Cellometer Auto 2000 Cell Viability Counter

Touchscreen fluorescent automated cell counter for primary samples

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Saved us a lot of valuable time

The Auto 2000 has improved our counting accuracy and consistency but more importantly has saved us a...

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Jan 17, 2019

Saved us a lot of valuable time

The Auto 2000 has improved our counting accuracy and consistency but more importantly has saved us a lot of valuable time that we now can dedicate to other work. We love the Auto 2000!
Jan 17, 2019

You can trust your results

I enjoy the ease of use of the Auto 2000 - everyone in the lab uses it and we have consistent result...

more
Jan 9, 2019

You can trust your results

I enjoy the ease of use of the Auto 2000 - everyone in the lab uses it and we have consistent results when counting samples. It is also helpful to see which cells are counted as dead and alive, so you can trust your results.
Jan 9, 2019

Visualize

The AO/PI live/dead dye used with the Cellometer Auto 2000 is a great way to see and measure the pro...

more
Jan 9, 2019

Visualize

The AO/PI live/dead dye used with the Cellometer Auto 2000 is a great way to see and measure the proportion of live and dead cells in suspension. You can visualize if the dead cells are dispersed evenly or if clumping is a factor in cell viability.
Jan 9, 2019

Very convenient

This instrument [Cellometer Auto 2000] is very convenient especially for counting the cell number of...

more
Jan 5, 2019

Very convenient

This instrument [Cellometer Auto 2000] is very convenient especially for counting the cell number of multiple samples. However, I find that it is a bit difficult to uncover the cover membrane of the slide.
Jan 5, 2019

Very satisfied

Using the Auto 2000 has saved us a lot of time that would have been wasted on using traditional coun...

more
Dec 20, 2018

Very satisfied

Using the Auto 2000 has saved us a lot of time that would have been wasted on using traditional counting methods. The counter has proven to be very reliable when doing counts in duplicates. Overall, we are very satisfied with this equipment.
Dec 20, 2018

A vast improvement

We are in the process of validating the instrument [Cellometer Auto 2000] prior to going live. Howev...

more
Cord For Life, Inc.
Dec 13, 2018

A vast improvement

We are in the process of validating the instrument [Cellometer Auto 2000] prior to going live. However, the instrument is very easy to use and is a vast improvement over our previous manual counting method.
Cord For Life, Inc.
Dec 13, 2018

Very important in our daily tasks

We use the Cellometer [Auto 2000] every day here in our lab. It is very important in our daily tasks...

more
Dec 11, 2018

Very important in our daily tasks

We use the Cellometer [Auto 2000] every day here in our lab. It is very important in our daily tasks, as well as contributing to our research studies. It is very straightforward to use.
Dec 11, 2018

Far superior to any other method

We love our Auto 2000 cell counter. It is so much more accurate than the trypan blue staining we use...

more
Dec 10, 2018

Far superior to any other method

We love our Auto 2000 cell counter. It is so much more accurate than the trypan blue staining we used to do to count our cells. We count everything from CHO-k1, PMBCs, and mast cells using this machine. We particularly find this method of counting cells is far superior to any other method we have tried when counting cells from a tissue digest. Would highly recommend this machine.
Dec 10, 2018

Quick, reliable, easy to use

We find the Auto 2000 Cellometer to be a quick, reliable, easy to use, and reasonably affordable opt...

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Dec 5, 2018

Quick, reliable, easy to use

We find the Auto 2000 Cellometer to be a quick, reliable, easy to use, and reasonably affordable option for the precision being offered. This instrument is a great addition to any lab!
Dec 5, 2018

Saves tons of time on a daily basis

We really enjoy the convenience of our Nexcelom Cellometer [Auto 2000]. It saves tons of time on a d...

more
Nov 27, 2018

Saves tons of time on a daily basis

We really enjoy the convenience of our Nexcelom Cellometer [Auto 2000]. It saves tons of time on a daily basis. We mainly use it to count live or dead cells in a population and to maintain viable levels of NK92mi cells in the desired concentration range.
Nov 27, 2018

An awesome cell counter

I use the Auto 2000. It is an awesome cell counter. Very easy to use and fast. My cell counts are mo...

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Nov 12, 2018

An awesome cell counter

I use the Auto 2000. It is an awesome cell counter. Very easy to use and fast. My cell counts are more accurate with the Cellometer compared to other cell counters. I also found the webinars and training/support very accessible and helpful.
Nov 12, 2018

Very accurate, reliable, and easy to use

The Cellometer Auto 2000 has completely replaced the Vi-Cell and Countess in my workflow. I primaril...

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Nov 8, 2018

Very accurate, reliable, and easy to use

The Cellometer Auto 2000 has completely replaced the Vi-Cell and Countess in my workflow. I primarily use it for counting and checking the PBMCviability of primary cells and PBMCs using the AO/PI function. I have found it to be very accurate, reliable, and easy to use.
Nov 8, 2018

It plays a crucial role

It [the Cellometer Auto 2000] is very useful and accurate. I process samples every day and it plays ...

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Oct 18, 2018

It plays a crucial role

It [the Cellometer Auto 2000] is very useful and accurate. I process samples every day and it plays a crucial role. It is very easy to use and understand. I came in knowing nothing, but mastering how to use the equipment was quite easy.
Oct 18, 2018

Comparable to hemacytometer

I like the cell images with AO/PI dye. When a cell line didn't have GFP protein just looking at the ...

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Elstar Therapeutics
Oct 11, 2018

Comparable to hemacytometer

I like the cell images with AO/PI dye. When a cell line didn't have GFP protein just looking at the images I could tell that there was something wrong with it. I have compared it [Cellometer Auto 2000] with a hemacytometer and they are comparable while Vi-Cell was not.
Elstar Therapeutics
Oct 11, 2018

Faster, easier, and more effective

We have been counting PBMCs on a coulter counter, and once that broke, a manual hemocytometer. The A...

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Rutgers New Jersey Medical School
Sep 18, 2018

Faster, easier, and more effective

We have been counting PBMCs on a coulter counter, and once that broke, a manual hemocytometer. The Auto 2000 is faster, easier, and more effective than either method, to say nothing of the reduced hassle of cleaning a hemocytometer or constantly flushing and priming an aperture.
Rutgers New Jersey Medical School
Sep 18, 2018

Big part of our research projects

We have been using the Nexcelom [Cellometer] Auto 2000 in our lab and we greatly enjoy using it! We ...

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Sep 9, 2018

Big part of our research projects

We have been using the Nexcelom [Cellometer] Auto 2000 in our lab and we greatly enjoy using it! We have not encountered any problems with it. The instrument is easy to use and is a big part of our research projects.
Sep 9, 2018

Extremely user-friendly

The Cellometer [Auto 2000] is extremely user-friendly, even to those who have never used one. Cell c...

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Sep 6, 2018

Extremely user-friendly

The Cellometer [Auto 2000] is extremely user-friendly, even to those who have never used one. Cell counts are consistent and images are clear.
Sep 6, 2018

Very amenable to customization

We have been very happy with the performance of the Cellometer Auto 2000 as well as the support we g...

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TeVido BioDevices
Aug 29, 2018

Very amenable to customization

We have been very happy with the performance of the Cellometer Auto 2000 as well as the support we got when evaluating the instrument and setting it up. Since it is easy to view what is being counted and adjust the settings, we think it is very amenable to customization and this was important for our application. The instrument is still simple to use despite the ability to adapt all the settings. We haven't been using it very long, but haven't had any issues with it thus far.
TeVido BioDevices
Aug 29, 2018

See my cell suspension prior to counting

I really love that I can use the Cellometer Auto 2000 to see my cell suspension prior to counting. I...

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Aug 1, 2018

See my cell suspension prior to counting

I really love that I can use the Cellometer Auto 2000 to see my cell suspension prior to counting. If my cells look too dense and clumpy, I can further dilute my cell suspension and test another sample to see that they are suspended in sufficient volume to get accurate counts.
Aug 1, 2018

Everyone in the lab prefers the Cellometer

We have multiple counting instruments (flow cytometer, trypan blue, Scepter, Nucleocounter) but ever...

more
Aug 1, 2018

Everyone in the lab prefers the Cellometer

We have multiple counting instruments (flow cytometer, trypan blue, Scepter, Nucleocounter) but everyone in the lab prefers the Cellometer [Auto 2000] due to its reliability and ease of use.
Aug 1, 2018

Introduction to the Cellometer Auto 2000 Cell Viability Counter

Simple, User-friendly Procedure

Simple, User-friendly Procedure for Cellometer Auto 2000

  1. Pipette 20 µl
  2. Insert Slide
  3. Select Assay & Click Count
  4. View Results in 30 seconds!

Simple, Automated Cell Counting in 30 Seconds

The Auto 2000 utilizes bright field imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

Results in less than 30 seconds

Load Sample, View Image, Count Cells, and Obtain Results in less than 30 seconds

The Auto 2000 Allows Users to:

  • Increase throughput
  • Increase accuracy
  • Improve consistency
  • Ensure all data is correctly captured
  • Count difficult cells (clumpy, irregular-shaped)
  • Eliminate judgment errors, miscounts, interference from red blood cells and user-to-user variability

Primary Cell Analysis: PBMCs, Stem Cells, and more

The Cellometer Auto 2000 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including:

  • Nucleated Cells for Transplantation
  • PBMCs for Immunology
  • Splenocytes for Vaccine Development
  • Stem Cells for Cellular Therapy
  • Tumor Cell Suspensions for Oncology

Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both ‘messy’ and ‘clean’ samples enables the Auto 2000 to evaluate samples at a variety of points throughout sample processing – from initial collection to separation, to cryopreservation.

