Celigo Imaging Cytometer2018-10-24T16:22:29+00:00

Celigo Imaging Cytometer

Microwell plate based image cytometer for adherent and suspension cells

Celigo image cytometer with stacker automation
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The Celigo is a great instrument for fluorescent tumor sphere counting and even fluorescent colony counting. I would recommend it!

Deepak Kanojia, Northwestern University

The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user-friendly and the support staff is outstanding!

Pam Bogert, Mayo Clinic

We love the Celigo as it is providing us many new ways to test and confirm our assays. It also provides us with live images of our cells post-sort and enables us to generate growth charts.

Mehrnoosh Abshari, NIH - NIDCR
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Introduction to Celigo

Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system.

Data Visualization

Convenient data visualization environment that facilitates data interpretation.

Automation

Automated microplate handling for either kinetic end-point analysis or time-point analysis.

Bright Field Imaging for All Well Sizes

The Celigo Image Cytometer provides the best-in-class bright field imaging for all well sizes.

API & Data Management

Application programing interface (API) and data management solution for integration into automated workflows.

Bright Field & 4
Fluorescent Channels

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Bright field and four fluorescent channels (UV to Far Red) for analyzing & quantifying multiplex assays.

Customized Applications

Customized applications for each assay – ready-to-use, does not require any image analysis expertise.

Select Science Interview

Automating Cell Imaging & Counting with Celigo
Select Science interview with Jean Qiu

The Celigo Cell Cytometer Counts Every Cell in Every Well

Celigo Cell Cytometer

Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system. The best-in-class bright field imaging capability, in combination with 4 fluorescence channels, provide high speed, fully automated imaging and quantification of suspension, adherence cells, tumor spheroids, iPSC and cancer stem cell colonies within 6, 12, 24, 48, 96, 384, 1536-well plates, T flasks, and slides.

Proprietary optics and scanning system enable fast imaging of entire well while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.

With an intuitive software interface and optional integration into automation platforms, the Celigo provides labs with increased capabilities.

Celigo example images

The Celigo Image Cytometer Provides the Best-in-Class Brightfield Imaging for All Well Sizes

Excellent Optics for Enhanced Image Quality

Improve brightfield optical image quality at the edge of wells and eliminate edge optical distortion using an F-Theta lens for superior well edge-to-edge image contrast.

bright field cell imaging

Brightfield image taken with Celigo Imaging Cytometer at the edge of a well on a 96-well microplate showing enhanced image quality and contrast.

bright field cell imaging

Brightfield image taken with conventional microscope showing distortion and low contrast near the edge of the field-of-view.

Example of 12-well plate acquisition

Example of 12-well plate acquisition using Celigo Imaging Cytometer.

Example of 96-well plate acquisition

Example of 96-well plate acquisition using Celigo Imaging Cytometer.

Fast Plate Scanning for Image Acquisition and Analysis

Increase the speed of plate acquisition dramatically using novel plate scanning technology for fast acquisition. Scan a 384-well plate in brightfield in 2 min.

Ability to Scan a Variety of Plate Vessels

Scan wells of any size using automated image stitching that can view and quantify cells and colonies in vessels up to 6-well plates and 10 cm dishes.

Brightfield image of a well on a 96-well microplate

Brightfield image of a well on a 96-well microplate showing counted cell colonies across the well.

Accurately Quantify Cells and Colonies

Accurately quantify all the cells and colonies in the well even if they do not grow uniformly across the wells.

Brightfield image 96-well microplate showing counted adherent cells.

Brightfield image of a well on a 96-well microplate showing counted adherent cells.

Accurately Measure Adherent Cells without Trypsinization

Analyze your cell sample without trypsinization to avoid losing cells and look at cells right where they grow over multiple scan times.

Brightfield and Four Fluorescent Channels (UV to Far Red) for Analyzing & Quantifying Multiplex Assays

Fluorescent images acquired with Celigo Imaging Cytometer

Fluorescent images acquired with Celigo Imaging Cytometer of (a) Tubulin, Phalloidin, and DAPI, (b) Phospho-ERK and Hoechst, and (c) GFP and mCherry.

Brightfield and Multi-Channel Fluorescence Imaging

Use a combination of brightfield and fluorescent imaging allowing the development of multi-color assays ranging from UV to Far Red.

Flow-like data analysis

Flow-like data analysis and presentation generated by Celigo Imaging Cytometer. Fluorescence intensity (a) histogram and (b) scatter plot.

Powerful Image Analysis
Software

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Identify cell populations based on a variety of parameters such as morphology measurements or fluorescence intensities using a flow-like gating interface with histogram and scatter-plots graphics.

Correlation of gated scatter plot data to counted cells on acquired images

Correlation of gated scatter plot data to counted cells on acquired images.

Clear Visualization of Image and Data Correlation

Correlate the cell populations identified in gates with the cells visualized in the image using color coding overlays.

Convenient Data Visualization Environment that Facilitates Data Interpretation

Simple At-a-Glance Plate-Based Data Review

Quickly evaluate data from well to well using an at-a-glance view for each plate
384-well plate view of wells showing cell confluency

384-well plate view of wells showing cell confluency on the plate.

Simple At-a-Glance Plate-Based Growth Curves

Generate time course growth curves and display them in a plate overview for at-a-glance data review.
Time-course data plot generated by Celigo

Time-course data plot generated by Celigo Imaging Cytometer.

Easy Brightfield and Fluorescent Image Navigation

Navigate between plates, wells and time points to look at your cells growing in specific well locations.
Brightfield image of a single cell colony

Brightfield image of a single cell colony observed at the same location of a well on a 96-well plate.

Application Programing Interface (API) and Data Management Solution for Integration into Automated Workflows

Integrate with Robotics

Automate your assays by running the Celigo under the control of a scheduling software and integrate with robotic arms, plate stackers, automated incubators and liquid handlers.

Automated System for High Throughput Integrated Data Acquisition and Analysis

Acquire images and data analysis of hundreds of plates 24/7 automatically.

Flexible Data Analysis Method for Multiple Users

Analyze large data sets off the Celigo instrument using the Celigo Satellite Workstation and free up Celigo time to acquire more plates.

Easily Access and Manage Data using Celigo Network Database

Facilitate access to your imaging data using the Celigo network database and connect multiple instruments and satellite workstations to seamlessly acquire and analyze your data from a convenient centralized location.

Series of Customized Applications for Each Assay – Ready to Use, Does Not Require Any Image Analysis Expertise

Label-Free Brightfield Cell Analysis

Take advantage of label-free brightfield applications to avoid staining cells with toxic dyes or transfecting with fluorescent reporters.
brightfield cell counting, cell growth curve

Label-free brightfield cell counting and from (a) low count to (b) high cell count which can be used to directly generate (c) a cell growth curve

Numerous Cell Characterization Assays

Analyze images for cell counting, confluence, colonies and 3D-spheroids.

spheroids, cell counting, confluence percentage

Label-free brightfield cell counting of (a) spheroids, (b) cell counting, or (c) confluence percentage.

Ability to Export Brightfield and Fluorescent Images for Publications

Capitalize on the Celigo brightfield and fluorescent image quality to take images of your cells for your records and strengthen your publications.

Time-Saving Data Processing Method

Get data on the fly by acquiring and analyzing image simultaneously.

Easy-to-Use Preset Assay Parameters for Quick Data Analysis

Perform Celigo assays using pre-defined parameters that require very few modifications from the researchers.

Simple and Intuitive Software User Interface

Benefit from a simple and intuitive 4 steps software workflow to run all your assays.

Take Your Analysis to the Next Level, Automation!

Celigo automation

Stacker Automation: No Need to ‘Baby-Sit’ Your Plates

With the simple integration of the plate stacker, you are free to work on other tasks while Celigo screens your stack of plates.

  • Up to 50 plate capacity
  • 15 sec transfer rate between plates
  • Data exports automatically for each plate
  • Handles plates with or without lids
  • Accommodates from 6-well up to 1536-well plate formats
  • Fits on a standard lab bench
  • Ideal for endpoint assays
  • Easy to add on to existing Celigo instruments

