Celigo Imaging Cytometer
Microwell plate based image cytometer for adherent and suspension cells
See how the Celigo Imaging Cytometer works »
Introduction to Celigo
Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system designed for live cell analysis.
Convenient data visualization environment that facilitates data interpretation.
Automated microplate handling for either kinetic end-point analysis or time-point analysis.
Bright Field Imaging for All Well Sizes
The Celigo Image Cytometer provides the best-in-class bright field imaging for all well sizes.
API & Data Management
Application programing interface (API) and data management solution for integration into automated workflows.
Bright Field & 4 Fluorescent Channels
The Celigo Cell Cytometer Counts Every Cell in Every Well
Celigo is a bench-top, micro-well plate based, bright field and fluorescent imaging system designed for live cell analysis. The best-in-class bright field imaging capability, in combination with 4 fluorescence channels, provide high speed, fully automated imaging and quantification of suspension, adherence cells, tumor spheroids, iPSC and cancer stem cell colonies within 6, 12, 24, 48, 96, 384, 1536-well plates, T flasks, and slides.
Proprietary optics and scanning system enable fast imaging of entire well while maintaining consistent illumination and contrast out to the well edge, for accurate identification of all cells within each well.
With an intuitive software interface and optional integration into automation platforms, the Celigo provides labs with increased capabilities.
The Celigo Image Cytometer Provides the Best-in-Class Brightfield Imaging for All Well Sizes
Excellent Optics for Enhanced Image Quality
Improve brightfield optical image quality at the edge of wells and eliminate edge optical distortion using an F-Theta lens for superior well edge-to-edge image contrast.
Brightfield image taken with Celigo Imaging Cytometer at the edge of a well on a 96-well microplate showing enhanced image quality and contrast.
Brightfield image taken with conventional microscope showing distortion and low contrast near the edge of the field-of-view.
Example of 12-well plate acquisition using Celigo Imaging Cytometer.
Example of 96-well plate acquisition using Celigo Imaging Cytometer.
Fast Plate Scanning for Image Acquisition and Analysis
Increase the speed of plate acquisition dramatically using novel plate scanning technology for fast acquisition. Scan a 384-well plate in brightfield in 2 min.
Ability to Scan a Variety of Plate Vessels
Scan wells of any size using automated image stitching that can view and quantify cells and colonies in vessels up to 6-well plates and 10 cm dishes.
Accurately Quantify Cells and Colonies
Accurately quantify all the cells and colonies in the well even if they do not grow uniformly across the wells.
Brightfield image of a well on a 96-well microplate showing counted cell colonies across the well.
Accurately Measure Adherent Cells without Trypsinization
Analyze your cell sample without trypsinization to avoid losing cells and look at cells right where they grow over multiple scan times.
Brightfield image of a well on a 96-well microplate showing counted adherent cells.
Brightfield and Four Fluorescent Channels (UV to Far Red) for Analyzing & Quantifying Multiplex Assays
Fluorescent images acquired with Celigo Imaging Cytometer of (a) Tubulin, Phalloidin, and DAPI, (b) Phospho-ERK and Hoechst, and (c) GFP and mCherry.
Brightfield and Multi-Channel Fluorescence Imaging
Use a combination of brightfield and fluorescent imaging allowing the development of multi-color assays ranging from UV to Far Red.
Flow-like data analysis and presentation generated by Celigo Imaging Cytometer. Fluorescence intensity (a) histogram and (b) scatter plot.
Powerful Image Analysis Software
Correlation of gated scatter plot data to counted cells on acquired images.
Clear Visualization of Image and Data Correlation
Correlate the cell populations identified in gates with the cells visualized in the image using color coding overlays.
Convenient Data Visualization Environment that Facilitates Data Interpretation
Simple At-a-Glance Plate-Based Data Review
Quickly evaluate data from well to well using an at-a-glance view for each plate.
384-well plate view of wells showing cell confluency on the plate.