The Cellometer Auto 2000 features one-touch assays for analysis of a wide range of primary samples, including:

Nucleated Cells from Whole Blood

Nucleated Cells from Whole Blood using Cellometer Auto 2000

Stem Cells

Stem Cells, image from Cellometer Auto 2000

PBMCs / Splenocytes following Separation

PBMCs / Splenocytes following Separation using Cellometer Auto 2000

Tumor and Tissue Cell Suspension

Tumor and Tissue Cell Suspension using Cellometer Auto 2000

No Interference from Red Blood Cells, Platelets, or Debris

The dual-fluorescence AO/PI method utilizes nuclear staining dyes that bind to nucleic acids in the cell nucleus. Because most mature mammalian red blood cells do not contain nuclei, only live and dead mononuclear cells produce a fluorescent signal. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step. Red blood cells, platelets, and debris are not counted in the fluorescent channels.

The advantage of fluorescent counting for primary cells

These images (right) demonstrate the advantage of fluorescent counting for primary cells. The bright field image shows the combination of nucleated cells, red blood cells, and platelets present in the sample. Only the live and dead nucleated cells are visualized and counted in the green and red fluorescent channels.

Sample Measurement Total nucleated All RBC % RBC n
PBMC+RBC Mean

CV

1.26E+07

6.2%

1.39E+07

8.8%

1.23E+06 8.9% 10
PBMC+1/2RBC Mean

CV

1.21E+07

4.8%

1.27E+07

5.4%

5.82E+05 4.6% 10
PBMC+1/4RBC Mean

CV

1.22E+07

7.9%

1.24E+07

7.3%

2.27E+05 1.8% 10

Fresh human PBMCs (peripheral blood mononuclear cells) were spiked with varying amounts of RBCs (red blood cells.) All cells (nucleated + RBC) were counted in the bright field channel. Nucleated cells were then counted in the green fluorescent channel. Varying amounts of RBCs (1.8%, 4.6%, and 8.9%) did not affect the nucleated cell count.

Several red blood cells are indicated in the bright field image (above, left). The red blood cells are not visible in the fluorescent image (above, right) detecting cells stained with nuclear staining dye.

Analysis of Clumpy and Irregular-shaped Cells

Including NCI-60 and clumpy MCF-7 Cells

NCI-60 is a group of 59 human cancer cell lines (originally 60) developed by the National Cancer Institute for screening purposes.

  • 57% of the NCI-60 cell lines are clumpy, contain debris, or display large variations in cell shape or size
  • All 59 NCI-60 cell lines have been successfully validated on the Cellometer Cell Counter

All 40 of the NCI Comprehensive Cancer Centers use Cellometer Cell Counters.

Clumpy Cells

The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown above).

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells and activated T-cells.

User-Friendly Touch Screen

The user-friendly touch screen displays pre-optimized assays for common cell types. User-specific protocols can be easily created and saved to the menu. The simple, pre-optimized assays make the instrument easy to operate and simplify training for new users.

With the click of a button, researchers can select a new assay with saved counting parameters, making the instrument ideal for labs testing a variety of cell types. Auto-save options allow users to quickly test a series of samples.

Live / Dead Nucleated Cell Counts using Dual-Fluorescence

Green fluorescent live cell image

Red fluorescent dead cell image

Why Dual-Fluorescence?

Because bright field cell counting does not differentiate nucleated from non-nucleated cells and trypan blue staining is not as easy to detect as fluorescent staining, dual-color fluorescence is strongly recommended for accurate viability analysis for primary cells. The Auto 2000 is equipped with standard assays for dual-fluorescence analysis of primary cells stained with AO/PI.

The AO/PI Method

Acridine Orange, AO, is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence. Propidium iodide, PI, can only enter dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.

Cell Images for Data Verification

No two cells are the same.

With the Auto 2000 Cell Counter, cell morphology can be immediately viewed on-screen in the bright field image.

Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly. Bright field counted images can be viewed for basic cell counting and trypan blue viability.

Fluorescent counted images indicating counted live and dead nucleated cells can be viewed for dual-fluorescence primary cell viability assays.

Users can confirm that:

  • cells are counted correctly, based on size and shape
  • cells within clumps are being counted individually
  • red blood cells, platelets, and debris are being excluded from results

The bright field image confirms that individual cells within pairs are being counted and smaller debris is not being counted. In the combined fluorescent counted image, live counted cells are circled in green. Dead counted cells are circled in red.

  • Cell images can be archived and exported for use in publications and presentations.
  • Saved images can be re-counted using default or user-optimized analysis settings

Cell Size Analysis & Size-based Counting

The Auto 2000 Cell Counter Automatically generates a cell size histogram based on cell diameter.

Because Cellometer generates individual cell size measurements, multiple samples can be overlaid on one histogram enabling analysis of the change in cell diameter over time

For experiments involving SF9 or SF21 cells, saved images can be viewed and re-counted to analyze changes in morphology over time.

The minimum and maximum cell diameter settings can be optimized to count specific cells in a sample.

This example demonstrates counting of mature dendritic cells cultured from PBMCs based on cell diameter.

10x Faster than Manual Counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto 2000 Cell Viability Counter calculates cell count and concentration for live and dead cells and % viability in just 30 seconds.

Improve Data Accuracy & Consistency

  • Eliminate Wash Steps
  • Eliminate Judgment Errors
  • Eliminate interference from RBCs
  • Eliminate Recording & Calculation Errors
  • Reduce Counting Time … Run More Experiments

Cellometer Precision

The Cellometer Auto 2000 offers excellent reproducibility, with a %CV (Coefficient of Variation) of <10% for fluorescent concentration and viability measurements. The data (below) is based on four preparations of Jurkat cells stained with propidium iodide, a fluorescent nuclear-staining dye.

Sample N Value Average Live Cell Concentration % Viability CV of Concentration CV of Viability
A 4 4.20E+06 91.1 10% 2%
B 4 1.06E+06 22.7 7% 1%
C 4 3.27E+06 57.5 7% 7%

Imaging / Counting Chambers: No Washing or Contamination

Cellometer Disposable Imaging Chambers consist of two independent enclosed chambers with a precisely controlled height. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells.

The disposable Cellometer Cell Counting Chambers offer several key advantages:

Time savings – no washing

No risk of cross-contamination

Reduced biohazard risk to users

Controlled sample volume

Large-depth chambers for large cells

Most affordable automated counting consumables

Auto-Save Data and Cell Images

The Auto 2000 allows users to generate specific folders to which data reports and images can be auto-saved. Using the auto-save feature ensures all data is accurately captured in the correct location. It also enables users to test a series of samples very quickly

Users can configure the Auto 2000 to request a new sample name for each sample that is imaged and counted or apply auto-numbering to individual samples in a series to further reduce analysis time. Users can also apply a time stamp to individual data files.

Dedicated On-line and On-site Applications Support

Experienced Nexcelom Technical Support Specialists are available from 8:30am to 5:00pm EST for phone and on-line support and can assist with:

  • Creation of new cell types
  • Optimization of counting parameters
  • Troubleshooting
  • Training of new users
  • Installation of the Auto 2000 Cell Viability Counter

The help button at the bottom right of the Cellometer Auto 2000 software screen gives users instant access to:

  • Software features and instructions
  • On-line tutorials and training videos
  • Submission of a Support Ticket

All Nexcelom Applications Specialists are 100% focused on image-based cell concentration & viability and cell-based assays using Cellometer Image Cytometry.

Nexcelom Field-based Applications Specialists are also available for:

  • On-site demonstrations
  • Training
  • Troubleshooting
  • Technical Seminars

Applications for Cellometer Auto 2000 Cell Viability Counter

Adipocytes

Adipocytes

Automatically measure cell size of freshly isolated adipocytes and plot size histogram. DNA staining fluorescence dyes are used to identify cells from lipid droplets. »

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Use Cellometer to perform cell based assays and measure cell size, viability and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Viability and Necrosis

Cell Viability and Necrosis

Cell viability is performed using various fluorescent membrane exclusion dyes, such as PI, EB, 7AAD, and others. This assay is performed by enumerating cells in captured bright-field and fluorescent images. And Necrotic cells are detected using propidium iodide. »

Blood Samples

TNC Concentration & Viability for Clinical (Blood) Samples

Analyze fresh and processed blood and bone marrow samples without lysing: no interference from RBCs. »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Automatically measure live cell concentration and viability of cancer cell lines used in oncology research and most of all biology research. »