Celigo stacker automation

Parameter Specification
Celigo only Dimensions 19 inch wide x 29 inch long x 17 inch high (with plate holder extended)
Celigo + Stacker Dimensions 25 inch wide x 44 inch long x 40 inch high (Total)
Electrical Power 5 Electrical outlets needed at 100-240VAC 50/60Hz (30Amps Total)
Compressor (sold with Stacker) 1.2 CFM @ 90 PSI with 1 gallon tank
Operating Temperature 15 ºC to 25 ºC
Operating Humidity 10% to 90% RH, non-condensing
Instrument Shipping & Storage Temperature -18 ºC to 65 ºC
Instrument Shipping & Storage Humidity 10% to 90% RH, non-condensing
Manufacturer  Nexcelom Bioscience, LLC.
360 Merrimack St., Building 9
Lawrence, MA 01843
 Kbiosystems Limited
Units 5 to 10 Paycocke Close
Basildon, Essex, UK, SS143HS
Distributor/Support Obtain information at https://www.nexcelom.com/purchase or contact support@nexcelom.com
Support Status Definition of Support Status
Recommended Recommended plates are plates have been tested, verified and used frequently on Celigo in house. They are known for good optical quality and focus. They will work in all applications.
Supported Supported plates are plates that have been tested, verified and are known to work with the Celigo instrument. They have been shown to work in most all applications.
Unsupported Unsupported plates are plate profiles that are available for the Celigo instrument that work but have been known to have imaging and/or focus issues.
Plate Name Manufacturer Well Type Support Status Compatibles
6-well
6-Well BD Falcon™ 353046 Plate Corning Clear Recommended 353224, 353934, 353846, 351146, 353502
6-Well Corning™ 3516 Plate Corning Clear Recommended 3471, 3506, 3335
6-Well CytoOne® CC7682-7506 Plate CytoOne Clear Supported
6-Well Greiner™ 657160 Plate Greiner Clear Supported 657185, 657165
6-Well Nunc™ 140675 Plate Thermo Clear Supported
12-well
12-Well Corning™ 3513 Plate Corning Clear Recommended 3336, 3512
12-Well BD Falcon™ 353043 Plate Corning Clear Supported 353224, 351143, 353503
12-Well CytoOne® CC7682-7512 Plate CytoOne Clear Supported
12-Well Nunc™ 150628 Plate Thermo Scientific Clear Unsupported
24-well
24-Well Corning™ 3524 Plate Corning Clear Recommended 3337, 3526, 3527, 3573
24-Well PE Visiplate™ 1450606 Plate Perkin Elmer Black Recommended
24-Well BD Falcon™ 353047 Plate Corning Clear Supported 353226, 353935, 353847, 351147, 358115, 354723, 356723, 354775, 356775, 353504
24-Well CytoOne® CC7682-7524 Plate CytoOne Clear Supported
24-Well Greiner™ 662160 Plate Greiner Clear Supported 662102, 622165
24-Well Seahorse™ XF24 Plate Seahorse Biosciences Clear Supported
24-Well Nunc™ 142475 Plate Thermo Scientific Clear Unsupported
48-well
48-Well Corning™ 3548 Plate Corning Clear Supported
48-Well Greiner™ 677180 Plate Greiner Clear Supported 677102, 677165
48-Well Nunc™ 150687 Plate Thermo Scientific Clear Unsupported
96-well
Nexcelom3D 96-well Ultra-low attachment treated round bottom multi-well plates Corning Clear Recommended ULA-96U
96-Well Corning™ 3603 Plate Greiner Black, White Recommended 3604, 3610, 3631, 3632, 3651, 3843, 3842, 3903, 3904, 3601, 3635, 3340, 3842, 3843
96-Well Greiner™ 655090 Plate Corning Black, White Recommended 655087, 655097, 655946, 655948, 655936, 655956, 655098, 655094, 655944
96-Well BD Falcon™ 353072 Plate Corning Clear Supported 353916, 353936, 353075, 351172, 354407, 354429, 354461, 356461, 354516, 356516, 354607, 356698, 356690, 354689, 356689, 354409, 354410, 354670, 354596, 354657
96-Well BD Falcon™ 353219 Plate Corning Black, White Supported 353377
96-Well BD Falcon™ 354640 Plate Corning Black, White Supported 354650, 356650, 354651, 356651, 356701, 356693, 354649, 356649, 356640, 356700, 356692, 356717
96-Well BD Falcon™ 356717 Plate Corning Black Supported 354717
96-Well BD Falcon™ 351177 U-Bottom Plate Corning Round bottom Supported
96-Well Corning™ 3596 Plate Corning Clear Supported 3300, 3474, 3595, 3598, 3599, 3585, 3595, 3628, 3841
96-Well Corning™ 3696 Plate Corning Half Area, Black Supported 3686, 3688, 3690, 3693, 3694, 3695, 3696, 3697
96-Well Corning™ 7007 U-Bottom Plate Corning Round bottom Supported
96-Well Greiner™ 655087 Plate Greiner Black Supported 655088
96-Well Greiner™ 655161 Plate Greiner Clear Supported 655101, 655192
96-Well Greiner™ 655180 Plate Greiner Clear, chimney Supported 655182, 655185, 655940, 655930, 655950
96-Well Greiner™ 675986 Plate Greiner Half Area, Black Supported 67509x
96-Well Greiner™ 650185 U-Bottom Plate Greiner Round bottom Supported
96-Well Nunc™ 167008 Plate Thermo Clear Supported
96-Well PE Isoplate™ 6005050 Plate Perkin Elmer Black Supported
96-Well PE Viewplate™ 6005225 Plate Perkin Elmer Black Supported
96-Well Seahorse™ FX96 Plate Seahorse Bioscience Black Supported
384-well
Nexcelom3D 384-well Ultra-low attachment treated round bottom multi-well plates Nexcelom Clear Recommended ULA-384U
384-Well BD Falcon™ 353962 Plate Corning Clear Supported
384-Well Corning™ 3542 Plate Corning Low vol, Black Recommended 3540
384-Well Corning™ 3680 Plate Corning Clear Recommended 3640, 3844, 3700, 3701, 3702, 3844
384-Well Corning™ 3712 Plate Corning Black, White Recommended 3653, 3655, 3846, 3845, 3706, 3707, 3711, 3683, 3845, 3846
384-Well Corning™ 3827 Plate Corning Low attach Supported
384-Well Greiner™ 781182 Plate Greiner Clear Supported 781185, 781186, 781061, 781940, 781930, 781950
384-Well Greiner™ 781091 Plate Greiner Black Supported 781098, 781095, 781094, 781944, 781090, 781096, 781097, 781946, 781948, 781936, 781956
1536-well
1536-Well Corning™ 3838 Plate Corning Black, White Recommended 3833, 3836, 3893
1536-Well BD Falcon™ 356771 Plate Corning Black Supported
1536-Well Greiner™ 789866 Plate Greiner Black Supported 789896
1-Well
1-Well Nunc™ Omnitray ThermoFisher Clear Supported 242811
TC Flasks
T25 Greiner™ 690175 Flask Greiner Clear Supported
T25 Greiner™ 690175 Flask – Single View Greiner Clear Supported
T25 BD Falcon™ 353014 Flask Greiner Clear Supported
T25 BD Falcon™ 353014 Flask – Single View Greiner Clear Supported
T75 BD Falcon™ 353136 Flask Greiner Clear Supported
T75 BD Falcon™ 353136 Flask – Single View Greiner Clear Supported
T75 Corning™ 430641 Flask Greiner Clear Unsupported
T25 Corning™ 430639 Flask Greiner Clear Unsupported
Holders for
BD Falcon 353003 Dish Corning Supported 353803
1-Slide Holder (2/3 cover slip) Nexcelom Bioscience Supported
1-Slide Holder (square cover slip) Nexcelom Bioscience Supported
4-Slide Holder (2/3 cover slip) Nexcelom Bioscience Supported
4-Slide Holder (square cover slip) Greiner Supported
Author Date Title Journal Description of Cells Description of how Celigo was used
Dominguez, Charli
November 2017
Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer
JCI insight
MDA-MB-231, BT549, and Effector NK cells.
Celigo was used to determine the cell viability after staining with propidium iodide
Kwang Ha, Tae
November 2017
Baicalein Reduces Oxidative Stress in CHO Cell Cultures and Improves Recombinant Antibody Productivity
Biotechnology Journal
rCHO cells
Celigo was used to analyze ROS level of cells stained with CM-H2DCFDA.
Xu, S
November 2017
Basic Transcription Factor 3 Is Involved in Lung Cancer Growth and Progression Supplement: Supplement
Journal of Thoracic Oncology
Human NSCLC cells NCI-H1299 and A549
Human NSCLC NCI-H1229 and A549 cells were transfected with BFTF3 shRNA lentiviral vector. Celigo was used to analyze cell proliferation, cell death, and viability of human NSCLC
H, Liu
November 2017
AK2 Is Overexpressed and Correlated with Increased Metastasis Potential and Tumorigenicity in Lung Adenocarcinoma: Supplement
Journal of Thoracic Oncology
LAD cells
Celigo cell imaging analyzer, flow cytometric analysis, cell transwell assays, Immunofluorescence test and western blotting were performed to assess the effects of AK2 on proliferation, apoptosis, cell cycle, autophagy and epithelial-to-mesenchymal (EMT) phenotypes in LAD cells in vitro.
Pan, Kung
November 2017
ERH is up-regulated in bladder cancer and regulates the proliferation and apoptosis of T24 bladder cancer cells
Journal of Thoracic Oncology
bladder cancer cell lines T24 and 5637
BrdU incorporation and Celigo assays were used to examine the cell proliferation of ERHshRNA or scrambled-shRNA cell lines
Dame, Keri
November 2017
Derivation of Thyroid Progenitors from Embryonic Stem Cells through Transient, Developmental Stage-Specific Overexpression of Nkx2-1
ProQuest Dissertations Publishing
Thyroid cells are derived from mouse embryonic stem cells
 
Filipovic, Nenad
November 2017
Selenotriapine An isostere of the most studied thiosemicarbazone with pronounced pro-apoptotic activity, low toxicity and ability to challenge phenotype reprogramming of 3-D mammary adenocarcinoma tumors
Arabian Journal of Chemistry
MCF-7 tumor models
The Celigo was used to measure the area of the spheroids. Measurments were taken over an 8 day period and spheroid size was compared to size on day 0. Growth of treated and untreated spheroids were measured.
Hanigan, Thomas
October 2017
Scaffold dependent histone deacetylase (HDAC) inhibitor induced re-equilibration of the subcellular localization and posttranslational modification state of class I HDACs
Plos One
MCF-7 cells
MCF-7 cells were fixed with methanol and stained with PI. The Celigo was used to image the cells andperform cell cycle analysis.
Quentin Li, Qingdi
October 2017
Protein kinase D inhibitor CRT0066101 suppresses bladder cancer growth in vitro and xenografts via blockade of the cell cycle at G2/M
Cellular and Molecular Life Sciences
Bladder cancer cells: T24, T24T, TCCSUP, and UMUC1. Human uroepithelial cells: SV-HUC
Bladder cancer and human uroepithelial cells were incubated with a compound called CRT0066101. After an incubation period, the cells were stained with calcein AM, PI, and Hoechst. Celigo was used to capture images of the cells and determine the viability.
Ortiz-Virumbrales, Maitane
October 2017
CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer’s PSEN2N141I neurons
Acta Neuropathologica Communications
7889(s)B, 050643 (Control), 948 (AD1), 949(fControl), and 950 (AD2) iPSC lines
Celigo was used to capture images and analyze iPSC cell lines stained with PI.
Hsu, Chih-Jung
October 2017
Trans-acting oligodeoxythymidine phosphorothioate triester reagents for transient transfection optimized and facilitated by a high-throughput microbioreactor system
Biotechnology and Applied Biochemistry
HeLa cells
The Celigo was used to analyze the “the transfection efficiency of each dTtaPS-plasmid complex”. Celigo captured images of GFP positive cells to determine the fluorescence intensity in each well.
Zhuang, Peng-Yuan
September 2017
Effect of TALEN-mediated IL-6 knockout on cell proliferation,
apoptosis, invasion and anti-cancer therapy in hepatocellular
carcinoma (HCC-LM3) cells
Oncotarget
HCCLM3-wt or HCCLM3-IL6(-) cells
HCCLM3-wt or HCCLM3-IL6(-) were treated with sorafenib or IFN_. To determine the proliferation cells, cell counting kit-8 was added to the 96-well plates. Celigo was used to determine the absorbance of cells at 490 nm.
Shaw, David
September 2017
Development and Characterization of an Automated Imaging Workflow to Generate Clonally-Derived Cell Lines for Therapeutic Proteins
Biotechnology Progress
CHO-K1 Cells
The Celigo was used to count CHO-K1 cells stained with CellTracker Green CMFDA 0 days and 21 days after plating.” Both CHO-K1 Green and CHO-K1. Red expressing CHO-K1 hosts were transfected with the same DNA preparation of mAb B” and plated into 384-well plates after electroporation. After the plates were imaged, the results were used to ” drive automated hit-picking where 1,056 wells with growth were picked at random into 96-well plates.”
Tsuboi, Setskuko
September 2017
Critical Review—Water-Soluble Near-Infrared Fluorophores Emitting over 1000 nm and Their Application to In Vivo Imaging in the Second Optical Window (1000–1400 nm)
ECS Journal of Solid State Science and Technology
HeLa cells
In order to determine the toxicity of NIR fluorophores, the Celigo was used to determine the number of HeLa cells 0-70 hours after treatment with NIR fluorophoes.
Li, Wei
September 2017
RDM1 gene Overexpression Represents a Therapeutic Target in Papillary Thyroid Carcinoma Running title: The new therapeutic target of papillary thyroid carcinoma
Endocrine Connections
K1 and TPC1 cells
K1 and TPC1 cells were stained post lentivirus transduction to determine the rate of siRNA gene knockdown. 96-well plates were scanned on the Celigo using bright field and the green fluorescent channel. After gating, the Celigo determined the total cell count and ” the average integrated red fluorescence intensity”. The Celigo was used to create a scatter plot of “green fluorescence area (in µm2) and integrated fluorescence intensity.
Zhour, Yizhou
September 2017
Beating the Odds: The Poisson Distribution of All Input Cells During Limiting Dilution Grossly Underestimates Whether a Cell Line is Clonally-Derived or Not
Biotechnology Progress
CHO-K1 cells
The Celigo was used to image plates initially and three weeks later to determine the distribution of “mAbX expressing CHO-K1 cells [that] were transfected with either mAbX-sp1 or mAbX-sp2 vectors”. 384-well plates were imaged in bright field and fluorescent channels.
Kessel, Sarah
September 2017
Real-Time Apoptosis and Viability HighThroughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer
SLAS Discovery
MCTS produced from the glioblastoma cell line U87MG
The Celigo was used to examine the “the kinetic apoptotic and cytotoxic effects” of numerous compounds of multicellular tumor spheroids. The bright field channel, blue channel, red channel, green channel, and far red channel was used on the Celigo to image and analyze the plates.
Miao, Ruoyu
September 2017
Utility of the dual-specificity protein kinase TTK as a therapeutic target for intrahepatic spread of liver cancer.
Scientific Reports
Liver Cancer Cells
The Celigo was used to perform in situ cellular analysis on liver cancer cells after TTK inhibition. They also used Annexin V-FITC/PI Hoechst Apoptosis assay on the Celigo. They performed cell cycle analysis using BrdU and DAPI stains on the Celigo. Lastly, they used the Celigo to capture bright-field images of cell growth.
Mi Gu, Sun
August 2017
A synthetic cannabinoid JWH-210 reduces lymphoid organ weights and T-cell activator levels in mice via CB2 receptors
Naunyn-Schmiedeberg’s Arch Pharmacol
Splenocytes and Thrymocytes
The Celigo was used to measure the cell viability of splenocytes after exposing cells to EthD-1 and calcein AM. It was also used to measure fluorescent intensity of splenocytes or thymocytes stained with DAPI and “secondary antibodies conjugated to AlexaFluor 488 and 594”
Briffa, Romina
August 2017
Unravelling the role of TRIB1 in colorectal cancer: a functional molecular pathology approach
EPMA Journal
three stable TRIB1 knock-down CRC cell line
The Celigo was used to monitor “cell cycle progression, proliferation, tumour growth, and invasion”.
Tomita, Kyoko
August 2017
Mixed-lineage kinase 3 pharmacological inhibition attenuates murine nonalcoholic steatohepatitis
JCI insight
BMDM or THP-1 cells
The Celgio was used to determine the quantity of migrated BMDMs and THP-1 cells that were fluorescently labeled.
He, Xuefeng
July 2017
Opa interacting protein 5 acts as an oncogene in bladder cancer
Journal of Cancer Research Clinical Oncology
BC-T24 and BC-5637
The Celigo was used to monitor cell growth of BC-T24 and BC-5637 after infection with shOIP5/shCtl. shOIP5 and shCtl fluorescence intensity was monitored using the Celigo and the cell counts were determined from the measured fluorescence intensity. This was to determine the “knockdown of shOIP5 suppressed cell growth”.
Leonidou, Andri
July 2017
Identification and Validation of Driver Kinases from Next-Generation Sequencing Data
Kinase Signaling Networks
 