Simple At-a-Glance Plate-Based Growth Curves
Generate time course growth curves and display them in a plate overview for at-a-glance data review.
Time-course data plot generated by Celigo Imaging Cytometer.
Easy Brightfield and Fluorescent Image Navigation
Navigate between plates, wells and time points to look at your cells growing in specific well locations.
Brightfield image of a single cell colony observed at the same location of a well on a 96-well plate.
Application Programing Interface (API) and Data Management Solution for Integration into Automated Workflows
Integrate with Robotics
Automate your assays by running the Celigo under the control of a scheduling software and integrate with robotic arms, plate stackers, automated incubators and liquid handlers.
Automated System for High Throughput Integrated Data Acquisition and Analysis
Acquire images and data analysis of hundreds of plates 24/7 automatically.
Time-Saving Data Processing Method
Get data on the fly by acquiring and analyzing image simultaneously.
Flexible Data Analysis Method for Multiple Users
Analyze large data sets off the Celigo instrument using the Celigo Satellite Workstation and free up Celigo time to acquire more plates.
Series of Customized Applications for Each Assay – Ready to Use, Does Not Require Any Image Analysis Expertise
Label-Free Brightfield Cell Analysis
Take advantage of label-free brightfield applications to avoid staining cells with toxic dyes or transfecting with fluorescent reporters.
Label-free brightfield cell counting and from (a) low count to (b) high cell count which can be used to directly generate (c) a cell growth curve
Numerous Cell Characterization Assays
Live cell analysis of images for cell counting, confluence, colonies and 3D-spheroids.
Label-free brightfield cell counting of (a) spheroids, (b) cell counting, or (c) confluence percentage.
Ability to Export Brightfield and Fluorescent Images for Publications
Capitalize on the Celigo brightfield and fluorescent image quality to take images of your cells for your records and strengthen your publications.
Easily Access and Manage Data using Celigo Network Database
Facilitate access to your imaging data using the Celigo network database and connect multiple instruments and satellite workstations to seamlessly acquire and analyze your data from a convenient centralized location.
Easy-to-Use Preset Assay Parameters for Quick Data Analysis
Perform Celigo assays using pre-defined parameters that require very few modifications from the researchers.
Simple and Intuitive Software User Interface
Benefit from a simple and intuitive 4 steps software workflow to run all your assays.
Take Your Analysis to the Next Level, Automation!
Stacker Automation: No Need to ‘Baby-Sit’ Your Plates
With the simple integration of the plate stacker, you are free to work on other tasks while Celigo screens your stack of plates.
- Up to 50 plate capacity
- 15 sec transfer rate between plates
- Data exports automatically for each plate
- Handles plates with or without lids
- Accommodates from 6-well up to 1536-well plate formats
- Fits on a standard lab bench
- Ideal for endpoint assays
- Easy to add on to existing Celigo instruments
|Celigo only Dimensions||19 inch wide x 29 inch long x 17 inch high (with plate holder extended)|
|Celigo + Stacker Dimensions||25 inch wide x 44 inch long x 40 inch high (Total)|
|Electrical Power||5 Electrical outlets needed at 100-240VAC 50/60Hz (30Amps Total)|
|Compressor (sold with Stacker)||1.2 CFM @ 90 PSI with 1 gallon tank|
|Operating Temperature||15 ºC to 25 ºC|
|Operating Humidity||10% to 90% RH, non-condensing|
|Instrument Shipping & Storage Temperature||-18 ºC to 65 ºC|
|Instrument Shipping & Storage Humidity||10% to 90% RH, non-condensing|
|Manufacturer||Nexcelom Bioscience, LLC.
360 Merrimack St., Building 9
Lawrence, MA 01843Kbiosystems Limited
Units 5 to 10 Paycocke Close
Basildon, Essex, UK, SS143HS
|Distributor/Support||Obtain information at https://www.nexcelom.com/purchase or contact email@example.com|
|Plate Name||Manufacturer||Well Type||Compatibles|
|6-Well BD Falcon™ 353046 Plate||Corning||Clear||353224, 353934, 353846, 351146, 353502|
|6-Well Corning™ 3516 Plate||Corning||Clear||3471, 3506, 3335|
|6-Well CytoOne® CC7682-7506 Plate||CytoOne||Clear||—|
|6-Well Greiner™ 657160 Plate||Greiner||Clear||657185, 657165|
|6-Well Nunc™ 140675 Plate||Thermo||Clear||—|
|12-Well BD Falcon™ 353043 Plate||Corning||Clear||353224, 351143, 353503|
|12-Well Corning™ 3513 Plate||Corning||Clear||3336, 3512|
|12-Well CytoOne® CC7682-7512 Plate||CytoOne||Clear||—|
|24-Well BD Falcon 353047 Plate||Corning||Clear||353226, 353935, 353847, 351147, 358115,354723, 356723, 354775,
|24-Well Corning 3524 Plate||Corning||Clear||3337, 3526, 3527, 3573|
|24-Well CytoOne CC7682-7524 Plate||CytoOne||Clear||—|
|24-Well Greiner 662160 Plate||Greiner||Clear||662102, 622165|
|24-Well PE Visiplate 1450606 Plate||Perkin Elmer||Black||—|
|24-Well Seahorse XF24 Plate||Seahorse Biosciences||Clear||—|
|48-Well Corning 3548 Plate||Corning||Clear||—|
|48-Well Greiner 677180 Plate||Greiner||Clear||677102, 677165|
|96-Well BD Falcon 353219 Plate||Corning||Black, White||353377|
|96-Well BD Falcon 354640 Plate||Corning||Black, White||354650, 356650, 354651, 356651, 356701, 356693, 354649, 356649, 356640, 356700, 356692, 356717|
|96-Well BD Falcon 356717 Plate||Corning||Black||354717|
|96-Well BD Falcon 351177 U-Bottom Plate||Corning||Clear, Round Bottom ULA||—|
|96-Well BD Falcon 353072 Plate||Corning||Clear||353916, 353936, 353075, 351172, 354407, 354429, 354461, 356461,
354516, 356516, 354607, 356698, 356690, 354689, 356689, 354409, 354410,
354670, 354596, 354657
|96-Well BD Falcon 353219 Plate||Corning||Black, White||353377|
|96-Well BD Falcon 354640 Plate||Corning||Black, White||354650, 356650, 354651, 356651, 356701, 356693, 354649, 356649,
356640, 356700, 356692, 356717
|96-Well BD Falcon 356717 Plate||Corning||Black||354717|
|96-Well BD Falcon 351177 U-Bottom Plate||Corning||Clear, Round Bottom||—|
|96-Well Corning 3596 Plate||Corning||Clear||3300, 3474, 3595, 3598, 3599, 3585, 3595, 3628, 3841|
|96-Well Corning 3596 Plate||Corning||Clear||3300, 3474, 3595, 3598, 3599, 3585, 3595, 3628, 3841|
|96-Well Corning 3603 Plate||Corning||Black, White||3604, 3610, 3631, 3632, 3651, 3843, 3842, 3903, 3904, 3601,
3635, 3340, 3842, 3843
|96-Well Corning 3696 Plate||Corning||Half Area, Black||3686, 3688, 3690, 3693, 3694, 3695, 3696, 3697|
|96-Well Corning 7007 U-Bottom Plate||Corning||Clear, Round Bottom||3366, 3797, 3360, 3367, 3788, 3795, 3798, 3605, 3789, 3792,
|96-Well Greiner 655090 Plate||Greiner||Black, White||655087, 655097, 655946, 655948, 655936, 655956, 655098,
|96-Well Greiner 655087 Plate||Greiner||Black||655088|
|96-Well Greiner 655161 Plate||Greiner||Clear||655101, 655192|
|96-Well Greiner 655180 Plate||Greiner||Clear, chimney||655182, 655185, 655940, 655930, 655950|
|96-Well Greiner 675986 Plate||Greiner||Half Area, Black||67509x|
|96-Well Greiner 650185 U-Bottom Plate||Greiner||Clear, Round Bottom ULA||—|
|96-well Nexcelom ULA-96U Plate||Nexcelom
|Clear, Round Bottom, ULA||—|
|96-Well Nunc 167008 Plate||Thermo||Clear||—|
|96-Well PE Viewplate 6005225 Plate||Perkin Elmer||Black||—|
|96-Well PE Isoplate 6005050 Plate||Perkin Elmer||Black||—|
