///Cellometer Vision CBA Image Cytometer
Cellometer Vision CBA Image Cytometer 2018-06-26T16:55:02+00:00

Cellometer Vision CBA Image Cytometer

Dual fluorescent image cytometer for 20 µL cell-based assays

Why Vision CBA?

  1. Simple, 20 µl Cell-Based Assays
  2. Nucleated Cell Counting & Viability without Lysing
  3. User-Changeable Fluorescence Optics Modules
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The Cellometer Vision CBA is user-friendly, making it easy to understand the software and it delivers fast results!

Lyanne Suarez, University of Puerto Rico Aguadilla

The Cellometer Vision CBA Image Cytometer is a great machine! It gives very precise results in a very short time, and only uses a small amount of sample, which makes it very efficient for research purposes.

Nelson Franqui, Darlington Building

The ease at which I can count bone marrow samples without Red Cell Lysis buffer to get total nucleated cell counts quickly and accurately with the Cellometer Vision CBA has allowed our research to expand drastically!

Daniel Kuebler, Franciscan University

Our customers include:

Introduction to the Cellometer Vision CBA Image Cytometer

Simple, 20µl Cell-Based Assays

The Vision CBA System combines the simplicity of image cytometry with the power of flow analysis software to offer user-friendly cell-based assays, featuring:

  • Validated assay kits and reagents
  • Simple staining and analysis procedures
  • Default settings for automated data generation
  • No washing. No clogging. No daily calibration.
Cellometer Vision CBA - Image Cytometer Count vs Intensity Graph

Cellometer Vision CBA - Colored Sub-Population Plot of Intensity

Growing Menu of Fluorescent Cell-Based Assays

  • Apoptosis (Annexin V-FITC / PI)
  • Apoptosis (Caspase Activity)
  • Autophagy (CytoID-green)
  • Cell Proliferation (CFSE)
  • Cell Cycle (PI)
  • GFP, RFP, YFP Transfection
  • Mitochondrial Potential / Early Cell Death (JC-1)
  • Multi-Drug Resistance (ABC Transporter)
  • Surface Marker Analysis (PE, APC, FITC)
  • Viability (AO/PI, Trypan blue)
  • Vitality (Calcein-AM / PI)

Flow-Like Data Output

Vision CBA and FCS Express Software enable automated data output to pre-set data layouts for individual cell-based assays. Users have the option to modify the automatic gating with instant visual display and data update.

  • Cell Images
  • Data Tables
  • Dot Plots
  • Histograms

Data layouts can be modified to accommodate multi-sample analysis and create customized analysis and / or quality control reports.

Cellometer Vision CBA - Data Plot of Caspase Protein Activity

Nucleated Cell Counting and Viability in <60 Seconds

Count Live Nucleated Cells

Fluorescent dyes that stain DNA can be used to identify nucleated cells in a mixed cell population. Acridine orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence.

Live cells stained with AO

Cellometer Vision CBA - Live Cells Stained with AO

Count Dead Nucleated Cells

Fluorescent membrane-exclusion dyes that stain DNA can be used to identify dead nucleated cells in a mixed cell population. Propidiom iodide (PI) is a nuclear staining dye that can only enter cells with compromised membranes. Healthy cells exclude the dye. Dead nucleated cells stained with both AO and PI fluoresce red due to fluorescence resonance energy transfer.

Dead cells stained with PI

Cellometer Vision CBA - Dead Cells Stained with PI

No Interference from RBCs, Platelets, or Debris

Because platelets and mature mammalian red blood cells don’t contain nuclei, only mononuclear cells produce a fluorescent signal. Fluorescent-positive live and dead nucleated cells are easily counted and red blood cells are excluded from cell counts. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step. This also allows technicians to use a single viability method for analysis of both fresh and processed blood, bone marrow, and digested tissue samples.