 
Oliemuller, Erik
July 2017
SOX11 promotes invasive growth and DCIS progression
The Journal of Pathology
MCF10A, MCF10DCIS.com, BT474, BT549, Cal148, HCC202, MX-1, and UACC893.
Cells were grown in a ULA plate to allow spheroids to form. The Celigo was used to take images of the spheroids after four days. After fourteen days, the Celigo was used to determine the mammosphere-forming efficiency by determining the quantity of mamospheres and dividing by the number of cells plated per well. The Celigo was also used to measure the size of the spheroids and measure the fluorescence of Caspase-3.
Kessel, Sarah
June 2017
TIReal-time viability and apoptosis kinetic detection method of 3D multicellular tumor spheroids using the Celigo Image CytometerTLE
Cytometry Part A.
multicellular tumor spheroids
The Celigo was used in combination with PI and caspase 3/7 stain to measure the rate of apoptosis and the viability of multicellular tumor spheroids
Daubeuf, François
June 2017
A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the Mouse
Current Protocols in Mouse Biology
BAL cells, T cells, B cells, neutrophils, eosinophils, and macrophages
 
Cherradi, S
June 2017
Antibody targeting of claudin-1 as a potential colorectal cancer therapy
Journal of Experimental Medicine & Clinical Cancer Research
colorectal cancer cells
The Celigo was used to read 6-well plates that contained colorectal cancer cells using the single colony verification application. The tumorsphere application was also used and captured images of the tumorspheres. Also, the expression analysis assay was used to determine the fluorescent signals. During this experiment, they stained the cells with DAPI nuclear stain. Lastly, the Celigo was used to determine the apoptosis and growth rate of the tumorspheres.
de Andrade Mello, Paola
June 2017
Hyperthermia and associated changes in membrane fluidity potentiate P2X7 activation to promote tumor cell death
Oncotarget, Advance Publications
MCA38 colon cancer cells
The viability was determined after the cells were exposed to CCK-8 and the Celigo was used to capture images of the live cells.
Singh, Veena
June 2017
Performance of Biocept’s sample collection for tumor cell analysis.
Tumor Biology
BT474 (HER2 amplified) and H3112 (ALK re-arranged) cells
The Celigo was used to count BT474 (HER2 amplified) or H3112 (ALK re-arranged) cells after being thawed.
Nabbi, Arash
May 2017
ING3 promotes prostate cancer growth by activating the androgen receptor
BMC Medicine
LNCaP, PC3, and DU145 cells
The Celgio was used to monitor cell proliferation of LNCaP, PC3, and DU145 cells in a 96-well plate.
Kondo, Yasushi
May 2017
Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells
Diabetologia
hiPSC lines: 648A1 and 692D2. Fibroblast-derived hiPSC lines: 409B2, 206B6 and 201B7. hESC lines: H9, KhES1 and KhES3.
Cells were immunostained with anti-insulin antibodies and the Celigo was used to determine the induction rate of INS+ cells.
Huang, Ying
May 2017
Identification of hMex-3A and its effect on human bladder cancer cell proliferation
Oncotarget
Bladder cancer cells lines:5637 and T24
The Celigo was used to examine the proliferation after bladder cancer cells were transfected with an interference RNA sequence.
Grav, Lise
May 2017
Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
Springer Link
CHO Cells
The Celigo was used to determine the cell survival and cell confluency.
Ivanov, Delyan
May 2017
High-Throughput Spheroid Screens Using Volume, Resazurin Reduction, and Acid Phosphatase Activity
Springer Link
 
References the Celigo as being capable of measuring spheroid size and volume in high throughput.
Chan, Leo
May 2017
A high-throughput image cytometry-based screening method for the detection of IL2-induced peripheral blood mononuclear cell-mediated cytotoxicity
Cytotherapy
peripheral blood mononuclear cells (PBMC)
“Viability was determined by employing different staining techniques such as enzymatic stain, nucleic acid stain, multi-stain method, fluorescent stain, and trypan blue staining on the Celigo and Cellometer. “
Chan, Leo
May 2017
Assessment of Cell Viability with Single-, Dual-, and Multi-Staining Methods Using Image Cytometry
Springer Link
 
In this work, they “demonstrate a novel high-throughput cytotoxicity screening assay using the Celigo imaging-cytometry method”. A 96 well plate was used with the Celigo to analyze the percent lysis of cells.
Cuny, Gregory
April 2017
PYRIMIDINE COMPOUNDS AND METHODS USING THE SAME
United States Patent Application
SH-SY5Y-hTAU441 cell
The Celigo was used to assess the cytotoxicity of SH-SY5Y-hTAU441 cells.
Gheller, Brandon
April 2017
PYY regulates human skeletal muscle progenitor cell proliferation
The FASEB Journal
Skeletal Muscle stem cells (satelitte cells)
The Celigo was used to track percent confluence of cells over five days.
Blum, Jamie
April 2017
Short-term Inflammation Increases Proliferative Capacity of Human Skeletal Muscle Progenitor Cells from Young and Old Female Donors
The FASEB Journal
Muslce Progenitor Cells
The Celigo was used to track the percent confluence, number of nuclei, and percent cell death.
Smith, Paul
April 2017
Microenviroment Cytometry
Single Cell Analysis: Contemporary Research and Clinical Applications
multicellular tumor spheroids (MCTSs)
The Celigo was used to track the multicellular tumor spheroids health by staining with viability dye DRAQ7. They also looked at non-viable cells in the culture.
Zhang, Haohai
April 2017
Novel high-throughput cell-based hybridoma screening methodology using the Celigo Image Cytometer
Elsevier
Chinease hamster ovary (CHO) cells
“Demonstrate novel high-throughput screening methodology employing the Celigo Image Cytometer, which avoids nonspecific signals by contrasting antibody binding signals directly on living cells, with and without recombinant antigen expression.”
Venugopal, Jessica
April 2017
Ouabain promotes partial epithelial to mesenchymal transition (EMT) changes in human autosomal dominant polycystic kidney disease (ADPKD) cells
Elsevier
kidney disease (ADPKD) cells
The Celigo was used in combination with an inverted microscope to monitor the wound healing of a cell monolayer.
Yang, Mei-Lin
March 2017
“IL-6 ameliorates acute lung injury in influenza virus infection”
Scientific Reports
fibroblasts isolated from IL-6 -/- mice and WT mice
Fibroblasts were isolated from IL-6-/- mice and WT mice. The Celigo was used to determine the cell count, doubling time, and apoptosis rate by immunofluorescence with anti-cleaved caspase-3 antibody. It was also used to monitor the number of virus infected cells engulfed by macrophages compared to IL-6/- macrophages. The macrophages were exposed to QD649 particles and stained with DAPI.
Itoh, M
March 2017
NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture
Technology Platforms for 3D Cell Culture: A User’s Guide
 
States how NCP can be used with the Celigo for measurments.
Slawny, Nicole
March 2017
Physiologically relevant spheroid models for three‐dimensional cell culture
Technology Platforms for 3D Cell Culture: A User’s Guide
 
Lists the Celigo as a type of automated 3D assay equipment
Quentin Li, Qingdi
March 2017
Proteomic analysis of proteome and histone post-translational modifications in heat shock protein 90 inhibition-mediated bladder cancer therapeutics
Scientific Reports
5637 bladder cancer cells
They wanted to determine the antitumor impact of HSP90 inhibitors in 5637 bladder cancer cells. They stained the cells and used the Celigo to examine the cell viability.
Dall’Acqua, William
January 2017
Enhanced tumor-targeting selectivity by modulating bispecific antibody binding affinity and format valence
Scientific Reports
NCI-H358 non-small cancer cells
Cell cytotoxicity studies were performed on a Celigo S Imaging cytometer. Overlay of brightfield with either green fluorescence or blue fluorescence identified and qualified CMFDA or BMQC positive cells using Celigo Image processing software.
Li, Linfeng
January 2017
3D High-Content Screening of Organoids for Drug Discovery
Elsevier
None
This paper listed the Celigo as a 3D high content screening system
Parker, Christopher
January 2017
Ligand and Target Discovery byFragment-Based Screening in Human Cells
Elsevier
Human Cells
 