|96-Well Seahorse FX96 Plate||Seahorse Bioscience||Black||—|
|384-well Nexcelom ULA-384U Plate||Nexcelom
|Clear, Round Bottom, ULA||—|
|384-Well BD Falcon 353962 Plate||Corning||Clear||—|
|384-Well Corning 3542 Plate||Corning||Low volume, Black||3540|
|384-Well Corning 3680 Plate||Corning||Clear||3640, 3844, 3700, 3701, 3702, 3844|
|384-Well Corning 3712 Plate||Corning||Black, White||3653, 3655, 3846, 3845, 3706, 3707, 3711, 3683, 3845, 3846|
|384-Well Corning 3827 Plate||Corning||Low attach||—|
|384-Well Greiner 781182 Plate||Greiner||Clear||781185, 781186, 781061, 781940, 781930, 781950|
|384-Well Greiner 781091 Plate||Greiner||Black||781098, 781095, 781094, 781944, 781090, 781096, 781097, 781946, 781948, 781936, 781956|
|1536-Well Corning 3838 Plate||Corning||Black, White||3833, 3836, 3893|
|1536-Well BD Falcon 356771 Plate||Corning||Black||—|
|1536-Well Greiner 789866 Plate||Greiner||Black||789896|
|1-Well Nunc Omnitray||Thermo Fisher||Clear||242811|
|T25 Greiner 690175 Flask||Greiner||Clear||—|
|T25 Greiner 690175 Flask – Single View||Greiner||Clear||—|
|T25 BD Falcon 353014 Flask||Corning||Clear||—|
|T25 BD Falcon 353014 Flask – Single View||Corning||Clear||—|
|T75 BD Falcon 353136 Flask||Corning||Clear||—|
|T75 BD Falcon 353136 Flask – Single View||Corning||Clear||—|
|10cm Dish BD Falcon 353003 Dish||Corning||Clear||353803|
|1-Slide Holder (2/3 cover slip)||Nexcelom
|1-Slide Holder (square cover slip)||Nexcelom
|4-Slide Holder (2/3 cover slip)||Nexcelom
|4-Slide Holder (square cover slip)||Nexcelom
- Proprietary image acquisition and processing software
- Powerful analysis software/Dell Precision computer
- Windows 10
- 1 LED-based enhanced brightfield imaging channel with uniform well illumination
- 4 LED-based fluorescent channels
- Proprietary F-theta lens with superior well edge-to-edge contrast
- Galvanometric mirrors for fast imaging of large areas
- Large chip CCD camera (2024 x 2024 pixels)
- 1, 2, 4 or 8 μm/pixel resolution
|Green||483/32||506||536/40||FITC, Calcein, GFP,
|Red||531/40||593||629/53||R-PE, PI, Texas Red, AlexaFluor® 568|
|Far-Red||628/40||660||688/31||DRAQ5®, AlexaFluor® 647|
- 6, 12, 24 48, 96, 384, 1536 well plates (black, white and clear wall plates)
- T-25 and T-75 flasks
- Slides and cell arrays plate profiles available upon request
- Less than 2 minutes per 384-well plate
Weight and Dimensions
- Dimensions: 19.5″W x 16″H x 24″D (49.5cm x 40cm x 61cm)
- Weight: 117 lbs. (53 kg)
- 110-220 VAC 50-60 Hz
- CE marking
Celigo S Imaging Cytometer
Celigo S Brightfield Only
Celigo S with Automation License
Celigo S Brightfield Only with Automation License
Celigo S Satellite Workstation
Celigo Imaging Cytometer – 5 Channels
Celigo Imaging Cytometer – 5 Channels with Automation License
Celigo Service & Upgrades
Celigo Automation Upgrade
Celigo S Upgrade
Celigo Fluorescent Upgrade
Celigo Software Upgrade
Celigo Service Contract
Celigo Brightfield Only Service Contract
Celigo 1 Slide Holder
Celigo 4 Slides Holder
Celigo 10 cm Dish Holder
|Application||Product Name||Description||Product Number|
|Apoptosis||ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture||Measure apoptosis in 2D and 3D cultures to perform both real-time kinetic and end point assays.||CS1-V0002-1|
|Apoptosis||ViaStain™ Live Caspase 3/7 Detection for 2D/3D Culture with Hoechst||Measure apoptosis in 2D and 3D cultures for end point assays with Hoechst staining.||CSK-V0003-1|
