Celigo Imaging Cytometer
Microwell plate based image cytometer for adherent and suspension cells
The Celigo is a great instrument for fluorescent tumor sphere counting and even fluorescent colony counting. I would recommend it!
The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user-friendly and the support staff is outstanding!
We love the Celigo as it is providing us many new ways to test and confirm our assays. It also provides us with live images of our cells post-sort and enables us to generate growth charts.
Introduction to Celigo
Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system.
Convenient data visualization environment that facilitates data interpretation.
Automated microplate handling for either kinetic end-point analysis or time-point analysis.
Bright Field Imaging for All Well Sizes
The Celigo Image Cytometer provides the best-in-class bright field imaging for all well sizes.
API & Data Management
Application programing interface (API) and data management solution for integration into automated workflows.
Bright Field & 4 Fluorescent Channels
Bright field and four fluorescent channels (UV to Far Red) for analyzing & quantifying multiplex assays.
Customized applications for each assay – ready-to-use, does not require any image analysis expertise.
The Celigo Cell Cytometer Counts Every Cell in Every Well
Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system. The best-in-class bright field imaging capability, in combination with 4 fluorescence channels, provide high speed, fully automated imaging and quantification of suspension, adherence cells, tumor spheroids, iPSC and cancer stem cell colonies within 6, 12, 24, 48, 96, 384, 1536-well plates, T flasks, and slides.
Proprietary optics and scanning system enable fast imaging of entire well while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.
With an intuitive software interface and optional integration into automation platforms, the Celigo provides labs with increased capabilities.
The Celigo Image Cytometer Provides the Best-in-Class Brightfield Imaging for All Well Sizes
Excellent Optics for Enhanced Image Quality
Improve brightfield optical image quality at the edge of wells and eliminate edge optical distortion using an F-Theta lens for superior well edge-to-edge image contrast.
Brightfield image taken with Celigo Imaging Cytometer at the edge of a well on a 96-well microplate showing enhanced image quality and contrast.
Brightfield image taken with conventional microscope showing distortion and low contrast near the edge of the field-of-view.
Example of 12-well plate acquisition using Celigo Imaging Cytometer.
Example of 96-well plate acquisition using Celigo Imaging Cytometer.
Fast Plate Scanning for Image Acquisition and Analysis
Increase the speed of plate acquisition dramatically using novel plate scanning technology for fast acquisition. Scan a 384-well plate in brightfield in 2 min.
Ability to Scan a Variety of Plate Vessels
Scan wells of any size using automated image stitching that can view and quantify cells and colonies in vessels up to 6-well plates and 10 cm dishes.
Brightfield image of a well on a 96-well microplate showing counted cell colonies across the well.
Accurately Quantify Cells and Colonies
Accurately quantify all the cells and colonies in the well even if they do not grow uniformly across the wells.
Brightfield image of a well on a 96-well microplate showing counted adherent cells.
Accurately Measure Adherent Cells without Trypsinization
Analyze your cell sample without trypsinization to avoid losing cells and look at cells right where they grow over multiple scan times.
Brightfield and Four Fluorescent Channels (UV to Far Red) for Analyzing & Quantifying Multiplex Assays
Fluorescent images acquired with Celigo Imaging Cytometer of (a) Tubulin, Phalloidin, and DAPI, (b) Phospho-ERK and Hoechst, and (c) GFP and mCherry.
Brightfield and Multi-Channel Fluorescence Imaging
Use a combination of brightfield and fluorescent imaging allowing the development of multi-color assays ranging from UV to Far Red.
Flow-like data analysis and presentation generated by Celigo Imaging Cytometer. Fluorescence intensity (a) histogram and (b) scatter plot.
Powerful Image Analysis Software
Identify cell populations based on a variety of parameters such as morphology measurements or fluorescence intensities using a flow-like gating interface with histogram and scatter-plots graphics.
Correlation of gated scatter plot data to counted cells on acquired images.
Clear Visualization of Image and Data Correlation
Correlate the cell populations identified in gates with the cells visualized in the image using color coding overlays.
Convenient Data Visualization Environment that Facilitates Data Interpretation
Simple At-a-Glance Plate-Based Data Review
Quickly evaluate data from well to well using an at-a-glance view for each plate
384-well plate view of wells showing cell confluency on the plate.
Simple At-a-Glance Plate-Based Growth Curves
Generate time course growth curves and display them in a plate overview for at-a-glance data review.
Time-course data plot generated by Celigo Imaging Cytometer.
