Celigo Imaging Cytometer

Microwell plate based image cytometer for adherent and suspension cells

See how the Celigo Imaging Cytometer works »
Celigo Plate Scan Speed

Celigo plate scan speed

Celigo Key Features

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Introduction to Celigo

Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system designed for live cell analysis.

Data Visualization

Convenient data visualization environment that facilitates data interpretation.


Automated microplate handling for either kinetic end-point analysis or time-point analysis.

Bright Field Imaging for All Well Sizes

The Celigo Image Cytometer provides the best-in-class bright field imaging for all well sizes.

API & Data Management

Application programing interface (API) and data management solution for integration into automated workflows.

Bright Field & 4 Fluorescent Channels

Bright field and four fluorescent channels (UV to Far Red) for analyzing & quantifying multiplex assays.

Customized Applications

Live cell analysis is performed using a series of customized applications for each assay – ready-to-use, does not require any image analysis expertise.

Select Science Interview

Automating Cell Imaging & Counting with Celigo

Select Science interview with Jean Qiu

The Celigo Cell Cytometer Counts Every Cell in Every Well

Celigo Cell Cytometer

Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system designed for live cell analysis. The best-in-class bright field imaging capability, in combination with 4 fluorescence channels, provide high speed, fully automated imaging and quantification of suspension, adherence cells, tumor spheroids, iPSC and cancer stem cell colonies within 6, 12, 24, 48, 96, 384, 1536-well plates, T flasks, and slides.

Proprietary optics and scanning system enable fast imaging of entire well while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.

With an intuitive software interface and optional integration into automation platforms, the Celigo provides labs with increased capabilities.

Celigo example images

The Celigo Image Cytometer Provides the Best-in-Class Brightfield Imaging for All Well Sizes

Excellent Optics for Enhanced Image Quality

Improve brightfield optical image quality at the edge of wells and eliminate edge optical distortion using an F-Theta lens for superior well edge-to-edge image contrast.

bright field cell imaging

Brightfield image taken with Celigo Imaging Cytometer at the edge of a well on a 96-well microplate showing enhanced image quality and contrast.

bright field cell imaging

Brightfield image taken with conventional microscope showing distortion and low contrast near the edge of the field-of-view.

Example of 12-well plate acquisition

Example of 12-well plate acquisition using Celigo Imaging Cytometer.

Example of 96-well plate acquisition

Example of 96-well plate acquisition using Celigo Imaging Cytometer.

Fast Plate Scanning for Image Acquisition and Analysis

Increase the speed of plate acquisition dramatically using novel plate scanning technology for fast acquisition. Scan a 384-well plate in brightfield in 2 min.

Ability to Scan a Variety of Plate Vessels

Scan wells of any size using automated image stitching that can view and quantify cells and colonies in vessels up to 6-well plates and 10 cm dishes.

Brightfield image of a well on a 96-well microplate

Brightfield image of a well on a 96-well microplate showing counted cell colonies across the well.

Accurately Quantify Cells and Colonies

Accurately quantify all the cells and colonies in the well even if they do not grow uniformly across the wells.

Brightfield image 96-well microplate showing counted adherent cells.

Brightfield image of a well on a 96-well microplate showing counted adherent cells.

Accurately Measure Adherent Cells without Trypsinization

Analyze your cell sample without trypsinization to avoid losing cells and look at cells right where they grow over multiple scan times.

Brightfield and Four Fluorescent Channels (UV to Far Red) for Analyzing & Quantifying Multiplex Assays

Fluorescent images acquired with Celigo Imaging Cytometer

Fluorescent images acquired with Celigo Imaging Cytometer of (a) Tubulin, Phalloidin, and DAPI, (b) Phospho-ERK and Hoechst, and (c) GFP and mCherry.

Brightfield and Multi-Channel Fluorescence Imaging

Use a combination of brightfield and fluorescent imaging allowing the development of multi-color assays ranging from UV to Far Red.

Flow-like data analysis

Flow-like data analysis and presentation generated by Celigo Imaging Cytometer. Fluorescence intensity (a) histogram and (b) scatter plot.

Powerful Image Analysis


Identify cell populations based on a variety of parameters such as morphology measurements or fluorescence intensities using a flow-like gating interface with histogram and scatter-plots graphics.

Correlation of gated scatter plot data to counted cells on acquired images

Correlation of gated scatter plot data to counted cells on acquired images.

Clear Visualization of Image and Data Correlation

Correlate the cell populations identified in gates with the cells visualized in the image using color coding overlays.

