Cellometer K2 Fluorescent Cell Counter

Advanced Cell Counter with Option for 21 CFR Part 11 Module

Cellometer K2 with 21 CFR Part 11

“This instrument [Cellometer K2] is definitely worth the investment.”

“This machine [Cellometer K2] has transformed the way I culture.”

“… a very good alternative to flow cytometer …”

“This is so convenient and easy to use.”

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Virtual or In-lab Demo

Features of the Cellometer K2 Cell Counter

The Cellometer K2, powered by Matrix software, utilizes brightfield imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

The Cellometer K2 has the following advantages:

  • Dual fluorescence and brightfield imaging – stain only nucleated cells for the
    most accurate count and viability information
  • Fast results – count, size, concentration, and viability calculations in <60 seconds
  • Analyze complex samples – designed for analysis of complex and messy samples
    including whole blood, peripheral blood, cord blood, and bone marrow
  • Multiple fields of view – increased accuracy with the ability to capture one, four, or
    eight images per sample
  • Built-in predefined assays – quickly analyze viability, apoptosis, and transfection efficiency
  • Built-in cell types – includes saved parameters for over 400 cell types
  • Small sample volume – only 10 µl of cell sample required
  • Customizable reports – includes predefined reports with the ability to create new ones
    with graphs, images, charts, and tables
  • Multi-language support – over 7,000 languages available
  • 21 CFR Part 11 ready – optional add-on that includes an audit trail, user access
    control, and digital signature

“The Cellometer K2, coupled with ViaStain AOPI stain, allows users to easily stain nucleated cells in samples containing red blood cells. No lysis buffer is required, which makes getting results very quick.”

Susan Hamilla, Sorrento Therapeutics

Simple and Fast Cell Counting Workflow with Results in Seconds

k2 simple workflow
Matrix software

“We love our Cellometer K2 and every lab should have one! Gone are the days of manual cell counting and we can now reliably and quickly count thousands of cells in a few seconds.”

Nav Masani, AstraZeneca

Accuracy from Cell Lines to Primary Samples

The Cellometer K2 can be customized to handle a variety of cell types, including primary cells, tumor digest, insect cells, cell lines, fragile cells, and more at low or high concentrations.

K2 cell image examples

Predefined Assays and Cell Types

Take the guesswork out of setting up your cell quantification experiments. The Cellometer K2 comes with frequently used assays and cell types with predefined settings to ensure consistent results from sample to sample. Easily build custom assays and cell types to fit your experimental needs.

Not sure what settings to use? Our customer success team and field application specialists are here to help you develop fit-for-purpose assays and protocols for your specific research and development requirements.

“This unit works fast to get accurate and precise numbers. It takes a fraction of the time trypan blue or other manual methods for cell counting. It allows for greater throughput of samples and simplifies our workload. It is simple to use and miles ahead of any of the competitors. ”

Matthew Wilgo, New England Cord Blood Bank
k2 predefined assays

Capture Multiple Fields of View

Multiple Fields of View

Capture one, four, or eight images per sample. The instrument default is set to four images, which is the equivalent of six quadrants on a hemocytometer. Eight images are equivalent to twelve quadrants on a hemocytometer. The ability to capture multiple images improves the cell counting dynamic range and accuracy of results.

“[Cellometer] K2 allows us for faster, reliable and more accurate cell counts that our lab uses so frequently. The process itself is simple and intuitive compared to the other machine we used to have. Such a great improvement!”

Hanh Chi Do, Northwestern University

Dual-fluorescent Staining for Clumpy Cells

The fluorescent image (far right) shows bright green AO-positive hepatocytes declustered by the Cellometer K2 algorithm. Red circled hepatocytes are PI-positive (dead) while free nuclei are not counted.

counting clumpy cells

Predefined and Customizable Reports

Use default or customized reports based on the data and images you want to be included for your specific experimental needs. Automatically export images and data reports including CSV, Excel, Word, or PDF files. Perform statistical analysis for a wide range of parameters such as average, variance, min/max, and standard deviation of cell size.

custom report with histogram
Custom report with scatter plot

21 CFR Part 11 Ready

An optional module can be purchased to meet the requirements for 21 CFR Part 11. The additional module comes with the following features:

  • User login with passwords
  • User assigned permissions
  • Audit trail
  • Error log files
  • Electronic signatures
21 CFR Part 11 ready

Live/Dead Nucleated Cell Counts using Dual-fluorescence

Why Dual-fluorescence?

Because brightfield cell counting does not differentiate nucleated from non-nucleated cells and trypan blue staining is not as easy to detect as fluorescent staining, dual-color fluorescence is strongly recommended for accurate viability analysis for primary cells. The K2 is equipped with standard assays for dual-fluorescence analysis of a variety of cells stained with Acridine Orange and Propidium Iodide (AO/PI).

The AO/PI Method

Acridine Orange (AO) is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence. Propidium Iodide (PI) can only enter dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.

Brightfield vs AO/PI

The brightfield image on the left shows the combination of nucleated cells, red blood cells, and platelets present in the sample. The red blood cells are not visible in the fluorescent image on the right, only the live (green) and dead (red) nucleated cells are counted.

No Interference from Red Blood Cells, Platelets, or Debris

The dual-fluorescence AO/PI method utilizes nuclear staining dyes that bind to nucleic acids in the cell nucleus. Because most mature mammalian red blood cells do not contain nuclei, only live and dead mononuclear cells produce a fluorescent signal. There is no need to lyse red blood cells, saving time and eliminating an extra sample preparation step. Red blood cells, platelets, and debris are not counted in the fluorescent channels.

Analysis of Clumpy and Irregular-shaped Cells

Including NCI-60 and clumpy MCF-7 Cells

NCI-60 is a group of 59 human cancer cell lines (originally 60) developed by the National Cancer Institute for screening purposes.

  • 57% of the NCI-60 cell lines are clumpy, contain debris, or display large variations in cell shape or size
  • All 59 NCI-60 cell lines have been successfully validated on the Cellometer Image Cytometer

All 40 of the NCI Comprehensive Cancer Centers use Cellometer Cell Counters.

Clumpy Cells - Cellometer K2, cell viability counter

Clumpy Cells

The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown above).

Irregular-shaped Cells - Cellometer K2, cell viability counter

Irregular-shaped Cells

The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells and activated T-cells.

10x Faster than Manual Counting

Counting 1 x 106 cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer K2 Image Cytometer calculates cell count and concentration for live and dead cells and % viability in just 60 seconds.

Hemacytometer under microscope using Cellometer K2, cell viability counter

Improve Data Accuracy & Consistency

  • Eliminate wash steps
  • Eliminate judgment errors
  • Eliminate interference from RBCs
  • Eliminate recording & calculation errors
  • Reduce counting time … run more experiments

Imaging / Counting Chambers: No Washing or Contamination

Cellometer disposable images chambers for Cellometer K2, cell viability counter

We offer two different disposable counting slides, one with protective coverings on both sides and a ready-to-use option packed in microscope slide boxes.

Several key advantages include:

  • No clogging
  • Time savings — no washing
  • No risk of cross-contamination
  • Reduce biohazard risk to users

Applications for Cellometer K2 Fluorescent Viability Cell Counter

PBMC

Peripheral Blood Mononuclear Cells (PBMC)

Measure live cell concentration and viability without lysing red blood cells for consistent results from patient samples. »

GFP Transfection Efficiency

Measure GFP Transfection

Rapidly identify fluorescence-positive cells from a sample, calculate cell concentration, size, and determine the GFP transfection percentage automatically. »

WBSc in Whole Blood

WBCs in Whole Blood

Measure nucleated cell concentration without lysing red blood cells using nuclear staining dyes (AO), for human and mouse blood. »

Immunology

Immunology Research

Quantify cell viability and concentration for a variety of immunologically relevant samples such as: bone marrow, cord blood, splenocytes, lymphocytes, isolated mononuclear cells, tumor digests, murine samples, and others. »

Insect Cells

Insect Cells

Measure live cell concentration and viability for baculovirus infected insect cells. »

Hepatocytes

Fresh & Cryo Preserved Primary Hepatocytes

Measure live hepatocyte concentration and viability from fresh and cryo preserved samples using dual-fluorescent nuclear stains for human, rat, mouse, and horse. »

Cancer Cell Lines

NCI-60 Cancer Cell Lines

Measure live cell concentration and viability of cancer cell lines used in oncology research and biology research. »

Adoptive Cell Transfer Therapy

Adoptive Cell Transfer Therapy

Perform cell based assays and measure cell size, viability, and concentration of cell lines and primary samples used in adoptive cell therapy research. »

Apoptosis

Apoptosis and Necrosis

Detect and analyze apoptotic and necrotic cells with Annexin V and PI. »

Trypan Blue and AO/PI

Cell Viability Measurement Using Trypan Blue or AO/PI

When should you use trypan blue and when should you use acridine orange/propidium iodide to measure cell viability? »

Cell Viability and Necrosis

Cell Viability and Necrosis

Cell viability is performed using various fluorescent membrane exclusion dyes, such as PI, EB, 7AAD, and others. This assay is performed by enumerating cells in captured bright-field and fluorescent images. And Necrotic cells are detected using propidium iodide. »

Blood Samples

TNC Concentration & Viability for Clinical (Blood) Samples

Analyze fresh and processed blood and bone marrow samples without lysing: no interference from RBCs. »

Cell Size Assay

Cell Size Assay

Performing cell size measurement assay and using cell size to count cells within preset cell size parameters. For adipocytes, stem cells, Sf9 cells, dendritic cells, and others. »

Cellometer K2 Specifications

Includes
  • Cellometer K2 Instrument
  • Cellometer Software
  • Two Standard Fluorescence Optics Module
  • Power Supply
  • USB 2.0 Connection Cable
  • Phone/online applications support during set-up
Available Accessories
  • PC Laptop
Imaging Performance Cell Size: 4-90 microns
Conc. Range: 105 – 107 cells/ml
Brightfield imaging, fluorescent imaging and pattern-recognition software to quickly and accurately decluster, identify and count individual cells.
Instrument Specifications Weight: 23.0 lbs. (10.4 kg)
Dimensions: Width: 6.0” (15.2 cm), Depth: 8.5” (21.6 cm), Height: 14.0” (35.6 cm)
Input to Power Adapter: 100-240 VAC, 50/60 Hz, 1.0A
Output to Instrument: 12 VDC, 3.34A
PC / Laptop Minimum Requirements: (If purchasing Cellometer without PC laptop)
  • Windows 10, 64-bit

  • Intel® Core i7, 2.0 GHz processor

  • 16GB RAM (or higher)

  • NVIDIA P1000 GPU (equivalent or better)

Available Fluorescence Optics Modules Excitation / Emission: 470nm/535nm
Example Fluorophores:

  • Acridine Orange
  • CFDA
  • FITC

Excitation / Emission: 540nm/660nm
Example Fluorophores:

  • Propidium Iodide (PI)
  • Ethidium Bromide
  • 7-AAD

Customer Testimonials

Worth the investment

This instrument [Cellometer K2] is definitely worth the investment. It has a plethora of uses and now serves as the go-to instrument for not only counting cells but for measuring the titer of baculovirus-infected insect cells. Additionally, the customer service representatives are very helpful.


Worth the investment

This instrument [Cellometer K2] is definitely worth the investment. It has a plethora of uses and now serves as the go-to instrument for not only counting cells but for measuring the titer of baculovirus-infected insect cells. Additionally, the customer service representatives are very helpful.

Consistent and dependable

We use Cellometer K2 for cell counting. This is so convenient and easy to use. Each slide can be used for two different cell lines and the results show the total number of cells, the number of live cells and dead cells. So we can use this for a lot of experiments which are based on cell numbers and make our results more consistent and dependable.


Shan Lu

Laboratory of Cellular & Molecular Radiation Oncology, MGH

Consistent and dependable

We use Cellometer K2 for cell counting. This is so convenient and easy to use. Each slide can be used for two different cell lines and the results show the total number of cells, the number of live cells and dead cells. So we can use this for a lot of experiments which are based on cell numbers and make our results more consistent and dependable.

Shan Lu

Laboratory of Cellular & Molecular Radiation Oncology, MGH

Reproducible results

In comparison to the standard method for manual cell counting, Cellometer K2 helps me in counting cells efficiently and quickly with reproducible results. I use it mainly for assessing cell vitality by trypan blue exclusion assay.


Reproducible results

In comparison to the standard method for manual cell counting, Cellometer K2 helps me in counting cells efficiently and quickly with reproducible results. I use it mainly for assessing cell vitality by trypan blue exclusion assay.

This machine has transformed the way I culture

This machine [Cellometer K2] has transformed the way I culture. No longer do I have to spend hours out of my day to manually could cells. It has changed the way I see cell culture from a menial chore to something I look forward to every day. Cheers NEXCELOM for a job well done!!!!


This machine has transformed the way I culture

This machine [Cellometer K2] has transformed the way I culture. No longer do I have to spend hours out of my day to manually could cells. It has changed the way I see cell culture from a menial chore to something I look forward to every day. Cheers NEXCELOM for a job well done!!!!

Allowed us to enter a new field of cell culture

The K2 has allowed us to enter a new field of cell culture with best practices already in place. Not having to optimize the use of a cell counter will save us a lot of time.


Zachary Schools

Allowed us to enter a new field of cell culture

The K2 has allowed us to enter a new field of cell culture with best practices already in place. Not having to optimize the use of a cell counter will save us a lot of time.

Zachary Schools

Very good alternative to flow cytometer

Cellometer K2 is very compact, reliable and easy to use. Our lab uses this counter to count live and dead cells for our experiments. It is a very good alternative to flow cytometer for cell cycle and apoptosis studies.


Stanford University

Very good alternative to flow cytometer

Cellometer K2 is very compact, reliable and easy to use. Our lab uses this counter to count live and dead cells for our experiments. It is a very good alternative to flow cytometer for cell cycle and apoptosis studies.

Stanford University

Easy to use

Cellometer [K2] is easy to use. I like that the program can calculate transfection efficiency, one of the things that we do on a normal basis in our lab but we were not able to quantify in the past.


Easy to use

Cellometer [K2] is easy to use. I like that the program can calculate transfection efficiency, one of the things that we do on a normal basis in our lab but we were not able to quantify in the past.

This has made our life much easier.

We really like this instrument [Cellometer K2]. We were counting cells manually until now. This has made our life much easier.


MD Anderson Cancer Center

This has made our life much easier.

We really like this instrument [Cellometer K2]. We were counting cells manually until now. This has made our life much easier.

MD Anderson Cancer Center

Quicker and more accurate

Cell counting is not only quicker and more accurate [with Cellometer K2] but assays that previously required a flow cytometer, such as Annexin V/PI apoptosis analysis, can now be completed in our lab!


Quicker and more accurate

Cell counting is not only quicker and more accurate [with Cellometer K2] but assays that previously required a flow cytometer, such as Annexin V/PI apoptosis analysis, can now be completed in our lab!

Fancy and compact

The design of the instrument is very fancy and compact. It can have its own screen or you can use a separate screen. Initially, our lab was using the manual method of cell counting by microscope. But [Cellometer] K2 from Nexcelom has made our life easy and provided reproducible counting.


Fancy and compact

The design of the instrument is very fancy and compact. It can have its own screen or you can use a separate screen. Initially, our lab was using the manual method of cell counting by microscope. But [Cellometer] K2 from Nexcelom has made our life easy and provided reproducible counting.

Reliable machine

The instrument [Cellometer K2] is being used in our lab for counting different types of immune cells, mostly neutrophils (but enucleated cells as well). It has been a reliable machine, no problems in general.


The University of Alabama at Birmingham

Reliable machine

The instrument [Cellometer K2] is being used in our lab for counting different types of immune cells, mostly neutrophils (but enucleated cells as well). It has been a reliable machine, no problems in general.

The University of Alabama at Birmingham

Convenience and speed

The convenience and speed of our Nexcelom K2 and Vision Cellometers allowed us to dramatically scale up our processing of mononuclear cells. Our sales and technical representatives have always been helpful and responsive to any inquiries.


Stephanie Sieja

Convenience and speed

The convenience and speed of our Nexcelom K2 and Vision Cellometers allowed us to dramatically scale up our processing of mononuclear cells. Our sales and technical representatives have always been helpful and responsive to any inquiries.

Stephanie Sieja

Simple and intuitive

[Cellometer] K2 allows us for a faster, reliable and more accurate cell counts that our lab uses so frequently. The process itself is simple and intuitive compared to the other machine we used to have. Such a great improvement!


Hanh Chi Do

Northwestern University

Simple and intuitive

[Cellometer] K2 allows us for a faster, reliable and more accurate cell counts that our lab uses so frequently. The process itself is simple and intuitive compared to the other machine we used to have. Such a great improvement!

Hanh Chi Do

Northwestern University

Customer Publications using Cellometer K2

Our customers include: