///Cellometer Auto 2000 Cell Viability Counter
Cellometer Auto 2000 Cell Viability Counter2018-10-26T18:44:43+00:00

Cellometer Auto 2000 Cell Viability Counter

Touchscreen fluorescent automated cell counter for primary samples

Why Auto 2000?

  1. Primary Cell Analysis: PBMCs, Stem Cells, and more
  2. No Interference from Red Blood Cells, Platelets, or Debris
  3. Analysis of Clumpy and Irregular-shaped Cells

I like the cell images with AO/PI dye. When a cell line didn’t have GFP protein just looking at the images I could tell that there was something wrong with it. I have compared it [Cellometer Auto 2000] with a hemacytometer and they are comparable while Vi-Cell was not.

Elstar Therapeutics

We have been counting PBMCs on a coulter counter, and once that broke, a manual hemocytometer. The Auto 2000 is faster, easier, and more effective than either method, to say nothing of the reduced hassle of cleaning a hemocytometer or constantly flushing and priming an aperture.

Rutgers New Jersey Medical School

The Auto 2000 is extremely useful for our laboratory. We use one a lot of times during our day. Cellometer helps do experiments much faster than before.

Brigham and Women's Department of Pathology

This instrument is very helpful in our lab – we use it for AO/PI, trypan, and bright field staining for at least 5 different cell types and we are confident in the accuracy of the [Cellometer] Auto 2000.

Happy Customer

Ease of use is probably the most dominating feature [of the Cellometer Auto 2000]. Backed by a very supportive staff, using instruments or services through Nexcelom has been a pleasure.

Edgard Raymond
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Introduction to the Cellometer Auto 2000 Cell Viability Counter

Simple, User-friendly Procedure

Simple, User-friendly Procedure for Cellometer Auto 2000

 Pipette 20µl  Insert Slide  Select Assay & Click Count  View Results in 30 seconds!

Simple, Automated Cell Counting in 30 Seconds

The Auto 2000 utilizes bright field imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

Results in less than 30 seconds
Load Sample, View Image, Count Cells, and Obtain Results in < 30 seconds

The Auto 2000 Allows Users to:

  • Increase throughput
  • Increase accuracy
  • Improve consistency
  • Ensure all data is correctly captured
  • Count difficult cells (clumpy, irregular-shaped)
  • Eliminate judgment errors, miscounts, interference from red blood cells and user-to-user variability

Primary Cell Analysis: PBMCs, Stem Cells, and more

The Cellometer Auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including:

  • Nucleated Cells for Transplantation
  • PBMCs for Immunology
  • Splenocytes for Vaccine Development
  • Stem Cells for Cellular Therapy
  • Tumor Cell Suspensions for Oncology

Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both ‘messy’ and ‘clean’ samples enables the Auto 2000 to evaluate samples at a variety of points throughout sample processing – from initial collection to separation, to cryopreservation.

The Cellometer Auto 2000 features one-touch assays for analysis of a wide range of primary samples, including:

Nucleated Cells from Whole Blood

Nucleated Cells from Whole Blood using Cellometer Auto 2000

Stem Cells

Stem Cells, image from Cellometer Auto 2000

PBMCs / Splenocytes following Separation

PBMCs / Splenocytes following Separation using Cellometer Auto 2000

Tumor and Tissue Cell Suspension

Tumor and Tissue Cell Suspension using Cellometer Auto 2000

No Interference from Red Blood Cells, Platelets, or Debris

The dual-fluorescence AO/PI method utilizes nuclear staining dyes that bind to nucleic acids in the cell nucleus. Because most mature mammalian red blood cells do not contain nuclei, only live and dead mononuclear cells produce a fluorescent signal. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step. Red blood cells, platelets, and debris are not counted in the fluorescent channels.

The advantage of fluorescent counting for primary cells

These images (right) demonstrate the advantage of fluorescent counting for primary cells. The bright field image shows the combination of nucleated cells, red blood cells, and platelets present in the sample. Only the live and dead nucleated cells are visualized and counted in the green and red fluorescent channels.

Sample Measurement Total nucleated All RBC % RBC n
PBMC+RBC Mean

CV

1.26E+07

6.2%

1.39E+07

8.8%

1.23E+06 8.9% 10
PBMC+1/2RBC Mean

CV

1.21E+07

4.8%

1.27E+07

5.4%

5.82E+05 4.6% 10
PBMC+1/4RBC Mean

CV

1.22E+07

7.9%

1.24E+07

7.3%

2.27E+05 1.8% 10

Fresh human PBMCs (peripheral blood mononuclear cells) were spiked with varying amounts of RBCs (red blood cells.) All cells (nucleated + RBC) were counted in the bright field channel. Nucleated cells were then counted in the green fluorescent channel. Varying amounts of RBCs (1.8%, 4.6%, and 8.9%) did not affect the nucleated cell count.

Several red blood cells are indicated in the bright field image (above, left). The red blood cells are not visible in the fluorescent image (above, right) detecting cells stained with nuclear staining dye.

Analysis of Clumpy and Irregular-shaped Cells

Including NCI-60 and clumpy MCF-7 Cells

NCI-60 is a group of 59 human cancer cell lines (originally 60) developed by the National Cancer Institute for screening purposes.

  • 57% of the NCI-60 cell lines are clumpy, contain debris, or display large variations in cell shape or size
  • All 59 NCI-60 cell lines have been successfully validated on the Cellometer Cell Counter

All 40 of the NCI Comprehensive Cancer Centers use Cellometer Cell Counters.

Clumpy Cells

The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown above).

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells and activated T-cells.

User-Friendly Touch Screen

The user-friendly touch screen displays pre-optimized assays for common cell types. User-specific protocols can be easily created and saved to the menu. The simple, pre-optimized assays make the instrument easy to operate and simplify training for new users.

With the click of a button, researchers can select a new assay with saved counting parameters, making the instrument ideal for labs testing a variety of cell types. Auto-save options allow users to quickly test a series of samples.

Live / Dead Nucleated Cell Counts using Dual-Fluorescence

Green fluorescent live cell image

Red fluorescent dead cell image

Why Dual-Fluorescence?

Because bright field cell counting does not differentiate nucleated from non-nucleated cells and trypan blue staining is not as easy to detect as fluorescent staining, dual-color fluorescence is strongly recommended for accurate viability analysis for primary cells. The Auto 2000 is equipped with standard assays for dual-fluorescence analysis of primary cells stained with AO/PI.

The AO/PI Method

Acridine Orange, AO, is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence. Propidium iodide, PI, can only enter dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.

Cell Images for Data Verification

No two cells are the same.

With the Auto 2000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.

Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly. Bright field counted images can be viewed for basic cell counting and trypan blue viability.

Fluorescent counted images indicating counted live and dead nucleated cells can be viewed for dual-fluorescence primary cell viability assays.

Users can confirm that:

  • cells are counted correctly, based on size and shape
  • cells within clumps are being counted individually
  • red blood cells, platelets, and debris are being excluded from results

The bright field image confirms that individual cells within pairs are being counted and smaller debris is not being counted. In the combined fluorescent counted image, live counted cells are circled in green. Dead counted cells are circled in red.

  • Cell images can be archived and exported for use in publications and presentations.
  • Saved images can be re-counted using default or user-optimized analysis settings

Cell Size Analysis & Size-based Counting

The Auto 2000 Cell Counter Automatically generates a cell size histogram based on cell diameter.

Because Cellometer generates individual cell size measurements, multiple samples can be overlaid on one histogram enabling analysis of the change in cell diameter over time.

For experiments involving SF9 or SF21 cells, saved images can be viewed and re-counted to analyze changes in morphology over time.

The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.

This example demonstrates counting of mature dendritic cells cultured from PBMCs based on cell diameter.

10x Faster than Manual Counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 2000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in just 30 seconds.

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps

Eliminate Judgment Errors

Eliminate interference from RBCs

Eliminate Recording & Calculation Errors

Reduce Counting Time … Run More Experiments

Cellometer Precision

The Cellometer Auto 2000 offers excellent reproducibility, with a %CV (Coefficient of Variation) of <10% for fluorescent concentration and viability measurements. The data (below) is based on four preparations of Jurkat cells stained with propidium iodide, a fluorescent nuclear-staining dye.

Sample N Value Average Live Cell Concentration % Viability CV of Concentration CV of Viability
A 4 4.20E+06 91.1 10% 2%
B 4 1.06E+06 22.7 7% 1%
C 4 3.27E+06 57.5 7% 7%

Imaging / Counting Chambers: No Washing or Contamination

Cellometer Disposable Imaging Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

Time savings – no washing

No risk of cross-contamination

Reduced biohazard risk to users

Controlled sample volume

Large-depth chambers for large cells

Most affordable automated counting consumables

Auto-Save Data and Cell Images

The Auto 2000 allows users to generate specific folders to which data reports and images can be auto-saved. Using the auto-save feature ensures all data is accurately captured in the correct location. It also enables users to test a series of samples very quickly.

Users can configure the Auto 2000 to request a new sample name for each sample that is imaged and counted or apply auto-numbering to individual samples in a series to further reduce analysis time. Users can also apply a time stamp to individual data files.

Dedicated On-line and On-site Applications Support

Experienced Nexcelom Technical Support Specialists are available from 8:30am to 5:00pm EST for phone and on-line support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of the Auto 2000 Cell Viability Counter

The help button at the bottom right of the Cellometer Auto 2000 software screen gives users instant access to:

  • Software features and instructions
  • On-line tutorials and training videos
  • Submission of a Support Ticket

All Nexcelom Applications Specialists are 100% focused on image-based cell concentration & viability and cell-based assays using Cellometer Image Cytometry.

Nexcelom Field-based Applications Specialists are also available for:

  • On-site demonstrations
  • Training
  • Troubleshooting
  • Technical Seminars

Applications for Cellometer Auto 2000 Cell Viability Counter

Adipocytes

Adipocytes

Automatically measure cell size of freshly isolated adipocytes and plot size histogram. DNA staining fluorescence dyes are used to identify cells from lipid droplets. »

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Viability and Necrosis

Cell Viability and Necrosis

Cell viability is performed using various fluorescent membrane exclusion dyes, such as PI, EB, 7AAD, and others. This assay is performed by enumerating cells in captured bright-field and fluorescent images. And Necrotic cells are detected using propidium iodide. »

Blood Samples

TNC Concentration & Viability for Clinical (Blood) Samples

Analyze fresh and processed blood and bone marrow samples without lysing: no interference from RBCs. »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

GFP Transfection Efficiency

Quantitative Measurement of GFP Transfection

Rapidly identify fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration, size and determine the GFP transfection automatically. »

Immunology

Immunology Research

Automatically quantify cell viability and concentration for a variety of immunologically relevant samples such as: bone marrow, cord blood, slpenocytes, lymphocytes, isolated mononuclear cells, tumor digests, murine samples, and others. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

PBMC

Peripheral Blood Mononuclear Cells (PBMC)

Automatically measure live cell concentration and viability without lysing red blood cells for consistent results from patient samples. Other cells include splenocytes and bone marrow. »

Platelets

Platelets

Automatically measure mouse and human platelet concentration using brightfield only method. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

WBSc in Whole Blood

WBCs in Whole Blood

Automatically measure nucleated cell concentration without lysing red blood cells using nuclear staining dyes (AO), for human and mouse blood. »

Stem Cells, Primary Cells, Cell Line

The results indicate the accuracy of the Cellometer Auto 2000 instrument in assessing the viability of Jurkat cells using AO/PI for cell viability. Four measurements were performed for each sample. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring cell concentration and viability of mammalian cells.

Stem Cells, Primary Cells, Cell LineFigure 2: Chart of the % viable cells at 3 different live/dead mixture ratios. N=4 per measurement. The error bars correspond to the standard deviation for each measurement.
Sample N Value Average Live Cell Concentration % Viability CV of Concentration CV of Viability
A 4 4.10E+06 78 1% 2%
B 4 0.0025E+06 0.6 N/A N/A
C 4 2.48E+06 36 4% 7%
Figure 1: Table of results for AO/PI viability using the Cellometer Auto 2000.

Cell Line Viability using Propidium Iodide Only

The results indicate the accuracy of the Cellometer Auto 2000 instrument in assessing the viability of Jurkat cells using PI for cell viability. Four measurements were performed for each sample. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring cell concentration and viability of mammalian cells.

Cell Line Viability using Propidium Iodide Only
Figure 2: Chart of the % viable cells at 3 different live/dead mixture ratios. N=4 per measurement. The error bars correspond to the standard deviation for each measurement.
Sample N Value Average Live Cell Concentration % Viability CV of Concentration CV of Viability
A 4 4.20E+06 91.1 10% 2%
B 4 1.06E+06 22.7 7% 1%
C 4 3.27E+06 57.5 7% 7%
Figure 1: Table of results for cell viability using PI only.

Cell Line Total Cell Concentration

The results indicate the accuracy of the Cellometer Auto 2000 instrument cell concentration. Serial dilutions (2-, 4-, and 8-fold) were prepared using PBS and Jurkat cells. Four measurements were performed for each sample and the averages were calculated and plotted. A linear correlation between the concentration measurements and 1/dilution factor was observed. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring cell concentration.

Cell Line Total Cell Concenration
Figure 2: Jurkat cell concentrations at various sample dilution factors. N=4. Error bars correspond to the Standard Deviations for cell concentration.
Sample Dilution Factor Average Cell Concentration CV of Concentration Standard Deviation N Value
A 1 5.80E+06 1% 4.8E+04 4
B 2 3.39E+06 2% 8.12E+04 4
C 4 1.63E+06 6% 9.54E+04 4
D 8 8.27E+05 6% 5.33E+04 4
Figure 1: Table of results for total cell concentration for the Cellometer Auto 2000 instrument.

Low Concentration of Cells using Acridine Orange

The results indicate the accuracy of the Cellometer Auto 2000 instrument when counting Jurkat cells at very low concentrations (between 5-7E+04 cells/ml). AO was used to measure the concentration of cells in the F1 channel. Results from 4 measurements were plotted. The CV values are very close, which indicates the consistency of the measurements. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring at low cell concentration.

Low Concentration of Cells using Acridine Orange
Figure 2: Graph of Concentration for 4 different measurements using the Low Concentration of Cells Assay and AO on the Cellometer Auto 2000.
Low Concentration AO F1 Concentration
Sample 1 5.91E+04
Sample 2 6.40E+04
Sample 3 6.58E+04
Sample 4 6.90E+04
Average 6.45E+04
STDEV 4.14E+03
CV 6%
Figure 1: Table of results from the Low Concentration of Cells assay.

Performance of the Cellometer Auto 2000 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Auto 2000
Average 1.03E+06 1.01E+06
STDEV 6.60E+04 8.87E+04
%CV 6.4% 8.8%

5 µm beads

N = 20 Hemacytometer Cellometer Auto 2000
Average 1.07E+06 1.03E+06
STDEV 5.90E+04 4.30E+04
%CV 5.5% 4.2%

FAQs about the Cellometer Auto 2000 Cell Viability Counter

Cellometer Auto 2000 has no liquid handling fluidics system. Therefore, cleaning related maintenance is eliminated completely. Optical light sources in Auto2000 are light emitting diodes, with standard lifetime of more than 10,000 hours. Therefore minimum maintenance is required for optics either. Some customers purchase yearly preventative maintenance contract from Nexcelom. We also offer extended warranty. Costs of the services vary.
We will issue a RMA for the unit to be shipped back to Nexcelom for repair. Our standard turn-around time for repair in USA is less than one week after receiving the instrument.
Single board computer running Windows embedded operating system and touch screen.

Imaging capability includes bright field and two fluorescence channels with excitation / emission of 470nm/535 nm and 540nm/635 nm, respectively.

We release software based on customer needs.

No. The most complicated primary hepatocytes are measured using special software algorithm available only with Cellometer Vision.

Specifications for Cellometer Auto 2000 Cell Viability Counter

Cellometer Auto 2000
Includes • Cellometer Auto 2000 Instrument with integrated computer and
touch-screen and dual-fluorescent optics
• Cellometer Software
• Two USB 2.0 ports
• Power Supply
• Ethernet port
• Phone/online applications support during set-up
Imaging Performance Cell Size: 5 – 300* microns
Conc. Range: 105 – 107 cells/mlBright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
*Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter
Instrument Specifications Weight: 26 lbs. (11.8 kg)
Dimensions: Width: 11.1″ (28.3 cm)
Depth: 12.8″ (32.4 cm)
Height: 13.4″ (34.0 cm)Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.5A
Output to Instrument: 12 VDC, 7.5A
Display 10.4″ touch screen
Fluorescent Optics Excitation / Emission: 470 nm/535 nm
Example Fluorophores:
• Acridine Orange (+DNA)
• Calcein AM
• CFDA
• SYTO® 9
• SYTO® 13Excitation / Emission: 540 nm/605 nm
Example Fluorophores:
• Propidium Iodide
• Ethidium Bromide

We absolutely love the Cellometer Auto 2000! The counting is consistent, the purity check that it does for us is amazing. Thank you Nexcelom for making cell counting easier, for scientists/researchers everywhere!

Tinisha McDonald, City of Hope

The Cellometer Auto 2000 is a great counter! It is designed for simple, accurate analysis of nucleated cells, including PBMCS, splenocytes, and stem cells, in peripheral blood, cord blood, bone marrow and other heterogeneous samples.

Ling Li, City of Hope

The Cellometer Auto 2000 is pretty straightforward in terms of its use. It has saved a lot of time from counting cells with hemacytometer. It doesn’t take much space either!

Jiayuan Shinkle, The Jackson Laboratory

The Cellometer Auto 2000 is great for counting AML cells. The cell count is more accurate than Trypan Blue and gives a count for live and dead cells. It has really helped increase consistency in my experiments!

We absolutely love the Cellometer Auto 2000! The counting is consistent, the purity check that it does for us is amazing. Thank you Nexcelom for making cell counting easier, for scientists/researchers everywhere! – Tinisha McDonald, City of Hope

The Cellometer Auto 2000 offers us several quick and easy ways to compare count and viability to more manual methods and other automated methods!

The Cellometer Auto 2000 saves us so much time in daily lab work by eliminating manual cell counts after each passage or thawing. The results are both accurate and reproducible. We use it every single day in our lab!

The Cellometer Auto 2000 is a great counter! It is designed for simple, accurate analysis of nucleated cells, including PBMCS, splenocytes, and stem cells, in peripheral blood, cord blood, bone marrow and other heterogeneous samples. – Ling Li, City of Hope

The Cellometer Auto 2000 is pretty straightforward in terms of its use. It has saved a lot of time from counting cells with hemacytometer. It doesn’t take much space either! – Jiayuan Shinkle, The Jackson Laboratory

I’ve been using a Nexcelom Cellometer for years. When I moved to a different company about 6 months ago, I bought a Cellometer Auto 2000 for my lab and my lab mates love it! I work mostly on human samples and counting using the Cellometer with AOPI is way more accurate than the Vi-Cell or any other counting instrument I’ve used! – Candy Garcia, Ideaya Biosciences

I like the Cellometer Auto 2000 cell counter! It makes it very simple to count primary cells. I have used this counter over the last 7 years, so I can’t imagine any other counters replacing this!

The Nexcelom Cellometer Auto 2000 has been a great tool in determining cell count and viability. When doing cell culture of primary cells it is important to know what you’re dealing with in a timely manner so you can plate the appropriate cell density. The Auto 2000 is a good tool for making this process fast, efficient and accurate. It is a vast improvement over using Trypan Blue and gives results that we can trust!

The Cellometer Auto 2000 is an easy to use, which leads to enhanced productivity. In addition, the system is very amenable to electronic documentation of counts. – Toai Nguyen, Davinci Biosciences, LLC

The Cellometer Auto 2000 is very easy to operate and really helpful for us to count cells! It looks very nice and is not very thick for handling. The two-sided film slides are my favorite, because I don’t need to worry about the contamination of hands or my bench!

The Cellometer Auto 2000 reduces time and we are now able to quickly estimate transfection efficiency. Excellent technical support to help write project specific programs. – Casey Bucan, Waisman Biomanufacturing

The Cellometer Auto 2000 has allowed me to streamline a great deal of my work on cancer drug screening by providing a fast, accurate way to quantity cells with its fluorescent viability platform for cytotoxicity assays.

We have been using the Cellometer Auto 2000 here in our lab for over a year and it has been such a great addition to the lab. Out lab processes PBMCs, counts them and then places them into a 2 week in-vitro stimulation culture in the presence of peptide pools. Two weeks later we harvest the cells, count them and then run them in both an Elispot and a Cytokine stimulation assays. The use of the Auto 2000 cell counter has made the counting of cells much easier in much less time. – Natalia Jun, Human Longevity, Inc.

The Cellometer Auto 2000 can differentiate between live and dead cells which was not possible for us prior to this cell counter. It gives us viability and recovery is easy to calculate. It is very user friendly and we can always save the data on our computer once the counting is done! – Mukesh Mudgal, Florida International University

The Cellometer Auto 2000 works well for us!

We use the Cellometer Auto 2000 routinely for cell death and cell viability assays with AOPI.

The Cellometer Auto 2000 is easy to use and provides accurate counting. Everyone in the lab loves it! It is a great product overall!

My favorite aspect of the Cellometer Auto 2000 from Nexcelom is the ability to view the cells and count them. You can tell right away how your cell density is and if the cell suspension needs to be diluted.

With the Cellometer Auto 2000 it is so easy to count cells! And it uses far less sample than the Nucleocounter without needing to dilute sample!

The Cellometer Auto 2000 is easy to operate and has eliminated the excess time spent on manually counting cell numbers for the large volume of patient samples we receive in the lab. It provides improved counting accuracy and viability information on cells counted and also gives us the ability to refer back to previously counted cell samples as needed.

I’ve been using the Cellometer Auto 2000 with the AOPI premade dye Nexcelom manufactures for the past year. It has been great for my lab group, we would be lost without it!

The Cellometer Auto 2000 is fast and easy to use system. Only 20 uL of cells are needed for each count, which is essential for some precious samples like primary cells.

The Cellometer Auto 2000 requires only a small sample volume and counts cells accurately in a really short time. It allows counting of multiple cell samples and is easy to use for a lot of different cell types.

We have two Nexcelom cell counters in our lab, a Cellometer Auto T4 and Cellometer Auto 2000. Both cell counters have saved us critical time and are very easy to use! The Cellometer Auto 2000 has been invaluable at helping up with difficult-to-count cells, and even with developing new assays. Both are indispensable pieces of equipment in our lab. After trying our Auto 2000, another lab in our building is purchasing one for their own use.

The Cellometer Auto 2000 from Nexcelom has saved me a great deal of time and relieved the stress and uncertainty of using a hemacytometer to count recovered cells from our animal studies. Nexcelom employees are very kind and helpful, the service was very fast and easy to order from, as well as the technical help in using the instrument! – Tina Yaskell, Forsyth Institute

We have the Cellometer Auto T4, Cellometer Auto 2000 and Cellometer Vision CBA. Our Cellometers are reliable and every time I call for a product or technical service, I have spoken to a helpful and knowledgeable person.

Our Applications Scientist helped establish mammalian cell counting protocols for the Cellometer X2 as well as the Cellometer Auto 2000. Both instruments have made cell counting amazingly simple and accurate for live dead cells. We appreciate the training!

The Cellometer Auto 2000 is easy to use. Just choose programs for your cells and focus. It is much faster and accurate than hemacytometer!

I use the Cellometer Auto 2000 in all my experiments! It works like a champ! It saves me so much time. Our entire department loves this Cellometer!

Our lab began using the Cellometer Auto 2000 with the SD100 counting chambers and it has saved us so much time in our experiments! We recently published a paper in Ocotarget citing the Cellometer Auto 2000 to give credit for this fantastic resource: “DNMT3B overexpression contributes to aberrant DNA methylation and MYC-driven tumor maintenance in T-ALL and Burkitt’s lymphoma.”

The Cellometer Auto 2000 is very convenient machine, the resolution is great for the cell count. Live and dead cells can be distinguished very clearly.

The Cellometer Auto 2000 is very reliable, easy to use, and has saved a significant amount of time while providing accurate cell counts!

The Cellometer Auto 2000 is amazingly fast and effective! It has saved me lots of time compared to counting cells using hemacytometer. I would recommend this Cellometer to all my friends and colleagues in other labs!

We currently have four Cellometer Auto 2000 counters operational in my laboratory. These counters are used multiple times daily. We count primary non-human primate lymphocytes which are MUCH smaller than human cells. With proper settings, these counters are so accurate and user friendly that it makes this daunting task so easy. I have been in the biotechnology field for 40+ years and these counters are by far the best and most accurate that I have ever used. Nexcelom service is outstanding, providing us with professional and courteous service and rapid response time! – Vicky Coalter, Leidos Biomedical

The Cellometer Auto 2000 is compact, easy to handle and the software is user friendly! It saves us a lot of time compared to counting on the microscope.

We really like our Cellometer Auto 2000! The faster, move accurate cell counts have improved our data and analysis! – Leila Mulder, Colorado State University

We love using our Cellometer Auto 2000 with the live/dead assay to determine viability! – Steven Frisch, West Virginia University

The Cellometer Auto 2000 from Nexcelom is very robust and useful for counting PBMCs. I have used Cedex and Vi-Cell in the past, which took a lot of time while waiting for samples to be counted. With the Auto 2000, my cells don’t need to sit in a tube while waiting for cell count results!

I have just joined the Immune Monitoring lab at Human Longevity and they have the Cellometer Auto 2000and it is amazing! It cuts cell counting from about 30 minutes down to about 2 minutes or so! This really speeds up the down stream applications! I have used it for both primary and immune cells with equal success and reliability. – Cheryl Andreoli, Human Longevity, Inc

We love the Cellometer Auto 2000! It is very effective, convenient and saves my time! It is very easy to use with the touch screen!

The Cellometer Auto 2000 gives very reliable cell counts, much superior to the competition! – Satinder Dahiya, CHOP

We have been using the Cellometer Auto 2000 to count splenocyte samples without RBC lysis. The Cellometer Auto 2000 and the AOPI Viastain gives us the ability to get an accurate count of our live splenocytes while excluding the red blood cells.

The Cellometer Auto 2000 RULES! Whether I have one sample or 50 samples to count, it is fast and accurate. Almost no matter what your cell concentration, the Auto 2000 will give you the concentration. And not only do you get live cell count, you also get percent viability and cell diameter. I use the Auto 2000 every time I count! – Amy Coddington, Kyowa Kirin Research

The Cellometer Auto 2000 has changed the way we count cells. It saves time and takes away worries about inaccuracy! We can do a lot more in a day because of this machine.

The Cellometer Auto 2000 has a beautiful interface that is easy to use. We process whole blood, and our viability counts have gone up now that the RBCs are excluded.

The Cellometer Auto 2000 is very reliable and easy to use! Counting cells is much simpler with this instrument!

The Cellometer Auto 2000 has provided a robust method for counting cell samples without tech-tech variability. It’s great! – Victoria Groysberg, UCLA Health

The Cellometer Auto 2000 counter has been an incredible addition to our workflow at UCLA Immunogenetics Center. We routinely count PBMCs and cryopreserve them at a specific number per vial. Since adding the Auto 2000 to the lab, our samples have become more reliable in terms of viability and accuracy of counts. I would highly recommend this to any biorepository lab! – Tiffany Sidwell, UCLA

The Cellometer Auto 2000 makes it easier to get an accurate count on my cells compared to flow cytometry. I am comfortable with it and trust the data that I obtain!

The Cellometer Auto 2000 has changed the way my lab works! It is a streamlined workflow and is extremely user friendly!

Our Cellometer Auto 2000 has increased the speed and ease of cell counting. Using it with the AOPI dye has made our cell count and viability measurements much more consistent and accurate than Trypan Blue for morphologically varied tissue homogenates (e.g. mouse small intestines).

The Cellometer Auto 2000 saves so much time! Experimental data is a lot more reliable when using the Cellometer Auto 2000 than trying to count lavage cells from 20 different samples on hemacytometer.

The Cellometer Auto 2000 is easy to program, troubleshoot, and use. It is so fast and user friendly that it speeds up the research process tremendously! – Renata Linardi, UPENN

The Cellometer Auto 2000 has greatly aided the development of our tissue dissociation assays. The unit allows fast quantification of viable white blood cells from a heterogeneous population following spleen, thymus, lymph node, etc. dissolution which can then quickly proceed to downstream flow-based analysis. The time saved by this method has been extremely valuable! – Stephen Grossman, Takeda Pharmaceuticals

We use the Cellometer Auto 2000 daily to count cells and assess viability of cells derived from a variety of sources. For example, we perform a lot of cell line work, and need accurate counts and viability assessments before using the cells in downstream assays. We work with frozen human PBMC cells as well and require accurate counts and, even more importantly, detailed viability information on the cells that we have thawed, cultured or manipulated in some way. Thus, we use the AOPI reagent to get very accurate counts and viability measurements on our cultured/manipulated/stimulated cells. I’ve personally compared the results using our Cellometer vs. other counting instruments, and have concluded that the Cellometer is superior to all other devices tested! – David DiLillo, Regeneron Pharmaceuticals

The Cellometer Auto 2000 has streamlined our Biorepository Core PBMC counting procedures allowing for a quicker, more precise and more reliable/reproducible cell count. The Auto 2000 has become a cornerstone to our daily Biorepository/ Bio-banking process! – Joseph Keir, Oklahoma medical Research Foundation

My colleague and I have been using the Cellometer Auto 2000 for a year now and absolutely love it! We are doing in vitro stimulation culturing of PBMCs followed by both intracellular cytokine staining and Elispot. The cell counter has facilitated and streamlined our work. Upon receiving the PBMCs, we generally count and then begin culturing the cells under differing growth conditions. After a 14-day culture, we harvest the cels and then set up our assays. The Auto 2000 makes the counting step so much easier! While the differing cultures are in the final wash/spin, we count the cells. This generally takes about 90 seconds for each growth condition, so that by the time the last spin is done, we already know the cell count and have determined what volume they need to be re-suspended in to achieve a linear cell concentration within our assays. No more looking in the microscope and trying to determine whether a cells is dead or not, whether it is a lymphocyte or erythocyte, etc. And the results are consistent! – Chris Elly, Human Longevity, Inc

The Cellometer Auto 2000 is very accurate, easy to use, and there is no need to repeat your count! – Hui Xu, HD Biosciences

The Cellometer Auto 2000 has greatly increased our productivity for in vitro experiments. The automated counting saves hours of time each week compared to manual counting with a hemacytometer! Also being able to quickly access viability of cells is great!

I like using the Cellometer Auto 2000 when counting T cells stained with AOPI. – Bonnie Le, TCR2

The Cellometer Auto 2000 works well, and it has helped speed up the cell counting process compared to manually counting.

The Cellometer Auto 2000 has made functional testing of T cells a much quicker and simpler process than before! When the cell concentration of each population in a 96 well plate must be determined, it is hard to imagine having to count the cells by hand and doing this in a timely manner. Great product!

The Cellometer Auto 2000 is a must-have piece of equipment in the lab! It makes life easy and saves a lot of time counting cells. It’s easy to use and very portable piece of equipment which does not require a lot of space in the lab. If and when a technical issue arises, the tech team will eagerly support you. The team does not hesitate to visit your lab right away if they are in the vicinity of your lab. If not, they will immediately make arrangements to visit you to resolve your issue at the earliest. This is one company, to my experience, that stands by you and their product at all times. – Sanat Dave, UT MD Anderson Cancer Center

I like working with the Cellometer Auto 2000 because I can get quick, consistent results! Before the Auto 2000, our lab was manually counting cells with a hemacytometer. Manual counting can be time-consuming and inconsistent. As a result of this time-saving equipment, we can focus more of our time and energy on what matters most – our research!

I like the Cellometer Auto 2000 in general, I like the accuracy and the ability to use it for different cell types!

On the Cellometer Auto 2000, the AOPI stain is great for routine cell counting, and much more accurate than Trypan Blue. I am grateful the touchscreen is not capacitive, as we wear gloves when using it. – Luis Rodriguez, Regeneron Pharmaceuticals

I use the AOPI viability dye with the Cellometer Auto 2000 to count primary murine and human cells, as well as cell lines. The cell counts are more accurate than traditional Typan Blue method.

We are addicted to using the Cellometer Auto 2000 over grid counting cells. The instant calculation of dilutions, cell diameter, cell viability and a count in each population has helped us tremendously.

The ability to use AOPI dyes in cell counting has greatly increased our ability to count potentially difficult cell populations, such as PBMCs. When the separation has a higher amount of debris present, I feel that I am able to get a more accurate count using this machine, rather than just with Trypan Blue stains!

No more second guessing when counting cells as with the hemacytometer. The Cellometer Auto 2000 is fast, easy, and most of all – accurate! The computer even counts the volume you need for future experiments. The images can be saved as a reference too. The Cellometer makes the whole process very enjoyable! – Carole Grenier, Duke University Medical Center

I did my Postdoctoral work in the Kipins Lab at UVA. As part of a well-funded group looking to do cutting-edge science, I was involved in a lot of the purchasing decisions. We had a gorgeous confocal, a 2-Photon microscope, great FACS capabilities, you name it — but I swear the best thing I recommended was our Nexcelom cell counter! The Cellometer Auto 2000 was easily the most-used piece of equipment in the lab. Super-reliable, accurate, and fantastic support on the rare occasion we needed something adjusted. Now that I’ve moved on to the next phase of my career, I did not even want to see machines from anyone else. HIGHLY recommended! – Noel Derecki, Janssen

The Cellometer Auto 2000 has been a huge help to our lab! Before purchasing, we used to count on the old fashioned glass hemacytometer, always with some variability. Now, all that we do is mix our cells with the AOPI, put them on the slide, and count. It has been great help!

With the Cellometer Auto 2000, our cell viability assay take about 25% of the time it used to take using flow cytometry!

The lab I am in uses the Cellometer Auto 2000. It is so much faster than manually counting cells, and the count is every bit as accurate. It is extrememly easy to use and saves everyone in the lab a lot of time!

We use our Cellometer Auto 2000 daily for our cell culture counting and viability assays. It’s very easy to use, with user-friendly software, quick and accurate cell counting, proper record keeping, and cheap maintenance. It’s a small treasure for our GMP lab and I highly recommend it!

We just recently purchased the Cellometer Auto 2000 system and routinely use it for mesenchymal stem cell count and viability. I like that it’s fully automated, and all the analysis takes less than a minute! It’s more accurate than to manually count with a hemacytometer.

The speed and simplicity of the Cellometer Auto 2000 is great. Such a time-saver over the old-fashioned hemacytometer. Easier and less messy to use than the Beckman Vi-Cell.

The Cellometer Auto 2000 is a great cytometer! It works well counting PBMCs isolated from human blood. We tested the counts in comparison to our old cytometer and by counts made by eye, and the numbers were accurate and reliable!

Our Cellometer Auto 2000 has been a staple in multiple of our projects. We use it on a daily basis and have been very happy with its performance. It is reliable and a huge time saver!

I have used different models of the Nexcelom Cellometers in various labs and it is definitely the standard for cell counting. Results are consistent time and again, the reliability of which is great when performing duplicate or triplicate counts. One of the best things about the Cellometer Auto 2000 in particular is the ability to distinguish dead cells from live ones based on different color staining. It allows for visual confirmation of what the machine is already telling us, which is great when it comes to really low cell counts that can be quite demoralizing. I also enjoy the ability to establish several different settings based on the type of cell, and also appreciate Nexcelom’s customer service! -Melissa Yao, Cold Spring Harbor Laboratory

The Cellometer Auto 2000 is very reliable in operation. It responds quickly between functions and usages without rebooting. It has great consistency between different functions/modes in cell counting.

Thanks to the Cellometer Auto 2000, I can do my flow cytometry and HSC transplantation faster and better, while giving a very repeatable reading!

I absolutely trust the Cellometer Auto 2000 cell count results for primary and immune cells. It’s easy to use and provides the accuracy I need for my experiments. It is one of the best lab purchases! All I know is that I’m never counting primary cells manually again! The customer service is great as well! -Joeseph Castillo, Genentech

Our Cellometer Auto 2000 has greatly improved the speed and accuracy of our cell counting and has allowed us to analyze our cell samples in a variety of ways. The AOPI feature has been integral in generating cell viable data for a few of our new projects. The customer service team is also amazing! They are very knowledgeable about their products and technology and are always available to help us troubleshoot.

The Cellometer Auto 2000 is a very efficeint way to quickly count cells. We have been using it for every cell culture experiment and pass, making that step of the process pretty seamless.

The Cellometer Auto 2000 system has greatly simplified the way my company does cell counting and has become a very essential piece of equipment. We use it for both high and low RBC cell counting for viability purposes to accurately culture large volumes of cells. It has cut down on the amount of time we take to do cell counts and allows us to complete our processes in a very timely fashion. Truly a great product! – Derek Grice, Cognate BioServices, Inc.

We purchased a Cellometer Auto 2000 cell viability counter. It is great for cell counting and viability recordings. The touch screen feature and cell imaging features are great.

My colleague and I have purchased a Cellometer Auto 2000 cell counter back in January of this year. We are using it now and it has facilitated our work greatly. We routinely process PBMCs from both fresh whole blood and from frozen stock. The Cellometer has made it much easier to get cell numbers and viability percentages for use in downstream applications such as IVS and Elispot. -Christian Elly, Human Longevity, Inc

I would cry if I had to work without the Cellometer Auto 2000-Victoria Groysberg, UCLA

Counting cells daily on the microscope was very bad for my occipital joints and muscles. Since using the Cellometer Auto 2000, I no longer have neck pain when counting cells. Thank you Nexcelom! -Tiffany Sidwell, UCLA Immunogenetics Center

Our lab bought a Cellometer Auto 2000. We regularly grow primary human T cells, and with a lot of samples per experiment (20-30+), it has been a lot of work to keep track of growth/expansion and viability prior to having the Cellometer. With the Auto 2000, it is much easier, and much more reliable to count each set of samples and get reliable data on expansion and viability, and we are able to take samples for counts every other day, rather than once a week.

I have used the Cellometer Auto 2000 in my previous lab extensively for counting mouse primary cells. I cannot be thankful enough to you guys for making such a great product. I liked it so much that I convinced my boss at my new lab to buy the Cellometer Auto 2000 in spite of already having a cell counter from different company.

I like the Cellometer Auto 2000 because it eliminates manual counting and our counts are consistent between users. We count cells from primary samples and I like that the RBCs are not counted when we use the AO/PI stain. – Jeff Painter, Moffitt Cancer Center

Using the Cellometer Auto 2000 has been a walk in the park! The instrument is flawless and the digital display is exquisite! This machine has saved me so much time and has allowed me to complete all my work in a timely fashion. Thanks guys! Keep up the good work! – Dylan Johnson, Burleson Research Technologies

The Cellometer Auto 2000 has made it possible to work faster, more efficiently and with more precision. – Maria Agarwal, Johns Hopkins University

I like my Cellometer Auto 2000 because it saves my images that I may go back and review. I also like the histogram and the ability to export to Excel.

We have the Cellometer Auto 2000. This instrument has streamlined our experiments. We have several animal studies that require various cell counts. Given that these studies take place over several months, the Cellometer has given us a mode by which to keep out counts consistent (as opposed to manual counting, which would require the same person to count over the course of experiments and is relatively subjective anyhow). We love how quickly we can obtain our data and feel confident that the instrument is giving us accurate results. It’s also convenient that the Cellometer works with a variety of dyes (AO/PI, Trypan Blue, etc.).

We purchased a Cellometer Auto 2000 in late May and have been very pleased with the way it has already changed the work-flow for almost all of our cell-based projects. We are finding it much easier to generate quantitative data that we trust at a rate of progress well beyond what was possible in our lab before by conventional imaging and image analysis approaches. – Rob Bellin, Holy Cross College