Convenient Data Visualization Environment that Facilitates Data Interpretation

Simple At-a-Glance Plate-Based Data Review

Quickly evaluate data from well to well using an at-a-glance view for each plate

384-well plate view of wells showing cell confluency

384-well plate view of wells showing cell confluency on the plate.

Simple At-a-Glance Plate-Based Growth Curves

Generate time course growth curves and display them in a plate overview for at-a-glance data review.

Time-course data plot generated by Celigo

Time-course data plot generated by Celigo Imaging Cytometer.

Easy Brightfield and Fluorescent Image Navigation

Navigate between plates, wells and time points to look at your cells growing in specific well locations.

Brightfield image of a single cell colony

Brightfield image of a single cell colony observed at the same location of a well on a 96-well plate.

Application Programing Interface (API) and Data Management Solution for Integration into Automated Workflows

Integrate with Robotics

Automate your assays by running the Celigo under the control of a scheduling software and integrate with robotic arms, plate stackers, automated incubators and liquid handlers.

Automated System for High Throughput Integrated Data Acquisition and Analysis

Acquire images and data analysis of hundreds of plates 24/7 automatically.

Flexible Data Analysis Method for Multiple Users

Analyze large data sets off the Celigo instrument using the Celigo Satellite Workstation and free up Celigo time to acquire more plates.

Easily Access and Manage Data using Celigo Network Database

Facilitate access to your imaging data using the Celigo network database and connect multiple instruments and satellite workstations to seamlessly acquire and analyze your data from a convenient centralized location.

Series of Customized Applications for Each Assay – Ready to Use, Does Not Require Any Image Analysis Expertise

Label-Free Brightfield Cell Analysis

Take advantage of label-free brightfield applications to avoid staining cells with toxic dyes or transfecting with fluorescent reporters.
brightfield cell counting, cell growth curve

Label-free brightfield cell counting and from (a) low count to (b) high cell count which can be used to directly generate (c) a cell growth curve

Numerous Cell Characterization Assays

Live cell analysis of images for cell counting, confluence, colonies and 3D-spheroids.

spheroids, cell counting, confluence percentage

Label-free brightfield cell counting of (a) spheroids, (b) cell counting, or (c) confluence percentage.

Ability to Export Brightfield and Fluorescent Images for Publications

Capitalize on the Celigo brightfield and fluorescent image quality to take images of your cells for your records and strengthen your publications.

Time-Saving Data Processing Method

Get data on the fly by acquiring and analyzing image simultaneously.

Easy-to-Use Preset Assay Parameters for Quick Data Analysis

Perform Celigo assays using pre-defined parameters that require very few modifications from the researchers.

Simple and Intuitive Software User Interface

Benefit from a simple and intuitive 4 steps software workflow to run all your assays.

Take Your Analysis to the Next Level, Automation!

Celigo automation

Stacker Automation: No Need to ‘Baby-Sit’ Your Plates

With the simple integration of the plate stacker, you are free to work on other tasks while Celigo screens your stack of plates.

  • Up to 50 plate capacity
  • 15 sec transfer rate between plates
  • Data exports automatically for each plate
  • Handles plates with or without lids
  • Accommodates from 6-well up to 1536-well plate formats
  • Fits on a standard lab bench
  • Ideal for endpoint assays
  • Easy to add on to existing Celigo instruments