GFP Transfection Efficiency

Quantitative Measurement of GFP Transfection

Rapidly identify fluorescence positive cells from a sample, analyze individual cell fluorescence intensity, calculate cell concentration, size and determine the GFP transfection automatically. »

Immunology

Immunology Research

Automatically quantify cell viability and concentration for a variety of immunologically relevant samples such as: bone marrow, cord blood, slpenocytes, lymphocytes, isolated mononuclear cells, tumor digests, murine samples, and others. »

Insect Cells

Insect Cells

Automatically measure live cell concentration, viability for baculovirus infected insect cells. Cell size histogram live cell concentration and viability are generated within less than 60 seconds using 20 µl sample. »

PBMC

Peripheral Blood Mononuclear Cells (PBMC)

Automatically measure live cell concentration and viability without lysing red blood cells for consistent results from patient samples. Other cells include splenocytes and bone marrow. »

Platelets

Platelets

Automatically measure mouse and human platelet concentration using brightfield only method. »

Stem Cells

Stem Cells

Automatically measure live cell concentration and viability for stem cells, such as human and mouse ES cells, mesenchymal, cardiac, induced pluripotent stem cells. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells. For samples with a lot of debris, dual fluorescent nuclear stains are used for live and dead cells. »

WBSc in Whole Blood

WBCs in Whole Blood

Automatically measure nucleated cell concentration without lysing red blood cells using nuclear staining dyes (AO), for human and mouse blood. »

Stem Cells, Primary Cells, Cell Line

The results indicate the accuracy of the Cellometer Auto 2000 instrument in assessing the viability of Jurkat cells using AO/PI for cell viability. Four measurements were performed for each sample. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring cell concentration and viability of mammalian cells.

Stem Cells, Primary Cells, Cell LineFigure 2: Chart of the % viable cells at 3 different live/dead mixture ratios. N=4 per measurement. The error bars correspond to the standard deviation for each measurement.
Sample N Value Average Live Cell Concentration % Viability CV of Concentration CV of Viability
A 4 4.10E+06 78 1% 2%
B 4 0.0025E+06 0.6 N/A N/A
C 4 2.48E+06 36 4% 7%
Figure 1: Table of results for AO/PI viability using the Cellometer Auto 2000.

Cell Line Viability using Propidium Iodide Only

The results indicate the accuracy of the Cellometer Auto 2000 instrument in assessing the viability of Jurkat cells using PI for cell viability. Four measurements were performed for each sample. The viability average was calculated and plotted. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring cell concentration and viability of mammalian cells.

Cell Line Viability using Propidium Iodide Only
Figure 2: Chart of the % viable cells at 3 different live/dead mixture ratios. N=4 per measurement. The error bars correspond to the standard deviation for each measurement.
Sample N Value Average Live Cell Concentration % Viability CV of Concentration CV of Viability
A 4 4.20E+06 91.1 10% 2%
B 4 1.06E+06 22.7 7% 1%
C 4 3.27E+06 57.5 7% 7%
Figure 1: Table of results for cell viability using PI only.

Cell Line Total Cell Concentration

The results indicate the accuracy of the Cellometer Auto 2000 instrument cell concentration. Serial dilutions (2-, 4-, and 8-fold) were prepared using PBS and Jurkat cells. Four measurements were performed for each sample and the averages were calculated and plotted. A linear correlation between the concentration measurements and 1/dilution factor was observed. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring cell concentration.

Cell Line Total Cell Concenration
Figure 2: Jurkat cell concentrations at various sample dilution factors. N=4. Error bars correspond to the Standard Deviations for cell concentration.
Sample Dilution Factor Average Cell Concentration CV of Concentration Standard Deviation N Value
A 1 5.80E+06 1% 4.8E+04 4
B 2 3.39E+06 2% 8.12E+04 4
C 4 1.63E+06 6% 9.54E+04 4
D 8 8.27E+05 6% 5.33E+04 4
Figure 1: Table of results for total cell concentration for the Cellometer Auto 2000 instrument.

Low Concentration of Cells using Acridine Orange

The results indicate the accuracy of the Cellometer Auto 2000 instrument when counting Jurkat cells at very low concentrations (between 5-7E+04 cells/ml). AO was used to measure the concentration of cells in the F1 channel. Results from 4 measurements were plotted. The CV values are very close, which indicates the consistency of the measurements. The results show the reliability and accuracy of the Cellometer Auto 2000 in measuring at low cell concentration.