Cellometer Vision CBA - Execution of Red Blood Cells from Cell Counts

Red blood cells seen in the bright field image (red arrows, left image) are not visible in the fluorescent image on the right. Red blood cells are excluded from cell counts.

User-Changeable Fluorescence Optics Modules

Cellometer Vision CBA - User Changeable Optics Module

The Vision CBA Image Cytometry System features two user-changeable fluorescence optics modules. The robust modules are easily removed and replaced. Available modules cover a spectrum from UV to far-red, enabling analysis of a wide range of fluorescent proteins, dyes, and fluorophores.

Optics Module Fluorophores Nucleic Acid Stains Fluorescent Proteins
VB-450-302
Ex: 375 nm
Em: 450 nm
AlexaFluor® 350 DAPI
Hoechst 33342
Hoechst 33258
BFP
CFP
VB-535-402
Ex: 475 nm
Em: 535 nm
Calcein
FITC
AlexaFluor® 488
AO (acridine orange, +DNA)
SYTO®9, SYTO®13
GFP
YFP
VB-595-502
Ex: 525 nm
Em: 595 nm
AlexaFluor® 546
AlexaFluor® 555, Cy3®
PE (R-phycoerythrin)
Rhodamine B
PI (propidium iodide)
EB (ethidium bromide)
SYTOX® Orange
RFP
Ds Red
TdTomato
VB-660-503
Ex: 540 nm
Em: 660 nm
AlexaFluor® 647
7-AAD
Nile Red
PI (propidium iodide)
EB (ethidium bromide)
AO (acridine orange, +RNA)
VB-695-602
Ex: 630 nm
Em: 695 nm
AlexaFluor® 647, Cy5®
APC (allophycocyanin)
SYTOX® Red Crimson
*This table is a partial list of compatible fluorophores, nucleic acid stains, and fluorescent proteins. Please contact Nexcelom technical support regarding compatibility of other reagents.
Sytox, AlexaFluor, and Cy are trademarks of Life Technologies

Trypan Blue Viability

Cellometer Vision CBA - Trypan Blue For Cell Viability

In addition to dual-fluorescence viability, the Vision includes a default assay for cell concentration and % viability for cultured cells stained with trypan blue.

Trypan blue viability is a dye exclusion method that utilizes membrane integrity to identify dead cells. The dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging.

Other bright field stains, such as Methylene Blue and Erythrosine B, can also be analyzed using Cellometer cell counters.

Counted Trypan Blue Bright Field Image

Cellometer Vision CBA - Counted Trypan Blue Bright Field Image

Counted Erythrosin B Bright Field Image

Cellometer Vision CBA - Counted Erythrosin B Bright Field Image

Live cells are circled in green. Dead cells are circled in red.

Fast

Within 30 seconds, the Vision instrument reports:

  • Cell concentration (total, live, and dead)
  • Cell number (total, live, and dead)
  • Mean cell diameter (total, live, and dead)
  • Percent viability

Hepatocyte Analysis

Primary hepatocytes (both fresh and cryo-preserved) are routinely used to test potential drug candidates for toxicity. Seeding with identical numbers of viable cells is important for accurate toxicity experiments. Due to their variable morphology and tendency to clump, hepatocytes can be difficult to count manually or with other automated cell counters. The Cellometer Vision features specialized algorithms for accurate counting of hepatocytes. Dual-staining with AO/PI enables counting of live and dead hepatocytes for automated viability determination.

Cellometer Vision CBA - Hepatocyte Analysis

Bright field image (left) shows the variable morphology of primary hepatocytes. Dual fluorescence image (right) shows counted live cells (circled in green) and counted dead cells (circled in red).

Simple Imaging Method Ideal for Fragile Cells

Hepatocytes are quite fragile. Because the Vision images cells directly from the counting chamber, the shear stress present in flow-based systems (where cells travel through the instrument) is eliminated.