Video: Watch the Celigo with integrated Plate Stacker 

Celigo stacker automation

Parameter Specification
Celigo only Dimensions 19 inch wide x 29 inch long x 17 inch high (with plate holder extended)
Celigo + Stacker Dimensions 25 inch wide x 44 inch long x 40 inch high (Total)
Electrical Power 5 Electrical outlets needed at 100-240VAC 50/60Hz (30Amps Total)
Compressor (sold with Stacker) 1.2 CFM @ 90 PSI with 1 gallon tank
Operating Temperature 15 ºC to 25 ºC
Operating Humidity 10% to 90% RH, non-condensing
Instrument Shipping & Storage Temperature -18 ºC to 65 ºC
Instrument Shipping & Storage Humidity 10% to 90% RH, non-condensing
Manufacturer  Nexcelom Bioscience, LLC.
360 Merrimack St., Building 9
Lawrence, MA 01843
 Kbiosystems Limited
Units 5 to 10 Paycocke Close
Basildon, Essex, UK, SS143HS
Distributor/Support Obtain information at https://www.nexcelom.com/purchase or contact support@nexcelom.com
Support Status Definition of Support Status
Recommended Recommended plates are plates have been tested, verified and used frequently on Celigo in house. They are known for good optical quality and focus. They will work in all applications.
Supported Supported plates are plates that have been tested, verified and are known to work with the Celigo instrument. They have been shown to work in most all applications.
Unsupported Unsupported plates are plate profiles that are available for the Celigo instrument that work but have been known to have imaging and/or focus issues.
Plate Name Manufacturer Well Type Support Status Compatibles
6-Well BD Falcon™ 353046 Plate Corning Clear Recommended 353224, 353934, 353846, 351146, 353502
6-Well Corning™ 3516 Plate Corning Clear Recommended 3471, 3506, 3335
6-Well CytoOne® CC7682-7506 Plate CytoOne Clear Supported
6-Well Greiner™ 657160 Plate Greiner Clear Supported 657185, 657165
6-Well Nunc™ 140675 Plate Thermo Clear Supported
12-Well Corning™ 3513 Plate Corning Clear Recommended 3336, 3512
12-Well BD Falcon™ 353043 Plate Corning Clear Supported 353224, 351143, 353503
12-Well CytoOne® CC7682-7512 Plate CytoOne Clear Supported
12-Well Nunc™ 150628 Plate Thermo Scientific Clear Unsupported
24-Well Corning™ 3524 Plate Corning Clear Recommended 3337, 3526, 3527, 3573
24-Well PE Visiplate™ 1450606 Plate Perkin Elmer Black Recommended
24-Well BD Falcon™ 353047 Plate Corning Clear Supported 353226, 353935, 353847, 351147, 358115, 354723, 356723, 354775, 356775, 353504
24-Well CytoOne® CC7682-7524 Plate CytoOne Clear Supported
24-Well Greiner™ 662160 Plate Greiner Clear Supported 662102, 622165
24-Well Seahorse™ XF24 Plate Seahorse Biosciences Clear Supported
24-Well Nunc™ 142475 Plate Thermo Scientific Clear Unsupported
48-Well Corning™ 3548 Plate Corning Clear Supported
48-Well Greiner™ 677180 Plate Greiner Clear Supported 677102, 677165
48-Well Nunc™ 150687 Plate Thermo Scientific Clear Unsupported
Nexcelom3D 96-well Ultra-low attachment treated round bottom multi-well plates Corning Clear Recommended ULA-96U
96-Well Corning™ 3603 Plate Greiner Black, White Recommended 3604, 3610, 3631, 3632, 3651, 3843, 3842, 3903, 3904, 3601, 3635, 3340, 3842, 3843
96-Well Greiner™ 655090 Plate Corning Black, White Recommended 655087, 655097, 655946, 655948, 655936, 655956, 655098, 655094, 655944
96-Well BD Falcon™ 353072 Plate Corning Clear Supported 353916, 353936, 353075, 351172, 354407, 354429, 354461, 356461, 354516, 356516, 354607, 356698, 356690, 354689, 356689, 354409, 354410, 354670, 354596, 354657
96-Well BD Falcon™ 353219 Plate Corning Black, White Supported 353377
96-Well BD Falcon™ 354640 Plate Corning Black, White Supported 354650, 356650, 354651, 356651, 356701, 356693, 354649, 356649, 356640, 356700, 356692, 356717
96-Well BD Falcon™ 356717 Plate Corning Black Supported 354717
96-Well BD Falcon™ 351177 U-Bottom Plate Corning Round bottom Supported
96-Well Corning™ 3596 Plate Corning Clear Supported 3300, 3474, 3595, 3598, 3599, 3585, 3595, 3628, 3841
96-Well Corning™ 3696 Plate Corning Half Area, Black Supported 3686, 3688, 3690, 3693, 3694, 3695, 3696, 3697
96-Well Corning™ 7007 U-Bottom Plate Corning Round bottom Supported
96-Well Greiner™ 655087 Plate Greiner Black Supported 655088
96-Well Greiner™ 655161 Plate Greiner Clear Supported 655101, 655192
96-Well Greiner™ 655180 Plate Greiner Clear, chimney Supported 655182, 655185, 655940, 655930, 655950
96-Well Greiner™ 675986 Plate Greiner Half Area, Black Supported 67509x
96-Well Greiner™ 650185 U-Bottom Plate Greiner Round bottom Supported
96-Well Nunc™ 167008 Plate Thermo Clear Supported
96-Well PE Isoplate™ 6005050 Plate Perkin Elmer Black Supported
96-Well PE Viewplate™ 6005225 Plate Perkin Elmer Black Supported
96-Well Seahorse™ FX96 Plate Seahorse Bioscience Black Supported
Nexcelom3D 384-well Ultra-low attachment treated round bottom multi-well plates Nexcelom Clear Recommended ULA-384U
384-Well BD Falcon™ 353962 Plate Corning Clear Supported
384-Well Corning™ 3542 Plate Corning Low vol, Black Recommended 3540
384-Well Corning™ 3680 Plate Corning Clear Recommended 3640, 3844, 3700, 3701, 3702, 3844
384-Well Corning™ 3712 Plate Corning Black, White Recommended 3653, 3655, 3846, 3845, 3706, 3707, 3711, 3683, 3845, 3846
384-Well Corning™ 3827 Plate Corning Low attach Supported
384-Well Greiner™ 781182 Plate Greiner Clear Supported 781185, 781186, 781061, 781940, 781930, 781950
384-Well Greiner™ 781091 Plate Greiner Black Supported 781098, 781095, 781094, 781944, 781090, 781096, 781097, 781946, 781948, 781936, 781956
1536-Well Corning™ 3838 Plate Corning Black, White Recommended 3833, 3836, 3893
1536-Well BD Falcon™ 356771 Plate Corning Black Supported
1536-Well Greiner™ 789866 Plate Greiner Black Supported 789896
1-Well Nunc™ Omnitray ThermoFisher Clear Supported 242811
TC Flasks
T25 Greiner™ 690175 Flask Greiner Clear Supported
T25 Greiner™ 690175 Flask – Single View Greiner Clear Supported
T25 BD Falcon™ 353014 Flask Greiner Clear Supported
T25 BD Falcon™ 353014 Flask – Single View Greiner Clear Supported
T75 BD Falcon™ 353136 Flask Greiner Clear Supported
T75 BD Falcon™ 353136 Flask – Single View Greiner Clear Supported
T75 Corning™ 430641 Flask Greiner Clear Unsupported
T25 Corning™ 430639 Flask Greiner Clear Unsupported
Holders for
BD Falcon 353003 Dish Corning Supported 353803
1-Slide Holder (2/3 cover slip) Nexcelom Bioscience Supported
1-Slide Holder (square cover slip) Nexcelom Bioscience Supported
4-Slide Holder (2/3 cover slip) Nexcelom Bioscience Supported
4-Slide Holder (square cover slip) Greiner Supported


  • Proprietary image acquisition and processing software
  • Powerful analysis software/Dell Precision computer
  • Windows 10


  • 1 LED-based enhanced brightfield imaging channel with uniform well illumination
  • 4 LED-based fluorescent channels
  • Proprietary F-theta lens with superior well edge-to-edge contrast
  • Galvanometric mirrors for fast imaging of large areas
  • Large chip CCD camera (2024 x 2024 pixels)
  • 1, 2, 4 or 8 μm/pixel resolution

Fluorescent Channels

Channel Excitation Dichroic Emission Typical Dyes
Blue 377/50 409 470/22 Hoechst, DAPI
Green 483/32 506 536/40 FITC, Calcein, GFP,
AlexaFluor® 488
Red 531/40 593 629/53 R-PE, PI, Texas Red, AlexaFluor® 568
Far-Red 628/40 660 688/31 DRAQ5®, AlexaFluor® 647

Plate Compatibility

  • 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
  • T-25 and T-75 flasks
  • Slides and cell arrays plate profiles available upon request

High-Speed Imaging

  • Less than 2 minutes per 384-well plate

Weight and Dimensions

  • Dimensions: 19.5″W x 16″H x 24″D (49.5cm x 40cm x 61cm)
  • Weight: 117 lbs. (53 kg)

Power Requirements

  • 110-220 VAC 50-60 Hz

Regulatory Compliance

  • CE marking

Celigo Products

Celigo S Imaging Cytometer
Celigo S Brightfield Only
Celigo S with Automation License
Celigo S Brightfield Only with Automation License
Celigo S Satellite Workstation
Celigo Imaging Cytometer – 5 Channels
Celigo Imaging Cytometer – 5 Channels with Automation License

Part Numbers


Celigo Service & Upgrades

Celigo Automation Upgrade
Celigo S Upgrade
Celigo Fluorescent Upgrade
Celigo Software Upgrade
Celigo Service Contract
Celigo Brightfield Only Service Contract

Part Numbers



Celigo 1 Slide Holder
Celigo 4 Slides Holder
Celigo 10 cm Dish Holder

Part Numbers


The Celigo Imaging Cytometer is efficient and reliable and it has helped both expedite the research process as well as give me peace of mind of its reliability!

