Celigo assists in optimizing CHO cells for biopharmaceutical production

The Novo Nordisk Center for Biosustainability (Denmark) set out to improve the efficiency of Chinese hamster ovary (CHO)-cell based production of non-monoclonal antibody, therapeutic glycoproteins designed to serve as biopharmaceuticals. To optimize the growth and production capacities of these CHO cells, the scientists looked at: lipid-based transfection, cell cultivation, cell counting, and antibody-independent product titer. Different growth and transfection parameters were investigated to see which yielded the highest growth profiles and production capacities. The Celigo was used in combination with Hoechst and propidium iodide to count the cells in 96-well format. The system developed here miniaturized the process and allowed [...]

96- and 384-well Ultra-Low Adhesion Round-Bottom Multi-Well Plates now Available

384-well Ultra-low attachment treatedround bottom multi-well plates 96-well Ultra-low attachment treated round bottom multi-well plates Nexcelom has just released two new products: Ultra-low adhesion  round-bottom plates in 96-well and 384-well formats that are specifically designed for working with 3D tumor spheroids. These clear wall, clear bottom plates were validated with U87 MG cells and tracked using a bright-field imaging technique on the Celigo Image Cytometer. These plates are ideal for single spheroid drug screening assays and can also be used to investigate spheroid invasion with the addition of Matrigel. You can read more about the plates [...]