|Apoptosis||ViaStain™ No-Wash Annexin V-FITC Kit for Celigo||A no-wash assay designed to discriminate between healthy, apoptotic, dead cells by simultaneously staining the cells with Annexin V, propidium iodide (PI) and Hoechst||CSK-V0007-1|
|Proliferation/Tracer||ViaStain™ CFSE||A green cell tracer dye used for labeling live cells and monitoring proliferation over multiple generations.||CS1-P0002-1|
|Proliferation/Tracer||ViaStain™ CMFDA||A green cell tracer dye used for labeling live cells and is retained in the cells over several generations.||CS1-P0001-1|
|Tracer||ViaStain™ Tracer Blue||A dye used for labeling live cells. Once it enters the cells, the blue color dye is retained inside live cells.||CS1-P0003-1|
|Tracer||ViaStain™ Dead Cell Nuclear Blue||Bright blue dye designed to detect dead nucleated cells in an end point assay. No wash step, add and read assay.||CSK-V0015-1|
|Tracer||ViaStain™ Dead Cell Nuclear Far Red||Bright far red dye designed to detect dead nucleated cells in an end point assay. No wash step, add and read assay.||CSK-V0014-1|
|Tracer||ViaStain™ Dead Cell Nuclear Green||Bright green dye designed to detect dead nucleated cells in an end point assay. No wash step, add and read assay.||CSK-V0012-1|
|Tracer||ViaStain™ Dead Cell Nuclear Red||Bright red dye designed to detect dead nucleated cells in an end point assay. No wash step, add and read assay.||CSK-V0013-1|
|Tracer||ViaStain™ Total Cell Nuclear Far Red||Bright blue dye designed to detect nucleated cells in an end point assay. No wash step, add and read assay.||CSK-V0010-1|
|Tracer||ViaStain™ Total Cell Nuclear Green||Bright green dye designed to detect nucleated cells in an end point assay. No wash step, add and read assay.||CSK-V0008-1|
|Tracer||ViaStain™ Total Cell Nuclear Red||Bright red dye designed to detect nucleated cells in an end point assay. No wash step, add and read assay.||CSK-V0009-1|
|Viability||DAPI||A fluorescent dye that binds to DNA. Excited by UV light, and may be used to stain fixed cells.||CS1-0127-2mL|
|Viability||Hoechst 33342||A fluorescent dye, excited by UV light, that binds to DNA. More cell membrane permeable than other Hoechst dyes, and can be used to stain live cells.||CS1-0128-5mL|
|Viability||ViaStain™ Calcein AM/Hoechst/PI Viability kit||Live and metabolically active cells are labled with Calcein, dead cells are labeled with PI, and total number of cells is determined by Hoechst.||CSK-V0006-1|
|Viability||ViaStain™ Hoechst/PI||A no wash assay kit designed to determine cell viability by staining dead cells with PI and all cell with Hoechst 33342||CSK-V0005-1|
|Viability||ViaStain™ PI Staining Solution||Propidium Iodide: a membrane impermeable staining solution for the staining of dead nucleated cells.||CS1-0109-5mL|
|Viability||ViaStain™ AO Staining Solution||Acridine Orange: a membrane permeable staining solution for the detection of nucleated cells.||CS1-0108-5mL|
|Viability||ViaStain™ AOPI Staining Solution||A staining solution for the detection of live and dead nucleated mammalian cells.||CS2-0106-5mL
- A rapid high-throughput 3D tumor spheroid image cytometry screening method for drug discovery
- Imaging and Analysis of 3D Patient-Derived Organoids Using the Celigo Automated Image Cytometer
- Real-time kinetic viability and apoptosis detection of 3D multicellular tumor spheroids using the Image Cytometer
- Automated high-throughput method for assessing pathogenic infectious dose (TCID50) using Celigo imaging cytometer
- Novel cell-based high-throughput hybridoma screening method using the Celigo image cytometer for antibody discovery
- Validating and optimizing single cell sorting of FACS using Celigo image cytometry
- High-throughput detection of CRISPR/Cas 9 gene editing efficiency, cell proliferation/viability, and monoclonality validation using Celigo Image Cytometer
- Automation Method to Increase Efficiency in Cell Line Development
- Automated label free growth tracking and culture management within flasks and multi well plates
- High-throughput counting of crystal violet stained plaques
- Viral titration assay using adherent cells
- Kinetic apoptosis using Caspase 3/7
- Celigo provides an alternative method to Cell Titer-Glo for proliferation studies in suspension cells
- Normalization of Agilent SeahorseTM XF Data with Bright Field-Based Direct Cell Counting
- Cell Line Development – Single Cell Detection, Clonal Validation, Transfection
Below are some of the most recent publications referencing Celigo. For a comprehensive listing, please see all the publications here.
|Author||Title||Journal||Date||Description of cells||How Celigo was used|
|Li, Qin||Silencing of synaptotagmin 13 inhibits tumor growth through suppressing proliferation and promoting apoptosis of colorectal cancer cells||International Journal of Molecular Medicine||January 2020||RKO and HCT116||Celigo cell counting assay was performed to detect the effect of SYT13 knockdown on the proliferation of RKO and HCT116 cells. Cell images were captured using Celigo image cytometer once per day for 5 days. The cell numbers were also quantified by using Celigo image cytometer.|
|Tong, Xing||TINAG mutation as a genetic cause of pectus excavatum||Medical hypotheses||January 2020||hFOB1.19 cells||Cell proliferation was inhibited in TINAG-silenced hFOB1.19 cells, as demonstrated by the results of Celigo imaging cytometry (P<0.01, TINAG-shRNA group vs. control group), suggesting that TINAG plays an important role in cell survival. Thus, PE may be caused by the abnormal expression of TINAG.|
|Kudsy, Mary||NUC-7738, a novel ProTide modification of 3’-deoxyadenosine, activates AMPK and kills renal cancer cells in vitro||Molecular Cancer Therapeutics||December 2019||Nine renal cancer cell lines||The effect of NUC-7738 on the growth and confluence of nine renal cancer cell lines was assessed using SRB assay and Celigo scanner, under both 0.5% oxygen and normoxic conditions.|
|Zheng, Yongchang||GTSE1, CDC20, PCNA, and MCM6 Synergistically Affect Regulations in Cell Cycle and Indicate Poor Prognosis in Liver Cancer||Analytical Cellular Pathology: The Journal of the European Society for Analytical Cellular Pathology||December 2019||Human hepatoma cell BEL-7404||Cells transfected with shRNA lentivirus stably expressed green fluorescence protein (GFP), and the adherent cell cytometry system Celigo allowed rapid imaging and quantification of cellular fluorescence expression After plating, plates were analyzed using Celigo equipped with green fluorescence channel every 24 h for 5 days.|
|Heinze, Annekathrin||The Synergistic Use of IL-15 and IL-21 for the Generation of NK Cells From CD3/CD19-Depleted Grafts Improves Their ex vivo Expansion and Cytotoxic Potential Against Neuroblastoma: Perspective for Optimized Immunotherapy Post Haploidentical Stem Cell Transplantation||Frontiers in Immunology||December 2019||Tumor spheroids produced from SK-N-AS cells and co-incubated with CIK or NK cells||Tumor spheroids were produced from 10,000 SK-N-AS cells and co-incubated with 200,000 CIK or NK cells. As a control the dynamics of tumor spheroids without effector cells were observed. The cultures were imaged via a Celigo cell cytometer after 6 h, 24 h, 3, 5, 8 and up to 10 days.|
|Masci, Allyson||Integration of Fluorescence Detection and Image-Based Automated Counting Increases Speed, Sensitivity, and Robustness of Plaque Assays||Molecular Therapy Methods & Clinical Development||September 2019||V27 cells, VERO cells||Results demonstrate that automated plaque counting by the Celigo imager is comparable to the traditional method of counting and highlights the potential to eliminate subjectivity in analyst to analyst bias. The implementation of the Celigo technology highlights the improvements in assay throughput and robustness by simple solutions to traditional plaque assays.|
|Schneider, Deborah||The E3 ubiquitin ligase RNF40 suppresses apoptosis in colorectal cancer cells||Clinical Epigenetics Journal||July 2019||HCT116, HT-29, RKO, SW48, and SW837 cells||Proliferation was tested by seeding 2000–5000 cells onto 96-well assay plates and measuring the confluence daily using a Celigo S cell imaging cytometer.|
|Wu, Lun||Gene expression alterations of human liver cancer cells following borax exposure||Oncology Reports||May 2019||HepG2 Cells Infected with Adenovirus||The plates were scanned using Celigo Image Cytometer Instrumentation to acquire images every 24 h, measuring the number of viable cells with 5-day sequential monitoring.|
|Lucotti, Serena||Aspirin blocks formation of metastatic intravascular niches by inhibiting platelet-derived COX-1/thromboxane A2||The Journal of Clinical Investigation||March 2019||CTCs||For transwell migration and invasion assays, 5000 CTCs were resuspended in serum-free DMEM and seeded on a Transwell insert coated with growth factor reduced Matrigel matrix. After 20 hours, cells adherent to the bottom well were fixed with 2% PFA, and GFP+ cells were counted with a Celigo|
|Yuan, Yuan||MAT2B promotes proliferation and inhibits apoptosis in osteosarcoma by targeting epidermal growth factor receptor and proliferating cell nuclear antigen||International Journal of Oncology||March 2019||U-2OS and MNNG/HOS cells||U-2OS cells with green fluorescence were observed and counted using a Nexcelom Celigo Image Cytometer.|
More reviews of Celigo
The Celigo Imaging Cytometer is efficient and reliable and it has helped both expedite the research process as well as give me peace of mind of its reliability!
The Celigo is a great instrument for fluorescent tumor sphere counting and even fluorescent colony counting. I would recommend it! – Deepak Kanojia, Northwestern University
The Celigo is an interesting instrument to use. I enjoyed looking at the images to see immune cell killing. Working with our representative from Nexcelom was great! He was incredibly helpful and available at all times of the day!
The Celigo is capable of accurately counting cells and greatly assists in assays that involve cell death and migration.
I worked with our local Nexcelom representative to set up a Celigo seminar presentation/demo for my lab and some others from neighboring labs. He was very accommodating to our incredibly busy schedule and even brought us lunch, with plenty of dessert! The presentation was very informative and the Nexcelom team has been very present in the lab this week to demo and answer any questions!
We had the chance to have a quick mini demo with the Celigo Imaging Cytometer, with a full-length Celigo demo scheduled for the upcoming weeks. The Celigo was great, reasonably easy to use once the settings were dialed in and allowed us to visualize the cells and their action in ways not previously possible.
The Celigo Imaging Cytometer is very convenient and makes the work less labor-intensive. This instrument is very user-friendly and accurate!
The aspects of being able to image proliferation are key to our experiments. The Celigo Imaging Cytometer is very user friendly and the support staff is outstanding! – Pam Bogert, Mayo Clinic
We have three super heavy users for the Celigo who are becoming experts, and about four more using the Celigo on a weekly basis. So far we are all very impressed with the results! – UCLA Institute for Geno & Proteo
With the Celigo, we are performing proliferation experiments on cultured cells with ease, compared to previous methods. We can look at cyst growth and quantitate proliferation rates.
The optics on the Celigo are better than I expected, and the instrument is easy to calibrate!
I have been using the Celigo for 3D tumorsphere assays in 384 well plates and for 2D growth tracking in 6 well plates. The Celigo is relatively easy to use and the manual that comes with the software is extremely useful. The machine has allowed me to quickly analyze growth of cells in different conditions, both 2D and 3D, over time. The images taken by the instrument are crisp. Furthermore, the ability of the machine to accurately measure spheroid size and migration allows me to perform experiments in 384 well plates with ease!
The best part of working with Celigo is its ability to run analysis on the fly. Also, the assay-specific algorithms that come with the program are very useful, e.g. the wound healing analysis method with a well mask is the most convenient method I’ve used among multiple instruments and analysis software packages.
We love the Celigo as it is providing us many new ways to test and confirm our assays. It also provides us with live images of our cells post-sort and enables us to generate growth charts. – Mehrnoosh Abshari, NIH – NIDCR
The Celigo helps count many plates of adhesion cells in a quick time frame.
I liked the AOPI stain application and the rapid counting of the desired population. We are also interested in the Celigo. We just had a great Celigo seminar last week and we look forward to the in-lab demo this week! – Mahwish Natalia, Pfizer Inc.
Thanks to the Celigo, we are now performing and monitoring 3D tumor spheroid growth inhibition routinely and easily. The multiplexing capacities of the machine are used regularly for organelles visualization, apoptosis and cell cycle assays; which highly decreases our use of a standard flow cytometer and increases our throughput by using mostly 96 and 384 well plates. Overall, the Celigo is very user friendly and we are very happy with our acquisition.
One of the main cell lines for my project is pretty difficult to work with. I’m still in the early stages with the Celigo, but its confluence and apoptosis assays seem promising in helping move my project forward. It’s really nice to not have to trypsinize my cells every time I want to perform an assay with them. – Caitlin Nichols, Dana-Farber Cancer Institute
It’s relatively simple to use – it’s selfexplaining actually, reliable, and the images you get out of it are really nice, underlining your results and you can make nice presentations with the Celigo images.
The Celigo is our “go to” for low resolution high speed scans. Particularly spheroid biology. I spoke highly of the system at the Cambridge HCA talk. – Steven Titus, NIH NCATS
The automation feature on the Celigo has greatly increased our work efficiency by integrating the imager to our robotic system. – Mandy Yim, Genentech
Every night we’re scanning between 50-100 plates. You can’t look at 50-100 plates by eye, so we just come in and review the scans and data each morning. The Celigo makes everyone more efficient.
We really utilize the high-throughput aspect of the Celigo. You get really nice statistics. You get a lot of data points. Instead of only having 3 data points you get 96 data points. You can look at small changes and actually get some statistical significance out of it.
The Celigo is really multifunctional – it can do an awful lot. If you want to track growth rates, it will do it perfectly. If you want to do large analysis, like [embryoid bodies], it will be perfect.
The Celigo has allowed our department to standardize and centralize the work within the institution.