Easy Brightfield and Fluorescent Image Navigation
Navigate between plates, wells and time points to look at your cells growing in specific well locations.
Brightfield image of a single cell colony observed at the same location of a well on a 96-well plate.
Application Programing Interface (API) and Data Management Solution for Integration into Automated Workflows
Integrate with Robotics
Automate your assays by running the Celigo under the control of a scheduling software and integrate with robotic arms, plate stackers, automated incubators and liquid handlers.
Automated System for High Throughput Integrated Data Acquisition and Analysis
Acquire images and data analysis of hundreds of plates 24/7 automatically.
Flexible Data Analysis Method for Multiple Users
Analyze large data sets off the Celigo instrument using the Celigo Satellite Workstation and free up Celigo time to acquire more plates.
Easily Access and Manage Data using Celigo Network Database
Facilitate access to your imaging data using the Celigo network database and connect multiple instruments and satellite workstations to seamlessly acquire and analyze your data from a convenient centralized location.
Series of Customized Applications for Each Assay – Ready to Use, Does Not Require Any Image Analysis Expertise
Label-Free Brightfield Cell Analysis
Take advantage of label-free brightfield applications to avoid staining cells with toxic dyes or transfecting with fluorescent reporters.
Label-free brightfield cell counting and from (a) low count to (b) high cell count which can be used to directly generate (c) a cell growth curve
Numerous Cell Characterization Assays
Analyze images for cell counting, confluence, colonies and 3D-spheroids.
Label-free brightfield cell counting of (a) spheroids, (b) cell counting, or (c) confluence percentage.
Ability to Export Brightfield and Fluorescent Images for Publications
Capitalize on the Celigo brightfield and fluorescent image quality to take images of your cells for your records and strengthen your publications.
Time-Saving Data Processing Method
Get data on the fly by acquiring and analyzing image simultaneously.
Easy-to-Use Preset Assay Parameters for Quick Data Analysis
Perform Celigo assays using pre-defined parameters that require very few modifications from the researchers.
Simple and Intuitive Software User Interface
Benefit from a simple and intuitive 4 steps software workflow to run all your assays.
Take Your Analysis to the Next Level, Automation!
Stacker Automation: No Need to ‘Baby-Sit’ Your Plates
With the simple integration of the plate stacker, you are free to work on other tasks while Celigo screens your stack of plates.
- Up to 50 plate capacity
- 15 sec transfer rate between plates
- Data exports automatically for each plate
- Handles plates with or without lids
- Accommodates from 6-well up to 1536-well plate formats
- Fits on a standard lab bench
- Ideal for endpoint assays
- Easy to add on to existing Celigo instruments
|Celigo only Dimensions||19 inch wide x 29 inch long x 17 inch high (with plate holder extended)|
|Celigo + Stacker Dimensions||25 inch wide x 44 inch long x 40 inch high (Total)|
|Electrical Power||5 Electrical outlets needed at 100-240VAC 50/60Hz (30Amps Total)|
|Compressor (sold with Stacker)||1.2 CFM @ 90 PSI with 1 gallon tank|
|Operating Temperature||15 ºC to 25 ºC|
|Operating Humidity||10% to 90% RH, non-condensing|
|Instrument Shipping & Storage Temperature||-18 ºC to 65 ºC|
|Instrument Shipping & Storage Humidity||10% to 90% RH, non-condensing|
|Manufacturer|| Nexcelom Bioscience, LLC.
