Total Cell Kit CD8 CD3 Total CSK-A0013-1 (25 tests)
CSK-A0013-2 (100 tests)
Cellaca® PLX, anti-human CD4 PE/CD8 APC Total Cell Kit CD4 CD8 Total CSK-A0014-1 (25 tests)
CSK-A0014-2 (100 tests)
2 Surface Markers + Dead + Isotype Controls
for Cell Types: PBMC-pure, cell lines
Cellaca® PLX, anti-human CD3 KB520/CD4 APC Total Cell Kit CD3 CD4 Total CSK-A0016-2 (100 tests)
Cellaca® PLX, anti-human CD3 KB520/CD8 APC Total Cell Kit CD3 CD8 Total CSK-A0017-1 (25 tests)
CSK-A0017-2 (100 tests)
Cellaca® PLX, anti-human CD3 APC/CD4 PE Total Cell Kit CD4 CD3 Total CSK-A0020-1 (25 tests)
CSK-A0020-2 (100 tests)
Cellaca® PLX, anti-human CD3 APC/CD8 PE Total Cell Kit CD8 CD3 Total CSK-A0021-1 (25 tests)
CSK-A0021- 2 (100 tests)
Cellaca® PLX, anti-human CD4 PE/CD8 APC Total Cell Kit CD4 CD8 Total CSK-A0022-1 (25 tests)
CSK-A0022-2 (100 tests)
2 Surface Markers + Viability+ Isotype Controls
for Cell Types: PBMC
Cellaca® PLX, anti-human CD3 KB520/CD4 PE/CD8 APC Total Cell Kit CD3 CD4 Dead Total CSK-A0024-1 (25 tests)
CSK-A0024-2 (100 tests)
Cellaca® PLX, anti-human CD3 KB520/CD4 PE/CD8 APC Dead Cell Kit CD3 CD8 Dead Total CSK-A0025-1 (25 tests)
CSK-A0025-2 (100 tests)
3 Surface Markers + Total or Dead + Isotype Controls
for Cell Types: PBMC (Total Cell Kit Only)
for Cell Types: PBMC-pure, cell lines (Dead Cell Kit Only)
Cellaca® PLX, anti-human CD3 KB520/CD4 PE/CD8 APC Total Cell Kit CD3 CD4 CD8 Total CSK-A0026-1 (25 tests)
CSK-A0026-2 (100 tests)
Cellaca® PLX, anti-human CD3 KB520/CD4 PE/CD8 APC Dead Cell Kit CD3 CD4 CD8 Total CSK-A0027-1 (25 tests)
CSK-A0027-2 (100 tests)
For Apoptosis
for Cell Types: cell lines
Cellaca® PLX, Caspase 3/RubyDead Apoptosis Kit Casp Dead Total CSK-A0028-2 (100 tests)
Cellaca® PLX, Annexin V-FITC/PI Apoptosis Kit AV PI Total CSK-A0029-1 (25 tests)
CSK-A0029-2 (100 tests)
For Fluorescent Proteins
for Cell Types: cell lines
Cellaca® PLX, Hoechst/RubyDead Viability Kit Dead Total CSK-A0030-1 (25 tests)
CSK-A0030-2 (100 tests)
Slides
for Cell Types: PBMC, PBMC-pure, cell lines
Cellaca® PLX, Low Fluorescence Slides CHM2-ACR-001 (25 tests)
CHM2-ACR-002 (100 tests)


*KIRAVIA Blue 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation.

Associate Director of the Flow Cytometry facility at The Broad Institute, Patricia Rogers, has worked with a wide range of instrumentation and vendors in her fifteen plus years in Flow Cytometry. Patricia knows the value of great customer service and support and she believes that great instrumentation must go hand-in-hand with rapid reacting customer service to truly be exceptional. Luckily, we here at Nexcelom value the same principles and we are proud to offer excellent customer service and support to all our customers.

Watch the video to see what she says about the Cellaca MX Cell Counter.

“Anytime I have a problem you guys are always there to help me figure out a solution so you know, the service has really been more like a partnership and I’m really happy with it.”

Patricia Rogers, Associate Director of the Flow Cytometry facility, The Broad Institute

The Cellaca PLX Applications

Eliminate Complex Procedures and Precious Sample Loss

With the Cellaca PLX Platform, you can:

  • Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.
  • Stain transfected cell lines with viability dyes to determine the percentage of transfected or transduced cells that are live/dead.
  • Perform routine viability measurements as well as apoptosis functional assays to determine cell health.

 

Immunophenotyping Application:

Routine CD3, CD4, and CD8 population analysis is a standard practice in cell and gene therapy research. Understanding specific cell populations and viability allows researchers to move tested samples to subsequent downstream assays.

A. Brightfield

B. Hoechst

C. CD3-KIRAVIA Blue 520

D. CD4-PE

E. CD8-APC

Surface marker stained PBMC sample

PBMCs were stained with CD3, CD4, and CD8 surface markers, along with the total dye Hoechst. Image data (A-E) from the Cellaca PLX was exported to FCS Express for analysis. Gated nucleated (Hoechst +) CD3+ cells (~70% of total cells) are shown in the first scatter plot (F). The CD3 positive population was then further gated for CD4+ (~62%) and CD8+ (~28%) positive cells (G).

F. Hoechst+, CD3+

G. CD4+, CD8+

Apoptosis Application:

Apoptosis, viability, and other cell health indicators are vital in examining the state of collected patient samples. When sample viability drops below the set benchmark researchers often conduct multiple assays, including apoptosis, to understand possible causes for cell viability decline.

Fluorescent Protein Expression Application:

Transduction and transfection assays are often performed not only in cell and gene therapy but also in cell line development, viral vaccines, and multiple other areas of research. Quantifying the expression of fluorescent proteins, such as GFP and RFP, and the viability of those cells, is essential in determining whether gene insertion was a success.

Cellaca MX 2.0 Software