The Celigo is a great instrument for fluorescent tumor sphere counting and even fluorescent colony counting. I would recommend it! – Deepak Kanojia, Northwestern University

The Celigo is an interesting instrument to use. I enjoyed looking at the images to see immune cell killing. Working with our representative from Nexcelom was great! He was incredibly helpful and available at all times of the day!

The Celigo is capable of accurately counting cells and greatly assists in assays that involve cell death and migration.

I worked with our local Nexcelom representative to set up a Celigo seminar presentation/demo for my lab and some others from neighboring labs. He was very accommodating to our incredibly busy schedule and even brought us lunch, with plenty of dessert! The presentation was very informative and the Nexcelom team has been very present in the lab this week to demo and answer any questions!

We had the chance to have a quick mini demo with the Celigo Imaging Cytometer, with a full-length Celigo demo scheduled for the upcoming weeks. The Celigo was great, reasonably easy to use once the settings were dialed in and allowed us to visualize the cells and their action in ways not previously possible.

The Celigo Imaging Cytometer is very convenient and makes the work less labor-intensive. This instrument is very user-friendly and accurate!

The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user friendly and the support staff is outstanding! – Pam Bogert, Mayo Clinic

We have three super heavy users for the Celigo who are becoming experts, and about four more using the Celigo on a weekly basis. So far we are all very impressed with the results! – UCLA Institute for Geno & Proteo

With the Celigo, we are performing proliferation experiments on cultured cells with ease, compared to previous methods. We can look at cyst growth and quantitate proliferation rates.

The optics on the Celigo are better than I expected, and the instrument is easy to calibrate!

I have been using the Celigo for 3D tumorsphere assays in 384 well plates and for 2D growth tracking in 6 well plates. The Celigo is relatively easy to use and the manual that comes with the software is extremely useful. The machine has allowed me to quickly analyze growth of cells in different conditions, both 2D and 3D, over time. The images taken by the instrument are crisp. Furthermore, the ability of the machine to accurately measure spheroid size and migration allows me to perform experiments in 384 well plates with ease!

The best part of working with Celigo is its ability to run analysis on the fly. Also, the assay-specific algorithms that come with the program are very useful, e.g. the wound healing analysis method with a well mask is the most convenient method I’ve used among multiple instruments and analysis software packages.

We love the Celigo as it is providing us many new ways to test and confirm our assays. It also provides us with live images of our cells post-sort and enables us to generate growth charts. – Mehrnoosh Abshari, NIH – NIDCR

The Celigo helps count many plates of adhesion cells in a quick time frame.

I liked the AOPI stain application and the rapid counting of the desired population. We are also interested in the Celigo. We just had a great Celigo seminar last week and we look forward to the in-lab demo this week! – Mahwish Natalia, Pfizer Inc.

Thanks to the Celigo, we are now performing and monitoring 3D tumor spheroid growth inhibition routinely and easily. The multiplexing capacities of the machine are used regularly for organelles visualization, apoptosis and cell cycle assays; which highly decreases our use of a standard flow cytometer and increases our throughput by using mostly 96 and 384 well plates. Overall, the Celigo is very user friendly and we are very happy with our acquisition.

One of the main cell lines for my project is pretty difficult to work with. I’m still in the early stages with the Celigo, but its confluence and apoptosis assays seem promising in helping move my project forward. It’s really nice to not have to trypsinize my cells every time I want to perform an assay with them. – Caitlin Nichols, Dana-Farber Cancer Institute

It’s relatively simple to use – it’s selfexplaining actually, reliable, and the images you get out of it are really nice, underlining your results and you can make nice presentations with the Celigo images.

The Celigo is our “go to” for low resolution high speed scans. Particularly spheroid biology. I spoke highly of the system at the Cambridge HCA talk. – Steven Titus, NIH NCATS

The automation feature on the Celigo has greatly increased our work efficiency by integrating the imager to our robotic system. – Mandy Yim, Genentech

Every night we’re scanning between 50-100 plates. You can’t look at 50-100 plates by eye, so we just come in and review the scans and data each morning. The Celigo makes everyone more efficient.

We really utilize the high-throughput aspect of the Celigo. You get really nice statistics. You get a lot of data points. Instead of only having 3 data points you get 96 data points. You can look at small changes and actually get some statistical significance out of it.

The Celigo is really multifunctional – it can do an awful lot. If you want to track growth rates, it will do it perfectly. If you want to do large analysis, like [embryoid bodies], it will be perfect.

The Celigo has allowed our department to standardize and centralize the work within the institution.