360 Merrimack St., Building 9
Lawrence, MA 01843
| Kbiosystems Limited
Units 5 to 10 Paycocke Close
Basildon, Essex, UK, SS143HS
|Distributor/Support||Obtain information at https://www.nexcelom.com/purchase or contact firstname.lastname@example.org|
|Support Status||Definition of Support Status|
|Recommended||Recommended plates are plates have been tested, verified and used frequently on Celigo in house. They are known for good optical quality and focus. They will work in all applications.|
|Supported||Supported plates are plates that have been tested, verified and are known to work with the Celigo instrument. They have been shown to work in most all applications.|
|Unsupported||Unsupported plates are plate profiles that are available for the Celigo instrument that work but have been known to have imaging and/or focus issues.|
|Plate Name||Manufacturer||Well Type||Support Status||Compatibles|
|6-Well BD Falcon™ 353046 Plate||Corning||Clear||Recommended||353224, 353934, 353846, 351146, 353502|
|6-Well Corning™ 3516 Plate||Corning||Clear||Recommended||3471, 3506, 3335|
|6-Well CytoOne® CC7682-7506 Plate||CytoOne||Clear||Supported||—|
|6-Well Greiner™ 657160 Plate||Greiner||Clear||Supported||657185, 657165|
|6-Well Nunc™ 140675 Plate||Thermo||Clear||Supported||—|
|12-Well Corning™ 3513 Plate||Corning||Clear||Recommended||3336, 3512|
|12-Well BD Falcon™ 353043 Plate||Corning||Clear||Supported||353224, 351143, 353503|
|12-Well CytoOne® CC7682-7512 Plate||CytoOne||Clear||Supported||—|
|12-Well Nunc™ 150628 Plate||Thermo Scientific||Clear||Unsupported||—|
|24-Well Corning™ 3524 Plate||Corning||Clear||Recommended||3337, 3526, 3527, 3573|
|24-Well PE Visiplate™ 1450606 Plate||Perkin Elmer||Black||Recommended||—|
|24-Well BD Falcon™ 353047 Plate||Corning||Clear||Supported||353226, 353935, 353847, 351147, 358115, 354723, 356723, 354775, 356775, 353504|
|24-Well CytoOne® CC7682-7524 Plate||CytoOne||Clear||Supported||—|
|24-Well Greiner™ 662160 Plate||Greiner||Clear||Supported||662102, 622165|
|24-Well Seahorse™ XF24 Plate||Seahorse Biosciences||Clear||Supported||—|
|24-Well Nunc™ 142475 Plate||Thermo Scientific||Clear||Unsupported||—|
|48-Well Corning™ 3548 Plate||Corning||Clear||Supported||—|
|48-Well Greiner™ 677180 Plate||Greiner||Clear||Supported||677102, 677165|
|48-Well Nunc™ 150687 Plate||Thermo Scientific||Clear||Unsupported||—|
|Nexcelom3D 96-well Ultra-low attachment treated round bottom multi-well plates||Corning||Clear||Recommended||ULA-96U|
|96-Well Corning™ 3603 Plate||Greiner||Black, White||Recommended||3604, 3610, 3631, 3632, 3651, 3843, 3842, 3903, 3904, 3601, 3635, 3340, 3842, 3843|
|96-Well Greiner™ 655090 Plate||Corning||Black, White||Recommended||655087, 655097, 655946, 655948, 655936, 655956, 655098, 655094, 655944|
|96-Well BD Falcon™ 353072 Plate||Corning||Clear||Supported||353916, 353936, 353075, 351172, 354407, 354429, 354461, 356461, 354516, 356516, 354607, 356698, 356690, 354689, 356689, 354409, 354410, 354670, 354596, 354657|
|96-Well BD Falcon™ 353219 Plate||Corning||Black, White||Supported||353377|
|96-Well BD Falcon™ 354640 Plate||Corning||Black, White||Supported||354650, 356650, 354651, 356651, 356701, 356693, 354649, 356649, 356640, 356700, 356692, 356717|
|96-Well BD Falcon™ 356717 Plate||Corning||Black||Supported||354717|
|96-Well BD Falcon™ 351177 U-Bottom Plate||Corning||Round bottom||Supported||—|
|96-Well Corning™ 3596 Plate||Corning||Clear||Supported||3300, 3474, 3595, 3598, 3599, 3585, 3595, 3628, 3841|
|96-Well Corning™ 3696 Plate||Corning||Half Area, Black||Supported||3686, 3688, 3690, 3693, 3694, 3695, 3696, 3697|
|96-Well Corning™ 7007 U-Bottom Plate||Corning||Round bottom||Supported||—|
|96-Well Greiner™ 655087 Plate||Greiner||Black||Supported||655088|
|96-Well Greiner™ 655161 Plate||Greiner||Clear||Supported||655101, 655192|
|96-Well Greiner™ 655180 Plate||Greiner||Clear, chimney||Supported||655182, 655185, 655940, 655930, 655950|
|96-Well Greiner™ 675986 Plate||Greiner||Half Area, Black||Supported||67509x|
|96-Well Greiner™ 650185 U-Bottom Plate||Greiner||Round bottom||Supported||—|
|96-Well Nunc™ 167008 Plate||Thermo||Clear||Supported||—|
|96-Well PE Isoplate™ 6005050 Plate||Perkin Elmer||Black||Supported||—|
|96-Well PE Viewplate™ 6005225 Plate||Perkin Elmer||Black||Supported||—|
|96-Well Seahorse™ FX96 Plate||Seahorse Bioscience||Black||Supported||—|
|Nexcelom3D 384-well Ultra-low attachment treated round bottom multi-well plates||Nexcelom||Clear||Recommended||ULA-384U|
|384-Well BD Falcon™ 353962 Plate||Corning||Clear||Supported||—|
|384-Well Corning™ 3542 Plate||Corning||Low vol, Black||Recommended||3540|
|384-Well Corning™ 3680 Plate||Corning||Clear||Recommended||3640, 3844, 3700, 3701, 3702, 3844|
|384-Well Corning™ 3712 Plate||Corning||Black, White||Recommended||3653, 3655, 3846, 3845, 3706, 3707, 3711, 3683, 3845, 3846|
|384-Well Corning™ 3827 Plate||Corning||Low attach||Supported||—|
|384-Well Greiner™ 781182 Plate||Greiner||Clear||Supported||781185, 781186, 781061, 781940, 781930, 781950|
|384-Well Greiner™ 781091 Plate||Greiner||Black||Supported||781098, 781095, 781094, 781944, 781090, 781096, 781097, 781946, 781948, 781936, 781956|
|1536-Well Corning™ 3838 Plate||Corning||Black, White||Recommended||3833, 3836, 3893|
|1536-Well BD Falcon™ 356771 Plate||Corning||Black||Supported||—|
|1536-Well Greiner™ 789866 Plate||Greiner||Black||Supported||789896|
|1-Well Nunc™ Omnitray||ThermoFisher||Clear||Supported||242811|
|T25 Greiner™ 690175 Flask||Greiner||Clear||Supported||—|
|T25 Greiner™ 690175 Flask – Single View||Greiner||Clear||Supported||—|
|T25 BD Falcon™ 353014 Flask||Greiner||Clear||Supported||—|
|T25 BD Falcon™ 353014 Flask – Single View||Greiner||Clear||Supported||—|
|T75 BD Falcon™ 353136 Flask||Greiner||Clear||Supported||—|
|T75 BD Falcon™ 353136 Flask – Single View||Greiner||Clear||Supported||—|
|T75 Corning™ 430641 Flask||Greiner||Clear||Unsupported||—|
|T25 Corning™ 430639 Flask||Greiner||Clear||Unsupported||—|
|BD Falcon 353003 Dish||Corning||—||Supported||353803|
|1-Slide Holder (2/3 cover slip)||Nexcelom Bioscience||—||Supported||—|
|1-Slide Holder (square cover slip)||Nexcelom Bioscience||—||Supported||—|
|4-Slide Holder (2/3 cover slip)||Nexcelom Bioscience||—||Supported||—|
|4-Slide Holder (square cover slip)||Greiner||—||Supported||—|
- Proprietary image acquisition and processing software
- Powerful analysis software/Dell Precision computer
- Windows 10
- 1 LED-based enhanced brightfield imaging channel with uniform well illumination
- 4 LED-based fluorescent channels
- Proprietary F-theta lens with superior well edge-to-edge contrast
- Galvanometric mirrors for fast imaging of large areas
- Large chip CCD camera (2024 x 2024 pixels)
- 1, 2, 4 or 8 μm/pixel resolution
|Green||483/32||506||536/40||FITC, Calcein, GFP,
|Red||531/40||593||629/53||R-PE, PI, Texas Red, AlexaFluor® 568|
|Far-Red||628/40||660||688/31||DRAQ5®, AlexaFluor® 647|
- 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
- T-25 and T-75 flasks
- Slides and cell arrays plate profiles available upon request
- Less than 2 minutes per 384-well plate
Weight and Dimensions
- Dimensions: 19.5″W x 16″H x 24″D (49.5cm x 40cm x 61cm)
- Weight: 117 lbs. (53 kg)
- 110-220 VAC 50-60 Hz
- CE marking
Celigo S Imaging Cytometer
Celigo S Brightfield Only
Celigo S with Automation License
Celigo S Brightfield Only with Automation License
Celigo S Satellite Workstation
Celigo Imaging Cytometer – 5 Channels
Celigo Imaging Cytometer – 5 Channels with Automation License
Celigo Service & Upgrades
Celigo Automation Upgrade
Celigo S Upgrade
Celigo Fluorescent Upgrade
Celigo Software Upgrade
Celigo Service Contract
Celigo Brightfield Only Service Contract
Celigo 1 Slide Holder
Celigo 4 Slides Holder
Celigo 10 cm Dish Holder
The Celigo is a great instrument for fluorescent tumor sphere counting and even fluorescent colony counting. I would recommend it! – Deepak Kanojia, Northwestern University
The Celigo is an interesting instrument to use. I enjoyed looking at the images to see immune cell killing. Working with our representative from Nexcelom was great! He was incredibly helpful and available at all times of the day!