Fast Analysis for Accurate Viability Determination

Because hepatocytes lose viability over time, it is important to complete the analysis very quickly. Staining with the Cellometer AO/PI viability reagent and loading of the counting chamber takes just 1 minute. Analysis with the Cellometer Vision is completed in less than 60 seconds.

Yeast Viability for BioFuel Production with Vision 10x

The Cellometer Vision (10x) Image Cytometry System is optimized for simple, 1-step determination of yeast concentration and viability in messy samples. Fluorescent nuclear staining dyes are used to stain live and dead nucleated cells. Learn more about the AO/PI dual-fluorescence method.

The Cellometer Yeast Viability Kit was specifically developed for dual-fluorescence analysis of yeast in samples containing corn mash. Reagents for viability of yeast in samples containing corn stover, sugar cane, and biomass are also available. Contact Nexcelom technical support for more information.

Cellometer Vision CBA - Bright Field and Fluorescent Images of Yeast

Bright field and fluorescent images of yeast from corn mash. Debris visible in the bright field image (above left) is excluded in the dual-fluorescence image (above right). Only live (circled in green) and dead (circled in red) yeast are counted.

Analysis of Clumpy and Irregular-Shaped Cells

Cellometer Vision CBA - Irregular Shaped Cells Analysis

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells, activated T-cells, and hepatocytes.

  • More than 1,600 different cell lines have been successfully counted using Cellometer Systems including the NCI-60 human cancer cell lines developed by the National Cancer Institute.
Cellometer Vision CBA - Clumpy Cells Analysis

Clumpy Cells

The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown at right).

For clumpy cells with poorly-defined edges, the Vision offers fluorescent counting. Cells can be stained with a nuclear dye, such as acridine orange (AO), then imaged and counted in the fluorescent channel.

Adipocyte Analysis

Nexcelom has designed specialized (PD-300) counting chambers to accommodate large cells, including adipocytes. The controlled chamber depth eliminates potential problems caused by the buoyancy of adipocytes. Imaging directly from the counting chamber eliminates the shear stress present in flow-based systems where cells travel through the instrument, making accurate analysis of fragile adipocytes possible.

Cellometer Vision CBA - Excluded Free Lipid Droplets

Optimized bright field counting parameters or fluorescent stains can be used to exclude free lipid droplets (shown left) from counting results. Optimized settings for adipocyte analysis are saved as a unique cell type

Cellometer Vision CBA - Bright Field Image of Adipocytes

Bright field image of adipocytes

Cellometer Vision CBA - Bright Field Counted Image of Adipocytes

Bright field counted image of adipocytes

Cellometer Vision CBA - Dual Fluorescence Live / Dead Counted Image of Adipocytes

Dual fluorescence live / dead counted image of adipocytes

Exclusion of Debris & Size-Based Counting

Because Cellometer recognizes cells based on size, brightness, and morphology, cellular debris is easily and accurately excluded from counting results.

  • Cell size parameters can be modified to optimize exclusion of debris from results and enhance the accuracy of counting for a wide range of cell sizes.
  • A fluorescent nuclear dye, such as acridine orange, can be used to more easily identify nucleated cells in samples containing debris.
Cellometer Vision CBA - Cellular Debris Excluded from Counting Results

Cell Size Histogram

The Cellometer Vision automatically generates a cell size histogram based on cell diameter.

Cellometer Vision CBA - Cell Size Histogram

Adipocyte cell size histogram showing variation in cell diameter from 44 to 202 microns.

Size-Based Counting

Minimum and maximum cell diameter settings in the Cellometer software can be optimized to count specific cells in a sample. The example below demonstrates counting of mature dendritic cells cultured from PBMCs based on cell diameter.

Cellometer Vision CBA - Size-Based Counting

View, Print, and Save Cell Images and Data Tables

Cellometer Vision CBA - Confirmed Counted Cells

Bright field and fluorescent counted images of macrophages stained with Calcein-AM for detection of metabolically-active cells. Debris and dead cells in the bright field image (left) are not visible in the fluorescent image (right).