Cribbes, Scott
January 2017
A Novel Multiparametric Drug-Scoring Method for High-Throughput Screening of 3D Multicellular Tumor Spheroids Using the Celigo Image Cytometer
SAGE Journals
glioblastoma cell line U87MG
In this work, we employed the Celigo Image Cytometer to examine the effects of 14 cancer drug compounds on 3D MCTS of the glioblastoma cell line U87MG in 384-well plates.
Auberon, Florence
December 2016
Isolation of novel stilbenoids from the roots of Cyrtopodium paniculatum (Orchidaceae)
Fitoterapia
human glioblastoma U-87MG cells
Celigo
Braganza, Andrea
December 2016
UBE3B is a calmodulin-regulated, mitochondria-associated E3 ubiquitin ligase
The Journal of Biological Chemistry
human cells
The cells were counted various times after plating. Colonies were also stained with Crystal violet dye and counted with the Celigo.
Boss, Olivier
December 2016
Methods and compositions for inducing differentiation of human brown adiopocyte progenitors
FPO
adipose tissue (BAT) progenitor cells
Cells were exposed to C1-BODIPY® 500/510 C12 (Molecular Probes #D-3823) and incubated for 3 to 6 hours. C1-BODIPY® 500/510 C12 is a fluorescent fatty acid analog that is sometimes utilized for lipid trafficking studies (ThermoFisher). Next, the cells were analyzed in bright field and fluorescence. They looked at the total fluorescent intensity per well.
Wu, Yao
November 2016
Cucurbitacin-I induces hypertrophy in H9c2 cardiomyoblasts through activation of autophagy via MEK/ERK1/2 signaling pathway
Toxicology Letters
H9c2 cells
The cell viabilities were detected by the Celigo Image Cytometer
Kramer, Daniela
November 2016
Strong antitumor synergy between DNA crosslinking and HSP90 inhibition causes massive premitotic DNA fragmentation in ovarian cancer cells
Cell Death & Differentiation
human ovarian cancer cell lines
 
Su, X
November 2016
TAp63 suppresses mammary tumorigenesis through regulation of the Hippo pathway
Oncogene
mammary epithelial cells (MECs), mammary stem cells (MaSCs) and tumor-initiating cells (TICs)
 
Wu, Yao
November 2016
Cucurbitacin-I induceshypertrophy in H9c2 cardiomyoblasts through activation of autophagy viaMEK/ERK1/2 signaling pathway
Toxicology Letters
H9c2 cells
The Celigo was used to analyze cell viability of H9c2 cells
Cisneros Castillo, Liliana
October 2016
A novel computer-assisted approach to evaluate multicellular tumor spheroid invasion assay
Scientific Reports
tumor and peripheral cells
To quantify the size of MCTS in invasion assays, the present of the Celigo Cytometer is an approach that comes already with a device that takes images automatically of an inserted plate.
Kari, Vijayalakshmi
September 2016
Loss of CHD1 causes DNA repair defects and enhances prostate cancer therapeutic responsiveness
The Embo Journal
Prostate cancer cells
 
Hamilton, Duane
September 2016
Brachyury, a vaccine target, is overexpressed in triple- negative breast cancer
Society for Endocrinology
Triple-negative breast cancer cells
Nuclei were stained using DAPI and images were acquired using the Celigo S cell Imaging Cytometer
Potapova, Tamara
August 2016
Transcriptome analysis of tetraploid cells identifies cyclin D2 as a facilitator of adaptation to genome doubling in the presence of p53
Molecular Biology of the Cell
Tetraplid cells
Multi-well plates with labeled cells were imaged on Celigo Imaging Cytometer. Images were quantified and each image typically contained several hundred of cells; 3-4 images were analyzed to determine the mean number of positive cells and the standard deviation
Imamura, Yukio
August 2016
Near-Infrared Emitting Pbs Quantum Dots for in Vivo Fluorescence imaging of the Thrombotic state in septic mouse brain
Molecules
HeLa cells
HeLa cells were plated at 6000 cells/well in 96 well plates. After culturing overnight, QD1100 was added to every well at a final concentration of 0-50 nM. The number of cells in each well was counted with the Celgio after 0,7,24,4,8, and 60 hours.
Maguire, Sarah
August 2016
3D modelling identifies novel genetic dependencies associated with breast cancer progression in the isogenic MCF10 model
The Journal of Pathology
MCF10A cells
Comparison of pathway aberrations associated with progression identified that when cells are grown as 3D spheroids, they show perturbations of cancer-relevant pathways. The spheroids are was calculated using the Celigo S.
Chan, Leo Li-Yang
August 2016
A high-throughput AO/PI- based cell concentration and viability detection method using the Celigo image cytometry
Cytotechnology
Jurkat cells
The high-throughput AO/PI method described here allows for 96 well to 384-well plate samples to be analyzed in less than 7 mins, which greatly reduces the time required for the single sample based automated cell counter.
Shan, Feng
July 2016
Investigation of cancer drug penetration in 2D and 3D tumor cell culture models
University of Pittsburgh
Cal33 HNSCC cells
 
Bonasu, Surekha
July 2016
Real-time Caspase 3/7 measurement of suspension and adherent cells using the Celigo Image cytometer
Poster
Jurkat cells
Monitoring by detecting a green caspase 3/7 signal but also perform an end point assay by counterstaining the cells with Hoechst and therby determined a percent nucleated cells that are apoptosis by imaging on the Nexcelom Celigo image cytometer.
Chan, Leo Li-Yang
July 2016
A high-throughput image cytometry-based screening method for the cytotoxic effect of drug compounds on 3D tumor spheroid
Cancer Research
Cancer cells
Five experiments were conducted demonstrating comparable results in 2D and 3D models using the image based high-throughput screening method for 3D tumor spheroids on 384- well ultra low attachment round bottom microplates using the Celigo image cytometer.
Shirihai, Orian
July 2016
Lysosome acidification by photoactivated nanoparticles restores autophagy under lipotoxicity
The Journal of Cell Biology
B- cells
Analysis parameters for images acquired by the Celigo were optimized to identify individual cells based on fluorescence. Cells were then fixed in 4% paraformaldehyde, stained with DAPI, and imaged using Celigo to count the total number of cells per well for normalization.
Chan, Leo Li-Ying
June 2016
High-throughput 3D tumor spheroid screening method for cancer drug discovery using Celigo Image Cytometry
Journal of Laboratory Automation
U87MG
Celigo was used to determine the seeding density for the tumor sphere formation of the cell line U87MG, and it was to measure the IC50 value, and the dose responses of 17-AGG, paclitaxel, TMZ and doxorubicin
Ellegaard, Anne-Marie
June 2016
Repurposing Cationic amphiphilic antihistamines for cancer treatment
EbioMedicine
Non-small cell lung cancer (NSCLC)
Celigo measured the cell death after 15 minutes of propidium iodide and Hoechst-33342 staining was employeed at 37 degrees celcius.
Mazor, Yariv
June 2016
Enhancement of Immune Effector Functions by modulating IgG’s intrinsic affinity for target antigen
Immune cells, T-cells
Immune cells, T-cells
Target cells were stained with Calcein AM dye, and then seeded in a 96 well plate at a density of 1×10^4 cells/well in RPMI in 1640 with GlutaMAX with 5% FBS. Calcein AM positive cells were quantified by Celigo.
Napoli, Marco
June 2016
ΔNp63/DGCR8- Dependent MicroRNAs mediate therapeutic efficacy of HDAC inhibitors in Cancer
Cancer cell
Squamous cell carcinomas and lymphoma cells
To acess the efficacy of the compounds in affecting the protein levels of ΔNp63 and DGCR8, evaluating cells by immunofluoresence and quantified them with Celigo.
Patterson, Lisa
May 2016
In vitro assays for endothelial cell functions required for angiogenesis: Proliferation, motility, tubular differentiation, and matrix proteolysis
Angiogenesis Protocols
Umbilical vein endothelial cells, microvascular endthothelial cells
Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Kildegaard, Helene Faustrup
May 2016
Accelerated homology-directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment
Biotechnology & Bioengineering
CHO cells
Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Tomokazu, Tanaka
April 2016
Low-dose farnesyltransferase inhibitor suppresses HIF-1a and snail expression in Triple-negative breast cancer MDA-MB-231 cells in vitro
Journal of Cellular Physiology
MDA-MB-TNBC and MDA-MB-231 cells
Tumorspheres were directly counted by the Celigo Cell Cytometer at a 12-day culture. Pictures of each well were taken to conform spheres and aggregates of cells.
Mattson, Emma
April 2016
Understanding the role of RB-binding protein KDM5A in cancer cell proliferation
Illinois Mathematics and Science Academy
lung cancer cells
Focused on clarifying the role of KDM5A in a non-small cell lung cancer line under different concentrations of erlotinib, a common chemotherapy drug, by examinging the cell cycle and cell proliferation using the Celigo.
Elisabeth, Corecelle-Termeau
April 2016
Excess sphingomyelin disturbsATG9A trafficking and autophagosome closure
Autophagy
SMPD-1- deficient cells
Lysosomal areas in the indicated fibroblasts treated for 12 hours with vehicle (V) or 19.5 or 39um C12-SM were measured with Celigo adherent cell cytometer after staining.
Amy, Wagers
April 2016
Methods and compositions forrejuvenating skeletal muscle stem cells
FPO
Skeletal muscle stem cells
Wells containing myogenic colonies were either conunted by brightfield microscopy or fixed with 4% PFA and the number of cells per well was counted on a Celigo automated microscope as Hoechst-stained nuclei at the indicated time points
Gayathri, Devi
April 2016
Three-Dimensional culturesystems in cancer research: Focus on tumor spheroid model
Pharmacology & Therapeutics
Cancer stem cells
Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity, and structural complexity that reflecdt in vivo tumors.
Bidisha, Bhattacharya
April 2016
Fine-tuning of macrophageactivation using synthetic rocaglate derivates
Scientific Reports
J7-21 cells
Celigo cytometer was used ot measure number of GFP-Ipr1 positive cells and GFP-Ipr1 fluorescence intensity per nucleus after counterstaining with nuclear stain DAPI.
Peitzsch, Claudia
March 2016
An epigenetic reprogramming strategy to re-sensitize radioresistant prostate cancer cells
Cancer Research
Cancer stem cells
After 14 days, cells were anaylzed and automatically scanned using the Celigo for 30 mins at 37 degrees celcius, washed with PBS at the end of the culture, then analyzed by the Celigo.
Stanford, Elizabeth
March 2016
The role of the aryl hydrocarbonreceptor in the development of the cells with the molecular and functionalcharacteristics of cancer stem- like cells
BMC Biology
Breast cancer stem cells
After cells were treated, harvested, dosed, and plated colonies were quantified with the Celigo after eight days.
Mohammad, Tariq
January 2016
Eukaryotic translationinitiation factor 5A (elF5A) is essential for HIF-1a activiation in hypoxia
Biochemical and Biophysical research communications
Tumor spheroids
Suppression of elF5A by siRNA oligomediated knockdown or treamtent with GC7, a deoxyhypusine synthase inhibitor, led to significant reduction of HIF-1a activity
Edwin, Chang
November 2015
AshwaMAX and withaferin Ainhbits gliomas in cellular and murine orthotopic models
Journal of Neuro-oncology
GBM2, GBM39
Human parietal-cortical gliobastoma cells (GBM2, GBM39) were isolated from primary tumours while U87-MG was obtained commerically.
Linfeng, Li
November 2015
High-throughput imaging:Focusing in on drug discovery in 3D
Methods
MCT cells
3D organotypic culture models such as organoids and multicellular tumor spheroids (MCTS) are becoming more widely used for drug discovery
Henning, Gram Hensen
December 2015
Versatile microscale screeningplatform for improving recombinant protein productivity in chinese hamsterovary cells
Scientific Reports
Ovarian cells
The platform consists of four techniques compatible with 96-well microplates:lipid-based transient transfection, cell cultivation in microplates, cell counting and antibody-independent product titer determination based on split-GFP complementation
Sivapriya, Ponnurangam
December 2015
Quinomycin A targets notchsignaling pathway in pancreatic cancer stem cells
Oncotarget
CSC cells
Quinomycin treatment resulted in significant inhibition of proliferation and colony formation in pancreatic cancer cell lines, but not in normal pancreatic epithelial cells
Annika, Baude
December 2015
Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination
Nucleic Acids research
HeLa cervic carcinoma cells
POGZ-depleted cells show a similar defect in DNA damage-induced RPA2 phosphorylation as HDGFRP2-depleted cells
Andrew, Campbell
October 2015
Mutation ofataxia-telangiectasia mutated is associated with dysfunctional glutathionehomeostasis in cerebellar astroglia
Glia
Mouse cells
Cerebellar atroglia isolated from Atm mutant mice show decreased expression of the cystine/glutamate exchanger subunit xCT, glutathione (GSH) reductase, and glutathione-S-transferase.
Thilo, Riedl
October 2015
High-throughput screening forinternalizing antibodies by homogeneous fluorescence imaging of apH-activated probe
Journal of Biomolecular Screening
A431, AU565, SKOV-3 cells
The pH-activated CypHer5E label becomes fluorescent upon internalization into the acidic environment of endocytic organelles, whereas background fluorescence of noninternalized CypHer5E is minimal.
Narendran, Aru
September 2015
Targeted inhibition of MEK1 bycobimetinib leads to differentiation and apoptosis in neuroblastoma cells
BioMed central
None
 
Simeonov, Anton
September 2015
High- throughput fluorescence imaging approaches for drug discovery using in vitro and in vivo three-dimensional models
Expert Opinion Drug Discovery
None
 
Yoshida, Minoru
September 2015
Identification of thedeterminants of 2-deoxyglucose sensitivity in cancer cells by shRNA libraryscreening
ScienceDirect
Cancer cells
 
Kaji, Keisuke
September 2015
Reprogramming roadblocks are system dependent
ISSCR
(iPSC’s)
 
Anant, Shrikant
August 2015
RNA binding protein RBM3increases B-catenin signaling to increase stem cell characteristics incolorectal cancer cells
Wiley Online Library
Cancer stem cells
 
McGlennen, R
July 2015
Comparison of Antimicrobial andwound healing agents on oral fibroblast viability and In-vivo bacterial load
Omics Online
Gingival fibroblast cells
 
Dobbelstein, Matthias
July 2015
MicroRNA-101 suppresses tumorcell proliferation by acting as an endogenous proteasome inhibitor viatargeting the proteasome assembly factor POMP
ScienceDirect
Tumor cells
 
Dubrovska, Anna
June 2015
Development of novelradiochemotherapy approaches targeting prostate tumor progenitor cells usingnanohybrids
International Journal of Cancer
Cancer stem cells
 
Gahl, William
June 2015
Automated, High-throughputderivation, characterization and differentiation of induced pluripotent stemcells
Nature Methods
(iPSC’s)
 
Mermod, Nicolas
June 2015
Epigenetic regulatory elements:Recent advances in understanding their mode of action and use for recombinantprotein production in mammalian cells
Biotechnology Journal
mammalian cells
 
Lee, Dan
May 2015
High- throughput direct cell counting- based natural killer cell mediated- cytotoxicity assay using Celigo Imaging Cytometry
The Journal of Immunology
NK cells
 
Sun, Yi
May 2015
A reliable parameter tostandardize the scoring of stem cell spheres
PLOS one
Mouse embryonic stem cells
 
Jaattela, Marja
May 2015
Sensitive detection of lysosomal membrane permeabilization by lysosomal galectin puncta assay
Autophagy
 
 
Chang, Stephen
May 2015
Rapid and efficient generationof transgene-free iPSC from a small volume of cryopreserved blood
Stem cell reviews and reports
hESC
online
Kildegaard, Helene
April 2015
One-step generation of tripleknockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment
Biotechnology Journal
CHO
Online
Leach, M.O
March 2015
Acquired resistance to EGFRtyrosine kinase inhibitors alters the metabolism of human head and necksquamous carcinoma cells and xenograft tumors
British Journal of Cancer
CAL/PJ HNSCC
online
Nabergoj, Dominik, Elsa
December 2014
Evaluation of anti-inflammatory and proapoptotic activities of synthetic clathrodin analogues in human THP-1 monocytic Leukemia cells
Thesis
THP-1 cells
online
Flores, Elsa
November 2014
Non-disruptively count and quantify fluorescence iPS colonies during 2 degree reprogramming: 7 min per 6 well plate, dual- fluorescence whole well imaging cytometry
Nature
Mouse embryonic stem cells(G4)
online
Kolev, Vihren
November 2014
P13K/mTOR dual inhibitor VS-5584preferentially target cancer stem cells
American Association for Cancer Research
Cancer stem cells
To detemine tumorsphere forming efficency, cells from the tissue culture were plated and enumerated using the Celigo.
Karlin, Mondal
November 2014
The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor
Cell Reports
BT549 and MDA-MB231-LM2 breast cancer cells
Celigo was used to perform cell proliferation assays.
Westbrook, Thomas
November 2014
The oncogenic STP axis promotestriple-negative breast cancer via degradation of the REST tumor suppressor
Cell Reports
None
online
Miura, Hiromi
October 2014
Development of spheroid based high-throughput screening of cell-cell adhesion inhibitors to reverse acquired multicellular resistance
Cancer Research
A549
online
Randhawa, Zeng
October 2014
Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs
Antiviral Research
HEK293, COS7
Cell counts were evaluated by Celigo to ascertain cytotoxicity of compounds.
Glicksman, Marcie A.
October 2014
Inhibition of large T antigenATPase activity as a potential strategy to develop anti-polyomavirus JC drugs
Antiviral Research
HEK293, COS7
online
Landmann, Proia
September 2014
UDP glucuraonosyltransferase 1Aexpression levels determine the response of colorectal cancer cells to theheat shock protein 90 inhibitor ganetespib
Cell Death and Disease
human colorectal cancer cell lines
Cell confluence in drug treated and untreated wells was determined by Celigo with bright field.
Fu, Creighton
September 2014
Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase
Breast Cancer Research
MCF-7L, BT483, and T47D (human breast cancer)
Cell count was assessed on Celigo via methylene blue and bright field.
Schiff, R
September 2014
Overcoming endocrine resistancedue to reduced PTEN levels in estrogen receptor-positive breast cancer byco-targeting mammalian target of rapamycin, protein kinase B, ormitogen-activated protein kinase kinase
Breast Cancer Research
MCF7L (Human Breast Cancer), BT483, T47D
Online
Xu, Farach-Carson
July 2014
Three-dimensional in vitro tumormodels for cancer research and drug evaluation
Biotechnology Advances
tissue engineered-3D tumor models
Celigo measured spheroid diameter, permieter, and area which enabled assessments of motility, angiogenesis, and matrix invasion, as well as the actions of cell growth inhibitors.
Brix, Rafn
July 2014
Screening and identification ofsmall molecule inhibitors of ErbB2-induced invasion
Molecular Oncology
MCF-7 (human breast cancer) and SK-OV3 (human ovarian)
Cells were stained with Hoechst and PI and Celigo ascertained the total cell counts as well as number of dead cells.
Jayanthan, Ruan
July 2014
Aurora kinases as druggabletargets in pediatric leukemia: heterogeneity in target modulation activitiesand cytotoxicity by diverse novel therapeutic agents
PLOS One
pediatric and infant leukemia cell lines
Cell lines were stained with Alamar blue to assess cells survival via metabolic activity.
Scheerlinck, Van Steendam
June 2014
Detailed method description fornoninvasive monitoring of differentiation status of human embryonic stemcells
Analytical Biochemistry
human embryonic stem cells (hESCs)
Celigo evaluated colony confluence and measurement of stress level via Oct 4 expression.
Pederson, Ronda
May 2014
Accelerating genome editing inCHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool
Biotechnology and Bioengineering
CHO cells
Two-channel bright field and GFP images were captured by Celigo to count cells and determine transfection efficiency.
Lund, Kildegaard
May 2014
A versatile system for USERcloning-based assembly of expression vectors for mammalian cell engineering
PLOS One
CHO-S (Chinese hamster ovary clonal isolate)
Cells were plated in 6-well plates and transfected with GFP-tagged vectors before the addition of Hoechst stain. Two-channel (Hoechst and GFP) images were collected on the Celigo to determine cell count and transfection efficiency.
Guo, Loh
May 2014
Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening
Molecular Pharmaceutics
MCF-7 (human breast cancer)
Bright field images of the spheroids in 96-well plates were generated over a time course by Celigo. Growth kinetics of spheroid diameter, perimeter, and area were captured.
Jaiswal, Lauritzen
May 2014
S100A11 is required forefficient plasma membrane repair and survival of invasive cancer cells
Nature Communications
MCF-7 (human breast cancer)
Cell death was determined by Celigo using propidium idodide (PI) 96 hours after control or siRNA delivery.
Yang, Chung
May 2014
Systems analysis of the prostatetumor suppressor NKX3.1 supports roles in DNA repiar and luminal celldifferentiation
F1000 Research
LNCaP (human prostate cancer)
Cells were dual stained with Heochst and Alexa Fluor 594 and imaged by Celigo to ascertain cell proliferation.
Guo, Loh
May 2014
Development of a magnetic 3Dspheroid platform with potential application for high-throughput drugscreening
Molecular Pharmaceutics
multicellular tumor spheroids
Celigo captured bright field images over time to determine diameter, perimeter, and area of the spheroids with or without drug treatment.
Kalineo, Thein
April 2014
Acetaminophen and NAPQI aretoxic to auditory cells via oxidative and endoplasmic reticulumstress-dependent pathways
Hearing Research
HEI-OC1 cells (murine-derived auditory cells)
Celigo performed direct cell counts after treatment with control or drug over a time course.
Spike, Kelber
April 2014
CRIPTO/GRP78 signaling maintainsfetal and adult mammary stem cells ex vivo
Stem Cell Reports
MCF10A and fetal mammary stem cells
Celigo measured DNA content via Hoechst and cell proliferation through bright field images.
Vissapragada, Contreras
March 2014
Bidirectional crosstalk betweenperiventricular endothelial cells and neural progenitor cells promotes theformation of a neurovascular unit
Brain Research
Periventricular region endothelial cells (PVEC) and neural progenitor cells (NPC)
Cells were imaged by Celigo in 96-well Matrigel coated plates to ascertain the length and pixel intensity of 3D cords created by GFP-labeled ECs.
Holembowski, Kramer
March 2014
TAp73 is essential for germ celladhesion and maturation in testis
Journal of Cell Biology
Germ-Stertoli cell co-cultures
Celigo captured two-channel images (bright field and GFP) to quantify cells after lentiviral infection of adhesion genes.
Emhemmed, Azouaou
March 2014
Selective anticancer effects ofa synthetic flavagline on human Oct4-expressing cancer stem-like cells via ap38 MAPK-dependent caspase-3-dependent pathway
Biochemical Pharmacology
NT2/D1 (human embryonal carcinoma)
Celigo determined the rate of apoptosis via Annexin V/PI staining after flavagline derivative FL3 application.
Trapnell, Cacchiarelli
March 2014
The dynamics and regulators ofcell fate decisions are revealed by pseudotemporal ordering of single cells
Nature Biotechnology
human skeletal musucle myoblasts
Celigo performed whole well imaging with Hoechst and markers to determine the number of cells or nuclei, fraction of nueclei in MYH2- or CD13-positive cells, and total MYH2-positive area.
Shih, Chung-Hsuan
February 2014
Astroglial-Derived PeriostinPromotes Axonal Regeneration after Spinal Cord Injury
The Journal of Neuroscience
Rat Neurites
Neurite length was measured using Celigo cytometer to quantify the confluence of TujI-stained neurites, providing a quantitative measurement of neurite outgrowth.
Fu, Morrison
February 2014
Therapeutic potential of thedual EGFR/HER2 inhibitor AZD8931 in circumventing endocrine resistance
Breast Cancer Research Treatment
Tamoxifen resistant (TamRes) MCF-7 and T47D cells (both breast cancer)
Cells counts and rate of apoptosis via Annexin V-FITC staining was determined by Celigo.
Schulz, Ramona
January 2014
HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer
Cell Death and Disease
MDA-MB-453, and SK-BR-3 (Human Breast Cancer)
Cells were seeded and cultured for 1 day, then treated with CP724.714 (CP) for 24hr or left untreated and followed up to day 5. Cell confluence measured daily by Celigo. For cell survival, equal numbers of treated or untreated cells were plated into 12 well plates. With the Celigo cytometer, cell confluence was measured over indicated time periods w/Celigo
Sproul, Andrew
January 2014
Characterization and MolecularProfiling of PSEN1 Familial Alzheimers Disease iPSC Derived NeuralProgenitors
PLOS one
Fibroblast 11 and 11C, reprogramed to iPSCs
Immunostaining was performed. Hoechst was used to visualize DNA. The following antibodies were used: OCT4, SSea4, Nanog, Tra-160, Ki67, MAP2, Nestin, NeuNm TujI, NLRP2, NDP. Quantification of immunostaining was done on the Celigo 200-BFFL
Giovannini, Marco
January 2014
mTORC1 Inhibition Delays Growthof Neurofibromatosis Type 2 Schwannoma
Neuro-Oncology
Human Primary Schwann cells
Cells were fized in 4% paraformaldehyde and stained with anti-S100 protein,, followed by AI549-conjugated goat anti-rabbit secondary antibody and counterstained with Hoechst 33258. Cell surface areas were determined using the automated Celigo Cytometer.
Postupalenko, Viktoriia
January 2014
Intracellular Delivery ofFunctionally Active Proteins Using Self-AssemblingPyridulthiourea-polyehtlenimine
Journal of Controlled Release
U87 (Human Glioma), CaSki (Human small bowel mesentery), HeLa (Human Cervical Cancer)
Cells were seeded into 96-well plates at 10 4 cells/well the day before . Flow cytometry analysis was performed with a Celigo Cytometer after a 24 h incubation period on a suspension of freshly trypsinized cells
Postupalenko, Sibler
January 2014
Intracellular delivery offunctionally active proteins using self-assemblingpyridylthiourea-polyethylenimine
Journal of Controlled Release
U87 (human primary glioblastoma)
Cells were plated in 96-well plates and treated with GFP polyplexes. Celigo was used to determine intracellular eGFP delivery.
Schulz, R.
January 2014
HER2/ErbB2 Activates HSF1 andThereby Controls HSP90 Clients Including MIF in HER2-Overexpressing BreastCancer
Cell Death and Disease
 
 
Ferree, Andrew
2014
Supplemental Material-characterization and molecular profiling of PSEN1
Landes Bioscience
 
 
Venkatanarayan, Raulji
2014
IAPP-driven metabolicreprogramming induces regression of p53-deficient tumours in vivo
Nature
H1299 (human lung adenocarcinoma)
Rates of apoptosis (via Annexin V/PI staining) and ROS generation (via CellRox Deep Red) were determined by Celigo.
Chen, Hsing-Yu
March 2013
Inhibition of red/Fyn/c-CBLPathway Function by Cdc42 Controls Tumour Initiation Capacity and TamoxifenSensitivity in Basal-like Breast Cancer Cells
EMBO Molecular Medicine
MDA-MB-231, MDA-MB-468, Hs578T, HCC1569, MCF-7m HCC38, HCC70, HCC1954, MCF-10A (Human Breast Cancer)
Cells were incubated with Calcein AM and PI for 15 mins, after which live and dead cells were counted utilizing the Celigo Cytometer. Levels of intracellular stress of oxidation were determined by CMH2DCFDA staining to the manufacturers instructions. Briefly cells were incubated with CM-H2DCFDA in the phenol red free medium in teh dark for 30 min, after which cells were washed once and fluorescent intensity of cells was determined using Celigo.
Proschel, Christoph
December 2013
Delayed Transplantation ofPrecursor Cell-Derived Astrocytes Provides Multiple Benefits in a Rat Modelof Parkinsons
EMBO Molecular Medicine
Cortical and dopaminergi mid brain neurons isolated from E18.5 Sprague-Dawley rats.
Cell survival was determined by co-labelling cultures with TujI and anti-tyrosine hydroxulase antibody. TujI+ or TujI+/TH+ neurons were counted using a Celigo Cytometer.
Neradugomma, Naveen
December 2013
Prolactin Signalling EnhancesColon Cancer Stemness by Modulating Notch Singaling in a Jak2-STAT3/ERKManner
Carcinogenesis
Colon cancer cell LinesHT29, HCT116, SW480, SW620, DLD1, and normal intenstinal epithelial fetal human colon (FHC) cells
Colosphere formation was assessed after 4-6 days, the number and size of colonospheres was determined using Celigo
Xu, Cong
November 2013
A Zebrafish Embryo CultureSystem Define Factors that Promote Vertebrate Myogenesis Across Species
Cell
Zebrafish cells
Zebra fish cells were iamged after 2 days using a Celigo cytometer for GFP, mCherry and BR signals.
Neradugomma, N.
November 2013
Prolactin induces Notchsignaling in a Jak2-STAT and Jak2-ERK pathway in colon cancer stem andprogenitor cells
Carcinogenesis
 
 
Zangi, Lior
September 2013
Modified mRNA Directs the Fateof Heart Progenitor Cells and Induces Vascular Regeneration After MyocardialInfarction
Nature Biotechnology
Skeletal muscle myotubes differentiated from primary mouse satellite cells
Cells were harvested and equal numbers were plated in each well of a 96-well plate in growth media. Cells were transfected with modified RNA after 3 d in differentiation media. Myotubes were transfected w/0, 0.3, 1 or 3 µg of GFP-modified RNA. Cells were fixed 16 h after transfection. Transfected myotubes were stained for skeletal muscle myosin heavy chain, 10 µg/ml Hoechst and rabbit anti-GFP Alexa-488. Pictures were taken from the whole well using the Celigo cytometer under blue, red and green channels. Cell viability was measured by quantifying the total number of nuclei in the transfected wells 16 h after transfection and normalizing them to the total number of nuclei in the non-transfected wells.
Chen, Hsing-Yu
September 2013
MEK1/2 Inhibition SuppressesTomixfen Toxicity on CNS Glial Progenitor Cells
The Journal of Neuroscience
O-2A/OPC (Astrocyte progentior/oligodendrocyte precursor cells), GRP (Glial-restricted precursor cells), astrocytes, neuroepithelial stem cells and oligodendrocytes
Cell number and death of O-2A/OPCs were analyzed by Calcein AM and PI Staining in live culture using a Celigo Cytometer.
Ferree, Andrew
September 2013
MitoTimer Probe Reveals theImpact of Autophagy, Fusion and Motility on Subcellular Distribution of Youngand Old Mitochondrial Protein and on Relative Mitochondrial Protein Age
Autophagy
MEF (Human Breast Cancer), COS (Monkey Kidney Tissue)
To quanity red and green fluorescence intensity per cell in MEF and COS cells, cells were imaged using Celigo. Analysis parameters for images acquired by Celigo were optimized to identify individual MEF and COS cell based on fluorescence and the avg. green and red fluorescnce intensity for each cell was determined at the different time points.
Wang, Ying
August 2013
Scalable Expansion of HumanInduced Pluripotent Stem Cells in the Defined Xeno-fress E8 Medium UnderAdherent and Suspension Culture Conditions
Journal of Stem Cell Research
BC1 (human primary effusion lymphoma ), TNC1 (Rat type 1 astrocytes)
The Total # of cell aggregates in a 1ml sample was analyzed by using the embryoid body analysis module in Celigo Imaging Cytometer. The avg. equivalent diameter of aggregates and the szie of each aggregate in the sample were also measured.
He, Xianbao
August 2013
The sst1 Resistance LocusRegulates Evasion of Type 1 Interferon Signalling by Chlamydia pneumoniae asa Disease Tolerance Mechanism
PLOS one: Pathogens
Bone Marrow Derived Macrophages
T-cell survival Assay: Cells were plated and treated with infected C. pneumoniae and at designated time points were stained with Hoechst and PI. Total cell number and dead cell number were counted using Celigo Cytometer
Li, Jianqing
June 2013
A Short Hairpin RNA Screen ofInterferon-Stimulated Genes Identifies a Novel Negative Regulator of theCellular Antiviral Response
Mbio.
Vero T144 (Kidney Epithelial Cells), NIH 3T3 (Human Fibroblasts), Hek293T (Human Embryonic Kideny cells), HeLa (Human Cervical Cancer)
HeLa cells in 96-well plates were transfected with individual ISGs tagged with 3X Flag. After 24h, cells were infected w/WNV for another 24h and then fixed, permeabilized, and costained for WNV envelope protein (MAbE18)and the nucleus using DAPI. Images were captured and processed using a Celigo cytometer (Cyntellect). Infected and uninfected populations were gated separately, and infectivity was measured as the % of infected cells from the total cell counts.
Huang, Haiqing
June 2013
iPSC-Derived B-Cells ModelDiabetes Due to Glucokinase Deficiency
Journal of Clincial Investigation
Patient Cell derived IPS to B-cell Model
Quantification of positively stained cells was performed using the celigo cytometer system.
Bian, Shu
April 2013
P2X7 Integrates PI2k/AKT andAMPK-PRAS40-mTOR Signaling Pathways to Mediate Tumor Cell Death
PLOS one
MC138 (Colon Cancer Cell Line), B16/F10 (Melanoma cell line)
Cells were seeded into Corning 3603 Black 96-well plates and grown for 24 hr before exposed to ATP for a short period of time. 16–24 hr later, cell growth was evaluated using the Celigo. BR images of live cells were captured using the Celigo Cell Counting application
Escribano-Diaz, Cristina
March 2013
A Cell Cycle Dependent RegulatoryCircuit Composed of 53BP1-RIF1 and BRCA1-CtlP Controls DNA Repair PathwayChoice
Molecular Cell
U2OS (human Osteosarcoma)
U2OS cells were transfected with siRNA in 96-well plates. At 48 hr post-transfection cells were treated w/neocarzinostatin for 15 min. After a 3 hr recovery, cells were extracted with 0.2% Triton X-100 in PBS for 10 min on ice, fixed with 4% paraformaldehyde (PFA), and processed for RPA32 immofluorescence. Nuclei were counterstained with DAPI. Cells were imaged using the Celigo laser scanning plate cytometer (Brooks Automation) and analyzed with the accompanying image analysis software.
Vinci, Maria
January 2013
Tumor Spheroid Based Migration Assays for Evaluation of Therapeutic Agents
Methods in Molecular Biology
 
 
Lesovaya, Ekaterina
January 2013
Combination of a SelectiveActivator of the Glucocorticoid Receptor Compound A with a Protease Inhibitoras a Novel Strategy for Chemotherapy of Hematologic Malignancies
Cell Cycle
CEM (T- cell acute lymphoblastoma leukemia), K562 ( chronic myeloblastic leukemia), NCEB (mantle cell lymphoma) and multiple MM.1R (glucocorticoid resistant) and MM1S (glucocorticoid sensitive)
The proliferation was measured by direct cell counting using Celigo Cytometer. Cells were plated at 10^4/well onto 24-well plates and cultured in complete medium in the presence of CdpA, Dex, Bortexomib or vehicle.
Signh, Anjali
January 2013
Profiliing Pathway SpecificNovel Therapeutics in Preclinical Assessment for CNS Atypical TeratoidRhabdoid Tumors
Molecular Oncology
BT12 and BT16 (CNS ATRT), KCCF1 (cerebral spinal fluid), Hs68 (Primary skin fibroblast), T98G [EGFR over-expressing glioblastoma multiform (GBM)]
ATRT cells were trypsinized and placed in 96-well plates @ a concentration of 5×10^3 cells per well. Increasing concentrations of study agents were added to these wells to a final volu,e of 200ul per well. After 4 days, cell survival was quantified by Celigo Cytometer
Al-kasspooles, Mazin
January 2013
Preclincal Antitumor Activity ofa Nanoparticulate SN38
Journal of Investigative New Drugs
HT29, HCT116 (Human Colorectal Carcinoma) and H-meso-1 H226, H2052, MSTO-211H (Human Mesothelioma)
Cells were plated in 96-well black clear bottom plates. After overnight incubation, PI was added and live and dead cell numbers at time 0 were determined using the Celiigo cytometer. Drug treatments were then added to cells . Live and dead cell numbers were analyzed again 24, 48, and 72 after drug addition.
Singh, Lun
January 2013
Profiling pathway-specific noveltherapeutics in preclinical assessment for central nervous system atypicalteratoid rhabdoid tumors (CNS ATRT): Favorable activity of targeting EGFR-ErbB2 signaling with lapatinib
Molecular Oncology
atypical teratoid rhabdoid tumor (ATRT) cells
Cell survival in 96-well plates was determined by Celigo in bright field after 4 days treatment with drug or control.
Stecklein,
August 2012
SI Methods
PNAS
 
 
Golipour, Azadeh
December 2012
A Late Transition in SomaticCell Reprogramming Requires Regulators Distinct From the Pluripotency Network
Cell: Stem Cell
MEFs
Secondary MEFs as indicated in the schematics were stained for AO and DAPI and then scanned with a Celigo hgih-content microscope to quantigy AP-positive areas. Dox withdrawals was quantified for 4 SC and 4 SI clones using the Celigo system. In both screens, 3 days after transfection, cells were fixed and stained for Dppa24, counterstained with DAPI and then subjected to automated analysis using the Celigo system for quantifying colony formation
Smith, Karen
September 2012
Human Family with SequenceSimilarity 60 Member A (FAM60A) Protein: a New Subunit of the Sin3Deacetylase Complex
Molecular Cell Proteomic
A549, HepG2 (Human Liver Cancer)
A549 or HepG2 cells were transfected with control siRNAs or siRNAs against FAM60A. 3 days after transfection, cells were incubated for 5 hrs (A549) or 20hrs (HepG2) with BrdU. Cells were costained with DAPI. % incorporation of BrdU in each well was measured by fluorescence analysis using a Celigo Ceytometer
Stecklein, Shane
August 2012
BRCA1 amd HSP90 Cooperate inHomologous and Non-Homologous DNA Double Strand Break Repair and G2/MCheckpoint Activation
PNAS
HCC1937 (Human Breast Cancer)
DNA Synthesis, apoptosis and cell cycle distribution experiments in HCC1937 cells were performed on an LSRII flow cytometer or a Celigo adherent cell cytometer.
Nabzdyk, Christoph
August 2012
Differential Susceptibility ofHuman Primary Aortic and Coronary Artery Vascular Cells to RNA Interference
Journal of Biochemical and Biophysical Research Communications
Endothelial (EC) and vascular smooth muscle (SMC) cells
Cells were seeded at a density of 5000 cells/well of a 96-well plate. 24 hrs later cells were transfected with either non-targeting siRNA or non-targeting fluorescent red labelled siRNA using no transfection reagent., HiPerfect or Lipfectamine RNAiMax. Hoechst nuclei stain was used to label cells for counting. For data analysis an adherent cell cytometer Celigo was used.
McKevitt, I.
April 2012
SARS ORAL 2012
British Journal Of Surgery Society Ltd.
 
 
Borrego-Diaz, Emma
April 2012
Overactivation of Ras SignalingPathway in CD133+ MPNST Cells
Journal of Neurooncology
MCF7L (Human Breast Cancer)
Functional analysis of cell growth and apoptosis was performed following knockdown of miRNAs using insitu cell cytometry (Celigo)
Healy, N.A.
April 2012
The Role of miRNAs in TamoxifenResistance in Breast Cancer
Cellular and Molecular Life Sciences
Human MPNST primary cells and mouse MPNSTs, S805, S462, T530, T532, SW10 (mouse Schwann cells) and HSCs
The spheres were counted after 40hr, 7 days and 10 days of incubation using both Celigo and a light microscope.
Hsieh, Jeng-Long
March 2012
Acquisition of an EnhancedAggressive Phenotypein Human Lung Cancer Cells Slected by Suboptimal Doses ofCisplatin Following Cell Deattachment and Reattachment
Journal of Cancer Letters
A549, H1299, H1299-RI~H1299-R5 (Human Lung Cancer Cell Lines)
To analyze cell proliferation 1000 cells were seeded in 96-well plates in the complete medium at 37C on day 0. The number of cells was counted daily from day 1 to day 4 using a Celigo cytometer according to themanufacturers instructions. The proliferation rate is expressed as a ratio of the numberof cells counted on days 2-4 by the number counted on day 1
Vinci, Maria
March 2012
Advances in Establishment andAnalysis of 3D Tumor Spheroid-based Functional Assays for Target Validationand Drug Evaluation
BMC Biology
U-87 MG glioblastoma, SF188 glioblastoma, MDA-MB-231 (Human Breast Carcinoma),…37 other cancer cells lines.
For rapid, routine imaging and analysis of tumor spheroids, we utilized a Celigo cytometer, which is a bench top in situ celluar analysis system providing high quality, ful or partial images of wells using BR or FL illumination.
Huang, Han-Hung
February 2012
A Replacement for IsletEquivalents with Improved Reliability and Validity
Acta Diabetologica
Murine Pancreatic Islet cells
Isolated islet cells were iamged and counted with Celigo Cytometer
Ponnurangam, Sivapriya
February 2012
Honokiol in Combination withRadiation Targets Notch Signalling to Inhibit Colon Cancer Stem Cells
Molecular Cancer Therapeutics
HCT-116 (Human Colon Carcinoma), SW480 (Human Adenocarcinoma of the colon)
Colonosphere assay: The number and size of colonosphere were determined using Celigo
Schulz, Ramona
January 2012
Inhibiting the HSP90 ChaparoneDestabilizes Macrophage Migration Inhibitory Factor and Thereby InhibitsBreast Tumor Progression
Journal of Experimental Medicine
U2SO (Osteosarcoma), SW480 (Human adenocarcinoma of the colon)
Cell Confluence and cell numbers were evaluted over time by the Celigo Cytometer. Cell numbers (U2OS) or cell confluence (SW480) were measured using the Celigo cytometer using 49 squares per well
Gall, Jonathan
January 2012
Role of Mitofusin 2 in the RenalStress Response
PLOS one
Primary Murine Kidney Cells
Cells were fixed in 4% paraformaldehyde for 10 min, washed with PBS and stained with PBS containing Hoechst for 30 min before being imaged and counted by Celigo cytometer.
Li, Dan
January 2012
The Role of CYP3A4 mRNATranscript with Shortened 3′-Untranslated Region in HepatocyteDifferentiation, Liver Development and Repsonse to Drug Induction
Molecular Pharmacology
HepaRG Cells (Human Hepatic Cells)
The expression of RFP was analyzed in situ on an adherent cell cytometer to obtain cells images in both BR and red fuorescence, The ratios of cell densities from BR to red fluorescence in each entire well were analyzed by Celigo software.
Yoshida, Shunsuke
November 2011
Thrombospondin-2 Gene Silencingin Human Aortic Smooth Muscle Cells Improves Cell Attachment
Journal of the American College of Surgery
HAoSMC (Human Aortic Smooth Muscle Cell)
Scratch assay (wound healing attchment assay): cell positions were determined using BR and Confluence modes of Celigo, and cell counting of cells stained with Hoechst was performed
Majumdar, Ishita
November 2011
Synthetic Cyclohexenyl ChalconeNatural Products Possess Cytotoxic ActivitiesAgainst Prostate Cancer Cellsand Inhibit Cysteine Cathepsins in Vitro
Journal of Biochemical and Biophysical Research Communications
DU-145 and PC3 (Human Colon Cancer) Cells
Apoptotic cells stained with Hoechst were quantitatively analyzed with the Celigo cyotmeter
Wang, Yen-Chao
November 2011
Different Mechanisms forResistance to Trastuzumab Versus Lapatinib in HER2- Positive Breast Cancers,Role of Estrogen Receptor and HER2 Reactivation
Breast Cancer Research
BY474, UACC-812, AU-565, HCC-1569, HCC-1954, HCC-202, MDA-MB-361, MDA-MB-453, ZR75-30, SKBR-3, MCF7-HER2 (All Human Breast Cancer
Cells were incubated with Annexin V-FITC and DAPI for 30 min and analyzed by the Celigo Cytometer for Apoptosis. Cells were stained with EdU and the proliferation rate was analyzed by the Celigo cytometer
Hoeksema, Kimberly
September 2011
Systematic in-Vitro Evaluationof the NCI/NIH Developmental Therapeutics Program Approved Oncology Drug Setfor the Identification of a Candidate Drug Repertoire for MLL-RearrangedLeukemia
OncoTargets and Therapy
KOPN8 (B-Cell Precursor Leukemia), SEM (Human Acute Lymphoblastic Leukemia), B1 (Human B-cells), MOLT-3 (Human T-Lymphoblast), TIB-202 (acute monocytic leukemia) and Patient Bone Marrow Stromal (BMS) cells
Bright Field cell counting, viable cell numbers count and counting of primary leikemia samples.
Las, Guy
August 2011
Fatty Acids Suppress AutophagicTurnover in B-Cells
Journal of Biological Chemistry
INS1832/13 (Insulin-Producing ß-Cell Line)
Images of cells stained with Hoescht and PI, in paper.
Wlodkowic, Donald
May 2011
Wormomtry-on-a-Chip: InnovativeTechnologies for in Situ Analysis of Small Multicellular Organisms
Cytometry Part A.
None
Mentioned as an example of an image cytometer
Lesovaya, E.A.
May 2011
Antitumor Effect of Non-stroidGlucocorticoid Receptor Ligand CpdA on Leukemia Cell Lines CEM and K562
Biochemistry (Moscow)
K562 (human myelogenous leukemia) cells, CEM (human lymphoblastic leukemia), transfected to create CEM-NF-_B-GFP-Luc, K562-NF-_B-
GFP-Luc, CEM-AP1-GFP-Luc, and K562-AP1-GFP-Luc
Direct Count of suspension cells and a good cell count curve as an in paper figure
Nabzdyk, Christoph
April 2011
High-Throughput RNAi AssayOptimization Using Adherent Cell Cytometry
Journal of Translational Medicine
Human Aortic Smooth Muscle Cells
Cells were stained with Cell Tracker Green (Invitrogen) and with Hoechst nuclei stain. Plates were read using the adherent cell cytometer equipped with a brightfield and three fluorescent channels: a blue filter for the Hoechst, red filter for the siGLO Red, and green for the Cell Tracker Green cytoplasmic dye.
Tingen, Candace
March 2011
A Macrophage and ThecaCell-Enriched Stromal Cell Population Influences Growth and Survival ofImmature Murine Follicles in Vitro
Society for Reproduction and Fertility
Live, adhered ovarian stroma cells (Mouse)
Imaging and cell counts taken from cells stained with Hoechst and FITC
Feng, Lili
March 2011
Vascular CD39/ENTPD1 DirectlyPromotes Tumor Cell Growth by Scavenging Extracellular AdenosineTriphosphate
Neoplasia
U251 (Human Neuronal Glioblastoma), MDA-MB-231 (Human Breast Adenocarcinoma), A431 (Human Epidermal Carcinoma) and 5637 (Human Bladder Carcinoma)
Cell confluence was determined regularly for 16 days using the Celigo Cell Cytometer
Bug, M
March 2011
Anthracyclines induce theAcculumation of Mutant p53 Through E2F1-Dependant and Independent Mechanisms
Oncogene
Luciferase-expressing B16/F10, C57BL/6 (mouse melanoma) and Syngeneic C57BL/6murineMCA38 (colon cancer)
Cell growth of cells exposed to ATP was evaluated with images and cell counting using the Celigo counter. Images present in paper
Kaestner, Phillip
February 2011
Therapeutic Targeting of theMitotic Spindle Checkpoint Through Nanoparticle-Mediated siRNA DeliveryInhibits Tumor Growth in Vivo
Journal of Cancer Letters
HCT116 Cells (Human Colon Carcinoma)
Read cell numbers at 24hr increments
Wlodkowic, Donald
July 2010
Cytometry in Cell NecrobiologyRevisited. Recent Advances and New Vistas
Cytometry Part A.
None
Only mentioned as a comparable cytometric thenologu
Sirmaci, Asli
May 2010
A Truncating Mutation inSERPINB6 is Associated with Autosomal Recessive Nonsyndromic SensorineuralHearing Loss
The American Jounal of Human Genetics
HeLa cells, (Human Cervical Cancer) Transfected with SERPINB6-WT-GFP and SERPINB6-MUT-GFP
3-Channel, 8-bit stitched images were generated covering whole wells to identify the surface area & # of cells along with fluorescent intensities.
Hart, C.P.
May 2010
Product Focus: High-ContentScreening and Imaging: Instrumentation, Analysis and Applications
Journal of Biomolecular Screening
None
Product focus, describeds product capabilities

Software

  • Proprietary image acquisition and processing software
  • Powerful analysis software/Dell Precision computer
  • Windows 10

Illumination/Optics

  • 1 LED-based enhanced brightfield imaging channel with uniform well illumination
  • 4 LED-based fluorescent channels
  • Proprietary F-theta lens with superior well edge-to-edge contrast
  • Galvanometric mirrors for fast imaging of large areas
  • Large chip CCD camera (2024 x 2024 pixels)
  • 1, 2, 4 or 8 μm/pixel resolution

Fluorescent Channels

Channel Excitation Dichroic Emission Typical Dyes
Blue 377/50 409 470/22 Hoechst, DAPI
Green 483/32 506 536/40 FITC, Calcein, GFP,
AlexaFluor® 488
Red 531/40 593 629/53 R-PE, PI, Texas Red, AlexaFluor® 568
Far-Red 628/40 660 688/31 DRAQ5®, AlexaFluor® 647

Plate Compatibility

  • 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
  • T-25 and T-75 flasks
  • Slides and cell arrays plate profiles available upon request

High-Speed Imaging

  • Less than 2 minutes per 384-well plate

Weight and Dimensions

  • Dimensions: 19.5″W x 16″H x 24″D (49.5cm x 40cm x 61cm)
  • Weight: 117 lbs. (53 kg)

Power Requirements

  • 110-220 VAC 50-60 Hz

Regulatory Compliance

  • CE marking

Celigo Products

Celigo S Imaging Cytometer
Celigo S Brightfield Only
Celigo S with Automation License
Celigo S Brightfield Only with Automation License
Celigo S Satellite Workstation
Celigo Imaging Cytometer – 5 Channels
Celigo Imaging Cytometer – 5 Channels with Automation License

Part Numbers

200-BFFL-S
200-BF-S
200-BFFL-S-AUTO
200-BF-S-AUTO
200-CSW-S
200-BFFL-5C
200-BFFL-5C-AUTO

Celigo Service & Upgrades

Celigo Automation Upgrade
Celigo S Upgrade
Celigo Fluorescent Upgrade
Celigo Software Upgrade
Celigo Service Contract
Celigo Brightfield Only Service Contract

Part Numbers

200-AUTO-UPG
200-S-UPG
200-FL-UPG

Accessories

Celigo 1 Slide Holder
Celigo 4 Slides Holder
Celigo 10 cm Dish Holder

Part Numbers

200-1SL-HLDR
200-4SL-HLDR
200-10CM-HLDR

The Celigo Imaging Cytometer is efficient and reliable and it has helped both expedite the research process as well as give me peace of mind of its reliability!

The Celigo is a great instrument for fluorescent tumor sphere counting and even fluorescent colony counting. I would recommend it! – Deepak Kanojia, Northwestern University

The Celigo is an interesting instrument to use. I enjoyed looking at the images to see immune cell killing. Working with our representative from Nexcelom was great! He was incredibly helpful and available at all times of the day!

The Celigo is capable of accurately counting cells and greatly assists in assays that involve cell death and migration.

I worked with our local Nexcelom representative to set up a Celigo seminar presentation/demo for my lab and some others from neighboring labs. He was very accommodating to our incredibly busy schedule and even brought us lunch, with plenty of dessert! The presentation was very informative and the Nexcelom team has been very present in the lab this week to demo and answer any questions!

We had the chance to have a quick mini demo with the Celigo Imaging Cytometer, with a full-length Celigo demo scheduled for the upcoming weeks. The Celigo was great, reasonably easy to use once the settings were dialed in and allowed us to visualize the cells and their action in ways not previously possible.

The Celigo Imaging Cytometer is very convenient and makes the work less labor-intensive. This instrument is very user-friendly and accurate!

The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user friendly and the support staff is outstanding! – Pam Bogert, Mayo Clinic

We have three super heavy users for the Celigo who are becoming experts, and about four more using the Celigo on a weekly basis. So far we are all very impressed with the results! – UCLA Institute for Geno & Proteo

With the Celigo, we are performing proliferation experiments on cultured cells with ease, compared to previous methods. We can look at cyst growth and quantitate proliferation rates.

The optics on the Celigo are better than I expected, and the instrument is easy to calibrate!

I have been using the Celigo for 3D tumorsphere assays in 384 well plates and for 2D growth tracking in 6 well plates. The Celigo is relatively easy to use and the manual that comes with the software is extremely useful. The machine has allowed me to quickly analyze growth of cells in different conditions, both 2D and 3D, over time. The images taken by the instrument are crisp. Furthermore, the ability of the machine to accurately measure spheroid size and migration allows me to perform experiments in 384 well plates with ease!

The best part of working with Celigo is its ability to run analysis on the fly. Also, the assay-specific algorithms that come with the program are very useful, e.g. the wound healing analysis method with a well mask is the most convenient method I’ve used among multiple instruments and analysis software packages.

We love the Celigo as it is providing us many new ways to test and confirm our assays. It also provides us with live images of our cells post-sort and enables us to generate growth charts. – Mehrnoosh Abshari, NIH – NIDCR

The Celigo helps count many plates of adhesion cells in a quick time frame.

I liked the AOPI stain application and the rapid counting of the desired population. We are also interested in the Celigo. We just had a great Celigo seminar last week and we look forward to the in-lab demo this week! – Mahwish Natalia, Pfizer Inc.

Thanks to the Celigo, we are now performing and monitoring 3D tumor spheroid growth inhibition routinely and easily. The multiplexing capacities of the machine are used regularly for organelles visualization, apoptosis and cell cycle assays; which highly decreases our use of a standard flow cytometer and increases our throughput by using mostly 96 and 384 well plates. Overall, the Celigo is very user friendly and we are very happy with our acquisition.

One of the main cell lines for my project is pretty difficult to work with. I’m still in the early stages with the Celigo, but its confluence and apoptosis assays seem promising in helping move my project forward. It’s really nice to not have to trypsinize my cells every time I want to perform an assay with them. – Caitlin Nichols, Dana-Farber Cancer Institute

It’s relatively simple to use – it’s selfexplaining actually, reliable, and the images you get out of it are really nice, underlining your results and you can make nice presentations with the Celigo images.

The Celigo is our “go to” for low resolution high speed scans. Particularly spheroid biology. I spoke highly of the system at the Cambridge HCA talk. – Steven Titus, NIH NCATS

The automation feature on the Celigo has greatly increased our work efficiency by integrating the imager to our robotic system. – Mandy Yim, Genentech

Every night we’re scanning between 50-100 plates. You can’t look at 50-100 plates by eye, so we just come in and review the scans and data each morning. The Celigo makes everyone more efficient.

We really utilize the high-throughput aspect of the Celigo. You get really nice statistics. You get a lot of data points. Instead of only having 3 data points you get 96 data points. You can look at small changes and actually get some statistical significance out of it.

The Celigo is really multifunctional – it can do an awful lot. If you want to track growth rates, it will do it perfectly. If you want to do large analysis, like [embryoid bodies], it will be perfect.

The Celigo has allowed our department to standardize and centralize the work within the institution.