Low Concentration of Cells using Acridine Orange
Figure 2: Graph of Concentration for 4 different measurements using the Low Concentration of Cells Assay and AO on the Cellometer Auto 2000.
Low Concentration AO F1 Concentration
Sample 1 5.91E+04
Sample 2 6.40E+04
Sample 3 6.58E+04
Sample 4 6.90E+04
Average 6.45E+04
STDEV 4.14E+03
CV 6%
Figure 1: Table of results from the Low Concentration of Cells assay.

Performance of the Cellometer Auto 2000 Cell Viability Counter

Consistency and Accuracy Comparison to Hemacytometer

Jurkat

N = 20 Hemacytometer Cellometer Auto 2000
Average 1.03E+06 1.01E+06
STDEV 6.60E+04 8.87E+04
%CV 6.4% 8.8%

5 µm beads

N = 20 Hemacytometer Cellometer Auto 2000
Average 1.07E+06 1.03E+06
STDEV 5.90E+04 4.30E+04
%CV 5.5% 4.2%

Specifications for Cellometer Auto 2000 Cell Viability Counter

Cellometer Auto 2000
Includes • Cellometer Auto 2000 Instrument with integrated computer and
touch-screen and dual-fluorescent optics
• Cellometer Software
• Two USB 2.0 ports
• Power Supply
• Ethernet port
• Phone/online applications support during set-up
Imaging Performance Cell Size: 5 – 300* microns
Conc. Range: 105 – 107 cells/mlBright field imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
*Cellometer CHT4-PD300 Slides are required for cells > 80 microns in diameter
Instrument Specifications Weight: 26 lbs. (11.8 kg)
Dimensions: Width: 11.1″ (28.3 cm)
Depth: 12.8″ (32.4 cm)
Height: 13.4″ (34.0 cm)Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.5A
Output to Instrument: 12 VDC, 7.5A
Display 10.4″ touch screen
Fluorescent Optics Excitation / Emission: 470 nm/535 nm
Example Fluorophores:
• Acridine Orange (+DNA)
• Calcein AM
• CFDA
• SYTO® 9
• SYTO® 13Excitation / Emission: 540 nm/605 nm
Example Fluorophores:
• Propidium Iodide
• Ethidium Bromide

Customer Testimonials

Saved us a lot of valuable time

The Auto 2000 has improved our counting accuracy and consistency but more importantly has saved us a...

more
Jan 17, 2019

Saved us a lot of valuable time

The Auto 2000 has improved our counting accuracy and consistency but more importantly has saved us a lot of valuable time that we now can dedicate to other work. We love the Auto 2000!
Jan 17, 2019

You can trust your results

I enjoy the ease of use of the Auto 2000 - everyone in the lab uses it and we have consistent result...

more
Jan 9, 2019

You can trust your results

I enjoy the ease of use of the Auto 2000 - everyone in the lab uses it and we have consistent results when counting samples. It is also helpful to see which cells are counted as dead and alive, so you can trust your results.
Jan 9, 2019

Visualize

The AO/PI live/dead dye used with the Cellometer Auto 2000 is a great way to see and measure the pro...

more
Jan 9, 2019

Visualize

The AO/PI live/dead dye used with the Cellometer Auto 2000 is a great way to see and measure the proportion of live and dead cells in suspension. You can visualize if the dead cells are dispersed evenly or if clumping is a factor in cell viability.
Jan 9, 2019

Very convenient

This instrument [Cellometer Auto 2000] is very convenient especially for counting the cell number of...

more
Jan 5, 2019

Very convenient

This instrument [Cellometer Auto 2000] is very convenient especially for counting the cell number of multiple samples. However, I find that it is a bit difficult to uncover the cover membrane of the slide.
Jan 5, 2019

Very satisfied

Using the Auto 2000 has saved us a lot of time that would have been wasted on using traditional coun...

more
Dec 20, 2018

Very satisfied

Using the Auto 2000 has saved us a lot of time that would have been wasted on using traditional counting methods. The counter has proven to be very reliable when doing counts in duplicates. Overall, we are very satisfied with this equipment.
Dec 20, 2018

A vast improvement

We are in the process of validating the instrument [Cellometer Auto 2000] prior to going live. Howev...

more
Cord For Life, Inc.
Dec 13, 2018

A vast improvement

We are in the process of validating the instrument [Cellometer Auto 2000] prior to going live. However, the instrument is very easy to use and is a vast improvement over our previous manual counting method.
Cord For Life, Inc.
Dec 13, 2018

Very important in our daily tasks

We use the Cellometer [Auto 2000] every day here in our lab. It is very important in our daily tasks...

more
Dec 11, 2018

Very important in our daily tasks

We use the Cellometer [Auto 2000] every day here in our lab. It is very important in our daily tasks, as well as contributing to our research studies. It is very straightforward to use.
Dec 11, 2018

Far superior to any other method

We love our Auto 2000 cell counter. It is so much more accurate than the trypan blue staining we use...

more
Dec 10, 2018

Far superior to any other method

We love our Auto 2000 cell counter. It is so much more accurate than the trypan blue staining we used to do to count our cells. We count everything from CHO-k1, PMBCs, and mast cells using this machine. We particularly find this method of counting cells is far superior to any other method we have tried when counting cells from a tissue digest. Would highly recommend this machine.
Dec 10, 2018

Quick, reliable, easy to use

We find the Auto 2000 Cellometer to be a quick, reliable, easy to use, and reasonably affordable opt...

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Dec 5, 2018

Quick, reliable, easy to use

We find the Auto 2000 Cellometer to be a quick, reliable, easy to use, and reasonably affordable option for the precision being offered. This instrument is a great addition to any lab!
Dec 5, 2018

Saves tons of time on a daily basis

We really enjoy the convenience of our Nexcelom Cellometer [Auto 2000]. It saves tons of time on a d...

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Nov 27, 2018

Saves tons of time on a daily basis

We really enjoy the convenience of our Nexcelom Cellometer [Auto 2000]. It saves tons of time on a daily basis. We mainly use it to count live or dead cells in a population and to maintain viable levels of NK92mi cells in the desired concentration range.
Nov 27, 2018

An awesome cell counter

I use the Auto 2000. It is an awesome cell counter. Very easy to use and fast. My cell counts are mo...

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Nov 12, 2018

An awesome cell counter

I use the Auto 2000. It is an awesome cell counter. Very easy to use and fast. My cell counts are more accurate with the Cellometer compared to other cell counters. I also found the webinars and training/support very accessible and helpful.
Nov 12, 2018

Very accurate, reliable, and easy to use

The Cellometer Auto 2000 has completely replaced the Vi-Cell and Countess in my workflow. I primaril...

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Nov 8, 2018

Very accurate, reliable, and easy to use

The Cellometer Auto 2000 has completely replaced the Vi-Cell and Countess in my workflow. I primarily use it for counting and checking the PBMCviability of primary cells and PBMCs using the AO/PI function. I have found it to be very accurate, reliable, and easy to use.
Nov 8, 2018

It plays a crucial role

It [the Cellometer Auto 2000] is very useful and accurate. I process samples every day and it plays ...

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Oct 18, 2018

It plays a crucial role

It [the Cellometer Auto 2000] is very useful and accurate. I process samples every day and it plays a crucial role. It is very easy to use and understand. I came in knowing nothing, but mastering how to use the equipment was quite easy.
Oct 18, 2018

Comparable to hemacytometer

I like the cell images with AO/PI dye. When a cell line didn't have GFP protein just looking at the ...

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Elstar Therapeutics
Oct 11, 2018

Comparable to hemacytometer

I like the cell images with AO/PI dye. When a cell line didn't have GFP protein just looking at the images I could tell that there was something wrong with it. I have compared it [Cellometer Auto 2000] with a hemacytometer and they are comparable while Vi-Cell was not.
Elstar Therapeutics
Oct 11, 2018

Faster, easier, and more effective

We have been counting PBMCs on a coulter counter, and once that broke, a manual hemocytometer. The A...

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Rutgers New Jersey Medical School
Sep 18, 2018

Faster, easier, and more effective

We have been counting PBMCs on a coulter counter, and once that broke, a manual hemocytometer. The Auto 2000 is faster, easier, and more effective than either method, to say nothing of the reduced hassle of cleaning a hemocytometer or constantly flushing and priming an aperture.
Rutgers New Jersey Medical School
Sep 18, 2018

Big part of our research projects

We have been using the Nexcelom [Cellometer] Auto 2000 in our lab and we greatly enjoy using it! We ...

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Sep 9, 2018

Big part of our research projects

We have been using the Nexcelom [Cellometer] Auto 2000 in our lab and we greatly enjoy using it! We have not encountered any problems with it. The instrument is easy to use and is a big part of our research projects.
Sep 9, 2018

Extremely user-friendly

The Cellometer [Auto 2000] is extremely user-friendly, even to those who have never used one. Cell c...