View Bright Field Images to check cell morphology.

View Fluorescent Counted Images to:

  • confirm exclusion of debris
  • confirm exclusion of unwanted cell types
  • optimize counting of irregular-shaped cells
  • confirm counting of individual cells within clumps

Cellometer Vision CBA - Automatically Save Raw Images

Automatically Save raw images and data to a secure network location.

Export data to Excel

Export Data to Excel.

Cellometer Vision CBA - Capture Colored Images

Capture Colorized Images with screen-capture software for use in publications and presentations.

Print data

Print images and custom data reports directly from Cellometer software and FCS Express.

Counting Chambers: No Washing or Contamination

Cellometer Vision CBA- Counting Chamber

Cellometer Disposable Counting Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette.

The chamber is inserted into the Cellometer cell counter and the cells are imaged directly from the chamber. This simple sample loading and analysis method is ideal for fragile cells.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

  • Time savings – no washing
  • No risk of cross-contamination
  • Reduced biohazard risk to users
  • Controlled sample volume
  • Controlled chamber depth
  • Direct imaging from chamber
  • Most affordable automated counting consumables

Dedicated On-line and On-site Applications Support

On-site demonstrations

On-site demonstrations allow users to evaluate a critical cell type or cell-based assay prior to making a purchase.

Technical seminars

Technical seminars are a great way to introduce Cellometer cell viability and cell-based assays to multiple users within a lab or organization.

Free on-site installation and training

Free on-site installation and training are available to all Vision and Vision CBA customers.

Web-based support sessions

Web-based support sessions are available to all Cellometer customers at no cost. Nexcelom technical support specialists can assist with creation of new cell types, optimization of counting parameters, and analysis of cell-based assays.

Comprehensive Protocols

Comprehensive Protocols for a growing menu of cell-based assays assist with sample preparation, imaging, and data analysis. Detailed, step-by-step instructions with screen shots enable new users to run Cellometer kits for cell cycle, apoptosis, cell viability, and other assays.

Help

On-Line Help can be accessed directly from the Vision and Vision CBA Instruments. Users can:

  • View training videos
  • View frequently asked questions
  • Download instructions and software updates
  • Submit a Support Ticket directly to Nexcelom

Cellometer Vision vs. Vision CBA: Which System is Right for Me?

The Cellometer Vision and Vision CBA are specifically optimized for concentration and viability analysis of complex cell samples and unique sample types. Unlike the standard Cellometer Vision, the Vision CBA features an additional light source (HSL Module) for enhanced sensitivity and FCS Express 4 Flow software for advanced population analysis based on fluorescence intensity. Assay data is displayed in a table and also as a scatter plot or histogram for simple identification and measurement of unique cell populations. The table below compares Vision and Vision CBA capabilities for a number of popular cell types and cell-based assays.

Application or Cell-Based Assays Cellometer Vision Cellometer Vision CBA
Live/Dead Cell Concentration & Trypan Blue Viability (clean cell samples) X X
Live/Dead Nucleated Cell Concentration & Dual-Fluorescence Viability (samples with and without debris, RBC, platelets; no lysing) X X
Small Cells: Yeast, Algae, Platelets 10x 10x
Hepatocyte and Adipocyte Analysis X X
Cell Cycle: PI X
Apoptosis: Annexin V-FITC/PI, Caspases, Mitochondrial Membrane Potential X X
Autophagy X X
GFP, RFP, YFP, and other fluorescent proteins* X X
Surface Markers: FITC*, PE*, APC* X
Proliferation: CFSE* X
Apoptosis: Chromatin Condensation* X
X = numbers only X = with population plots and histograms
*Enhanced sensitivity of Vision CBA is strongly recommended for this application
*Enhanced sensitivity of Vision CBA may be required if signal is weak

Cellometer Vision Accessories

Cellometer Vision / Vision CBA Fluorescence Optics Modules

User-changeable fluorescence optics modules compatible with the Cellometer Vision and Vision CBA Systems

Catalog # Description Compatible Dyes / Fluorophores* Unit
VB-450-302 Cellometer Vision Optical Module
Excitation / Emission: 375nm/450nm
DAPI, Hoechst 33342 and 33258, BFP, CFP each
VB-535-402 Cellometer Vision Optical Module
Excitation / Emission: 475nm/535nm
Acridine Orange (AO) (+DNA), Fluorescein (FITC), SYTO®9, SYTO®13, Calcein, Fluorescein (FITC), AlexaFluor®488, GFP, YFP each
VB-595-502 Cellometer Vision Optical Module
Excitation / Emission: 525nm/595nm
Propidium Iodide (PI),Ethidium Bromide (EB), SYTOX® Orange, AlexaFluor®546, AlexaFluor®555, Cy3®, R-phycoerythrin (PE), Rhodamine B, Ds Red, RFP, TdTomato each
VB-660-502 Cellometer Vision Optical Module
Excitation / Emission: 540nm/660nm
Propidium Iodide (PI),Ethidium Bromide (EB), Acridine Orange (AO) (+RNA), AlexaFluor®647, 7-AAD, Nile Red each
VB-695-602 Cellometer Vision Optical Module
Excitation / Emission: 630nm/695nm
Allophycocyanin (APC), AlexaFluor®647, Cy5®, SYTOX® Red, Crimson each
*An incomplete listing of popular fluorophores, dyes, and fluorescent proteins that are compatible.Customized optical modules in the UV to far-red range are available. E-mail support@nexcelom.com or call 978-327-5340 for more information.

Software

Software upgrades available for the Cellometer Vision CBA Instrument

Catalog # Description Size Unit
VSL-01 Cellometer Vision Software License for GMP/GLP Support Single-user copy 1 license
FCS-SW4RP-10 Additional single-user copy of FCS Express 4 Flow, RUO Professional Software Single-user copy 1 license

IQ / OQ Validation Package

Installation Qualification (IQ) and Operational Qualification (OQ) package for the Vision instrument.

Service Description Size Unit
Cellometer Vision-IQOQ Counting chambers, reference bead solution, and IQ/OQ validation protocol for the Cellometer Vision 100 counts 1 Set

On-Site Service, Maintenance, and Instrument Warranties

Trained Nexcelom Applications Specialists are available for on-site preventative maintenance, IQ validation, and OQ validation.

Service Description
Preventative Maintenance Available for all Cellometer Systems

Please inquire for scheduling and pricing.

IQ Validation* Available for Auto T4 and Vision Systems

Please inquire for scheduling and pricing.

OQ Validation* Available for Auto T4 and Vision Systems

Please inquire for scheduling and pricing.

Extended Warranty Available for all Cellometer Systems currently under a valid warranty.

Please inquire for pricing.

*IQ/OQ Validation Package must be purchased separately

Models and Specifications for Cellometer Vision CBA Image Cytometer

Cellometer Vision CBA Cellometer Vision
Includes
  • Cellometer Vision HSL Instrument
  • Cellometer Software
  • FCS Express 4 analysis software (2 licenses)
  • Two Fluorescent Optics Modules
  • Power Supply • USB 2.0 Connection Cable
  • Phone/online applications support during set-up
  • Cellometer Vision Instrument
  • Cellometer Software
  • Two Fluorescent Optics Modules
  • Power Supply
  • USB 2.0 Connection Cable
  • Phone/online applications support during set-up
Magnification Selection
  • 5x (ex: most mammalian cells)
  • 10x (ex: yeast, platelets)
  • 5x (ex: most mammalian cells)
  • 10x (ex: yeast, platelets)
Cell Size:

  • 5 – 300* microns (5x)
  • 0.5 – 25 microns (10x)

Conc. Range:

  • 105 – 107 cells/ml (5x)
  • 105 – 107 cells/ml (10x)

Bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.

*Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter

Cell Size:

  • 5 – 300* microns (5x)
  • 0.5 – 25 microns (10x)

Conc. Range:

  • 105 – 107 cells/ml (5x)
  • 105 – 107 cells/ml (10x)

Bright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.

*Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter

Instrument Specification
  • Weight: 25.0 lbs. (11.3 kg)
  • Dimensions: Width: 6.0″ (15.2 cm)
  • Depth: 8.5″ (21.6 cm)
  • Height: 14.0″ (35.6 cm)
  • Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
  • Output to Instrument: 12 VDC, 3.34A
  • Weight: 25.0 lbs. (11.3 kg)
  • Dimensions: Width: 6.0″ (15.2 cm)
  • Depth: 8.5″ (21.6 cm)
  • Height: 14.0″ (35.6 cm)
  • Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
  • Output to Instrument: 12 VDC, 3.34A
PC/Laptop Requirements

(if purchasing Cellometer without PC laptop)
  • Windows 7, 8 or 10
  • 1.6 GHz or higher processor
  • 2GB RAM • USB 2.0 port
  • Display resolution: 1024 x 768 pixels or higher

Intel Mac with Parallels/Windows

  • Windows 7, 8 or 10
  • 1.6 GHz or higher processor
  • 2GB RAM • USB 2.0 port
  • Display resolution: 1024 x 768 pixels or higher

Intel Mac with Parallels/Windows

Available Fluorescent Optics Modules VB-450-302 

Excitation / Emission: 375nm/450nm

Example Fluorophores:

  • DAPI
  • Hoechst 33342 & 33258

VB-535-402

Excitation / Emission: 470nm/535nm

Example Fluorophores:

  • Acridine Orange (+DNA)
  • GFP
  • FITC

VB-595-502

Excitation / Emission: 525nm/595nm

Example Fluorophores:

  • Propidium Iodide (PI)
  • Ethidium Bromide (EB)
  • R-phycoerythrin (PE)

VB-660-503

Excitation / Emission: 540nm/660nm

Example Fluorophores:

  • Propidium Iodide (PI)
  • Ethidium Bromide (EB)
  • 7-AAD

VB-695-602

Excitation / Emission: 630nm/695nm

Example Fluorophores:

  • Allophycocyanin (APC)
  • Cy5®
VB-450-302 

Excitation / Emission: 375nm/450nm

Example Fluorophores:

  • DAPI
  • Hoechst 33342 & 33258

VB-535-402

Excitation / Emission: 470nm/535nm

Example Fluorophores:

  • Acridine Orange (+DNA)
  • GFP
  • FITC

VB-595-502

Excitation / Emission: 525nm/595nm

Example Fluorophores:

  • Propidium Iodide (PI)
  • Ethidium Bromide (EB)
  • R-phycoerythrin (PE)

VB-660-503

Excitation / Emission: 540nm/660nm

Example Fluorophores:

  • Propidium Iodide (PI)
  • Ethidium Bromide (EB)
  • 7-AAD

VB-695-602

Excitation / Emission: 630nm/695nm

Example Fluorophores:

  • Allophycocyanin (APC)
  • Cy5®

For customized optical modules, email support@nexcelom.com or call: 978-327-5340.

Get Flow Results without Flow Cytometry

Incorporation of FCS Express Software into the Vision CBA Analysis System has enabled users to conduct image-based analysis of cell-based assays requiring enhanced gating for the most accurate, reliable, and reproducible results. Side-by-side comparison of results for apoptosis, cell cycle, mitochondrial potential, autophagy, ABC transporter assays, aggresome detection, CD4+ / CD8+ immunophenotyping, and other assays has shown Cellometer image-based analysis results to be comparable to flow cytometry.

Flow core labs can utilize the Vision CBA instrument to pre-screen samples prior to sorting and flow cytometry analysis and to run 1-color and 2-color assays for cell cycle, apoptosis, GFP transfection, mitochondrial potential, and more … freeing up their flow system for the more complex studies.

Individual researchers performing simple 1-color and 2-color analysis appreciate the simplicity and accessibility of the Vision CBA. The ability to analyze samples right away, using only 20µl of sample, enables researchers to accelerate and expand their experiments. Researchers can analyze a number of time-points, rather than one endpoint result. The FCS Express Software offers straightforward data analysis procedures and outputs familiar to many flow users.

FCS Express Software Features

  • Colorized scatter plots and histograms
  • More accurate gating for enhanced sensitivity
  • Pre-set, optimized data layouts
  • Simple import and creation of data layouts for new assays
  • Flow-like data presentation
  • Multi-sample data formatting
  • Convenient Export to Excel

Customized Data Layouts

As shown in the Nexcelom data layout to the left, FCS Express Software plots data based on fluorescence intensity and default gating parameters. The data table displays the cell percentage and cell concentration for each phase of the cell cycle. Gating is manually optimized by dragging the gating bars in the histogram. Cell percentages and concentrations are instantly updated in the associated data table.

For analysis of Apoptosis with Annexin V-FITC / PI, FCS Express Software generates colorized scatter plots indicating live, apoptotic and dead cells with an associated data table indicating the % of cells and cell concentration in each category.

If you do not currently have FCS Express but would like to see data layout examples, you can download the following PDF samples:

FCS Express 4 Software plots data based on fluorescence intensity and default gating parameters.

Express 4 Software generates colorized scatter plots

The Cellometer Vision CBA has removed the variances regarding human error and subjectivity testing essentially overnight from our operations!

Joseph Thomas, Mass Bay Brewing Company

The Cellometer Vision CBA Image Cytometer is a great machine! It gives very precise results in a very short time, and only uses a small amount of sample, which makes it very efficient for research purposes.

Nelson Franqui, Darlington Building

Using the Cellometer Vision CBA has saved me so much time! I am able to get consistent viability data with much less time spent on sample preparation. It has also been great to complete cell cycle analysis without using FACS.

Sarah Burkhart, Florida State University College of Medicine
I love the ability to rapidly count mouse white blood cells and red blood cells without the hassle of needing to lyse the red blood cells with the Cellometer Vision CBA! The AO/PI solution makes this process a breeze.

The Cellometer Vision CBA has removed the variances regarding human error and subjectivity testing essentially overnight from our operations! – Joseph Thomas, Mass Bay Brewing Company

I frequently use the Cellometer Vision CBA and I really like it!

The Cellometer Vision CBA makes my life so much easier! It makes cell counting a breeze! It is quick and very easy to use. You can count your cells with a turn of a knob and with a click of a button!

The Cellometer Vision CBA works great and is really efficient. Earlier, I used to work with the Cellometer Auto T4 but then switched to the Vision CBA. With the Cellometer, I consumed less time and it helps me get research work done effectively!

The Cellometer Vision CBA definitely saved me from spending hours on the microscope counting cells and it spared me the need to understand complicated FACS analysis.

The Cellometer Vision CBA is user-friendly, making it easy to understand the software and it delivers fast results! – Lyanne Suarez, University of Puerto Rico Aguadilla

The Cellometer Vision CBA Image Cytometer is a great machine! It gives very precise results in a very short time, and only uses a small amount of sample, which makes it very efficient for research purposes. – Nelson Franqui, Darlington Building

We have the Cellometer Auto T4, Cellometer Auto 2000 and Cellometer Vision CBA. Our Cellometers are reliable and every time I call for a product or technical service, I have spoken to a helpful and knowledgeable person.

We use the Cellometer Vision in our lab. We love this machine! It always gives us quick and clear cell counts!

The Cellometer Vision CBA instrument quickly counts cells and measures viability. The instrument is quick and easy to use. Counting cells using the Cellometer is much easier than using a hemacytometer!

The ease at which I can count bone marrow samples without Red Cell Lysis buffer to get total nucleated cell counts quickly and accurately with the Cellometer Vision CBA has allowed our research to expand drastically! – Daniel Kuebler, Franciscan University

The Cellometer Vision CBA is a perfect cross between the Bio-Rad cell counter and regular flow cytometer. We are just beginning to use this method of platelet counting and we are looking forward to continuing to use this instrument! – Shauna-Kay Spencer, University of Mississippi Medical Center

Our experience using the Cellometer Vision CBA has been great! It is so useful. We recommend the instrument due to convenience and speed of reading.

The Cellometer Vision CBA has helped me complete my experiments much faster! The instrument allows for rapid cell counts and even has a tool that can help calculate the cell concentration in no time. I love the counting chambers that are easy to load as well!

The Cellometer Vision CBA makes counting cells so much easier and less labor intensive!

The Cellometer Vision CBA has greatly streamlined our cell counting activities and its versatility has enabled us to customize many unique counting protocols for different cell lines. All in all, well worth the investment!

The Cellometer Vision CBA is very helpful with cell viability and plating. It really saves time!

The Cellometer Vision CBA has been so useful because now we do not waste a lot of time counting cells. Also, proliferation and viability experiments are too easy to analyze using this Cellometer! – Ana Milena Reyes Ramos, University of Puerto Rico – Mayaguez

The Cellometer Vision with your specialized chlorophyll fluorescein module will save us countless hours of microscope work by providing a reliable viability count of cells in a matter of minutes. We love it!

The Cellometer Vision CBA has helped us optimize a lot of our experiments. Not only is it fast and reliable, but it is easy to modify and create assays within the program. I would recommend it to others!

We were originally using the Cellometer Auto T4 system for cell count and viability and it made our cell counts quicker than when using a hemacytometer! But now we have upgraded to the Cellometer Vision CBA, and the focusing time is almost on par with a conventional microscope and with the extremely useful fluorescent cell counting. This system will probably outlast the computer we use to operate it! -Reinaldo Agostini, University of Puerto Rico

Before our lab moved, we were utilizing the Cellometer Vision CBA from Nexcelom. The capabilities of this machine are amazing. The Propidium Iodide (PI) staining for cell cycle analysis was the function we used most frequently. This helps remove the need for a large volume of cells like flow would require. Since our cell lines were going into arrest after our manipulations, we were unable to generate large quantities of cells for traditional flow. The Vision CBA fit perfectly for our needs. Now, we use the Cellometer Mini due to financial constraints when our lab moved. It is still a great instrument, but if given the chance, I’d 100% without a doubt go back to the Cellometer Vision CBA. There are too many things it can do to pass it up! -Amy Rosado, Nemours Children’s Hospital

The Cellometer Vision CBA has provided highly trustworthy and fast results using the powerful algorithms for automated image-based cell counts and flow-like analysis. Plus, there is no need to clean up the instrument and it is a very user-friendly interface. I highly recommend it!

Our Cellometer Vision CBA allows us to easily and accurately perform cell-based assays for our drug resistance experiments!

The Cellometer Vision CBA is by far the best product we have used in our lab. In such little time, this small machine can capture images, analyze data and upload your data into a neat powerpoint that can be used immediately. We have used this machine for apoptosis assays and cell cycle assays and it has not disappointed!

The Cellometer Vision is a great product! The instrument is quick and easy to learn to use, and the software is very user-friendly. We have used our Cellometer to obtain data that we would normally have to use the flow cytometry facilities to get. The machine has made a number of data types both inexpensive and quick to obtain. In addition to the product itself, the customer service we have received is excellent – only adding to the quality of the product!

Using the Cellometer Vision CBA has saved me so much time! I am able to get consistent viability data with much less time spent on sample preparation. It has also been great to complete cell cycle analysis without using FACS. – Sarah Burkhart, Florida State University College of Medicine- Biomedical Sciences