The Celigo is capable of accurately counting cells and greatly assists in assays that involve cell death and migration.
I worked with our local Nexcelom representative to set up a Celigo seminar presentation/demo for my lab and some others from neighboring labs. He was very accommodating to our incredibly busy schedule and even brought us lunch, with plenty of dessert! The presentation was very informative and the Nexcelom team has been very present in the lab this week to demo and answer any questions!
We had the chance to have a quick mini demo with the Celigo Imaging Cytometer, with a full-length Celigo demo scheduled for the upcoming weeks. The Celigo was great, reasonably easy to use once the settings were dialed in and allowed us to visualize the cells and their action in ways not previously possible.
The Celigo Imaging Cytometer is very convenient and makes the work less labor-intensive. This instrument is very user-friendly and accurate!
The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user friendly and the support staff is outstanding! – Pam Bogert, Mayo Clinic
We have three super heavy users for the Celigo who are becoming experts, and about four more using the Celigo on a weekly basis. So far we are all very impressed with the results! – UCLA Institute for Geno & Proteo
With the Celigo, we are performing proliferation experiments on cultured cells with ease, compared to previous methods. We can look at cyst growth and quantitate proliferation rates.
The optics on the Celigo are better than I expected, and the instrument is easy to calibrate!
I have been using the Celigo for 3D tumorsphere assays in 384 well plates and for 2D growth tracking in 6 well plates. The Celigo is relatively easy to use and the manual that comes with the software is extremely useful. The machine has allowed me to quickly analyze growth of cells in different conditions, both 2D and 3D, over time. The images taken by the instrument are crisp. Furthermore, the ability of the machine to accurately measure spheroid size and migration allows me to perform experiments in 384 well plates with ease!
The best part of working with Celigo is its ability to run analysis on the fly. Also, the assay-specific algorithms that come with the program are very useful, e.g. the wound healing analysis method with a well mask is the most convenient method I’ve used among multiple instruments and analysis software packages.
We love the Celigo as it is providing us many new ways to test and confirm our assays. It also provides us with live images of our cells post-sort and enables us to generate growth charts. – Mehrnoosh Abshari, NIH – NIDCR
The Celigo helps count many plates of adhesion cells in a quick time frame.
I liked the AOPI stain application and the rapid counting of the desired population. We are also interested in the Celigo. We just had a great Celigo seminar last week and we look forward to the in-lab demo this week! – Mahwish Natalia, Pfizer Inc.
Thanks to the Celigo, we are now performing and monitoring 3D tumor spheroid growth inhibition routinely and easily. The multiplexing capacities of the machine are used regularly for organelles visualization, apoptosis and cell cycle assays; which highly decreases our use of a standard flow cytometer and increases our throughput by using mostly 96 and 384 well plates. Overall, the Celigo is very user friendly and we are very happy with our acquisition.
One of the main cell lines for my project is pretty difficult to work with. I’m still in the early stages with the Celigo, but its confluence and apoptosis assays seem promising in helping move my project forward. It’s really nice to not have to trypsinize my cells every time I want to perform an assay with them. – Caitlin Nichols, Dana-Farber Cancer Institute
It’s relatively simple to use – it’s selfexplaining actually, reliable, and the images you get out of it are really nice, underlining your results and you can make nice presentations with the Celigo images.
The Celigo is our “go to” for low resolution high speed scans. Particularly spheroid biology. I spoke highly of the system at the Cambridge HCA talk. – Steven Titus, NIH NCATS
The automation feature on the Celigo has greatly increased our work efficiency by integrating the imager to our robotic system. – Mandy Yim, Genentech
Every night we’re scanning between 50-100 plates. You can’t look at 50-100 plates by eye, so we just come in and review the scans and data each morning. The Celigo makes everyone more efficient.
We really utilize the high-throughput aspect of the Celigo. You get really nice statistics. You get a lot of data points. Instead of only having 3 data points you get 96 data points. You can look at small changes and actually get some statistical significance out of it.
The Celigo is really multifunctional – it can do an awful lot. If you want to track growth rates, it will do it perfectly. If you want to do large analysis, like [embryoid bodies], it will be perfect.
The Celigo has allowed our department to standardize and centralize the work within the institution.