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Sep 6, 2018

Extremely user-friendly

The Cellometer [Auto 2000] is extremely user-friendly, even to those who have never used one. Cell counts are consistent and images are clear.
Sep 6, 2018

Very amenable to customization

We have been very happy with the performance of the Cellometer Auto 2000 as well as the support we g...

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TeVido BioDevices
Aug 29, 2018

Very amenable to customization

We have been very happy with the performance of the Cellometer Auto 2000 as well as the support we got when evaluating the instrument and setting it up. Since it is easy to view what is being counted and adjust the settings, we think it is very amenable to customization and this was important for our application. The instrument is still simple to use despite the ability to adapt all the settings. We haven't been using it very long, but haven't had any issues with it thus far.
TeVido BioDevices
Aug 29, 2018

See my cell suspension prior to counting

I really love that I can use the Cellometer Auto 2000 to see my cell suspension prior to counting. I...

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Aug 1, 2018

See my cell suspension prior to counting

I really love that I can use the Cellometer Auto 2000 to see my cell suspension prior to counting. If my cells look too dense and clumpy, I can further dilute my cell suspension and test another sample to see that they are suspended in sufficient volume to get accurate counts.
Aug 1, 2018

Everyone in the lab prefers the Cellometer

We have multiple counting instruments (flow cytometer, trypan blue, Scepter, Nucleocounter) but ever...

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Aug 1, 2018

Everyone in the lab prefers the Cellometer

We have multiple counting instruments (flow cytometer, trypan blue, Scepter, Nucleocounter) but everyone in the lab prefers the Cellometer [Auto 2000] due to its reliability and ease of use.
Aug 1, 2018

This machine works great!

This machine [Cellometer Auto 2000] works great! So much easier and more accurate than counting manu...

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Jul 31, 2018

This machine works great!

This machine [Cellometer Auto 2000] works great! So much easier and more accurate than counting manually.
Jul 31, 2018

Very quick and efficient

From what I've heard from my mentors, cell counting sounded like a drag: grids, manual counting, yad...

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Jul 24, 2018

Very quick and efficient

From what I've heard from my mentors, cell counting sounded like a drag: grids, manual counting, yadda yadda yadda. But with this device [Cellometer Auto 2000], cell counting is very quick and efficient. The setup work in the tube is quite simple, and counting is as easy as literally pressing a button.
Jul 24, 2018

Extremely useful

The Auto 2000 is extremely useful for our laboratory. We use one a lot of times during our day. Cell...

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Brigham and Women's Department of Pathology
Jul 6, 2018

Extremely useful

The Auto 2000 is extremely useful for our laboratory. We use one a lot of times during our day. Cellometer helps do experiments much faster than before.
Brigham and Women's Department of Pathology
Jul 6, 2018

We are confident in the accuracy

This instrument is very helpful in our lab - we use it for AO/PI, trypan, and bright field staining ...

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Jul 5, 2018

We are confident in the accuracy

This instrument is very helpful in our lab - we use it for AO/PI, trypan, and bright field staining for at least 5 different cell types and we are confident in the accuracy of the [Cellometer] Auto 2000.
Jul 5, 2018

Very supportive staff

Ease of use is probably the most dominating feature [of the Cellometer Auto 2000]. Backed by a very ...

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Jun 22, 2018

Very supportive staff

Ease of use is probably the most dominating feature [of the Cellometer Auto 2000]. Backed by a very supportive staff, using instruments or services through Nexcelom has been a pleasure.
Jun 22, 2018

Ease of use

Ease of use is probably the most dominating feature. Backed by a very supportive staff, using instru...

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Jun 22, 2018

Ease of use

Ease of use is probably the most dominating feature. Backed by a very supportive staff, using instruments or services through Nexcelom has been a pleasure.
Jun 22, 2018

FAQs

Cellometer Auto 2000 has no liquid handling fluidics system. Therefore, cleaning related maintenance is eliminated completely. Optical light sources in Auto2000 are light emitting diodes, with standard lifetime of more than 10,000 hours. Therefore minimum maintenance is required for optics either. Some customers purchase yearly preventative maintenance contract from Nexcelom. We also offer extended warranty. Costs of the services vary.
We will issue a RMA for the unit to be shipped back to Nexcelom for repair. Our standard turn-around time for repair in USA is less than one week after receiving the instrument.
Single board computer running Windows embedded operating system and touch screen.

Imaging capability includes bright field and two fluorescence channels with excitation / emission of 470nm/535 nm and 540nm/635 nm, respectively.

We release software based on customer needs.

No. The most complicated primary hepatocytes are measured using a special software algorithm available only with Cellometer Vision.

Brochure

Videos

Application Notes

Training Webinars

Our customers include: